Novel Detection Techniques for Plant Pathogens and Their Application in Disease Management

  • Sandeep Jain
  • T. S. Thind
  • P. S. Sekhon
  • Amarjit Singh


Precise disease diagnosis and pathogen detection are of utmost importance, as without this ability we can neither hope to understand the disease nor in many cases control it. Detection deals with establishing the presence of a particular target organism within a sample, including symptomless individuals. Diagnosis relates to the identification of the nature and cause of the disease problem and thus deals with plants showing the symptoms. All aspects of plant disease epidemiology, from disease spread to estimation of yield losses, require the ability to identify the pathogen. Implementation of plant disease regulations through quarantine also requires the ability to diagnose plant disease. Increasing globalization and the international trade in plants and plant products also poses threat of inadvertent introduction of exotic pests and pathogens. Hence, importance of diagnostic tests has increased manyfold in modern era. In addition to detecting new invasive species, rapid and accurate diagnostic tests are required to monitor the emergence of novel variants of well-established pathogens. Improved techniques are vital to safeguard food security in the face of threatening diseases emerging as a consequence of climate change or other environmental shifts, or due to new agricultural practices. Detection of pathogens may be carried out either by employing conventional or relatively modern methods.


Polymerase Chain Reaction Multiplex Polymerase Chain Reaction Polymerase Chain Reaction Detection Conventional Polymerase Chain Reaction Electron Microscope Grid 
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  1. Ahoonmanesh A, Hajimorad MR, Ingham BJ, Francki RIB (1990) Indirect double antibody sandwich ELISA for detecting alfalfa mosaic virus in aphids after short probes on infected plants. J Virol Methods 30:27l–282lCrossRefGoogle Scholar
  2. Cahill DM, Hardham AR (1994) Exploitation of zoospore taxis in the development of a novel dipstick immunoassay for the specific detection of Phytophthora cinnamomi. Phytopathology 84:193–200CrossRefGoogle Scholar
  3. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme linked immuno-sorbent assay for the detection of plant viruses. J Gen Virol 34:475–483CrossRefPubMedGoogle Scholar
  4. Danks C, Barker I (2000) On-site detection of plant pathogens using lateral-flow devices. EPPO Bull 30:421–426CrossRefGoogle Scholar
  5. Fraaije BA, Butters JA, Coelho JM, Jones DR, Hollomon DW (2002) Following the dynamics of strobilurin resistance in Blumeria graminis f.sp. tritici using quantitative allele-specific real-time PCR measurements with the fluorescent dye SYBR Green I. Plant Pathol 51:45–54CrossRefGoogle Scholar
  6. Gwinn KD, Collins-Shephard MH, Reddick BB (1991) Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology 81:747–748CrossRefGoogle Scholar
  7. Harmon PF, Dunkle LD, Latin R (2003) A rapid PCR-based method for the detection of Magnaporthe oryzae from infected perennial ryegrass. Plant Dis 87:1072–1076CrossRefGoogle Scholar
  8. Kennedy R, Wakeham AJ, Byrne KG, Meyer UM, Dewey FM (2000) A new method to monitor airborne inoculum of the fungal plant pathogens Mycosphaerella brassicicola and Botrytis cinerea. Appl Environ Microbiol 66(7):2996–3003PubMedCentralCrossRefPubMedGoogle Scholar
  9. Koenig R (1978) ELISA in the study of homologous and heterologous reactions of plant viruses. J Gen Virol 40:309–318CrossRefGoogle Scholar
  10. Miller SA, Bhat RG, Schmitthenner AF (1994) Detection of Phytophthora capsici in pepper and cucurbit crops in Ohio with two commercial immunoassay kits. Plant Dis 78:1042–1046CrossRefGoogle Scholar
  11. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63PubMedCentralCrossRefPubMedGoogle Scholar
  12. Papavizas GC, Bowers JH, Johnston SA (1981) Selective isolation of Phytophthora capsici from soils. Phytopathology 71:129–133CrossRefGoogle Scholar
  13. Paterson RM (2006) Identification and quantification of mycotoxigenic fungi by PCR. Process Biochem 41:1467–1474CrossRefGoogle Scholar
  14. Schaad NW, Berthier-Schaad Y, Sechler A, Knorr D (1999) Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system. Plant Dis 83:1095–1100CrossRefGoogle Scholar
  15. Schaad NW, Frederick RD, Shaw J, Schneider WL, Hickson R, Petrillo M, Luster DG (2003) Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. Annu Rev Phytopathol 41:305–324CrossRefPubMedGoogle Scholar
  16. Shine JM, Comstock JC (1993) Digital image analysis system for determining tissue-blot immunoassay results for ratoon stunting disease of sugarcane. Plant Dis 77:511–513CrossRefGoogle Scholar
  17. Walcot RR (2003) Detection of seedborne pathogens. Hortic Technol 13(1):40–47Google Scholar
  18. Ward E, Foster SJ, Fraaije BA, Mccartney HA (2004) Plant pathogen diagnostics: immunological and nucleic acid-based approaches. Ann Appl Biol 145:1–16CrossRefGoogle Scholar
  19. Ward L, Harper S, Clover G (2010) Development of a LAMP assay for Xylella fastidiosa. MAF Biosecurity New Zealand technical paper No: 2010/14Google Scholar
  20. Waterhouse PM, Chu PWG (1995) Nucleic acid based approaches to plant virus and viroid diagnostics. In: Skerritt JH, Appels R (eds) New diagnostics in crop sciences, Biotechnology in agriculture No. 13. CAB International, Wallingford, UK, pp 195–214Google Scholar
  21. Zhang ZG, Li YQ, Fan H, Wang YC, Zheng XB (2006) Molecular detection of Phytophthora capsici in infected plant tissues, soil and water. Plant Pathol 55:770–775CrossRefGoogle Scholar

Copyright information

© Springer India 2015

Authors and Affiliations

  • Sandeep Jain
    • 1
  • T. S. Thind
    • 1
  • P. S. Sekhon
    • 1
  • Amarjit Singh
    • 1
  1. 1.Department of Plant PathologyPunjab Agricultural UniversityLudhianaIndia

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