From O2 Diffusion into Red Blood Cells to Ligand Pathways in Globins
Jonathan and Beatrice Wittenberg have had a strong influence on much of the work done in my laboratory over the past 35 years, both indirectly through their papers and more directly through conversations and collaborations. I began reading their papers on facilitated diffusion of O2 by myoglobin (Wittemberg 1965; 1970; Riveros-Moreno and Wittenberg 1972), gas exchange in fish swim bladders (Wittenberg 1958; 1961; Wittenberg and Wittenberg 1961; Wittenberg et al. 1964) and ligand binding to leghaemoglobin (Wittenberg et al. 1972) when I was a graduate student in Quentin Gibson’s laboratory between 1968 and 1972. Then, as an independent investigator, I became involved in a number of studies, which built on their discoveries about the role of globins in O2 storage, transport, sensing and scavenging. Four of these projects are summarised in this chapter and were chosen because of the strong influence of the Wittenbergs’ work and are presented in a tribute to them and to their ideas. These studies include: (1) a demonstration that O2 uptake and release by intact red blood cells is limited by diffusion through unstirred surface layers; (2) an evaluation of the factors governing sulphide binding to Lucina pectinata HbI; (3) a comparison of symbiotic and non-symbiotic plant haemoglobins; and (4) an experimental verification that O2 enters and exits Cerebratulus lacteus Hb through an internal apolar channel.
KeywordsSperm Whale Unstirred Layer Distal Histidine Distal Pocket Ligand Pathway
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