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Development of a Multidrug Transporter Deleted Yeast-Based Highly Sensitive Fluorescent Biosensor to Determine the (Anti)Androgenic Endocrine Disruptors from Environment

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Book cover Advancements of Medical Electronics

Part of the book series: Lecture Notes in Bioengineering ((LNBE))

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Abstract

A competent and consistent androgen receptor transactivation assay has been developed using pleiotropic drug resistance transporters Pdr5, Snq2 and Yor1 deleted yeast strain, Saccharomyces cerevisiae, intended to express the human androgen receptor and androgen response element (probasin promoter) driving the expression of green fluorescent protein to determine endocrine disruptors from pulp and paper mill effluents (PPME). Stimulation of cells by known androgens, correlated with androgenic activities as measured by other reported bioassay systems. This yeast-based assay system when applied to evaluate anti-androgenic activities, the known anti-androgens effectively inhibited fluorescence reporter gene induction by dihydrotestosterone. The specificity of the assay was experienced by incubating the recombinant yeast cells with supraphysiological concentrations of non-androgenic steroidal compounds and none of them yielded considerable response. Further, the assay was used to analyze the extracted PPME from different mills confirmed strong androgenic activities. In conclusion, these results support the earlier report by us that PPME are rich in androgenic compounds and the employed detection system provides novel high throughput fluorescence based biosensor system for successful well sensitive (picogram level) screening of (anti)androgenic chemicals from various environmental sources.

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Acknowledgments

SC would like to thank CSIR and DAAD for providing research fellowship in order to carry out this work. We thank Dr. Anand Bachhawat for the vectors, Dr. M. Ghislain for the the FYAK strain and Prof. Ilpo Huhtaniemi for the kind gift of all steroidal and non-steroidal test chemicals. We thank Prof. P. Roy, Prof. C.B. Majumder and Prof. M. Höfer for their valuable technical suggestions whenever needed.

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Authors and Affiliations

  1. Department of Biotechnology, Haldia Institute of Technology, Haldia, India

    Shamba Chatterjee & Sayanta Pal Chowdhury

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  1. Shamba Chatterjee
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  2. Sayanta Pal Chowdhury
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Correspondence to Shamba Chatterjee .

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Editors and Affiliations

  1. Information Technology, JIS College of Engineering, Kalyani, West Bengal, India

    Somsubhra Gupta

  2. Biomedical Engineering, JIS College of Engineering, Kalyani, West Bengal, India

    Sandip Bag

  3. Biomedical Engineering, JIS College of Engineering, Kalyani, West Bengal, India

    Karabi Ganguly

  4. Electronics & Communication Engineering, JIS College of Engineering, Kalyani, West Bengal, India

    Indranath Sarkar

  5. Electrical Engineering, JIS College of Engineering, Kalyani, West Bengal, India

    Papun Biswas

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Chatterjee, S., Chowdhury, S.P. (2015). Development of a Multidrug Transporter Deleted Yeast-Based Highly Sensitive Fluorescent Biosensor to Determine the (Anti)Androgenic Endocrine Disruptors from Environment. In: Gupta, S., Bag, S., Ganguly, K., Sarkar, I., Biswas, P. (eds) Advancements of Medical Electronics. Lecture Notes in Bioengineering. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2256-9_15

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  • DOI: https://doi.org/10.1007/978-81-322-2256-9_15

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  • Publisher Name: Springer, New Delhi

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  • Online ISBN: 978-81-322-2256-9

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