Connection to Anxiolytic Behavior and Reflex Movements

  • Shabnum Nabi


Some testing methods used for the assessment of the emotional state in rodents are based on the conflict between exploration and aversion, that is, on the capacity of situational aversiveness to reduce or block exploratory responses. These methods include the elevated plus maze test, the black–white transition test, and the emergence-from-cage test. The elevated plus maze apparatus consists of an elevated maze with intersecting arms, of which two are open and two are closed. This test is based on the assumption that rats and mice prefer to be in an enclosed environment, compared with an open space. This test provides information concerning anxiety-like behavior in these animals. The animal is placed in the center of the maze and has free access to all arms. Entries into open and closed arms and time spent in open and closed arms are scored by incidence. This test has been validated behaviorally and pharmacologically (Pellow et al. 1985; Pellow and File 1986). Anxiogenic compounds, such as pentylenetetrazole and FG 7142, further decrease the percentage of entries into and time spent in the open arms, whereas anxiolytic drugs, such as benzodiazepines, elicit opposite effects. The elevated plus maze test has been frequently used for the assessment of emotional changes produced in rodents by developmental exposure to psychoactive compounds. Recent results obtained in rats exposed prenatally to a benzodiazepine derivative may be cited as an example (Kellogg et al. 1991). Adult male rats exposed in utero to diazepam spent significantly more time in the open arms than did rats exposed in utero to vehicle. The total amount of time spent in either the open or the closed arms, however, was not affected by prenatal drug treatment. Such data could be interpreted as indicating a decrease in the emotional reactivity of animals exposed to diazepam during gestation.


Elevated Plus Maze Maze Test MeHg Exposure Developmental Exposure Situational Aversiveness 
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  1. Carlini VP, Varas MM, Cragnolini AB, Schioth HB, Scimonelli TN, de Barioglio SR (2004) Differential role of the hippocampus, amygdala, and dorsal raphe nucleus in regulating feeding, memory and anxiety-like behavioral responses to ghrelin. Biochem Biophys Res Commun 313:635–641PubMedCrossRefGoogle Scholar
  2. File SE, Zharkovsky A, Gulati K (1991) Effects of baclofen and nitrendipine on ethanol withdrawal responses in the rat. Neuropharmacology 30:183–190PubMedCrossRefGoogle Scholar
  3. Goswami M, Mund S, Ray A (1996) Effects of some psychotropic agents on cognitive functions in rats. Indian J Physiol Pharmacol 40:75–78PubMedGoogle Scholar
  4. Kellogg CK, Primus RJ, Bitran D (1991) Sexually dimorphic influence of prenatal exposure to diazepam on behavioral responses to environmental challenge and on Gamma-aminobutyric acid (GABA)-Stimulated chloride uptake in the brain. J Pharmacol Exp Ther 256:259–265PubMedGoogle Scholar
  5. Ken M, Takashi Y (2000) Abnormal air-righting reflex in striated rats. Jpn J Physio 50:163–166CrossRefGoogle Scholar
  6. Lister R (1987) The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology 92:180–195PubMedGoogle Scholar
  7. Magnus R (1924) Die Korperstellung. Springer, Berlin, p 740CrossRefGoogle Scholar
  8. Pellow S, File SE (1986) Anxiolytic and anxiogenic drug effects in exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacol Biochem Behav 24:525–529PubMedCrossRefGoogle Scholar
  9. Pellow S, Chopin P, File SE, Briley M (1985) Validation of open-closed arm entries in an elevated plus-maze as a measure of anxiety in rat. J Neurosci Methods 14:149–167PubMedCrossRefGoogle Scholar
  10. Rogers RJ, Cole JC (1994) The elevated plus-maze: pharmacology, methodology and ethology. In: Cooper SJ, Hendrie CA (eds) Ethology and psychopharmacology. Wiley, Chichester, pp 9–44Google Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  • Shabnum Nabi
    • 1
  1. 1.Interdisciplinary Brain Research Centre (IBRC) Jawaharlal Nehru Medical CollegeAligarh Muslim UniversityAligarhIndia

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