SEM Imaging for Observation of Morphological Changes in Anaemic Human Blood Cell
Scanning Electron Microscopy (SEM) is utilized to elucidate the morphological changes in anaemic human red blood cells. Haemoglobin concentration in human blood is in the range of 11.5–13.5 g/dl in healthy adults. Haemoglobin concentration in anaemic red blood is below the lower limit of normal range. Sometimes, the nature of the abnormal shape of the blood cell determines the cause of anaemia. Normally, there occurs a variation in the diameter of the red blood cell (RBC) for different types of anaemia. Increased variation of size in blood cell is termed anisocytosis (a type of anaemia) (Mohan H, Text book of pathology, New Delhi). In case of anisocytosis, diameter of cells larger than normal cell is observed. The classification of anaemia by the size of blood cell is logical, i.e. common morphological abnormality of human blood cell (Davidson’s principle and practice of medicine, Publisher Churchill Livingstone, London). Cells are studied under ZEISS SEM with different magnification and applied potential of kV range. Thus the diameters of RBCs in SEM have been compared with RBCs photographed with light microscope. Anaemic cells are observed overlapped with each other with increasing diameter.
KeywordsScan Electron Microscopy Atomic Force Microscope Haemoglobin Concentration Central Hole Cell Morphology Change
We are indebted to Dr. Anima Sen, Ex H.O.D of USIC for her constant encouragement and endeavour to support for this entire work. We thank Prof. G. Bhattacharya, Ex H.O.D, Dept. of Biochemistry, Calcutta Medical College & Hospital, Kolkata and Prof. T.K. Das, H.O.D, Dept. of Pathology, R.G. Kar Medical College, Kolkata. We also thank Centre for Research of Nano Science & Nano Technology (CRNN), University of Calcutta for their technical support.
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