Plant Cytomics: Novel Methods to View Molecules on the Move

  • Eric Davies
  • Bratislav Stankovic


We provide our definition and the brief history of “cytomics” followed by an overview of general methodological approaches of optical imaging, especially fluorescence microscopy. We then go into detail on novel fluor-linking agents (nanobodies, aptamers, and aldehydes) and the array of novel fluors available. We describe many of the new techniques developed for superfast, super-resolution microscopy (photoreactivated localization microscopy, structured illumination microscopy, stimulated emission depletion microscopy, and stochastic optical reconstruction microscopy) followed by quantitative microscopy and image analysis. We then delve into unconventional methods, novel light systems, and alternatives to fluorescence (non-liner optical imaging, single-molecule light absorption, luminescent proteins). We then describe how these systems have been employed recently for proteins, nucleic acids, the cytoskeleton, and also small molecules of major interest to plants. We finish with a description of recent findings specific to plant cytomics and furnish several impressive images and other illustrations from the recent plant literature.


Green Fluorescent Protein Complementary Metal Oxide Semiconductor Yellow Fluorescent Protein Structure Illumination Microscopy Stimulate Emission Depletion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Bayesian analysis of bleaching and blinking


Charged couple devices


Cyano fluorescent protein


Complementary metal oxide semiconductor


Coherent nonlinear optical imaging


Deoxyribonucleic acid


Direct stochastic optical reconstruction microscopy


Fluorescence light sheet microscopy


Field of view


Fluorescence (or Förster) resonance energy transfer


Fluorescence photoactivation localization microscopy


Green fluorescent protein


Light-emitting diode


Nonlinear dissipation optical microscopy


Open microscopy environment remote objects


Photoactivation localization microscopy


Parametric generation spectroscopy


Plasma membrane


Pump–probe spectroscopy


Peroxy yellow 1 methyl ester


Ribonucleic acid


Reactive oxygen species


Really unconventional microscopy


Systemic evolution of ligands by exponential enrichment


Structured illumination microscopy


Soluble N-ethylmaleimide-sensitive factor-attachment proteins


Selective plane illumination microscopy




Saturated structured illumination microscopy


Stimulated emission depletion


Stochastic optical reconstruction microscopy


tandem Fluorescent protein timer


Time-reversed ultrasound encoded


Tunable light-inducible protein tag


Yellow fluorescent protein


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© Springer India 2015

Authors and Affiliations

  1. 1.Department of Plant BiologyNorth Carolina State UniversityRaleighUSA
  2. 2.University for Information Science and Technology “St. Paul the Apostle”OhridRepublic of Macedonia

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