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Effects of Plant Growth Regulator, Auxin Polar Transport Inhibitors on Somatic Embryogenesis and CmSERK Gene Expression in Cattleya maxima (Lindl.)

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Somatic Embryogenesis in Ornamentals and Its Applications

Abstract

The somatic embryogenesis is commonly used for rapid propagation of species of interest hard to reproduce, like orchids. The induction of somatic embryogenesis requires the establishment of a peculiar genetic expression pattern in the presence of the suitable environmental conditions and a favorable hormonal background, which can be obtained providing synthetic plant growth regulators.

We tested several combinations of plant growth regulators in different illumination conditions on leaf explants of Cattleya maxima. The most efficient production of embryos was achieved with exogenous cytokinin (thidiazuron) in dark conditions at the cut end of the leaf. The expression of CmSERK gene was higher in the presence of cytokinins. The effects of the different treatments are discussed.

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Abbreviations

NPA:

N-1-Naphthylphthalamic acid

TIBA:

2,3,5-Triiodobenzoic acid

PGRs:

Plant growth regulators

PAT:

Polar auxin transport

SE:

Somatic embryogenesis

TDZ:

Thidiazuron [1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea]

2,4-D:

2,4-Dichlorophenoxyacetic acid

BA:

N6-Benzyladenine

NAA:

Naphthaleneacetic acid

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Acknowledgments

We thank Dr. Quintana-Ascencio (University of Central Florida) for advice on graphics and data presentation and Carlos Iñiguez-Armijos (Universidad Técnica Particular de Loja) for helpful training on the graphics editor Inkscape.

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Authors and Affiliations

  1. Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, San Cayetano alto s/n, Loja, Ecuador

    Augusta Yadira Cueva-Agila & Jhelisa Medina

  2. Dipartimento di Biologia e Biotecnologie, Università degli Studi di Pavia, Via Ferrata 9, 27100, Pavia, Italy

    Lorenzo Concia & Rino Cella

Authors
  1. Augusta Yadira Cueva-Agila
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  2. Jhelisa Medina
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  3. Lorenzo Concia
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  4. Rino Cella
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Corresponding author

Correspondence to Augusta Yadira Cueva-Agila .

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Editors and Affiliations

  1. Department of Botany, Hamdard University, New Delhi, Delhi, India

    A. Mujib

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Cueva-Agila, A.Y., Medina, J., Concia, L., Cella, R. (2016). Effects of Plant Growth Regulator, Auxin Polar Transport Inhibitors on Somatic Embryogenesis and CmSERK Gene Expression in Cattleya maxima (Lindl.). In: Mujib, A. (eds) Somatic Embryogenesis in Ornamentals and Its Applications. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2683-3_16

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