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Chitosan: A Promising Substrate for Regenerative Medicine in Drug Formulation

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Chitin and Chitosan for Regenerative Medicine

Part of the book series: Springer Series on Polymer and Composite Materials ((SSPCM))

Abstract

Chitosan plays a most important role in the regenerative medication for wound healing. The adhesive nature of chitosan, with their antifungal and bactericidal character, and their permeability to oxygen, is a very important property associated with the treatment of wounds. Different derivatives and combination of chitosan have been reported for this purpose in the form of hydrogels, fibers, membranes, scaffolds and sponges. The purpose of the chapter is to have a closer look in the work done directly by different researchers on the chitosan formulation with potential medicinal applications to provide a better understanding of its usability in regenerative medicine.

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Abbreviations

PEC:

Polyelectrolyte complex

PVA:

Poly(vinyl alcohol)

AgNPs:

Silver nanoparticles

NPs:

Nanoparticles

MCNs:

Magnetic-Chitosan Nanogels

LPO:

Lactoperoxidase

E. coli:

Escherichia coli

P. aeruginosa:

Pseudomonas aeruginosa

Cu:

Copper

References

  1. Shi C, Zhu Y, Ran X, Wang M, Su Y, Cheng T (2006) Therapeutic potential of chitosan and its derivatives in regenerative medicine. J Surg Res 133:185–192

    Article  CAS  Google Scholar 

  2. Dutta PK, Dutta J, Tripathi VS (2004) Chitin chitosan: chem properties & applications. J Sci Indu Res 63:20–31

    CAS  Google Scholar 

  3. Jayakumar R, Menon D, Manzoor K, Nair SV, Tamura H (2010) Biomedical applications of chitin and chitosan based nanomaterials—A short review. Carbohydr Polym 82(2010):227–232

    Article  CAS  Google Scholar 

  4. Singh J, Dutta PK (2009) Preparation, circular dichroism induced helical conformation and optical property of chitosan acid salt complexes for biomedical applications. Int J Biol Macromol 45:384–392

    Article  CAS  Google Scholar 

  5. Mehta AS, Singh BK, Singh N, Archana D, Snigdha K, Harniman R, Rahatekar SS, Tewari RP, Dutta PK (2015) Chitosan silk-based three-dimensional scaffolds containing gentamicin encapsulated calcium alginate beads for drug administration and blood compatibility. J Biomater Appl 29:1314–1325

    Article  CAS  Google Scholar 

  6. Singh BK, Archana D, Sirohi R, Jain A, Dutta PK (2015) Porous chitosan scaffolds: A systematic study for choice of crosslinker and growth factor incorporation. Int J Polymer Mater 64:242–252

    Article  CAS  Google Scholar 

  7. Archana D, Upadhyay L, Tewari RP, Dutta J, Huang YB, Dutta PK (2013) Chitosan-pectin-alginate as a novel scaffold for tissue engineering applications. Indian J Biotechnol 12:475–4828

    CAS  Google Scholar 

  8. Muzzarelli RAA (2009) Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr Polym 76:167–182

    Article  CAS  Google Scholar 

  9. Muzzarelli RAA, Morganti P, Morganti G, Palombo P, Palombo M, Biagini G, Belmontec MM, Giantomassic F, Orlandif F, Muzzarelli C (2007) Chitin nanofibrils/chitosan glycolate composites as wound medicaments. Carbohydr Polym 70:274–284

    Article  CAS  Google Scholar 

  10. Tripathi S, Mehrotra GK, Dutta PK (2009) Preparation and physicochemical evaluation of chitosan/poly(vinyl alcohol)/pectin ternary film for food-packaging applications. Carbohyd Polym 79:711–716

    Article  Google Scholar 

  11. Singh J, Dutta PK (2010) Preparation, antibacterial & physicochemical behavior of chitosan/ofloxacin complexes. Int J Polym Mater 59:793–807

    Article  CAS  Google Scholar 

  12. Archana D, Dutta J, Dutta PK (2013) Evaluation of chitosan nano dressing for wound healing: Characterization, in vitro and in vivo studies. Int J Biol Macromol 57:193–203

    Article  CAS  Google Scholar 

  13. Archana D, Singh BK, Dutta J, Dutta PK (2013) In vivo evaluation of chitosan–PVP–titanium dioxide nanocomposite as wound dressing material. Carbohydr Polym 95:530–539

    Article  CAS  Google Scholar 

  14. Arand AG, Sawaya R (1986) Intraoperative Chem hemost Neurosurg. Neurosurg 18:223–233

    CAS  Google Scholar 

  15. Wang XH, Li DP, Wang WJ, Feng QL, Cui FZ, Xu YX, Song XH, van der Werf M (2003) Crosslinked collagen/chitosan matrices for artificial livers. Biomaterials 24:3213–3220

    Article  CAS  Google Scholar 

  16. Kojima K, Okamoto Y, Miyatake K, Kitamura Y, Minami S (1989) Collagen typing of granulation tissue induced by chitin and chitosan. Carbohydr Polym 37:109–113

    Article  Google Scholar 

  17. Harkins AL, Duri S, Kloth LC, Tran CD (2014) Chitosan-cellulose composite for wound dressing material. Part Antimicrobial activity, blood absorption ability, and biocompatibility: Chitosan-cellulose composite for wound dressing material. J Biomed Mater Res B Appl Biomater 102:1199–1206

    Article  Google Scholar 

  18. Paul W, Sharma CP (2004) Chitin and alginates wound dressings: a short review. Trends Biomater Artif Organs 18:18–23

    Google Scholar 

  19. Han F, Dong Y, Song A, Yin R, Li S (2014) Alginate/chitosan based bi-layer composite membrane as potential sustained-release wound dressing containing ciprofloxacin hydrochloride. Appl Surf Sci 311:626–634

    Article  CAS  Google Scholar 

  20. Sung JH, Hwang MR, Kim JO, Lee JH, Kim YI, Kim JH, Chang SW, Jin SG, Kim JA, Lyoo WS, Han SS, Ku SK, Yong CS, Choi HG (2010) Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan. Int J Pharm 39:2232–2240

    Google Scholar 

  21. Kim HJ, Lee HC, Oh JS, Shin BA, Oh CS, Park RD, Yang KS, Cho CS (1999) Polyelectrolyte complex composed of chitosan and sodium alginate for wound dressing application. J Biomater Sci Polym Ed 10:543–556

    Article  CAS  Google Scholar 

  22. Alemdaroğlu C, Değim Z, Celebi N, Zor F, Oztürk S, Erdogan D (2006) An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns 32:319–327

    Article  Google Scholar 

  23. Arockianathan PM, Sekar S, Kumaran B, Sastry TP (2012) Preparation, characterization and evaluation of biocomposite films containing chitosan and sago starch impregnated with silver nanoparticles. Int J Biol Macromol 50:939–946

    Article  Google Scholar 

  24. Radhakumarya C, Antontyb M et al (2011) Drug loaded thermoresponsive and cytocompatible chitosan basedhydrogel as a potential wound dressing. Carbohydr Polym 83:705–713

    Article  Google Scholar 

  25. Pawar HV, Tetteh J, Boatang JS (2013) Preparation, optimisation and characterisation of novel wound healing film dressings loaded with streptomycin and diclofenac. Colloid Surf B Biointerface 102:102–110

    Article  CAS  Google Scholar 

  26. Moura LIF, Dias AMA, Leal EC, Carvalho L, De Sousa HC, Carvalho E (2014) Chitosan-based dressings loaded with neurotensin an efficient strategy to improve early diabetic wound healing. Acta Biomater 10:843–857

    Article  CAS  Google Scholar 

  27. Kashyap M, Dutta J, Dutta PK (2011) Rheological characteristics of chitosan hydrogels in different solvent systems for wound healing applications. Asian Chitin J 7:15–20

    Google Scholar 

  28. Chien KR (2008) Regenerative medicine and human models of human disease. Nature 453:302–305

    Article  CAS  Google Scholar 

  29. Khademhosseini A, Vacanti JP, Langer R (2009) Progress in tissue engineering. Sci Am 300:64–71

    Article  CAS  Google Scholar 

  30. Place ES, Evans ND, Stevens MM (2009) Complexity in biomaterials for tissue engineering. Nat Mater 8:457–470

    Article  CAS  Google Scholar 

  31. Senni K, Pereira J, Gueniche F, Delbarre-Ladrat C, Sinquin C, Ratiskol J, Godeau G, Fischer AM, Helley D, Colliec-Jouault S (2011) Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering Mar. Drugs 9:1664–1681

    CAS  Google Scholar 

  32. Wu J, Tan H, Li L, Gao C (2009) Covalently immobilized gelatin gradients within three-dimensional porous scaffolds. Chin Sci Bull 54:3174–3180

    Article  CAS  Google Scholar 

  33. Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126

    Article  CAS  Google Scholar 

  34. Lee KY, Yuk SH (2007) Polymeric protein delivery systems. Progr Polym Sci 32:669–697

    Article  CAS  Google Scholar 

  35. Pawar SN, Edgar KJ (2012) Alginate derivatization: a review of chemistry, properties and applications. Biomaterials 33:3279–3305

    Article  CAS  Google Scholar 

  36. Tan H, Gong Y, Lao L, Mao Z, Gao C (2007) Gelatin/chitosan/hyaluronan ternary complex scaffold containing basic fibroblast growth factor for cartilage tissue engineering. J Mater Sci Mater Med 18:1961–1968

    Article  CAS  Google Scholar 

  37. Cuy JL, Beckstead BL, Brown CD, Hoffman AS, Giachelli CM (2003) Adhesive protein interactions with chitosan: Consequences for valve endothelial cell growth on tissue-engineering materials. J Biomed Mater Res A 67:538–547

    Article  Google Scholar 

  38. Dang JM, Sun DD, Shin-Ya Y, Sieber AN, Kostuik JP, Leong KW (2006) Temperature-responsive hydroxybutyl chitosan for the culture of mesenchymal stem cells and intervertebral disk cells. Biomaterials 27:406–418

    Article  CAS  Google Scholar 

  39. Cho JH, Kim SH, Park KD, Jung MC, Yang WI, Han SW, Noh JY, Lee JW (2004) Chondrogenic differentiation of human mesenchymal stem cells using a thermo sensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer. Biomaterials 25:5743–5751

    Article  CAS  Google Scholar 

  40. Agnihotri SA, Mallikarjuna NN, Aminabhavi TM (2004) Recent advances on chitosan-based micro- and nanoparticles in drug delivery. J Control Release 100:5–28

    Article  CAS  Google Scholar 

  41. Dutta P, Rinki K, Dutta J (2011) Chitosan: a promising biomaterial for tissue engineering scaffolds. Chitosan for biomaterials II. In: Jayakumar R, Prabaharan M, Muzzarelli RAA (eds) Advances in polymer science. Heidelberg: Springer Berlin, p 45–79

    Google Scholar 

  42. Jagur-Grodzinski J (2003) Biomedical applications of polymers 2001–2002. e-Polym Paper No 12

    Google Scholar 

  43. Ma Z, Lim LY (2003) Uptake of chitosan and associated insulin in Caco-2 cell monolayers: a comparison between chitosan molecules and chitosan nanoparticles. Pharm Res 20:1812–1819

    Article  CAS  Google Scholar 

  44. Tan H, Rubin JP, Marra KG (2010) Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for adipose tissue regeneration. Organogenesis 6:173–180

    Article  Google Scholar 

  45. Brannon-Peppas L (1990) Preparation and characterization of crosslinked hydrophilic networks. In: Brannon-Peppas L, Harland RS (eds) Absorbent Polymer Technology. Elsevier, Amsterdam, pp 45–66

    Chapter  Google Scholar 

  46. Peppas NA, Khare AR (1993) Preparation, structure and diffusional behavior of hydrogels in controlled release. Adv Drug Del Rev 11:1–35

    Article  CAS  Google Scholar 

  47. Jyotsna CD, Rajendra DC (2010) Formulation and evaluation of chitosan based microparticulate nasal drug delivery system of rizatriptan benzoate. Int J Pharm Tech Res 2:2391–2402

    Google Scholar 

  48. Saha P, Goyal AK, Rath G (2010) Formulation and evaluation of chitosan-based ampicillin trihydrate nanoparticles. Trop J Pharm Res 9:483–488

    Article  CAS  Google Scholar 

  49. Loke WK, Lau SK, Yong LL, Khor E, Sum CK (2000) Wound dressing with sustained anti-microbial capability. J Biomed Mater Res 53:8–17

    Article  CAS  Google Scholar 

  50. Rodrigues LB, Leite HF, Yoshida MI, Saliba JB, Cunha AS Jr, Faraco AA (2009) In vitro release and characterization of chitosan films as dexamethasone carrier. Int J Pharm 368:1–6

    Article  CAS  Google Scholar 

  51. Remuñán-Lopez C, Bodmeier RJ (1997) Mechanical water uptake and permeability properties of crosslinked chitosan glutamate and alginate films. Control J Control Release 44:215–225

    Article  Google Scholar 

  52. Yadollahia M, Farhoudiana S, Namazi H (2015) One-pot synthesis of antibacterial chitosan/silver bio-nanocomposite hydrogel beads as drug delivery systems. Int J Biol Macromol. doi:10.1016/j.ijbiomac.2015.04.032

    Google Scholar 

  53. Oyarzun-Ampuero F, Brea J, Loza M, Torres D, Alonso M (2009) Chitosan–hyaluronic acid nanoparticles loaded with heparin for the treatment of asthma. Int J Pharm 381:122–129

    Article  CAS  Google Scholar 

  54. De Campos AM, Sanchez A, Alonso MJ (2001) Chitosan nanoparticles: a new vehicle for the improvement of the delivery of drugs to the ocular surface. application to cyclosporin a. Int J Pharm 224:159–168

    Article  Google Scholar 

  55. Mo R, Jin X, Li N, Ju C, Sun M, Zhang C, Ping Q (2011) The mechanism of enhancement on oral absorption of paclitaxel by N-octyl-O-sulfate chitosan micelles. Biomaterials 32:4609–4620

    Article  CAS  Google Scholar 

  56. Jain SK, Jain NK, Gupta Y, Jain A, Jain D, Chaurasia M (2007) Mucoadhesive chitosan microspheres for non-invasive and improved nasal delivery of insulin. Indian J Pharm Sci 69:498–504

    Article  CAS  Google Scholar 

  57. Arya N, Chakraborty S, Dube N, Katti DS (2009) Electrospraying: a facile technique for synthesis of chitosan-based micro/nanospheres for drug delivery applications. J Biomed Mater Res Part B: Appl Biomater 88B:17–31

    Article  CAS  Google Scholar 

  58. Tang C, Guan YX, Yao SJ, Zhu ZQ (2014) Preparation of ibuprofen-loaded chitosan films for oral mucosal drug delivery using supercritical solution impregnation. Int J Pharm 473:434–441

    Article  CAS  Google Scholar 

  59. Changerath R, Nair PD, Mathew S, Nair CP (2009) Poly(methyl methacrylate) grafted chitosan microspheres for controlled release of ampicillin. J Biomed Mater Res B Appl Biomater 89:65–76

    Article  Google Scholar 

  60. Uskoković V, Desai TA (2014) In vitro analysis of nanoparticulate hydroxyapatite/chitosan composites as potential drug delivery platforms for the sustained release of antibiotics in the treatment of osteomyelitis. J Pharm Sci 103:567–579

    Article  Google Scholar 

  61. Cerchiara T, Abruzzo A, di Cagno M, Bigucci F, Bauer-Brandl A, Parolin C, Vitali B, Gallucci MC, Luppi B (2015) Chitosan based micro- and nanoparticles for colon-targeted delivery of vancomycin prepared by alternative processing methods. Eur J Pharm Biopharm 92:112–119

    Article  CAS  Google Scholar 

  62. Rajan M, Raj V (2013) Formation and characterization of chitosan-polylacticacid-polyethylene glycol-gelatin nanoparticles: a novel biosystem for controlled drug delivery. Carbohydr Polym 98:951–958

    Article  CAS  Google Scholar 

  63. Chen Z, Zhang L, Song Y, He J, Wu L, Zhao C, Xiao Y, Li W, Cai B, Cheng H, Li W (2015) Hierarchical targeted hepatocyte mitochondrial multifunctional chitosan nanoparticles for anticancer drug delivery. Biomaterials 52:240–250

    Article  CAS  Google Scholar 

  64. Pandey S, Mishra A, Raval P, Patel H, Gupta A, Shah D (2013) Chitosan-pectin polyelectrolyte complex as a carrier for colon targeted drug delivery. J Young Pharm. 5:160–166

    Article  CAS  Google Scholar 

  65. Unsoy G, Khodadust R, Yalcin S, Mutlu P, Gunduz U (2014) Synthesis of Doxorubicin loaded magnetic chitosan nanoparticles for pH responsive targeted drug delivery. Eur J Pharm Sci 62:243–250

    Article  CAS  Google Scholar 

  66. Jana S, Maji N, Nayak AK, Sen KK, Basu SK (2013) Development of chitosan-based nanoparticles through inter-polymeric complexation for oral drug delivery. Carbohydr Polym 98:870–876

    Article  CAS  Google Scholar 

  67. Feng C, Wang Z, Jiang C, Kong M, Zhou X, Li Y, Cheng X, Chen X (2013) Chitosan/o-carboxymethyl chitosan nanoparticles for efficient and safe oral anticancer drug delivery: in vitro and in vivo evaluation. Int J Pharm 457:158–167

    Article  CAS  Google Scholar 

  68. Zhang XZ, Tian FJ, Hou YM, Ou ZH (2015) Preparation and in vitro in vivo characterization of polyelectrolyte alginate–chitosan complex based microspheres loaded with verapamil hydrochloride for improved oral drug delivery. J Incl Phenom Macrocycl Chem 81:429–440

    Article  CAS  Google Scholar 

  69. Parveen S, Long Sahoo SK (2011) circulating chitosan/PEG blended PLGA nanoparticle for tumor drug delivery. Eur J Pharmacol 670:372–383

    Article  CAS  Google Scholar 

  70. Justin R (2014) Chen B Strong and conductive chitosan-Reduced graphene oxide nanocomposites for transdermal drug delivery. J Mater Chem B 2:3759–3770

    Article  CAS  Google Scholar 

  71. Niemirowicz K, Markiewicz KH, Wilczewska AZ, Car H (2012) Magnetic nanoparticles as new diagnostic tools in medicine. Adv Med Sci 57:196–207

    Article  CAS  Google Scholar 

  72. Lattuada M, Hatton TA (2007) Functionalization of monodisperse magnetic nanoparticles. Langmuir 23:2158–2168

    Article  CAS  Google Scholar 

  73. Shrifian-Esfahni A, Salehi MT, Nasr-esfahni M, Ekramian E (2015) Chitosan-modified superparamgnetic iron oxide nanoparticles: design, fabrication, characterization and antibacterial activity. CHEMIK 69:19–32

    Google Scholar 

  74. Zeitoun A, Robinson D, Nevo Z, Ben-Shalom N, Patchornik S (2010) Chitosan-Hybrid hydrogel technology for innovative biocompatible implants and visco-supplements in painful orthopedic pathologies, Founded 2008, at OHV Incubator, Israel

    Google Scholar 

  75. Shinde RN, Pandey AK, Acharya R, Guin R, Das SK, Rajurkarb NS, Pujari PK (2013) Chitosan-transition metal ions complexes for selective arsenic(V) preconcentration. Water Res 47:3497–3506

    Article  CAS  Google Scholar 

  76. Shanmugapriya A, Hemalatha M, Scholastica B, Prasad AAT (2013) Adsorption studies of lead (II) and nickel (II) ions on chitosan-G-polyacrylonitrile. Der Pharma Chemica 5:141–155

    CAS  Google Scholar 

  77. Manikandan A, Sathiyabama M (2015) Green synthesis of copper-chitosan nanoparticles and study of its antibacterial activity. J Nanomed Nanotechnol 6:157–7439

    Google Scholar 

  78. Tran HV, Tran LD, Ba CD, Vu HD, Nguyen TN, Phamc DG, Nguyen PX (2010) Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan-based silver nanoparticles. Colloids Surf A Physicochem Eng Asp 360:32–40

    Article  CAS  Google Scholar 

  79. Wei D, Qian W (2008) Facile synthesis of Ag and Au nanoparticles utilizing chitosan as a mediator agent. Colloids Surf B Biointerfaces 62:136–142

    Article  CAS  Google Scholar 

  80. Shahidi F, Arachchi JKV, Jeon YJ (1999) Food applications of chitin and chitosans. Trends Food Sci Tech 10:37–51

    Article  CAS  Google Scholar 

  81. Semwal A, Singh R, Dutta PK (2013) Chitosan: a promising substrate for pharmaceuticals. J Chitin Chitosan Sci 1:1–16

    Article  Google Scholar 

  82. Ko JA, Park HJ, Hwang SJ, Park JB, Lee JS (2002) Preparation and characterization of chitosan microparticles intended for controlled drug delivery. Int J Pharm 249:165–174

    Article  CAS  Google Scholar 

  83. Sahu SK, Maiti S, Pramanik A, Ghosh SK, Pramanik P (2012) Controlling the thickness of polymeric shell on magnetic nanoparticles loaded with doxorubicin for targeted delivery and MRI contrast agent. Carbohydr Polym 87:2593–2604

    Article  CAS  Google Scholar 

  84. Sadighian S, Hosseini-Monfared H, Rostamizadeh K, Hamidi M (2015) pH-triggered magnetic-chitosan nanogels (MCNs) For doxorubicin delivery: physically vs. chemically cross linking approach. Adv Pharm Bull 5:115–120

    Google Scholar 

  85. Islam MA, Firdous J, Choi YJ, Yun CH, Cho CS (2011) Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review. Int J Nanomed 7:6077–6093

    Google Scholar 

  86. Nayeri H, Fattahi A, Iranpoor-mobarakeh M, Nori P (2015) Stabilization of lactoperoxidase by tragacanth-chitosan nano biopolymer. Int J Biosci 6:418–426

    Article  Google Scholar 

  87. Singh B, Sharma V (2014) Influence of polymer network parameters of tragacanth gum-based pH responsive hydrogels on drug delivery. Carbohydr Polym 101:928–940

    Article  CAS  Google Scholar 

  88. Jayakumar R, Chennazhi KP, Muzzarelli RAA, Tamura H, Nair SV, Selvamurugan N (2010) Chitosan conjugated DNA nanoparticles in gene therapy. Carbohydr Polym 79:1–8

    Article  CAS  Google Scholar 

  89. Mohanty DP, Biswal SK, Nayak L (2015) Preparation of starch-chitosan nanocomposites for control drug release of curcumin. Inter J Curr eng Technol 5:336–31

    Google Scholar 

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Acknowledgments

One of the authors (Madhu Kashyap) gratefully acknowledged the spontaneous support and cooperation from Garm Bharti University during her research work under Ph.D.programme.

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Correspondence to Pradip Kumar Dutta .

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Kashyap, M., Archana, D., Semwal, A., Dutta, J., Dutta, P.K. (2016). Chitosan: A Promising Substrate for Regenerative Medicine in Drug Formulation. In: Dutta, P. (eds) Chitin and Chitosan for Regenerative Medicine. Springer Series on Polymer and Composite Materials. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2511-9_10

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