Skip to main content
SpringerLink
Log in

Combined Approach of Morphological and Molecular Diagnosis of Fusaria spp. Causing Diseases in Crop Plants

  • Chapter
Recent Advances in the Diagnosis and Management of Plant Diseases

Abstract

Fusarium species cause a huge range of diseases on an extraordinary range of host plants. Fusarium taxonomy has been plagued by changing species concepts, with as few as nine or well over 1,000 species being recognized by various taxonomists during the past 100 years, depending on the species concept employed. The possible outcome from morphological diagnosis alone may identify quickly when the number of species associated with particular host or disease symptoms is relatively limited. Morphological characters frequently are homoplastic, and the circumscription of taxa, based on the size and shape of conidia and conidiophores and the color and texture of colonies, has resulted in an underestimation of species diversity within Fusarium. Phylogenetic species recognition, based on DNA sequence data from multiple loci, allows greater numbers of species to be distinguished than in the exclusive use of morphological features. Closely related species may show considerable divergence in IGS, often reflecting both length and sequence variation; length and restriction site variation may even occur within the rDNA of an individual in some fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Appel DJ, Gordon TR (1995) Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Exp Mycol 19:120–128

    Article  CAS  PubMed  Google Scholar 

  • Backhouse D, Burgess LW (2002) Climatic analysis of the distribution of Fusarium graminearum, F. pseudograminearum and F. culmorum on cereals in Australia. Australas Plant Pathol 31:321–327

    Article  Google Scholar 

  • Backhouse D, Burgess LW, Summerell BA (2001) Biogeography of Fusarium. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW (eds) Fusarium: Paul E. Nelson memorial symposium. American Phytopathological Society, St. Paul, pp 124–139

    Google Scholar 

  • Bulat SA, Alekhina IA, Kozlova ED (1997) Molecular taxonomy of Fusarium fungi by means of ribotyping rDNA sequencing and UP–PCR analysis. A case study of taxa in Sporotrichiella section. Cereal Res Commun 25:565–570

    CAS  Google Scholar 

  • Burgess LW, Summerell BA (1992) Mycogeography of Fusarium: survey of Fusarium species in subtropical and semi-arid grasslands soils in Queensland. Mycol Res 96:780–784

    Article  Google Scholar 

  • Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D (1994) Laboratoly manual for Fusarium research, 3rd edn. The University of Sydney Royal Botanic Gardens, Sydney

    Google Scholar 

  • Chesters CGC, Thornton RH (1956) A comparison of techniques for isolating soil fungi. Trans Br Mycol Soc 39:301

    Article  Google Scholar 

  • El-Fadly GB, El-Kazzaz MK, Hassan MAA, El-Kot GAN (2008) Identification of some Fusarium spp. using RAPD-PCR technique. Egypt J Phytopathol 36(1–2):71–80

    Google Scholar 

  • Fisher NL, Burgess LW, Toussoun TA, Nelson PE (1982) Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72:151–153

    Article  Google Scholar 

  • Garrett SD (1981) Soil fungi and soil fertility, 2nd edn. Pergamon Press, Oxford

    Google Scholar 

  • Geiser DM, Jimenez Gasco MM, Kang S, Mkalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K (2004) FUSARIUM-IDv.1.0: a DNA sequence database for identifying Fusarium. Eur J Plant Pathol 110:473–479

    Article  CAS  Google Scholar 

  • Gonzalez Jaén MT, Mirete S, Patiño B, López-Errasquín E, Vázquez C (2004) Genetic markers for the análysis of variability and for production of specific diagnostic sequences in fumonisin-producing strains of Fusarium verticillioides. Eur J Plant Pathol 110:525–532

    Article  Google Scholar 

  • Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 66:411–453

    Article  CAS  PubMed  Google Scholar 

  • Jasalavich CA, Ostrofsky A, Jellison J (2000) Detection and identification of decay fungi in spruce wood by Restriction Fragment Length Polymorphism analysis of amplified genes encoding rRNA. Appl Environ Microbiol 66:4725–4734

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Jurado M, Vázquez C, López-Errasquin E, Patiño B, González-Jaén MT (2004) Analysis of the occurrence of Fusarium species in Spanish cereals by PCR assays. In: Proceedings of the 2nd international symposium on Fusarium head blight and 8th European Fusarium seminar, vol 2, pp 460–464

    Google Scholar 

  • Jurado M, Vázquez C, Callejas C, González-Jaén MT (2006a) Occurrence and variability of mycotoxigenic Fusarium species associated to wheat and maize in the South West of Spain. Mycotoxin Res 22:87–91

    Article  CAS  PubMed  Google Scholar 

  • Jurado M, Vázquez C, Marín S, Sanchis V, González-Jaén MT (2006b) PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize. Syst Appl Microbiol 29:681–689

    Article  CAS  PubMed  Google Scholar 

  • Komada H (1975) Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Rev Plant Prot Res 8:114–125

    Google Scholar 

  • Konstantinovaa P, Yli-Mattila T (2004) IGS–RFLP analysis and development of molecular markers for identification of Fusarium poae, Fusarium langsethiae, Fusarium sporotrichioides and Fusarium kyushuense. Int J Food Microbiol 95(3):321–331

    Article  Google Scholar 

  • Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Blackwell Publishing, Ames, p 388

    Book  Google Scholar 

  • Leslie JF, Zeller KA, Summerell BA (2001) Icebergs and species in populations of Fusarium. Physiol Mol Plant Pathol 59:107–117

    Article  Google Scholar 

  • Martin FN (1990) Variation in the ribosomal DNA repeat unit within single-oospore isolates of the genus Pythium. Genome 33:585–591

    Article  CAS  Google Scholar 

  • McMullen MP, Stack RW (1983) Effects of isolation techniques and media on the differential isolation of Fusarium species. Phytopathology 73:458–462

    Article  Google Scholar 

  • Mulé G, González Jaén MT, Hornok L, Nicholson P, Waalwijk C (2005) Advances in molecular diagnosis of toxigenic Fusarium species: a review. Food Addit Contam 22:316–323

    Article  PubMed  Google Scholar 

  • Nelson PE, Toussoun TA, Cook RJ (eds) (1981) Fusarium: diseases, biology and taxonomy. Pennsylvania State University, University Park

    Google Scholar 

  • Nitschke E, Nihlgard M, Varrelmann M (2009) Differentiation of eleven Fusarium spp. Isolated from sugar beet, using restriction fragment analysis of a polymerase chain reaction–amplified translation elongation factor 1α gene fragment. Mycology 99(8):921–929

    CAS  Google Scholar 

  • O’Donnell K (1996) Progress towards a phylogenetic classification of Fusarium. Sydowia 48:57–70

    Google Scholar 

  • Paavanen-Huhtala S (2000) Molecular-based assays for the determination of diversity and identification of Fusarium and Gliocladium fungi. PhD thesis, Annales Universitatis Turkvensis AII 139, University of Turku, Finland, pp 198

    Google Scholar 

  • Paavanen-Huhtala S, Hyvonen J, Bulat SA, Yli-Mattila T (1999) RAPD–PCR, isozyme, rDNA RFLP and rDNA sequence analyses in identification of Finnish Fusarium oxysporum isolates. Mycol Res 103:625–634

    Article  CAS  Google Scholar 

  • Ploetz RC (2001) Malformation: a unique and important disease of mango, Magnifera indica L. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW (eds) Fusarium: Paul E. Nelson memorial symposium. American Phytopathological Society, St. Paul, pp 233–247

    Google Scholar 

  • Rahjoo V, Zad J, Javan-Nikkhah M, Mirzadi Gohari A, Okhovvat SM, Bihamta MR, Razzaghian J, Klemsdal SS (2008) Morphological and molecular identification of Fusarium isolated from maize ears in Iran. J Plant Pathol 90:463–468

    CAS  Google Scholar 

  • Reinking OA, Wollenweber HW (1927) “Tropical Fusaria” in Philippine. J Sci 32(2):103–252

    Google Scholar 

  • Sarlin T, Yli-Mattila T, Jestoi M, Rizzo A, Paavanen-Huhtala S, Haikara A (2006) Real-time PCR for quantification of toxigenic Fusarium species in Barley and Malt. Eur J Plant Pathol 114:371–380

    Article  CAS  Google Scholar 

  • Sreenivasa MY, González-Jaén MT, Dass RS, Rharith Raj AP, Janardhana GR (2008) A PCR-based assay for the detection and differentiation of potential fumonisin-producing Fusarium verticillioides isolated from Indian maize Kernels. Food Biotechnol 22:160–170

    Article  CAS  Google Scholar 

  • Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW (eds) (2001) Fusarium: Paul E. Nelson memorial symposium. American Phytopathological Society, St. Paul

    Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 31:21–32

    Article  CAS  PubMed  Google Scholar 

  • Tio M, Burgess LW, Nelson PE, Toussoun TA (1977) Techniques for the isolation, culture and preservation of the Fusaria. Aust Plant Pathol Soc Newslett 6:11–13

    Article  Google Scholar 

  • Warcup JH (1950) The soil-plate method for isolation of fungi from soil. Nature (London) 146:117

    Article  Google Scholar 

  • Warcup JH (1955) On the origin of colonies of fungi developing on soil-dilution plates. Trans Br Mycol Soc 38:298–301

    Article  Google Scholar 

  • Wensly RN, McKeen CD (1962) Rapid test for pathogenicity of soil isolates of Fusarium oxysporum of sp. melonis. Can J Microbiol 8:818–819

    Article  Google Scholar 

  • Yli-Mattila T, Paavanen-Huhtala S, Bulat SA, Alekhina IA, Nirenberg HI (2002) Molecular, morphological and phylogenetic analysis of the Fusarium avenaceum/F. arthrosporioides/F. tricinctum species complex—a polyphasic approach. Mycol Res 106:655–669

    Article  CAS  Google Scholar 

  • Young-Mi L, Choi Y, Min B (2000) PCR-RFLP and sequence analysis of the rDNA ITS region in the Fusarium spp. J Microbiol 38(2):66–73

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Prameeladevi Thokala, Deeba Kamil, Pankaj Pandey, Prabhakaran Narayanasamy & Nita Mathur

Authors
  1. Prameeladevi Thokala
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Deeba Kamil
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pankaj Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Prabhakaran Narayanasamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nita Mathur
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Prameeladevi Thokala .

Editor information

Editors and Affiliations

  1. Department of Plant Pathology, N.D. University of Agriculture and Technology, Kumarganj, Faizabad, Uttar Pradesh, India

    L. P. Awasthi

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer India

About this chapter

Cite this chapter

Thokala, P., Kamil, D., Pandey, P., Narayanasamy, P., Mathur, N. (2015). Combined Approach of Morphological and Molecular Diagnosis of Fusaria spp. Causing Diseases in Crop Plants. In: Awasthi, L.P. (eds) Recent Advances in the Diagnosis and Management of Plant Diseases. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2571-3_3

Download citation

Publish with us

Policies and ethics

Search

Navigation