Molecular Diagnosis of Newcastle Disease Virus
Several protocols for the detection of avian paramyxovirus type 1 (APMV-1) by retrotranscription polymerase chain reaction (RT-PCR) have been published in the last decade. A technical review was presented a few years ago (Aldous et al, 2001) in which conventional (end-point) RT-PCR protocols were described. More recently, real time PCR (rPCR) protocols based on the use of hydrolysis probes, SybrGreen or LUX primers have been published (Wise et al. 2004; Pham et al. 2005; Antal et al. 2007). In the following, protocols used by the IZSVe are presented.
KeywordsAvian Influenza Newcastle Disease Virus Newcastle Disease Hydrolysis Probe Avian Paramyxovirus
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