Stem Cells and Neuronal Differentiation

  • Indrani Datta
  • Debanjana Majumdar
  • Kavina Ganapathy
  • Ramesh R. Bhonde


Stem cells are being increasingly considered alternative and viable sources of treatment for debilitating nervous system disorders and neurodegenerative diseases. Stem cells specific to nervous tissue, i.e., neural stem cells (NSCs), exist in two neurogenic regions of the adult brain – subventricular zone (SVZ) in the lateral ventricle and the subgranular zone (SGZ) in hippocampal dentate gyrus [1–4]. The inaccessibility and unavailability of NSCs deep in the brain makes it a difficult proposition to use them in clinical applications. Different stem cells are thus being tested for their neuronal differentiation capability, as a cell source for generation of functional mature neurons and glial cells. The “gold standard” of stem cells are embryonic stem cells (ESCs) as they not only retain long-term self-renewal capacity but also exhibit pluripotency to all three germ lineages. Recent advances in technology have brought the advent of another pluripotent stem cells called “inducible pluripotent stem cells” (iPSc), derived through “reprogramming” of terminally differentiated cells by the addition of a select set of genes [5–7]. However, several limitations still exist for the use of iPScs in therapeutic applications, such as the use of viral vectors for transfer of genes, inclusion of oncogenes, and teratoma formation [5, 6, 8–10]. Stem cells may also be isolated from several tissue sources and these are termed as adult stem cells (ASCs). The first ASCs to be identified were the hematopoietic stem cells (HSCs) derived from bone marrow, but the second population of stem cells from bone marrow called mesenchymal stem cells (MSCs) gained prominence due to their unique properties [11–13]. MSCs are nontumorigenic and immunomodulatory in addition to possessing multilineage differentiation potential not only towards mesodermal lineage derivatives but also to phenotypes of other germ layer cells like neuronal, hepatocytes, and islet cells [14–17]. Although fetal and adult origin MSCs possess some common characteristics with respect to expression of mesenchymal markers and absence of hematopoietic and HLA-DR markers, their neuronal differentiation efficacy is still to be evaluated for consideration as suitable candidates for nervous system disorders and neurodegenerative diseases.


Tyrosine Hydroxylase Neural Stem Cell Pluripotent Stem Cell Spinal Muscular Atrophy Neural Progenitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer India 2014

Authors and Affiliations

  • Indrani Datta
    • 1
  • Debanjana Majumdar
    • 1
  • Kavina Ganapathy
    • 1
  • Ramesh R. Bhonde
    • 1
  1. 1.School of Regenerative MedicineManipal Institute of Regenerative Medicine, Manipal UniversityBangaloreIndia

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