Abstract
The distribution of specific autochthonous oligotrophic bacteria in a surface layer (30 m depth) of a pelagic seawater station (DYFAMED, 28 miles off-shore, in the northwestern Mediterranean Sea) was studied by in situ hybridization with rRNA-targeted fluorescently labelled oligonucleotide probes (FISH). The data reported here extend a previous analysis of 16S rDNA clone libraries obtained by PCR from enriched oligotrophic bacterial populations in order to relate the previous enrichment results to population abundance and diversity in the original sample. For such a purpose, the frequency of matching of six previously designed group- and clone-specific probes with the naturally occurring 16S rRNA sequences was analysed The results of in situ hybridization with the rRNA-targeted fluorescently labelled oligonucleotide probes support reports of Vibrio and Rhodobacter groups in the surface layer. The high percentage of coverage of Vibrio- and Rhodobacter- specific probes (24 and 19%, respectively) resulting from in situ experiments indicates the suitability of the previously described method to obtain enrichment of numerically representative autochthonous marine bacteria.
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Giuliano, L., De Domenico, M., Yakimov, M.M. (2001). Distribution of Representative Oligotrophic Bacteria in a Pelagic Marine Environment (Ligurian Sea) by in situ Hybridization with rRNA-targeted, Fluorescently Labelled Oligonucleotides. In: Faranda, F.M., Guglielmo, L., Spezie, G. (eds) Mediterranean Ecosystems. Springer, Milano. https://doi.org/10.1007/978-88-470-2105-1_24
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DOI: https://doi.org/10.1007/978-88-470-2105-1_24
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