The Phylogeny and Structural Properties of 2/2 Haemoglobins

  • David A. Vuletich
  • Juliette T. J. Lecomte
Part of the Protein Reviews book series (PRON, volume 9)


In 2002, Jonathan and Beatrice Wittenberg inspected the 42 protein sequences then known to belong to the lineage of truncated (two-on-two) globins. They pointed out that these proteins parted into three distinct phylogenetic groups. The classification allowed for the identification of essential residues surrounding the heme group and guided subsequent structural studies. It also provided clear targets for further experimental work and set the stage for analyses of gene history. In this chapter, the status of the two-on-two globin lineage is updated. It is shown that the 2002 observations withstand the test of additional sequences and structures. The two-on-two family, which has grown practically ten-fold since the original study, conforms to the group-specific mechanisms of ligand stabilisation and fold features anticipated in the seminal Wittenberg contribution.


Heme Group Exogenous Ligand Heme Pocket Geobacillus Stearothermophilus Servation Pattern 
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Copyright information

© Springer-Verlag Italia 2008

Authors and Affiliations

  • David A. Vuletich
    • 1
  • Juliette T. J. Lecomte
    • 1
  1. 1.Chemistry DepartmentThe Pennsylvania State UniversityUniversity ParkUSA

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