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Phylogenetic Affiliation of Pseudomonas sp. MO2, a Novel Polyhydroxyalkanoate-Synthesizing Bacterium

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Microbial Factories

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Abstract

A bacterium, isolated from wastewater after enrichment with waste canola fryer oil, was found to synthesize 12–20 % of cell dry weight (cdw) medium chain length polyhydroxyalkanoates (mcl-PHAs) using different carbon substrates. On the basis of partial 16S rDNA sequence analysis, this bacterium was first identified as Pseudomonas putida and designated as P. putida strain MO2. However, the full 16S rDNA gene sequence from a whole genome sequence analysis of this strain showed 100 % identity to 16S rDNA of Pseudomonas monteilii SB3101 and Pseudomonas monteilii SB3078. The comparison of the cpn60 gene sequence of Pseudomonas sp. strain MO2 with strains of P. putida, P. aeruginosa, P. fluorescens, P. stutzeri, P. syringae, and P. monteilii indicated that this strain is more closely related to P. monteilii than P. putida type strain KT2440. Based on gene sequence similarity index and phylogenetic analyses, some P. putida strains, which were earlier classified as P. putida, are also more closely related to the P. monteilii cluster. Our analyses show that P. putida is a very diverse group with divergent strains, and many strains of P. putida that cluster with P. monteilii species may need to be reclassified.

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Acknowledgments

This work was supported by funds provided by the Natural Sciences and Engineering Research Council of Canada (NSERC), through a Strategic Programs grant (STPGP 306944-04), by Genome Canada, through the Applied Genomics Research in Bioproducts or Crops (ABC) program for the grant titled “Microbial Genomics for Biofuels and CoProducts from Biorefining Processes,” and by the Province of Manitoba, through the Manitoba Research Innovation Fund (MRIF).

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Authors and Affiliations

  1. Department of Biosystems Engineering, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada

    Parveen Kumar Sharma, Jilagamazhi Fu & David B. Levin

  2. Department of Plant Science, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada

    Xiang Zhang

  3. Department of Microbiology, University of Manitoba, R3T 2N2, Winnipeg, Manitoba, Canada

    Richard Sparling

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  1. Parveen Kumar Sharma
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  2. Jilagamazhi Fu
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  3. Xiang Zhang
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  4. Richard Sparling
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  5. David B. Levin
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Correspondence to David B. Levin .

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  1. CSIR, Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi, India

    Vipin Chandra Kalia

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Sharma, P.K., Fu, J., Zhang, X., Sparling, R., Levin, D.B. (2015). Phylogenetic Affiliation of Pseudomonas sp. MO2, a Novel Polyhydroxyalkanoate-Synthesizing Bacterium. In: Kalia, V. (eds) Microbial Factories. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2595-9_4

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