Distribution of Representative Oligotrophic Bacteria in a Pelagic Marine Environment (Ligurian Sea) by in situ Hybridization with rRNA-targeted, Fluorescently Labelled Oligonucleotides
The distribution of specific autochthonous oligotrophic bacteria in a surface layer (30 m depth) of a pelagic seawater station (DYFAMED, 28 miles off-shore, in the northwestern Mediterranean Sea) was studied by in situ hybridization with rRNA-targeted fluorescently labelled oligonucleotide probes (FISH). The data reported here extend a previous analysis of 16S rDNA clone libraries obtained by PCR from enriched oligotrophic bacterial populations in order to relate the previous enrichment results to population abundance and diversity in the original sample. For such a purpose, the frequency of matching of six previously designed group- and clone-specific probes with the naturally occurring 16S rRNA sequences was analysed The results of in situ hybridization with the rRNA-targeted fluorescently labelled oligonucleotide probes support reports of Vibrio and Rhodobacter groups in the surface layer. The high percentage of coverage of Vibrio- and Rhodobacter- specific probes (24 and 19%, respectively) resulting from in situ experiments indicates the suitability of the previously described method to obtain enrichment of numerically representative autochthonous marine bacteria.
KeywordsMarine Bacterium Bacterioplankton Community Oligotrophic Bacterium Coastal Zone Colour Scanner Aquat Microb Ecol
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- Boyle M, Ahla T, Molin S (1995) Application of a strain-specific rRNA oligonucleotide probe targeting Pseudomonas fluorescens Ag1 in a mesocosm study of bacterial release into the environment. Appl Environ Microbiol 61:1384–1390Google Scholar
- Giuliano L, De Domenico M, De Domenico E, Höfle MG, Yakimov MM (1998) Identification of culturable oligotrophic bacteria within naturally occurring bacterioplankton communities of the Ligurian Sea by 16S rRNA Sequencing and Probing. Microb Ecol 37:77–85Google Scholar
- Lane DJ (1991) 16/23 rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. John Wiley and Sons, Chichester, UK, pp 115–175Google Scholar
- Larsen N, Overbeek R, McCaughey MJ, Woese CR (1994) The ribosomal RNA database project (RDP). Nucleic Acid Res 24:82–85Google Scholar
- Minas HJ, Minas M (1990) New production considerations in the Mediterranean Sea. EOS 71:1395–1396Google Scholar
- Poulen LK, Ballard G, Stahl DA (1993) Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol 59:1354–1360Google Scholar
- Prieur L (1981) Heterogéneité spatio-temporelle dans le bassin liguro-provençal en 1981–1982. Rapp Comm Int Mer Méditerr. 27:177–179Google Scholar