Abstract
Polyethylene glycol based hydrogels are being considered as tissue substitutes. Here we describe the preparation of synthetic collagen-mimetic material that are stirrer than collagen but that like collagen allow for both cell encapsulation and cell growth on the surface. These materials are poloxamine based hydrogels with and without collagen; poloxamine is a four-arm PEO-PPO block copolymer derivative, Tetronic™ 107.
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References
Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24:4337–4351
Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Del Rev 54:3–12
Moghimi SM, Hunter AC (2000) Poloxamers and poloxamines in nanoparticle engineering and experimental medicine. TIBTECH 18:412–420
Aliabadi HM, Lavasanifar A (2006) Polymeric micelles for drug delivery. Exp Op Drug Del 3:139–162
Rassing J, Atwood D (1983) Ultrasonic velocity and light-scattering studies of polyoxyethylene-polyoxypropylene-polyoxyethylene copolymer Pluronic F127 in aqueous solution. Int J Pharm 13:47–55
Vadnere M, Amidon GL, Lindenbaum S et al (1984) Thermodynamic studies on gel—sol transition of some Pluronic polyols. Int J Pharm 22:207–218
Wang P, Johnston TP. Kinetics of sol-gel transition for poloxamer polyols. J Appl Polym Sci 43:283–292
Mortensen K (1992) Phase behavior of poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock-copolymer dissolved in water. Europhys Lett 19:599–604
Reeve L (1997) The poloxamers: their chemistry and medical applications. In: Domb A, Kost Y, Wiseman D (ed) Handbook of Biodegradble Polymers (Drug Targeting and Delivery, vol. 7), Harwood Academic Publishers, London, Great Britain
Pec EA, Wout ZG, Johnston TP (1992) Biologicalactivity of urease formulated in poloxamer 407 after intraperitoneal injection in the rat. J Pharm Sci 81:626–630
Grindel JM, Jaworski T, Piraner O, Emanuele RM, Balasubramanian M (2002) Distribution, metabolism, and excretion of a novel surface-active agent, purified poloxamer 188, in rats, dogs, and humans. J Pharm Sci 91:1936–1947
Batrakova EV, Li S, Li Y, Alakhov VYu, Elmquist WF, Kabanov AV (2004) Distribution kinetics of a micelle-forming block copolymer Pluronic P85. J Control Rel 100:389–397
Chiappetta DA, Sosnik A (2007) Poly(ethylene oxide)-Poly(propylene oxide) block copolymer micelles as drug delivery agents: Improved hydrosolubility, stability and bioavailability of drugs. Eur J Pharm Biopharm 66:303–317
Bromberg LE, Ron ES (1998) Temperature-responsive gels and thermogelling polymer matrices for protein and peptide delivery. Adv Drug Del Rev 31:197–221
Kabanov AV, Alkhov VYu (2002) Pluronic®block copolymers in drug delivery: From micellar nanocontainers to biological response modifiers. Critical Rev Therap Drug Carrier Syst 19:1–72
Kibbe AH (2000) Handbook of Pharmaceutical Excipients. American Pharmaceutical Association, Washington DC
Dong J, Chowdhry BZ, Leharne SA (2003) Surface activity of poloxamines at the interfaces between air-water and hexane-water. Colloids and Surfaces A: Physicochem Eng Aspects 212:9–17
Sosnik A, Sefton MV (2005) Semi-synthetic collagen/poloxamine matrices for Tissue Engineering. Biomaterials 26:7425–7435
Barichello JM, Morishita M, Takayama K et al (1999) Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats. Int J Pharm 184:189–198
Scherlund M, Brodin A, Malmsten M (2000) Micellization and gelation in block copolymer systems containing local anesthetics. Int J Pharm 211:37–49
Are’valo-Silva CA, Eavey, RD, Cao Y et al (2000) Internal support of tissue-engineered cartilage. Arch Otolaryng-Head Neck Surg 126:1448–1452
Kamil SH, Vacanti MP, Aminuddin BS et al (2004) Tissue Engineering of a human sized and shaped auricle using a mold. Laryngoscope 114:867–870
Cao Y, Rodriguez A, Vacanti Metal (1998) Comparative study of the use of poly(glycolic acid), calcium alginate and pluronics in the engineering of autologous porcine cartilage. J Biomater Sci Polym Ed 9:475–487
Saim, AB, Cao Y, Weng Y et al (2000) Engineering autogenous cartilage in the shape of a helix using an injectable hydrogel scaffold. Laryngoscope 110:1694–1697
Are’valo-Silva CA, Cao Y, Vacanti M et al (2000) Influence of growth factors on tissue-engineered pediatric elastic cartilage. Arch Otolaryng-Head Neck Surg 126:1234–1238
Are’valo-Silva CA, Cao Y, Weng Y et al (2001) The effect of fibroblast growth factor and transforming growth factor-fl on porcine chondrocytes and tissue-engineered autologous elastic cartilage. Tissue Engineering 7:81–88
Cao YL, Lach E, Kim TH et al (1998) Tissue-engineered nipple reconstruction. Plastic Reconst Surg 102:2293–2298
Weng Y, Cao Y, Silva CA et al (2001) Tissue-engineered composites of bone and cartilage for mandible condylar reconstruction. J Oral Maxillofacial Surg 59:185–190
Weinand C, Pomerantseva I, Neville CM et al (2006) Hydrogel-β-TCP scaffolds and stem cells for tissue engineering bone. Bone 38:555–563
Komiyama T, Nakao Y, Toyama Y et al (2004) Novel technique for peripheral nerve reconstruction in the absence of an artificial conduit. J Neurosci Methods 134:133–140
Liu VA, Jastromb WE, Bhatia SN (2002) Engineering protein and cell adhesivity using PEO-terminated triblock polymers. J Biomed Mater Res 60:126–134
Kamil SH, Eavey RD, Vacanti MP et al (2004) Tissue-engineered cartilage as a graft source for laryngotracheal reconstruction: A pig model. Arch Otolaryng-Head Neck Surg 130:1048–1051
Terada S, Yoshimoto H, Fuchs JR et al (2005) Hydrogel optimization for cultured elastic chondrocytes seeded onto a polyglycolic acid scaffold. J Biomed Mater Res 75A: 906–917
Zhou G, Liu W, Cui L et al (2005) In vivo chondrogenesis of BMSCs at nonchondrogenesis site by co-transplantation of BMSCs and chondrocytes with pluronic as biomaterial. Key Eng Mater 288–289:1–6
Chua KH, Aminuddin BS, Fuzina NH et al (2005) Insulin-Transferrin-Selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage. Eur Cells Mater 9:58–67
Ruszymah BHI, Chua K, Latif MA et al (2005) Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support. Tnt J Ped Otorhinolaryng 69:1489–1495
Roberts A, Wyslouzil, BE, Bonassar L (2005) Aerosol delivery of mammalian cells for tissue engineering. Biotech Bioeng 91:801–807
Xu X, Lou J, Tang T et al (2005) Evaluation of different scaffolds for BMP-2 genetic orthopedic tissue engineering. J Biomed Mater Res-Part B 75:289–303
Weinand I, Pomerantseva C, Neville R et al (2006) Hydrogel-fl-TCP scaffolds and stem cells for tissue engineering bone. Bone 38:555–563C
Cortiella J, Nichols JE, Kojima K et al (2006) Tissue-engineered lung: An in vivo and in vitro comparison of polyglycolic acid and pluronic F-127 hydrogel/somatic lung progenitor cell constructs to support tissue growth. Tissue Eng 12:1213–1225
Lee YJ, Kim IA, Park SA et al (2007) A tissue engineering based approach to regeneration of intervertebral disc. Key Eng Mat 342–343:397–400
Monroy A, Kojima K, Ghanem M et al (2007) Tissue engineered cartilage “bioshell” protective layer for subcutaneous implants. Int J Ped Otorhinolaryng 71:547–552
Hu H-L, Cao Y-L, Chen T-T et al (2007) Tissue engineered allogeneic carriage induces local immune privilege in rabbits. J Clin Rehab Tissue Eng Res 11:2757–2760
Idrus RBH, Hui CK, Ibrahim FW et al (2007) The expansion potential of human nasal septum chondrocytes for the formation of engineered cartilage. Science Asia 33:145–152
Jeong JH, Moon YM, Kim SO et al (2007) Human cartilage tissue engineering with pluronic and cultured chondrocyte sheet. Key Eng Mat 342–343:89–92
Vashia AV, Keramidarisa E, Abbertona KM et al (2008) Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro. Biomaterials 29:573–579
Thonhoff JR, Lou DI, Jordan PM et al (2008) Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro. Brain Res 1187:42–51
Dumortier G, Grosslord JL, Agnely F et al (2006) A review of poloxamer 407 pharmaceutical and pharmacological characteristics. Pharm Res 23:2709–2728
Cohn D, Sosnik A, Levy A (2003) Improved reverse thermo-responsive polymeric systems. Biomaterials 24:3707–3714
Cohn D, Lando G, Sosnik A et al (2006) PEO-PPO-PEO based poly (ether ester urethane)s as degradable thermo-responsive multiblock copolymers. Biomaterials 27:1718–1727
Cohn D, Sosnik A, Malal R et al (2007) Chain extension as a strategy for the development of improved reverse thermo-responsive polymers. Poym Adv Tech 18:731–736
Cohn D, Sosnik A (2003) Novel reverse thermo-responsive injectable poly(ether carbonate)s J Mat Sci Mater Med 14:175–180
Sosnik A, Cohn D (2005) Reverse thermo-responsive poly(ethylene oxide) and poly(propylene oxide) multiblock copolymers. Biomaterials 26:349–357
Sosnik A, Cohn D, San Roman J et al (2003) Crosslinkable PEO-PPO-PEO-based reverse thermo-responsive gels as potentially injectable materials. J Biomater Sci Pol Ed 14:227–239
Cohn D, Sosnik A, Garty S (2005) Smart hydrogels for in situ-generated implants. Biomacromolecules 6:1168–1175
Sosnik A, Cohn D (2004) Ethoxysilane-capped PEO-PPO-PEO triblocks: a new family of reverse thermo-responsive polymers. Biomaterials 25:2851–2858
Slavin S, Gurevitch O, Kullkarni BG et al (2006) Compositions comprising bone marrow cells, demineralized bone matrix and various site-reactive polymers for use in the induction of bone and cartilage formation, US Pt Appl #20060177387
Armstrong JK, Chowdry BZ, Snowden MJ et al (2001) The effect of pH and concentration upon aggregation transitions in aqueous solutions of poloxamine T701. Int J Pharm 229:57–66
Dong J, Chowdry BZ, Leharne SA (2003) Solubilisation of polyaromatic hydrocarbons in aqueous solutions of poloxamine T803. Colloids and Surfaces A: Physicochem Eng Aspects 246:91–98
Dong J, Armstrong J, Chowdry BZ et al (2004) Thermodynamic modelling of the effect of pH upon aggregation transitions in aqueous solutions of the poloxamine, T701. Therm Acta 417:201–206
Storm G, Belliot SO, Daemen T et al (1995) Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system. Adv Drug Del Rev 17:31–48
Redhead HM, Davis SS, Ilium L (2001) Drug delivery in poly(lactide-co-glycolide) nanoparticles surface modified with poloxamer 407 and poloxamine 908: In vitro characterisation and in vivo evaluation. J Control Rel 70:353–363
Sumide T, Tsuchiya T (2003) Effects of multipurpose solutions (MPS) for hydrogel contact lenses on gap-junctional intercellular communication (GJIC) in rabbit corneal keratocytes. J Biomed Mater Res-Part B 64:57–64
Alvarez-Lorenzo C, Gonzalez-Lopez CJ, Fernandez-Tarrio M et al (2007) Tetronic micellization, gelation and drug solubilization: Influence of pH and ionic strength. Eur J Pharm Biopharm 66:244–252
Chiappetta DA, Degrossi J, Teves S et al (2008) Triclosan-loaded poloxamine micelles for enhanced antibacterial activity against biofilm (in press)
Cellesi F, Tirelli N, Hubbell JA (2004) Towards a fully-synthetic substitute of alginate: development of a new process using thermal gelation and chemical cross-linking. Biomaterials 25:5115–5124
Cellesi F, Weber W, Fussenegger M et al (2004) Towards a fully synthetic substitute of alginate: Optimization of a thermal gelation/chemical cross-linking scheme (“tandem” gelation) for the production of beads and liquid-core capsules. Biotechn Bioeng 88:740–749
Winblade ND, Schmokel H, Baumann M et al (2002) Sterically blocking adhesion of cells to biological surfaces with a surface-active copolymer containing poly(ethylene glycol) and phenylboronic acid. J Biomed Mater Res 59:618–631
Llanos GR, Sefton MV (1993) Immobilization of poly (ethylene glycol) onto a poly (vinyl alcohol) hydrogel. 2. Evaluation of thrombogenicity. J Biomed Mater Res 27:1383–1391
McGuigan AP, Sefton MV (2006) Vascularised organoid engineered by modular assembly enables blood perfusion. Proc Natl Acad Sci USA 103:11461–11466
Lee CH, Singla A, Lee Y (2001) Biomedical applications of collagen. Int J Pharm 221:1–22
Sosnik A, Brodersen P, Sodhi RNS et al (2006) Surface study of collagen/poloxamine hydrogelsby a ‘deep freezing’ ToF-SIMS approach. Biomaterials 27:2340–2348
Sosnik A, Leung B, McGuigan AP et al Collagen/poloxamine hydrogels: Cytocompatibility of embedded HepG2 cells and surface attached endothelial cells. Tissue Eng 11:1807–1816
Margiotta MS, Robertson FS, Greco RS (1992) The adherence of endothelial cells to Dacron induces the expression of the intercellular adhesion molecule (ICAM-1). Ann Surg 216: 600–604
Cenni E, Granchi D, Ciapetti G et al (1997) Expression of adhesion molecules on endothelial cells after contact with knitted Dacron. Biomaterials 18:489–494
Granchi D, Cenni E, Verri E et al (1998) Adhesive protein expression on human endothelial cells after in vitro contact with woven Dacron. Biomaterials 19:93–98
Sosnik A, Sefton MV (2005) Poloxamine hydrogels with a quaternary ammonium modification to improve cell attachment. J Biomed Mater Res-Part A 75:295–307
Sosnik A, Sefton MV (2006) Methylation of poloxamine for enhanced cell adhesion. Biomacromolecules 7:331–338
Sosnik A, Leung BM, Sefton MV (2008) Lactoyl-poloxamine/collagen matrix for cell-containing modules. J Biomed Mater Res-Part A (in press)
Leung BM (2005) A modular vascularized Tissue Engineering construct containing smooth muscle cells and endothelial cells. M.Sc. Thesis. University of Toronto, Toronto, Canada
Leung BM, Sefton MV (2007) A modular Tissue Engineering construct containing smooth muscle cells and endothelial cells. Ann Biomed Eng 35:2039–2049
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Sosnik, A., Khan, O.F., Butler, M., Sefton, M.V. (2009). Poloxamine Hydrogels: from low Cell Adhesion Substrates to Matrices with Improved Cytocompatibility for Tissue Engineering Applications. In: Hydrogels. Springer, Milano. https://doi.org/10.1007/978-88-470-1104-5_8
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DOI: https://doi.org/10.1007/978-88-470-1104-5_8
Publisher Name: Springer, Milano
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