Chitosan-Based Beads for Controlled Release of Proteins
Chitosan is biocompatible polymer of natural origin widely investigated for applications in drug delivery and regenerative medicine. In this paper, Chitosan’s capability of forming water gelling beads in the presence of non-toxic polyanion was exploited for the loading of two model proteins. Human Serum Albumin (HSA) and porcine trypsin were successfully loaded into Chitosan-tripolyphosphate (TPP) beads. Both proteins were highly incorporated when the crosslinking process was allowed to occur for 24 h at RT. The release profiles of the two proteins were compared and the faster diffusion of trypsin was associated to its smaller molecular weight. Moreover, in vitro degradation experiments, aimed at mimicking the physiological degradation pattern of the beads displayed a complete degradation of the material in almost 30 days. Certainly, the preliminary experimental data acquired in the present work represent a starting study for the promising application of Chitosan-TPP beads for the controlled release of proteins of therapeutic interest.
KeywordsHuman Serum Albumin Control Release Encapsulation Efficiency Chitosan Bead Porcine Trypsin
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- Steinbiichel A (2002) Biopolymers. Vandamme EJ (ed) Volume 5. Polysaccharides~I: polysaccharides from eukaryotes and Volume 6. Polysaccharides II: polysaccharides from prokaryotes. Wiley-VCH Verlag GmbH, Weinheim, GermanyGoogle Scholar