Considerations for Culturing and Preserving Adult Stem Cells for Therapeutics

  • Simi M. Chacko
  • Periannan Kuppusamy


The field of stem-cell therapy holds immense potential to develop as a new branch of medicine that can not only enhance endogenous stem-cell repair processes in the human body, but also be able to supplement with isolated and expanded multipotent autologous stem cells especially in the case of diseased and old patients when the body is unable to generate sufficient and healthy stem cells. In order to be able to supply clinical quality stem cells, several traditional practices that were previously used for cell culture need to be challenged and new practices established that will closely mimic physiological conditions. This review focuses on the current methods used for cryopreservation of stem cells, the source of serum and growth factors supplied during stem-cell culture, and also on the role of oxygen as an important regulatory factor that challenges to be controlled in laboratory conditions to meet physiological needs. Despite the limitations in finding safe practices for cryopreserving stem cells for the purpose of long-term use, limited availability of human supplements to grow stem cells at a clinical scale, and the extreme challenge to limit the exposure of these sensitive stem cells cultured in vitro from a naturally abundant oxygen surrounding, we discuss here the efforts of research groups that are investing in finding improved methods to optimize stem-cell protocols. It is the hope that increase in the knowledge to fine-tune these cells will open doors for healing several traditionally hard-to-treat disease conditions.


Stem Cell Adult Stem Cell Autologous Serum Hypoxic Precondition Hypoxic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Dorothy M. Davis Heart and Lung Research Institute, Department of Internal MedicineThe Ohio State UniversityColumbusUSA
  2. 2.EPR CenterGeisel School of Medicine at DartmouthLebanonUSA

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