Synthesis of Chitosan-Based Nanomaterials

  • Vinod Saharan
  • Ajay Pal
Part of the SpringerBriefs in Plant Science book series (BRIEFSPLANT)


Basic concept of this method is to convert the material into microscopic droplets through emulsion and further stabilize the particles into nano size by cross-linking. Water-in-oil (w/o) emulsion is prepared under mechanical stirring by mixing chitosan aqueous solution into oil. A suitable surfactant is used to achieve the stable emulsion. The stable emulsion is cross-linked by using an appropriate cross-linking agent such as glutaraldehyde, formaldehyde, genipin, glyoxal, sulfuric acid, poloxamer, etc. For efficient cross-linking, overnight stirring of the reaction should be performed. Stirring rate is based on the volume of the reaction and desired physical properties of nanoformulation. A translucent solution obtained from overnight stirring is then filtered and washed repeatedly with n-hexane followed by alcohol to remove oil. The surfactant can be removed by precipitation with appropriate salts like CaCl2 followed by centrifugation. Resulted precipitates can then be lyophilized to get a fine dry nanomaterial (Akbuga and Durmaz 1994). Method is explained in Fig. 2.1.


Dynamic Light Scattering Stable Emulsion Chitosan Solution Chitosan Nanoparticles Sodium Tripolyphosphate 
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© The Author(s) 2016

Authors and Affiliations

  • Vinod Saharan
    • 1
  • Ajay Pal
    • 2
  1. 1.Department of Molecular Biology and BiotechnologyMaharana Pratap University of Agriculture and TechnologyUdaipurIndia
  2. 2.Department of Chemistry and Biochemistry College of Basic Sciences and HumanitiesChaudhary Charan Singh Haryana Agricultural UniversityHisarIndia

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