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Tools and Techniques to Measure Oxidative Stress

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Free Radicals in Human Health and Disease

Abstract

Oxidative stress overwhelms the natural antioxidant defense system by creating imbalance in production and consumption of reactive oxygen species (ROS). A number of reactive molecules and free radicals exemplify the ROS. Accurate measurement of ROS may help in the diagnosis of various diseases such as diabetes, cancer, and cardiovascular diseases. The robust and sensitive assays are required for its detection and quantification. In this chapter, we describe various techniques to measure the oxidative stress by formation of oxidative by-products of lipids, proteins, and nucleic acids as well as the probing with various compounds. Methods including trapping, spectrofluorimetry, flow cytometry, ELISA, and antibody-based assays have been discussed. Understanding the tools and techniques to measure oxidative stress will help researchers to overcome various complications due to overproduction of reactive species (RS).

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Acknowledgment

We acknowledge the Department of Biotechnology, DBT (Govt. of India) for financial support. This work was supported by a research grant awarded to Dr Vibha Rani by the Department of Biotechnology (DBT), Government of India (BT/PR3978/17/766/2011).

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Authors and Affiliations

  1. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307, Uttar Pradesh, India

    Vibha Rani, Suryma Asthana, Mohit Vadhera & Neha Atale

  2. School of Life Sciences, Central University of Gujarat, Sector 30, Gandhinagar, Gujarat, 382030, India

    Umesh Chand Singh Yadav

Authors
  1. Vibha Rani
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  2. Suryma Asthana
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  3. Mohit Vadhera
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  4. Umesh Chand Singh Yadav
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  5. Neha Atale
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Correspondence to Vibha Rani .

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Editors and Affiliations

  1. Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Uttar Pradesh, India

    Vibha Rani

  2. School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India

    Umesh Chand Singh Yadav

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Rani, V., Asthana, S., Vadhera, M., Yadav, U.C.S., Atale, N. (2015). Tools and Techniques to Measure Oxidative Stress. In: Rani, V., Yadav, U. (eds) Free Radicals in Human Health and Disease. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2035-0_4

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