Linkage Mapping of Molecular Markers and Oligogenes

  • B. D. Singh
  • A. K. Singh


In the year 1910, the famous “fly room” of Thomas Hunt Morgan witnessed one of the major breakthroughs in the history of genetics, i.e., the discovery of the phenomenon of genetic linkage in the fruit fly Drosophila melanogaster. Linkage is a tendency of genes being inherited together as a result of their location close to each other in the same chromosome. The strength of linkage between genes is largely dependent on the distance between them in the concerned chromosome. The closer two genes are in a chromosome, the stronger is the linkage between them and the lower is the frequency of crossing over leading to the production of new allelic combinations. Based on this expectation, Sturtevant developed the first ever linkage map in the year 1913. The maps developed by Sturtevant and the subsequent researchers were based on qualitative traits often termed as phenotypic markers. The low abundance of phenotypic markers results in large gaps in their linkage maps. Advances in molecular biology and recombinant DNA technology have supported the development of molecular markers based on DNA sequence variation. These markers are abundant and can be used to reliably classify the individuals of a population into clear-cut groups and for linkage mapping. The present chapter describes different types of linkage maps, the methodologies for the construction of linkage maps using molecular markers and oligogenic traits, and the mapping functions that are used to convert the nonadditive recombination fractions into additive genetic distances. It also provides a brief discussion about the various computer software available for linkage mapping. The readers would be able to appreciate the importance of linkage maps for localizing genes and genomic regions that are associated with various traits of economic importance and their usefulness for marker-assisted selection, map-based cloning, and comparative mapping.


Genetic Distance Linkage Group Mapping Population Double Haploid Recurrent Parent 
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Copyright information

© Author(s) 2015

Authors and Affiliations

  • B. D. Singh
    • 1
  • A. K. Singh
    • 2
  1. 1.School of BiotechnologyBanaras Hindu UniversityVaranasiIndia
  2. 2.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia

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