Specific T-Cell Response to HCMV Infection

  • Giovanna Lucchini
  • Pietro Pioltelli
  • Marco Lanzetta


The first isolation of human cytomegalovirus (HCMV) in a renal transplanted patient dates back to 1965. Since then, this infection has been one of the most challenging for immunosuppressed patients. In spite of the danger represented by this herpes virus, the specific cellular response induced by it in affected humans has been detected and investigated in its deep mechanisms only recently.


HCMV Infection Specific Cellular Response HCMV Strain Autologous Peripheral Blood Mononuclear Cell Cytokine Flow Cytometry 
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  1. 1.
    Scholz M, Doerr HW, Cinatl J (2003) Human cytomegalovirus retinitis: pathogenicity, immune evasion and persistence. Trends Microbiol 11:171–178PubMedCrossRefGoogle Scholar
  2. 2.
    Raju R, Jordan S, Puliyanda S et al (2005) Cellular immune responses to Cytomegalovirus in renal transplant recipients. Am J Transplant 5:110–117CrossRefGoogle Scholar
  3. 3.
    Van Lier RA, Borst J, Vroom TM et al (1987) Tissue distribution and biochemical and functional properties of CD27, a novel T cell differentiation antigen. J Immunol 139:1589–1596PubMedGoogle Scholar
  4. 4.
    Hamman D, Baars PM, Rep MH et al (1997) Phenotypic and functional separation of memory and effectors human CD8+ T cells. J Ex Med 186:1407–1418CrossRefGoogle Scholar
  5. 5.
    De Rosa SC, Herzenberg LA, Roederer M (2001) 11-color, 13-parameter flow cytometry: identification of human naïe T-cells by phenotype, function and T-cell receptor diversity. Nat Med 7:245–249PubMedCrossRefGoogle Scholar
  6. 6.
    Van Lier RAW, Ten Berge IJ, Gamadia LE (2003) Human CD8+T-cell differentiation in response to viruses. Nat Rev Immunol 3:931–938PubMedCrossRefGoogle Scholar
  7. 7.
    Harari A, Rizzardi GP, Ellefsen K et al (2002) Analysis of HIV-1 and CMV-specific memory CD4 T-cell response during primary and chronic infection. Blood 100:1381PubMedCrossRefGoogle Scholar
  8. 8.
    Gratama JW, van Esser JW, Lamers CH et al (2001) Tetramer-based quantification of cytomegalovirus specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection. Blood 98:1358PubMedCrossRefGoogle Scholar
  9. 9.
    Gamadia LE, Rentenaar RJ, Baars P et al (2001) Differentiation of cytomegalovirus-specific CD8(+) T cells in healthy and immunosuppressed virus carriers. Blood 98:754–761PubMedCrossRefGoogle Scholar
  10. 10.
    Gamadia LE, Remmerswaal EB, Weel JF et al (2003) Primary immune responses to human CMV: a critical role for IFN-gamma-producing CD4+ T cells in protection against CMV disease. Blood 101:2686PubMedCrossRefGoogle Scholar
  11. 11.
    Dunn HS, Haney DJ, Ghanekar SA et al (2002) Dynamics of CD4 and CD8 T cell responses to Cytomegalovirus in healthy human donors. J Inf Dis 186:15–22CrossRefGoogle Scholar
  12. 12.
    Lidehall AK, Sund F, Lundberg T et al (2005) T cell control of primary and latent cytomegalovirus infections in healthy subjects. J Clin Immunol 25(5):473–481PubMedCrossRefGoogle Scholar
  13. 13.
    Engstrand M, Lidehall AK, Totterman TH et al (2003) Cellular responses to cytomegalovirus in immunosuppressed patients: circulating CD8+ T cells recognizing CMVpp65 are present but display functional impairment. Cl Exp Immunol 132:96CrossRefGoogle Scholar
  14. 14.
    Seder RA, Ahmed R (2003) Similarities and differences in CD4+ and CD8+ effector and memory T cell generation. Nat Immunol 4:835–842PubMedCrossRefGoogle Scholar
  15. 15.
    Pan J, Ju D, Wang Q et al (2001) Dexamethasone inhibits the antigen presentation of dendritic cells in MHC class II pathway. Immunol Lett 76:153–161PubMedCrossRefGoogle Scholar
  16. 16.
    Sester M, Sester U, Gartner BC (2002) Dominance of virus specific CD8 T cells in human primary cytomegalovirus infection. J Am Soc Nephrol 13:2577–2584PubMedCrossRefGoogle Scholar
  17. 17.
    Meyers JD, Flournoy N, Thomas ED (1980) Cytomegalovirus infection and specific cell-mediated immunity after marrow transplant. J Infect Dis 142:816–24PubMedGoogle Scholar
  18. 18.
    Waldrop SL, Pitcher CJ, Peterson DM et al (1997) Determination of antigen specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest 99:1739–1750PubMedCrossRefGoogle Scholar
  19. 19.
    Romero P, Cerottini JC, Waanders GA (1998) Novel methods to monitor antigen-specific cytotoxic T-cell responses in cancer immunotherapy. Molec Med Today 4:305CrossRefGoogle Scholar
  20. 20.
    Harari A, Zimmerli SC, Pantaleo G (2004) Cytomegalovirus (CMV)-specific cellular immune responses. Hum Immunol 65:500–506PubMedCrossRefGoogle Scholar
  21. 21.
    Kern F, Surel IP, Brock C et al (1998) T cell epitope mapping by flow cytometry. Nat Med 4:975–978PubMedCrossRefGoogle Scholar
  22. 22.
    Kern F, Faulhaber N, Frommel C et al (2000) Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. Eur J Immunol 30:1676–1682PubMedCrossRefGoogle Scholar
  23. 23.
    Maecker HT, Dunn HS, Suni MA et al (2001) Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods 255:27–40PubMedCrossRefGoogle Scholar
  24. 24.
    Lozza L, Lilleri D, Percivalle E et al (2005) Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV infected immature dendrite cells. Eur J Immunol 35:1795–1804PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Italia 2007

Authors and Affiliations

  • Giovanna Lucchini
    • 1
  • Pietro Pioltelli
    • 2
  • Marco Lanzetta
    • 3
    • 4
    • 5
    • 6
    • 7
  1. 1.Hand Surgery and Microsurgery UnitSan Gerardo Hospital MonzaMilanItaly
  2. 2.Haematology UnitSan Gerardo Hospital MonzaMilanItaly
  3. 3.Italian Institute of Hand Surgery MonzaMilanItaly
  4. 4.Hand Surgery and Reconstructive Microsurgery UnitSan Gerardo HospitalMonza, MilanItaly
  5. 5.University of Milan-BicoccaMilanItaly
  6. 6.University of CanberraAustralia
  7. 7.International Hand and CompositeTissue Allograft SocietyAustralia

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