Abstract
Metagenomics provides the opportunity to uncover many unculturable extremophilic microorganisms, which represent majority of the planet’s biological diversity and are being utilized in industries to further furnish industrial process and products. This strategy has resulted in the isolation of novel biocatalysts and bioactive molecules. Here in this chapter, we will review various strategies for manipulating enzyme attributes like its activity, stability, inhibition, designing novel substrates, and substrate-specific binding. We have also recapitulated the idea of functional proteomics and systems biology approach in protein engineering.
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Acknowledgments
The authors acknowledge the support from SERB, Department of Science and Technology (DST), Government of India (DST Fast Track Grant. No. SR/FT/LS-31/2012), and University Grants Commission (UGC), New Delhi, India (Grant No. 42-457/2013(SR)). Mehak Baweja duly acknowledges the University Research Scholarship (URS) from M.D. University, Rohtak, India.
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Baweja, M., Singh, P.K., Shukla, P. (2016). Enzyme Technology, Functional Proteomics, and Systems Biology Toward Unraveling Molecular Basis for Functionality and Interactions in Biotechnological Processes. In: Shukla, P. (eds) Frontier Discoveries and Innovations in Interdisciplinary Microbiology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2610-9_13
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DOI: https://doi.org/10.1007/978-81-322-2610-9_13
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