Application of Molecular Microbiology

  • Surajit Das
  • Hirak Ranjan Dash


Biofilm is an aggregate of microorganisms with a distinct architecture where cells stick to a static surface. These cells are embedded with the self-produced matrix that consists of extracellular polymeric substances (EPS). These EPS consist of extracellular deoxyribonucleic acid (DNA), proteins and polysaccharides. Biofilm may be formed on both living and nonliving substances which is of wide concern both from the environmental as well as clinical point of view. The biofilm-forming bacterial community is distinct from planktonic cells as the planktonic cells are mostly single cells that float or swim in the liquid media. Microbial biofilm may be the result of many factors including cellular recognition for different attachment sites on a surface, nutrition deficiency or exposure of planktonic cells to subinhibitory concentration of antibiotics.


Confocal Laser Scanning Microscopy Extracellular Polymeric Substance Crystal Violet Luria Bertani Acridine Orange 
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Copyright information

© Springer India 2015

Authors and Affiliations

  1. 1.Department of Life ScienceNational Institute of TechnologyRourkelaIndia

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