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Recent Advances in Lichenology pp 61–94Cite as

High-Throughput Sequencing in Studies of Lichen Population Biology

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Abstract

The population genetics of lichen fungi and their photobionts have been studied for several decades. In this review, we first focus on basic questions in lichen population genetics and on recent and current experimental approaches. Then, we discuss the utility of single nucleotide polymorphism (SNP) markers and how various high-throughput sequencing (HTS) techniques that allow SNP genotyping can contribute to population studies in lichens, highlighting their respective advantages and limitations for specific types of research questions. We review the emergence of Lobaria pulmonaria as a model system in lichen population biology, enabled by the use of microsatellite markers. Finally, we discuss open questions in the field, the steps that could be taken to increase our understanding of population genetics and genomics of lichens, and the merits of HTS for determining intrathalline diversity of lichen symbionts and associated organisms. The era of HTS may turn out to be an exciting time for research in lichenology similar to the period following the invention of the microscope.

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Acknowledgments

SW was supported through a Marie Curie Fellowship (“LICHENOMICS,” #302589) within the framework of FP7 of the European Commission. SW, ÓSA, and ZOJ received funding through project grants from the Icelandic Research Fund (#120247023 and #141102-051).

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Authors and Affiliations

  1. Faculty of Life and Environmental Sciences, University of Iceland, Sturlugata 7, 101, Reykjavik, Iceland

    Silke Werth, Zophonías O. Jónsson & Ólafur S. Andrésson

  2. Swiss Federal Research Institute WSL, Zürcherstrasse 111, 8903, Birmensdorf, Switzerland

    Silke Werth

  3. Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada

    Vivian P. W. Miao

  4. Institute of Plant Sciences, University of Graz, Holteigasse 6, 8010, Graz, Austria

    Silke Werth

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  1. Silke Werth
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  2. Vivian P. W. Miao
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  3. Zophonías O. Jónsson
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  4. Ólafur S. Andrésson
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Correspondence to Silke Werth .

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Editors and Affiliations

  1. Lichenology Laboratory, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Dalip Kumar Upreti

  2. Departmento de Biologia Vegetal II Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Madrid, Spain

    Pradeep K. Divakar

  3. Lichenology Laboratory, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Vertika Shukla

  4. Lichenology Laboratory, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

    Rajesh Bajpai

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Werth, S., Miao, V.P.W., Jónsson, Z.O., Andrésson, Ó.S. (2015). High-Throughput Sequencing in Studies of Lichen Population Biology. In: Upreti, D., Divakar, P., Shukla, V., Bajpai, R. (eds) Recent Advances in Lichenology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2235-4_4

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