Hydrogels pp 111-120 | Cite as

Chitosan-Based Beads for Controlled Release of Proteins

  • Mamoni Dash
  • Anna Maria Piras
  • Federica Chiellini


Chitosan is biocompatible polymer of natural origin widely investigated for applications in drug delivery and regenerative medicine. In this paper, Chitosan’s capability of forming water gelling beads in the presence of non-toxic polyanion was exploited for the loading of two model proteins. Human Serum Albumin (HSA) and porcine trypsin were successfully loaded into Chitosan-tripolyphosphate (TPP) beads. Both proteins were highly incorporated when the crosslinking process was allowed to occur for 24 h at RT. The release profiles of the two proteins were compared and the faster diffusion of trypsin was associated to its smaller molecular weight. Moreover, in vitro degradation experiments, aimed at mimicking the physiological degradation pattern of the beads displayed a complete degradation of the material in almost 30 days. Certainly, the preliminary experimental data acquired in the present work represent a starting study for the promising application of Chitosan-TPP beads for the controlled release of proteins of therapeutic interest.


Human Serum Albumin Control Release Encapsulation Efficiency Chitosan Bead Porcine Trypsin 
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Copyright information

© Springer-Verlag Italia, Milan 2009

Authors and Affiliations

  • Mamoni Dash
    • 1
  • Anna Maria Piras
    • 1
  • Federica Chiellini
    • 1
  1. 1.Department of Chemistry & Industrial ChemistryUniversity of PisaItaly

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