Abstract
Hydrogen has been mooted as future fuel on the basis of its carbon neutrality, renewable nature, and highest energy density. In the recent times, its economical production has gained attention. The present chapter deals with a comprehensive insight on dark-fermentative biohydrogen production process. This process is less energy intensive and environmentally benign, and waste materials can be used as substrate. Biochemical insight on hydrogen production via dark fermentation exemplifies the complexity of the process. The maximum H2 yield of 4 mol H2 per mol of glucose has been observed when fermentation followed a solely acetate pathway. The potential H2-producing microorganisms are present in various natural and man-made habitats such as sewage sludge, anaerobically digested sludge, animal waste, compost, hot springs, oceanic sediments, and soil. There are many advantages of working with mixed consortia, viz., presence of different hydrolytic enzymes, better oxygen tolerance, etc. Various pretreatment processes have been explored to enrich H2-producing microbes. A detailed pretreatment processes, viz., chemical, physical, combined treatments, etc., creates a selection pressure, which could effectively alter the microbial dynamics of the mixed culture. Molecular techniques like polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), terminal restriction fragment length polymorphism (T-RFLP), ribosomal intergenic spacer analysis (RISA), quantitative PCR (qPCR), single-strand conformation polymorphism (SSCP), fluorescence in situ hybridization (FISH), and fluorescence-activated cell sorting (FACS) could be used for advanced and rapid microbial characterization. Use of cheap, renewable, and easily available raw materials could bring down the production cost of bioH2.
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References
Bothe H, Falkenberg B, Nolteernsting U (1974) Properties and functions of the pyruvate: ferredoxin oxidoreductase from the blue-green alga Anabaena cylindrica. Arch Microbiol 96:291–304. doi:10.1007/BF00590185
Castello E, Gracia Y, Santos C, Iglesias T, Paolino G, Wenzel J, Borzacconi L, Etchebehere C (2009) Feasibility of biohydrogen production from cheese whey using a UASB reactor: links between microbial community and reactor performance. Int J Hydrogen Energy 34:5674–5682. doi:10.1016/j.ijhydene.2009.05.060
Chan ASK, Parkin TB (2002) Evaluation of potential inhibitors of methanogenesis and methane oxidation in a landfill cover soil. Soil Biol Biochem 32:1581–1590
Chang JS, Lee KS, Lin PJ (2002) Biohydrogen production with fixed-bed bioreactors. Int J Hydrogen Energy 27:1167–1174. doi:10.1016/S0360-3199(02)00130-1
Chen CC, Lin CY, Lin MC (2002) Acid-base enrichment enhances anaerobic hydrogen production process. Appl Microbiol Biotechnol 58:224–228. doi:10.1007/s002530100814
Chen CC, Chuang YS, Lin CY, Lay CH, Sen B (2012) Thermophilic dark fermentation of untreated rice straw using mixed cultures for hydrogen production. Int J Hydrogen Energy 37:15540–15546. doi:10.1016/j.ijhydene.2012.01.036
Cheong DY, Hansen CL (2006) Bacterial stress enrichment enhances anaerobic hydrogen production in cattle manure sludge. Appl Microbiol Biotechnol 72:635–643. doi:10.1007/s00253-006-0313-x
Daesh G, Mortenson LE (1967) Sucrose catabolism in Clostridium pasteurianum and its relation to N2 fixation. J Bacteriol 96:346–351. http://jb.asm.org/cgi/content/long/96/2/346
Das D, Veziroglu TN (2008) Advances in biological hydrogen production processes. Int J Hydrogen Energy 33:6046–6057. doi:10.1016/j.ijhydene.2008.07.098
Duangmanee T, Padmasiri SI, Simmons JJ, Raskin L, Sung S (2007) Hydrogen production by anaerobic microbial communities exposed to repeated heat treatments. Water Environ Res 79:975–983. doi:10.2175/193864702784247224
Fan YT, Zhang YH, Zhang SF, Hou HW, Ren BZ (2006) Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost. Bioresour Technol 97:500–505. doi:10.1016/j.biortech.2005.02.049
Fang HH, Liu H (2002) Effect of pH on hydrogen production from glucose by a mixed culture. Bioresour Technol 82:87–93. doi:10.1016/S0960-8524(01)00110-9
Fang HHP, Liu H, Zhang T (2002a) Characterization of a hydrogen-producing granular sludge. Biotechnol Bioeng 78:44–52. doi:10.1002/bit.10174
Fang HHP, Zhang T, Liu H (2002b) Microbial diversity of a mesophilic hydrogen producing sludge. Appl Microbiol Biotechnol 58:112–118. doi:10.1007/s00253-001-0865-8
Giovannoni SJ, DeLong EF, Olsen GJ, Pace NR (1988) Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. J Bacteriol 170:720–726. http://jb.asm.org/cgi/content/long/170/2/720
Han SK, Shin HS (2004) Biohydrogen production by anaerobic fermentation of food waste. Int J Hydrogen Energy 29:569–577. doi:10.1016/j.ijhydene.2003.09.001
Hawkes FR, Dinsdale R, Hawkes DL, Hussy I (2002) Sustainable fermentative hydrogen production: challenges for process optimisation. Int J Hydrogen Energy 27:1339–1347. doi:10.1016/S0360-3199(02)00090-3
Hawkes FR, Hussy I, Kyazze G, Dinsdale R, Hawkes DL (2007) Continuous dark fermentative hydrogen production by mesophilic microflora: principles and progress. Int J Hydrogen Energy 32:172–184. doi:10.1016/j.ijhydene.2006.08.014
Iyer P, Bruns MA, Husen Z, Van Ginkel S, Logan BE (2004) H2 producing bacterial communities from heat-treated soil inoculums. Appl Microbiol Biotechnol 66:166–173. doi:10.1007/s00253-004-1666-7
José LS, Thorsten K (2007) Molecular biology techniques used in wastewater treatment: an overview. Process Biochem 42:119–133. doi:10.1016/j.procbio.2006.10.003
Jungermann K, Thauer RK, Leimenstoll G, Decker K (1973) Function of reduced pyridine nucleotide-ferredoxin oxidoreductases in saccharolytic Clostridia. Biochim Biophys Acta 305:268–280. doi:10.1016/0005-2728(73)90175-8
Kalia VC, Jain SR, Kumar A, Joshi AP (1994) Fermentation of biowaste to H2 by Bacillus licheniformis. World J Microbiol Biotechnol 10:224–227. doi:10.1007/BF00360893
Kenealy W, Zeikus JG (1981) Influence of corrinoid antagonists on methanogen metabolism. J Bacteriol 146:133–140. http://jb.asm.org/cgi/content/long/146/1/133
Kim J, Park C, Kim TH, Lee M, Kim S, Kim S, Seung-Wook Lee J (2003) Effects of various pretreatments for enhanced anaerobic digestion with waste activated sludge. J Biosci Bioeng 95:271–275. doi:10.1016/S1389-1723(03)80028-2
Knappe J, Blaschkowski HP, Grobner P, Schmitt T (1974) Pyruvate formate lyase of Escherichia coli: the acetyl-enzyme intermediate. Eur J Biochem 50:253–263. doi:10.1111/j.1432-1033.1974.tb03894.x
Kotay SM (2008) Microbial hydrogen production from sewage sludge. PhD thesis, IIT Kharagpur
Kraemer JT, Bagley DM (2007) Improving the yield from fermentative hydrogen production. Biotechnol Lett 29:685–695. doi:10.1007/s10529-006-9299-9
Kumar P, Pant DC, Mehariya S, Sharma R, Kansal A, Kalia VC (2014) Ecobiotechnological strategy to enhance efficiency of bioconversion of wastes into hydrogen and methane. Indian J Microbiol 54:262–267. doi:10.1007/s12088-014-0467-7
Lay JJ, Fan KS, Chang J, Ku CH (2004) Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge. Int J Hydrogen Energy 28:1361–1367. doi:10.1016/S0360-3199(03)00027-2
Li C, Fang HH (2007) Fermentative hydrogen production from wastewater and solid wastes by mixed cultures. Crit Rev Environ Sci Technol 37:1–39. doi:10.1080/10643380600729071
Li RY, Zhang T, Fang HHP (2011) Application of molecular techniques on heterotrophic hydrogen production research. Bioresour Technol 102:8445–8456. doi:10.1016/j.biortech.2011.02.072
Lin CY, Lay CH (2005) A nutrient formulation for fermentative hydrogen production using anaerobic sewage sludge microflora. Int J Hydrogen Energy 30:285–292. doi:10.1016/j.ijhydene.2004.03.002
Liu WT, Marsh TL, Cheng H, Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of gene encoding 16S rRNA. Appl Environ Microbiol 63:4516–4522. http://aem.asm.org/cgi/content/long/63/11/4516
Loy A, Horn M, Wagner Z (2003) ProbeBase: an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res 31:514–516. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=165463&tool=pmcentrez&rendertype=abstract
Luo Y, Zhang H, Salerno M, Logan B, Bruns MA (2008) Organic loading rates affect composition of soil-derived bacterial communities during continuous, fermentative biohydrogen production. Int J Hydrogen Energy 33:6566–6576. doi:10.1016/j.ijhydene.2008.08.047
Mohan VS, Babu LV, Sarma PN (2008) Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate. Bioresour Technol 99:59–67. doi:10.1016/j.biortech.2006.12.004
Mu Y, Yu HQ, Wang G (2007) Evaluation of three methods for enriching hydrogen producing cultures from anaerobic sludge. Enzym Microb Technol 40:947–953. doi:10.1016/j.enzmictec.2006.07.033
Nandi R, Sengupta S (1996) Involvement of anaerobic reductase in the spontaneous lysis of formate by immobilized cells of E. coli. Enzym Microb Technol 19:20–25. doi:10.1016/0141-0229(95)00176-X
Nandi R, Sengupta S (1998) Microbial production of hydrogen: an overview. Crit Rev Microbiol 24:61–84. doi:10.1080/10408419891294181
Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechte M, Umbarger HE (1987) Escherichia coli and Salmonella typhimurium. In: Cellular and molecular biology, vols 1 & 2. American Society for Microbiology, Washington, DC
Noike T, Mizuno O (2000) Hydrogen fermentation of organic municipal wastes. Water Sci Technol 42:155–162. http://www.iwaponline.com/wst/04212/wst042120155.htm
Ohnishi A, Abe S, Bando Y, Fujimoto N, Suzuki M (2012) Rapid detection and quantification methodology for genus Megasphaera as a hydrogen producer in a hydrogen fermentation system. Int J Hydrogen Energy 37:2239–2247. doi:10.1016/j.ijhydene.2011.10.094
Oremland RS (1988) Biogeochemistry of methanogenic bacteria. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York
Orhon D, Gorgun E, Germirli F, Artan N (1993) Biological treatability of dairy wastewaters. Water Res 27:625–633. doi:10.1016/0043-1354(93)90172-E
O-Thong S, Prasertsan P, Karakashev D, Angelidaki I (2008) 16S rRNA-targeted probes for specific detection of Thermoanaerobacterium sp., Thermoanaerobacterium thermosaccharolyticum, and Caldicellulosiruptor sp, by fluorescent in situ hybridization in biohydrogen production systems. Int J Hydrogen Energy 33:6082–6091. doi:10.1016/j.ijhydene.2008.07.094
O-Thong S, Prasertsan P, Kare Birkeland N (2009) Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. Bioresour Technol 100:909–918. doi:10.1016/j.biortech.2008.07.036
O-Thong S, Mamimin C, Prasertsan P (2011) Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis. Electron J Biotechnol 14:1–12. doi:10.2225/vol14-issue5-fulltext-9
Patel SKS, Purohit HJ, Kalia VC (2010) Dark fermentative hydrogen production by defined mixed microbial cultures immobilized on ligno-cellulosic waste materials. Int J Hydrogen Energy 35:10674–10681. doi:10.1016/j.ijhydene.2010.03.025
Quemeneur M, Hamelin J, Latrille E, Steyer JP, Trably E (2010) Development and application of a functional CE-SSCP fingerprinting method based on Fe-Fe hydrogenase genes for monitoring hydrogen – producing Clostridium in mixed cultures. Int J Hydrogen Energy 35:13158–13167. doi:10.1016/j.ijhydene.2010.07.076
Roy S, Ghosh S, Das D (2012) Improvement of hydrogen production with thermophilic mixed culture using rice spent wash of distillery industry. Int J Hydrogen Energy 37:15867–15874. doi:10.1016/j.ijhydene.2012.08.016
Sá LRV, de Oliveira TC, dos Santos TF, Matos A, Cammarota MC, Oliveira EMM et al (2011) Hydrogenase activity monitoring in the fermentative hydrogen production using heat pretreated sludge: a useful approach to evaluate bacterial communities performance. Int J Hydrogen Energy 36:7543–7549. doi:10.1016/j.ijhydene.2011.03.119
Solomon BO, Zeng AR, Biebl H, Schlieker H, Posten C, Deckwer WD (1995) Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol. J Biotechnol 39:107–117. doi:10.1016/0168-1656(94)00148-6
Sparling R, Risbey D, Poggi-Varaldo HM (1997) Hydrogen production from inhibited anaerobic composters. Int J Hydrogen Energy 22:563–566. doi:10.1016/S0360-3199(96)00137-1
Sprott GD, Jarrell KF, Shaw KM, Knowles R (1982) Acetylene as an inhibitor of methanogenic bacteria. J Gen Microbiol 128:2453–2462. doi:10.1099/00221287-128-10-2453
Thauer RK, Jungermann K, Decker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41:100–180. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=413997&tool=pmcentrez&rendertype=abstract
Tolvanen KES, Karp MT (2011) Molecular methods for characterizing mixed microbial communities in hydrogen-fermenting systems. Int J Hydrogen Energy 36:5280–5288. doi:10.1016/j.ijhydene.2011.01.029
Ueno Y, Kawai T, Sato S, Otsuka S, Morimoto M (1995) Biological production of hydrogen from cellulose by natural anaerobic microflora. J Ferment Bioeng 79:395–397. doi:10.1016/0922-338X(95)94005-C
Ueno Y, Otsuka S, Morimoto M (1996) Hydrogen production from industrial wastewater by anaerobic microflora in chemostat culture. J Ferment Bioeng 2:194–197. doi:10.1016/0922-338X(96)85050-1
Uyeda K, Rabinowitz JR (1971) Pyruvate-ferredoxin oxidoreductase. IV. Studies on the reaction mechanism. J Biol Chem 246:3120–3125. http://www.jbc.org/cgi/content/long/246/10/3120
Valdez-Vazquez I, Rios-Leal E, Esparza-Garcia F, Cecchi F, Poggi-Varaldo HM (2005a) Semi-continuous solid substrate anaerobic reactors for H2 production from organic waste: mesophilic versus thermophilic regime. Int J Hydrogen Energy 30:1383–1391. doi:10.1016/j.ijhydene.2004.09.016
Valdez-Vazquez I, Sparling R, Rinderknecht-Seijas N, Risbey D, Poggi-Varaldo HM (2005b) Hydrogen from the anaerobic fermentation of industrial solid waste. Bioresour Technol 96:1907–1913. doi:10.1016/j.biortech.2005.01.036
Venkata Mohan S, Mohanakrishna G, Ramanaiah SV, Sarma PN (2008) Simultaneous biohydrogen production and wastewater treatment in biofilm configured anaerobic periodic discontinuous batch reactor using distillery wastewater. Int J Hydrogen Energy 33:550–558. doi:10.1016/j.ijhydene.2007.10.013
Venkata Mohan S, Veer Raghuvulu S, Mohanakrishna G, Srikanth S, Sarma PN (2009) Optimization and evaluation of fermentative hydrogen production and wastewater treatment processes using data enveloping analysis (DEA) and Taguchi design of experimental (DOE) methodology. Int J Hydrogen Energy 34:216–226. doi:10.1016/j.ijhydene.2008.09.044
von Wintzingerode F, Gobel UB, Stackebrandt E (1997) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiol Rev 21:213–229. doi:10.1111/j.1574-6976.1997.tb00351.x
Wang CC, Chang CW, Chu CP, Lee DJ, Chang BV, Liao CS, Tay JH (2003a) Using filtrate of waste biosolids to effectively produce bio-hydrogen by anaerobic fermentation. Water Res 37:2789–2793. doi:10.1016/S0043-1354(03)00004-6
Wang CC, Chang C, Chu CP, Lee DJ, Chang BV, Liao CS (2003b) Producing hydrogen from wastewater sludge by Clostridium bifermentans. J Biotechnol 102:83–92. doi:10.1016/S0168-1656(03)00007-5
Wanger M, Horn M, Daims H (2003) Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr Opin Microbiol 6:302–309. doi:10.1016/S1369-5274(03)00054-7
Watanabe H, Kitamura T, Ochi S, Ozaki M (1997) Inactivation of pathogenic bacteria under mesophilic and thermophilic conditions. Water Sci Technol 36:25–32. doi:10.1016/S0273-1223(97)00503-9
Woese CR (1987) Bacterial evolution. Microbiol Rev 51:221–271. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=373105&tool=pmcentrez&rendertype=abstract
Yu H, Zhu Z, Hu W, Zhang H (2002) Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures. Int J Hydrogen Energy 27:1359–1365. doi:10.1016/S0360-3199(02)00073-3
Zhang T, Fang HHP (2006) Application of real-time polymerase chain reaction for quantification of microorganisms in environmental samples. Appl Microbiol Biotechnol 70:281–289. doi:10.1007/s00253-006-0333-6
Zhu H, Beland M (2006) Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge. Int J Hydrogen Energy 31:1980–1988. doi:10.1016/j.ijhydene.2006.01.019
Acknowledgments
Authors are thankful to the Council for Scientific and Industrial Research (CSIR), Department of Biotechnology (DBT), Ministry of New and Renewable Energy (MNRE), Defence Research and Development Organisation (DRDO), and Department of Science and Technology (DST), Government of India, for the financial assistance.
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Roy, S., Das, D. (2015). Ecobiotechnological Approaches: Enrichment Strategy for Improvement of H2 Production. In: Kalia, V. (eds) Microbial Factories. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2598-0_3
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