Seed Priming for Abiotic Stress Tolerance: An Overview



Heat, drought, cold, and salt stress are some of the major kinds of stresses that crops usually face under adverse weather or soil conditions. Abiotic stresses are often interrelated, either individually or in combination; they cause morphological, physiological, biochemical, and molecular changes that affect plant growth and development and ultimately yield. Various methodologies are in vogue to evolve stress-tolerant varieties either through conventional breeding or through transgenics. However, alternatively, more simple and economical practices are also in race to address this problem. Seed priming is one such farmer’s friendly techniques recommended by many researchers for better crop stand establishment and growth even under adverse conditions. The present chapter deals with different seed priming methods and their scope in mitigating stress effects. Besides hydro-, osmo-, and halopriming, the relevance of nutrient priming and redox priming techniques for stress tolerance was also discussed. Further, how seed “priming-induced” biochemical and molecular changes regulate stress tolerance was amply explained in the light of the latest research work carried in this direction. Although the phenomenon of “priming-induced” stress tolerance appears complex, the present-day advanced techniques like proteomics, genomics, metabolomics, and transcriptomics made the task much simpler to understand these events clearly at subcellular level. Since priming mimics similar events happening under stress, the same can be exploited as a model system to decipher pathways that contribute stress tolerance.


Cold Stress Late Embryogenesis Abundant Glycine Betaine Abiotic Stress Tolerance Seed Priming 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Afzal I, Rauf S, Basra SMA, Murtaza G (2008) Halopriming improves vigor metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environ 54(9):382–388Google Scholar
  2. Ahment K, Murat U, Ali Riza D (2007) Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress. Acta Physiol Plant 29:503–508CrossRefGoogle Scholar
  3. Ait Barka E, Nowak J, Clement C (2006) Enhancement of chilling resistance of inoculated grapevine plantlets with a plant growth promoting rhizobacterium Burkholderia phytofirmans strain PsJN. Appl Environ Microbiol 72(11):7246–7252PubMedPubMedCentralCrossRefGoogle Scholar
  4. Ali SKZ, Sandhya V, Minakshi Grover Kishore N, Venkateswara Rao L, Venkateswarlu B (2009) Pseudomonas sp strain AKM–P6 enhances tolerance of sorghum seedlings to elevated temperatures. Biol Fertil Soils 46:45–55CrossRefGoogle Scholar
  5. Al-Mudaris MA, Jutzi SC (1999) The influence of fertilizer based seed priming treatment on emergence and seedling growth of Sorghum bicolor and Pennisetum glaucum in pot trials under greenhouse conditions. J Agron Crop Sci 182:135–141CrossRefGoogle Scholar
  6. Ansari O, Choghazardi HR, Sharif-Zadeh F, Nazarli H (2012) Seed reserve utilization and seedling growth of treated seeds of mountain ray (Secale montanum) as affected by drought stress. Cercet Agronomice Moldova 2(150):43–48Google Scholar
  7. Arrvin MJ, Kazemi Poor N (2003) Response of onion cultivation to drought and salinity stress at germination stage and seed priming by chemicals to improve germination. Iran J Hortic Sci Technol 4:95–104Google Scholar
  8. Atreya A, Vartak V, Bhargava S (2009) Salt priming improves tolerance to desiccation stress and to extreme salt stress in Bruguiera cylindrica. Int J Integr Biol 6(2):68–73Google Scholar
  9. Baek KH, Skinner DZ (2003) Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines. Plant Sci 165:1221–1227CrossRefGoogle Scholar
  10. Bano A, Fatima M (2009) Salt tolerance in Zea mays (L) following inoculation with Rhizobium and Pseudomonas. Biol Fertil Soils 45:405–413CrossRefGoogle Scholar
  11. Begum MM, Sariah M, Puteh AB, Zainal Abidin MA, Rahman MA, Siddiqui Y (2010) Field performance of bio primed seeds to suppress Colletotrichum truncatum causing damping off and seedling strand of soybean. Biol Control 53:18–23CrossRefGoogle Scholar
  12. Behnamnia M, Kalantari M, Rezanejad F (2009) Exogenous application of brassinosteriods alleviates drought induced oxidative stress in Lycopersicon esculentum. Gen Appl Plant Physiol 35:22–24Google Scholar
  13. Besford RT, Richardson CM, Campos JL, Tiburcio AF (1993) Effects of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves. Planta 189:201–206CrossRefGoogle Scholar
  14. Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066PubMedPubMedCentralCrossRefGoogle Scholar
  15. Bewley JD, Black M (1982) Physiology and biochemistry of seeds, vol 2, Viability Dormancy and Environmental Control. Springer, BerlinGoogle Scholar
  16. Bourgne S, Job C, Job D (2000) Sugarbeet seed priming: solubilization of the basic subunit of 11-S globulin in individual seeds. Seed Sci Res 10:153–156Google Scholar
  17. Breusegem FV, Vranova E, Dat JF, Inze D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414CrossRefGoogle Scholar
  18. Bruce TJA, Matthes MC, Napier JA, Pickett JA (2007) Stressful “memories” of plants: evidence and possible mechanisms. Plant Sci 173:603–608CrossRefGoogle Scholar
  19. Burnell JN (1990) Immunological study of carbonic anhydrase in C and C plants using antibodies to maize cytosolic and spinach chloroplastic carbonic anhydrase. Plant Cell Physiol 31:423–427Google Scholar
  20. Cakmak I (2000) Possible role of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205CrossRefGoogle Scholar
  21. Cakmak I, Marschner H (1988) Increase in membrane permeability and exudation in roots of zinc deficient plants. J Plant Physiol 132:356–361CrossRefGoogle Scholar
  22. Catusse J, Meinhard J, Job C, Strub J, Fischer U, Pestsova E, West- hoff P, Van Dorsselaer A, Job D (2011) Proteomics reveals potential biomarkers of seed vigor in sugarbeet. Proteomics 11:1569–1580PubMedCrossRefGoogle Scholar
  23. Cava F, Zafra O, da Costa MS, Berenguer J (2008) The role of the nitrate respiration element of Thermus thermophilus in the control and activity of the denitrification apparatus. Environ Microbiol 10:522–533PubMedCrossRefGoogle Scholar
  24. Chouliaras V, Therios I, Molassiotis A, Patakas A, Diamantidis G (2004) Effect of iron deficiency on gas exchange and catalase and peroxidase activity in citrus. J Plant Nutr 27:2085–2099CrossRefGoogle Scholar
  25. Christensen JH, Christensen OB (2007) A summary of the PRUDENCE model projections of changes in European climate by the end of this century. Clim Change 81:7–30CrossRefGoogle Scholar
  26. Christou A, Manganaris GA, Papadopoulos I, Fotopoulos V (2013) Hydrogen sulfide induces systemic tolerance to salinity and non-ionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathways. J Exp Bot 64(7):1953–1966PubMedPubMedCentralCrossRefGoogle Scholar
  27. Conrath U, Beckers GJM, Flors V, Garcia-Agustin P, Jakab G, Mauch F, Newman MA, Pieterse CMJ, Poinssot B, Pozo MJ, Pugin A, Schaffrath U, Ton J, Wendehenne D, Zimmerli L, Mauch-Mani B (2006) Priming: getting ready for battle. Mol Plant Microbe Interact 19:1062–1071PubMedCrossRefGoogle Scholar
  28. Corbineau F, Ozbingol N, Vineland D, Come D (2000) Improvement of tomato seed germination by osmopriming as related to energy metabolism. In: Black M, Bradford KJ, Vasquez-Ramos J (eds) Seed biology advances and applications: proceedings of the sixth international workshop on seeds. CABI Cambridge, Merida, pp 467–474CrossRefGoogle Scholar
  29. Cuartero J, Bolarin MC, Asins MJ, Moreno V (2006) Increasing salt tolerance in tomato. J Exp Bot 57:1045–1058PubMedCrossRefGoogle Scholar
  30. Davies PJ (2004) The plant hormones: their nature occurrence and function. In: Davies PJ (ed) Plant hormones biosynthesis signal transduction action. Kluwer, DordrechtGoogle Scholar
  31. Derrick JW, Ryan MH (1998) Influence of seed phosphorus content on seedling growth in wheat: implications for organic and conventional farm management in South East Australia. Biol Agric Hortic 16:223–237CrossRefGoogle Scholar
  32. Downie B, Gurusinghe S, Dahal P, Thacker RR, Snyder JC, Nonogaki H, Yim K, Fukanaga K, Alvarado V, Bradford KJ (2003) Expression of a GALACTINOL SYNTHASE gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented. Plant Physiol 131:1347–1359PubMedPubMedCentralCrossRefGoogle Scholar
  33. Dwivedi R, Snehi Takkar PN (1974) Ribonuclease activity as an index of hidden hunger of zinc in crops. Plant and Soil 40:173–181CrossRefGoogle Scholar
  34. Farhoudi R, Sharifzadeh F (2006) The effects of NaCl priming on salt tolerance in canola (Brassica napus L) seedlings grown under saline conditions. Indian J Crop Sci 1(1–2):74–78Google Scholar
  35. Farooq M, Basra SMA, Hussain M, Rehman H, Saleem BA (2007) Incorporation of polyamines in the priming media enhances the germination and early seedling growth in hybrid sunflower (Helianthus annuus L). Int J Agric Biol 9:868–872Google Scholar
  36. Fincher GB (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Biol 40:305–346CrossRefGoogle Scholar
  37. Frankow-Lindberg BE (2001) Adaptation to winter stress in nine white clover populations: changes in non-structural carbohydrates during exposure to simulated winter conditions and ‘spring’ regrowth potential. Ann Bot 88:745–751CrossRefGoogle Scholar
  38. Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerekhove J, Job D (2004) Proteomics of Arabidopsis seed germination and priming. In: Nicholas G (ed) The biology of seeds: recent advances. CABI, Cambridge, pp 199–209Google Scholar
  39. Gnan Y, Hu J, Wang X, Shao C (2009) Seed priming with chitosan improves maize germination and seedling growth in relation to physiological enhances under low temperature stress. J Zhejiang Univ Sci B 10(6):427–433CrossRefGoogle Scholar
  40. Harris D (2006) Development and testing of onfarm seed priming. Adv Agron 90:129–178CrossRefGoogle Scholar
  41. Hasanuzzaman M, Nahar K, Fujita M (2013) Extreme temperatures oxidative stress and antioxidant defense in plants. In: Vahdati K, Leslie C (eds) Abiotic stress – plant responses and applications in agriculture. In Tech, Rijeka, pp 169–205Google Scholar
  42. Hil R (1954) The cytochrome b component of chloroplasts. Nature 174:501CrossRefGoogle Scholar
  43. Hopkins WG (1995) Introduction to plant physiology, 4th edn. Wiley, New YorkGoogle Scholar
  44. Imran MA, Mehmood V, Römheld G, Neumann (2013) Nutrient seed priming improves seedling development and increases grain yield of maize exposed to low root zone temperatures during early growth. Eur J Agron 49:141–148CrossRefGoogle Scholar
  45. Jakab G, Ton J, Flors V, Zimmerli L, Me’traux JP, Mauch-Mani B (2005) Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Plant Physiol 139:267–274PubMedPubMedCentralCrossRefGoogle Scholar
  46. Janmohammadi M, Moradi Dezfuli P, Sharifzadeh F (2008) Seed invigoration techniques to improve germination and early growth of inbred line of maize under salinity and drought stress. Gen Appl Plant Physiol 34:215–226Google Scholar
  47. Jisha KC, Puthur JT (2015) Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L) Wilczek. Protoplasma. doi: 10.1007/s00709-015-0804-7 PubMedGoogle Scholar
  48. Kasper TC, Bland WI (1992) Soil temperature and root growth. Soil Sci 154:290–299CrossRefGoogle Scholar
  49. Kester ST, Geneve RL, Houtz RL (1997) Priming and accelerated ageing effect L-isoaspartylmethyltransferase activity in tomato (Lycopersicon esculentum Mill) seed. J Exp Bot 48:943–949CrossRefGoogle Scholar
  50. Khajeh-Hosseini M, Powell AA, Bimgham IJ (2003) The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci Technol 31:715–725CrossRefGoogle Scholar
  51. Kim TH, Bohmer M, Hu H, Nishimura N, Schroeder JI (2010) Guard cell signal transduction network: advances in understanding abscisic acid CO2 and Ca2+ signaling. Annu Rev Plant Biol 61:561–591PubMedPubMedCentralCrossRefGoogle Scholar
  52. Larbi A, Abadía A, Morales F, Abadía J (2004) Fe resupply to Fe-deficient sugar beet plants leads to rapid changes in the violaxanthin cycle and other photosynthetic characteristics without significant de novo chlorophyll synthesis. Photosynth Res 79:59–69PubMedCrossRefGoogle Scholar
  53. Lee GJ, Pokala N, Vierling E (1995) Structure and in vitro molecular chaperone activity of cytosolic small heat shock proteins from pea. J Biol Chem 270:10432–10438PubMedCrossRefGoogle Scholar
  54. Lemanceau P, Bauer P, Kraemer S, Briat JF (2009) Iron dynamics in the rhizosphere as a case study for analyzing interactions between soils plants and microbes. Plant Soil 321:513–535CrossRefGoogle Scholar
  55. Li F, Wu X, Tsang E, Cutler AJ (2005) Transcriptional profiling of imbibed Brassica napus seed. Genomics 86:718–730PubMedCrossRefGoogle Scholar
  56. Li Z, Lu GY, Zhang YK, Zou CS, Cheng Y, Zheng PY (2010) Improving drought tolerance of germinating seeds by exogenous application of GA3 in rapeseed. Seed Sci Technol 38:432–440CrossRefGoogle Scholar
  57. Ligterink WJ, Kodde M, Lammers H, Dassen AHM, van der Geest RA, De Maagd RA, Hilhorst HWM (2007) Stress-inducible gene expression and its impact on seed and plant performance: a microarray approach. In: Adkins S, Ashmore S, Navie SC (eds) Seeds: biology development and ecology. CAB International, Wallingford, pp 139–148Google Scholar
  58. Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc iron manganese and copper. Soil Sci Soc Am J 42:421–428CrossRefGoogle Scholar
  59. Madlung A, Comai L (2004) The effect of stress on genome regulation and structure. Ann Bot 94:481–495PubMedPubMedCentralCrossRefGoogle Scholar
  60. Marcia DP, Denise C, Luiz A, Eusdarardo FA (2009) Primed carrot seeds performance under water and temperature stress. Sci Agric 662:174–179Google Scholar
  61. Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, San DiegoGoogle Scholar
  62. McDonald MB (2000) Seed priming. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, Sheffield, pp 287–325Google Scholar
  63. Mehmet Y, Digdem K (2008) Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. Afr J Biotechnol 7(13):2156–2162Google Scholar
  64. Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2009) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467PubMedCrossRefGoogle Scholar
  65. Mittler R (2002) Oxidative stress antioxidants and stress tolerance. Trends Plant Sci 9:405–410CrossRefGoogle Scholar
  66. Moosavi A, Tavakkol Afshari R, Sharif-Zadeh F, Aynehband A (2009) Seed priming to increase salt and drought stress tolerance during germination in cultivated species of Amaranth. Seed Sci Technol 37:781–785CrossRefGoogle Scholar
  67. Muhammad F, Abdul W, Dong JL (2009) Exogenous applied polyamines increases drought tolerance of rice by improving leaf water status photosynthesis and membrane properties. Acta Physiol Plant 31:937–945CrossRefGoogle Scholar
  68. Munns RA, Gardrer ML, Rawson HM (1988) Growth and development in NaCl treated plants II Do Na+ or Cl concentrations in dividing or expanding tissue determine growth in barley. Aust J Plant Physiol 15:529–540CrossRefGoogle Scholar
  69. Nemeth M, Janda T, Hovarth E, Paldi E, Szali G (2002) Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Sci 162:569–574CrossRefGoogle Scholar
  70. Niranjan RS, Shetty NP, Shetty HS (2004) Seed bio- priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew. Int J Pest Manag 50(1):41–48CrossRefGoogle Scholar
  71. Obata T, Fernie AR (2012) The use of metabolomics to dissect plant responses to abiotic stresses. Cell Mol Life Sci 69:3225–3243PubMedPubMedCentralCrossRefGoogle Scholar
  72. Parera CA, Cantliffe DJ (1994) Presowing seed priming. Hortic Rev 16:109–114Google Scholar
  73. Passam HC, Kakouriotis D (1994) The effect of osmo conditioning on germination and emergence and early plant growth of cucumber under saline conditions. Sci Hortic 57:233–240CrossRefGoogle Scholar
  74. Perur NG, Smith RL, Wiebe HH (1961) Effect of iron chlorosis on protein fraction on corn leaf tissue. Plant Physiol 36:736–739PubMedPubMedCentralCrossRefGoogle Scholar
  75. Pill WG, Freett JJ, Morneau DC (1991) Germination and seedling emergence of primed tomato and asparagus seeds under adverse conditions. Hortic Sci 26:160–1162Google Scholar
  76. Pregitzer KS, King JS (2005) Effects of soil temperature on nutrient uptake. In: Bassiri Rad H (ed) Nutrient acquisition by plants: an ecological perspective. Springer, BerlinGoogle Scholar
  77. Randhir R, Shetty K (2005) Developmental stimulation of total phenolics and related antioxidant activity in light- and dark-germinated corn by natural elicitors. Process Biochem 40:1721–1732CrossRefGoogle Scholar
  78. Rao MSL, Kulkarni S, Lingaraaju S, Nadaf HL (2009) Bio priming of seeds: a potential tool in the integrated management of Alternaria blight of sunflower. Helia 32:107–114CrossRefGoogle Scholar
  79. Razmjoo K, Heydarizadeh P, Sabzalian MR (2008) Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomile. Int J Agric Biol 10:451–454Google Scholar
  80. Rengel Z, Graham RD (1995) Importance of seed Zn content for wheat growth on Zn deficient soil. II. Grain yield. Plant Soil 173:267–274CrossRefGoogle Scholar
  81. Roberts DR, Dumbroff EB, Thompson JE (1986) Exogenous polyamines alter membrane fluidity in bean leaves- a basis for their potential misinterpretation of their true physiological role. Planta 167(3):395–401PubMedCrossRefGoogle Scholar
  82. Ros C, Bell RW, White PF (1997) Effect of seed phosphorus and soil phosphorus applications on early growth of rice (Oryza sativa L) cv IR66. Soil Sci Plant Nutr 43:499–509CrossRefGoogle Scholar
  83. Roy NK, Srivastava AK (1999) Effect of presoaking seed treatment on germination and amylase activity of wheat under salt stress condition. Rachis 18:46–51Google Scholar
  84. Sallam HA (1999) Effect of some seed soaking treatments on growth and chemical components on faba bean plants under saline conditions. Ann Agric Sci 44:159–171Google Scholar
  85. Sandhya V, Ali SKZ, Grover M, Reddy G, Venkateswaralu B (2009) Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP – P45. Biol Fertil Soils 46:17–26CrossRefGoogle Scholar
  86. Saravanakumar D, Kavino M, Raguchander T, Subbiah P, Samiyappan R (2011) Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiol Plant 33:203–209CrossRefGoogle Scholar
  87. Scaife MA, Smith R (1973) The phosphorus requirement of lettuce. II. A dynamic model of phosphorus uptake and growth. J Agric Sci 80:353–361CrossRefGoogle Scholar
  88. Shafi M, Bakht J, Hassan MJ, Raziuddin M, Zhang G (2009) Effect of cadmium and salinity stresses on growth and antioxidant enzyme activities of wheat (Triticum aestivum L). Bull Environ Contam Toxicol 82:772–776PubMedCrossRefGoogle Scholar
  89. Sharma PC, Kumar P (1999) Alleviation of salinity stress during germination in Brassica juncea by pre sowing chilling treatments to seeds. Biol Plant 42:451–455CrossRefGoogle Scholar
  90. Shinozaki K, Shinozaki YK (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227PubMedCrossRefGoogle Scholar
  91. Soltani A, Gholipoor M, Zeinali E (2006) Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ Exp Bot 55:195–200CrossRefGoogle Scholar
  92. Sousa SF, Lopes AB, Fernandes PA, Ramos MJ (2009) The zinc proteome: a tale of stability and functionality. Dalton Trans 38:7946–7956PubMedCrossRefGoogle Scholar
  93. Souza-Machado V, Pitblado R, Ali A, May P (1999) Paclobutrazol in tomato for improved tolerance to early transplanting and early harvest maturity. Acta Hortic 487:139–144CrossRefGoogle Scholar
  94. Steponkus PL, Uemura M, Webb MS (1993) A contrast of the cryostability of the plasma membrane of winter rye and spring oat-two species that widely differ in their freezing tolerance and plasma membrane lipid composition. In: Steponkus PL (ed) Advances in low-temperature biology, vol 2. JAI Press, London, pp 211–312Google Scholar
  95. Sung FJM, Chang YH (1993) Biochemical activities associated with priming of sweet corn seeds to improve vigor. Seed Sci Technol 21:97–105Google Scholar
  96. Suzuki K, Nagasuga K, Okada M (2008) The chilling injury induced by high root temperature in the leaves of rice seedlings. Plant Cell Physiol 49:433–442PubMedCrossRefGoogle Scholar
  97. Tanaka Y, Hibino T, Hayashi Y, Tanaka A, Kishitani S, Takabe T, Yokota S, Takabe T (1999) Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts. Plant Sci 148:131–138CrossRefGoogle Scholar
  98. Terry N, Abadia J (1986) Function of iron in chloroplasts. J Plant Nutr 9:609–646CrossRefGoogle Scholar
  99. Thomson CJ, Bolger TP (1993) Effects of seed phosphorus concentration on the emergence and growth of subterranean clover (Trifolium subterraneum L). Plant and Soil 156:285–288CrossRefGoogle Scholar
  100. Timmusk S, Wagner EGH (1999) The plant growth promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses. Mol Plant-Microbes Interact 12:951–959CrossRefGoogle Scholar
  101. Timperio AM, D’Amici GM, Barta C, Loreto F, Zolla L (2007) Proteomics pigment composition and organization of thylakoid membranes in iron-deficiency spinach leaves. J Exp Bot 13:3695–3710CrossRefGoogle Scholar
  102. Vari A, Mitrabinda S, Dadlani M, Sharma SP (2003) Physiological and biochemical changes associated with osmopriming in maize seeds Paper presented in II International Congress of Plant Physiology, 8–12 January 2003, New Delhi p 113Google Scholar
  103. Varier A, Vari AK, Dadlani M (2010) The subcellular basis of seed priming. Curr Sci 99(4):45–456Google Scholar
  104. Venkateswarlu B, Desai S, Prasad YG (2008) Agriculturally important microorganisms for stressed ecosystems: challenges in technology development and application. In: Kachatou-rians GG, Arora DK, Rajendran TP, Srivastava AK (eds) Agriculturally important microorganisms, vol 1. Academic World, Bhopal, pp 225–246Google Scholar
  105. Verslues PE, Batelli G, Grillo S, Agius F, Kim YS, Zhu J, Agarwal M, Katiyar-Agarwal S, Zhu JK (2007) Interaction of SOS2 with nucleoside diphosphate kinase 2 and catalases reveals a point of connection between salt stress and H2O2 signaling in Arabidopsis thaliana. Mol Cell Biol 27:7771–7780PubMedPubMedCentralCrossRefGoogle Scholar
  106. Vidhyasekharan P, Muthamilan M (1995) Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis 79:782–786CrossRefGoogle Scholar
  107. Vijayaraghavan H (1999) Effect of seed treatment with PGR on bhendi grown under sodic soil condition. Madras Agric J 86:247–249Google Scholar
  108. Vikas Yadav P, Sujata B, Surasanna (2009) Halopriming imparts to tolerance to salt and PEG induced drought stress in sugarcane. Agric Ecosyst Environ 134:24–28CrossRefGoogle Scholar
  109. Vollenweider P, Günthardt-Goerg MS (2005) Diagnosis of abiotic and biotic stress factors using the visible symptoms in foliage. Environ Pollut 137:455–465PubMedCrossRefGoogle Scholar
  110. Wahid A, Gelani S, Ashraf M, Foolad M (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223CrossRefGoogle Scholar
  111. Wang FZ, Wang QB, Kwon SY, Kwak SS, Su WA (2005) Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. J Plant Physiol 162:465–472PubMedCrossRefGoogle Scholar
  112. Whalley RDB, Mckell CM, Green LR (1966) Seedling vigor and the early non-photo synthetic stage of seedling growth in grasses. Crop Sci 6:147–150CrossRefGoogle Scholar
  113. Xiong L, Zhu JK (2002) Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell Environ 25:131–139PubMedCrossRefGoogle Scholar
  114. Xu SC, Hu J, Li YP, Ma WG, Zheng YY, Zhu SJ (2011) Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine. Plant Growth Regul 63(3):279–290CrossRefGoogle Scholar
  115. Yadav SK (2010) Cold stress tolerance mechanisms in plants: a review. Agron Sustain Dev 30:515–527CrossRefGoogle Scholar
  116. Yeaoung YR, Wilson JR, Murray GA (1996) Germination performance and loss of LEA proteins during muskmelon seed priming. Seed Sci Technol 24:429–439Google Scholar
  117. Yeh YM, Chiu KY, Chen CL, Sung JM (2005) Partial vacuum extends the longevity of primed bitter gourd seeds by enhancing their anti-oxidative activities during storage. Sci Hortic 104:101–112CrossRefGoogle Scholar
  118. Yu Q, Osborne LD, Rengel Z (1999) Increased tolerance to Mn deficiency in transgenic tobacco over producing superoxide dismutase. Ann Bot 84:543–547CrossRefGoogle Scholar
  119. Zhang M, Nyborg M, Mcgill WB (1990) Phosphorus concentration in barley (Hordeum vulgare L) seed: influence on seedling growth and dry matter production. Plant Soil 122:79–83CrossRefGoogle Scholar
  120. Zhang Q, Rue K, Mueller J (2014) The effect of glycine betaine priming on seed germination of six turfgrass species under drought salinity or temperature stress. Hortic Sci 49:1454–1460Google Scholar
  121. Zinn KE, Tunc-Ozdemir M, Harper JF (2010) Temperature stress and plants sexual reproduction: uncovering the weakest links. J Exp Bot 61:1959–1968PubMedPubMedCentralCrossRefGoogle Scholar
  122. Zwiazek JJ, Blake TJ (1990) Effects of preconditioning on electrolyte leakage and lipid-composition in black spruce (Picea mariana) stressed with polyethylene-glycol. Physiol Plant 79:71–77CrossRefGoogle Scholar

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© Springer India 2016

Authors and Affiliations

  1. 1.Section of Seed Science and TechnologyICAR-Indian Institute of Horticultural ResearchBangaluruIndia

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