Structure based computational approaches are desirable to recognize and characterize protein-protein complexes and their functions. For specific proteins, the supreme fruitful technique is homology modeling whereas docking algorithms are progressively powerful and can deliver a detailed explanation of the interaction. Previous studies on modeling of hyda2 were based on different template models (Anabaena 7119 chain A, spinach PDB code 1A70, PDB code 1CZP, and Anabaena 7120 PDB code 1FRD) (Chang et al. 2007). Model produced from these templates are not considered suitable for interaction with petf as template used in these models are unmatured hydrogenase. Furthermore it was seen that, the Fe-Fe active site was not inserted due to the missing of HydE/F/G genes (Mulder et al. 2010).


Molecular Dynamic Dynamic Analysis Specific Protein Computational Approach Homology Modeling 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Chang CH, King PW, Ghirardi ML, Kim K (2007) Atomic resolution modeling of the ferredoxin:[FeFe] hydrogenase complex from Chlamydomonas reinhardtii. Biophys J 93:3034–3045CrossRefPubMedCentralPubMedGoogle Scholar
  2. Mulder DW, Boyd ES, Sarma R et al (2010) Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG). Nature 465:248–251CrossRefPubMedGoogle Scholar

Copyright information

© Springer India 2015

Authors and Affiliations

  • Pratyoosh Shukla
    • 1
  • M. V. K. Karthik
    • 2
  1. 1.Enzyme Technology and Protein Bioinformatics Laboratory Department of MicrobiologyMaharishi Dayanand UniversityRohtakIndia
  2. 2.Department of BiotechnologyBirla Institute of TechnologyRanchiIndia

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