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Molecular Tools and Techniques for Detection and Diagnosis of Plant Pathogens

  • Pramila Pandey
  • N. S. Pandey
  • Md. Shamim
  • Deepti Srivastava
  • D. K. Dwivedi
  • L. P. Awasthi
  • K. N. Singh

Abstract

Plant pathogens infect a wide range of plant species and cause great yield and quality loss of agricultural crops. Detection and accurate identification of harmful plant pathogens is very essential to improve the strategies for controlling plant diseases. The early detection and identification of plant pathogens provides the basis for understanding their biology and appropriate strategies to control that particular pathogen. For the identification of plant pathogen, traditional procedures, i.e., isolation, in vitro culturing and microscopy of the extracellular pathogens, are in common routine. However, traditional methods may take days or weeks for particular pathogens to produce diagnostic spores. Indexing for many intracellular pathogens is also very complex because they are obligate biotrophs in nature. The development in the recent tools in molecular biology has enhanced and accelerated the detection and diagnostics through the automatic purification of nucleic acids and specific proteins from pathogens.

Keywords

Polymerase Chain Reaction Plant Pathogen Cucumber Mosaic Virus Multiplex Polymerase Chain Reaction Nest Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer India 2015

Authors and Affiliations

  • Pramila Pandey
    • 1
  • N. S. Pandey
    • 2
  • Md. Shamim
    • 3
    • 4
  • Deepti Srivastava
    • 1
  • D. K. Dwivedi
    • 1
  • L. P. Awasthi
    • 4
  • K. N. Singh
    • 1
  1. 1.Department of Plant Molecular Biology and Genetic EngineeringN.D. University of Agriculture and TechnologyFaizabadIndia
  2. 2.Department of BotanyNational P.G. CollegeLucknowIndia
  3. 3.Department of Molecular Biology and Genetic EngineeringBihar Agricultural UniversitySabour BhagalpurIndia
  4. 4.Department of Plant PathologyN.D. University of Agriculture and TechnologyFaizabadIndia

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