Molecular Diagnosis of Newcastle Disease Virus

  • Giovanni Cattoli
  • Isabella Monne


Several protocols for the detection of avian paramyxovirus type 1 (APMV-1) by retrotranscription polymerase chain reaction (RT-PCR) have been published in the last decade. A technical review was presented a few years ago (Aldous et al, 2001) in which conventional (end-point) RT-PCR protocols were described. More recently, real time PCR (rPCR) protocols based on the use of hydrolysis probes, SybrGreen or LUX primers have been published (Wise et al. 2004; Pham et al. 2005; Antal et al. 2007). In the following, protocols used by the IZSVe are presented.


Avian Influenza Newcastle Disease Virus Newcastle Disease Hydrolysis Probe Avian Paramyxovirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Antal M, Farkas T, Germán P et al (2007) Real-time reverse transcription-polymerase chain reaction detection of Newcastle disease virus using light upon extension fluorogenic primers. J Vet Diagn Invest 19(4):400–404PubMedGoogle Scholar
  2. Creelan JL, Graham DA, McCullough SJ (2002) Detection and differentiation of pathogenicity of avian paramyxovirus serotype 1 from field cases using onestep reverse transcriptase-polymerase chain reaction. Avian Pathol 31(5):493–499PubMedCrossRefGoogle Scholar
  3. Aldous EW, Alexander DJ (2001) Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1) Avian Pathol 30:117–128PubMedCrossRefGoogle Scholar
  4. Pham HM, Konnai S, Usui, T et al (2005) Rapid detection and differentiation of Newcastle disease virus by real-time PCR with melting-curve analysis. Arch Virol 150(12):2429–2438PubMedCrossRefGoogle Scholar
  5. Wise MG, Suarez DL, Seal BS et al (2004) Development of a real-time reverse-transcription PCR for detection of Newcastle disease virus RNA in clinical samples. J Clin Microbiol 42(1):329–338PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Italia 2009

Authors and Affiliations

  • Giovanni Cattoli
    • 1
  • Isabella Monne
    • 2
  1. 1.Virology Department, OIE/FAO and National, Reference Laboratory for Newcastle Disease and Avian InfluenzaIstituto Zooprofilattico, Sperimentale delle VenezieLegnaro, PadovaItaly
  2. 2.OIE/FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, Research and Development LaboratoryIstituto Zooprofilattico Sperimentale delle VenezieLegnaro, PadovaItaly

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