Toxic Responses of the Plasma Glutathione Peroxidase (GSH-Px)

  • Shabnum Nabi


Selenium (Se) acts as an antagonist to the toxic effects of many heavy metals, including mercury (Yoneda and Suzuki 1997; Falnoga and Tusek-Znidaric 2007). This essential trace element (Combs and Combo 1984) is important in many biological functions, since it is a cofactor in selenium-containing enzymes, especially in antioxidant enzymes such as glutathione peroxidase (GSH-Px) (Rotruck et al. 1973). This enzyme is effective in catalyzing the decomposition of hydrogen peroxides and lipid peroxides. When GSH-Px activity is inhibited, peroxides formed during oxidative stress can propagate cell damage. Measurement of this enzyme activity is an indirect and noninvasive method that could be used to assess oxidant stress. The activity of this enzyme has been used to assess body selenium status and nutritional requirements (Levander 1991).


Intake Level Glutathione Peroxidase Activity MeHg Exposure Plasma Glutathione Peroxidase Reference Nutrient Intake 


  1. Anderson HR, Anderson O (1993) Effects of dietary ά-tocopherol and β-carotene on lipid peroxidation induced by methylmercuric chloride in mice. Pharmacol Toxicol 73:192–201CrossRefGoogle Scholar
  2. Bulat P, Dujic I, Potkonjak B, Vidakovic A (1998) Activity of glutathione peroxidase and superoxide dismutase in workers occupationally exposed to mercury. Int Arch Occup Environ Health 71:S37–S39PubMedGoogle Scholar
  3. Burk RF, Foster KA, Greenfield PM, Kiker KW, Hannon JP (1974) Binding of simultaneously administered inorganic selenium and mercury to a rat plasma protein. Proc Soc Exp Biol Med 145(3):782–785PubMedCrossRefGoogle Scholar
  4. Chen RW, Whanger PD, Fang SC (1974) Diversion of mercury binding in rat tissues by selenium: a possible mechanism of protection. Pharmacol Res Commun 6(6):571–579PubMedCrossRefGoogle Scholar
  5. Chen RW, Lacy VL, Whanger PD (1975) Effect of selenium on methylmercury binding to subcellular and soluble proteins in rat tissues. Res Commun Pathol Pharmacol 12(2):297–308Google Scholar
  6. Chen C, Qu L, Li B, Xing L, Jia G, Wang T, Gao Y, Zhang P, Li M, Chen W, Chai Z (2005) Increased oxidative DNA damage, as assessed by urinary 8-hydroxy-2-deoxyguanosine concentrations, and serum redox status in persons exposed to mercury. Clin Chem 51:759–767PubMedCrossRefGoogle Scholar
  7. Combs GF, Combo SB (1984) The nutritional biochemistry of selenium. Annu Rev Nutr 4:257–280PubMedCrossRefGoogle Scholar
  8. Denise G, Gustavo RMB, Juliana V, Lusania MGA, Jose Pedro FA, Solange CG, Fernando B (2008) Low levels of methylmercury induce DNA damage in rats: protective effects of selenium. Arch Toxicol 83:249–254Google Scholar
  9. El-Begearmi MM, Sunde ML, Ganther HE (1977) A mutual protective effect of mercury and selenium in Japanese quail. Poult Sci 56(1):313–322PubMedCrossRefGoogle Scholar
  10. Falnoga I, Tusek-Znidaric M (2007) Selenium-mercury interactions in man and animals. Biol Trace Elem Res 119:212–220PubMedCrossRefGoogle Scholar
  11. Froseth JA, Piper RC, Carlson JR (1974) Relationship of dietary selenium and oral methylmercury to blood and tissue selenium and mercury concentrations and deficiency-toxicity signs in swine. Fed Proc 33:660Google Scholar
  12. Ganther HE (1975) Selenoproteins. Chem Scr 8A:79Google Scholar
  13. Ganther HE, Wagner PA, Sunde ML (1973) Protective effects of selenium against heavy metal toxicities. In: Hemphill D (ed) Trace substances in environmental health, vol 6. University of Missouri, Columbia, pp 247–252Google Scholar
  14. Girardi G, Elias MM (1995) Mercury chloride effects on rat renal redox enzymes activities: SOD protection. Free Radic Biol Med 18:61–66PubMedCrossRefGoogle Scholar
  15. Hoekstra WG (1975) Biochemical function of selenium and its relation to vitamin E. Fed Proc 34(11):2083–2089PubMedGoogle Scholar
  16. Levander OA (1991) Scientific rationale for the 1989 recommended dietary allowances for selenium. J Am Diet Assoc 91:1572–1576PubMedGoogle Scholar
  17. MacPherson A, Barclay MNI, Scott R, Yates RWS (1997) Loss of Canadian wheat lowers selenium intake and status of the Scottish population. In: Fischer PWF, L’Abbe MR, Cockell KA, Gibson RS (eds) Trace elements in man and animals 9: proceedings of the ninth international symposium on trace elements in man and animals. NRC Research Press, Ottawa, pp 203–205Google Scholar
  18. National Academy Press (2000) Standing Committee on the Scientific Evaluation of Dietary Reference Intakes of the Food and Nutrition Board, Institute of Medicine, the National Academics with Health Canada. Dietary reference intakes for vitamin C, vitamin E, selenium, and carotenoids. Washington DCGoogle Scholar
  19. Ohi G, Nishigaki S, Seki H, Tamura Y, Maki T (1976) Efficacy of selenium in tuna and selenite in modifying methylmercury intoxication. Environ Res 12(1):49–58PubMedCrossRefGoogle Scholar
  20. Prohaska JR, Ganther HE (1977) Interactions between selenium and methylmercury in rat brain. Chem Biol Interact 16(2):155–167PubMedCrossRefGoogle Scholar
  21. Rayman M (2000) The importance of selenium to human health. Lancet 356:233–241PubMedCrossRefGoogle Scholar
  22. Rotruck IT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hockstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179:588–590PubMedCrossRefGoogle Scholar
  23. Tran D, Moody AJ, Fisher AS, Foulkes ME, Jha AN (2007) Protective effects of selenium on mercury-induced DNA damage in mussel haemocytes. Aquat Toxicol 84:11–18PubMedCrossRefGoogle Scholar
  24. Vendemiale G, Grattagliano I, Altomare E (1999) An update on the role of free radicals and antioxidant defense in human disease. Int J Clin Lab Res 29:49–55PubMedCrossRefGoogle Scholar
  25. Wagner PA (1975) Studies on the interaction of selenium with silver and methylmercury in the rat. Ph.D. thesis, University of Wisconsin, MadisonGoogle Scholar
  26. Wagner PA, Hoekstra WG, Ganther HE (1975) Alleviation of silver toxicity by selenite in the rat in relation to tissue glutathione peroxidase. Proc Soc Exp Biol Med 148(4):1106–1110PubMedCrossRefGoogle Scholar
  27. Welsh S (1974) Physiological effects of methylmercury toxicity: interaction of methylmercury with selenium, tellurium and vitamin E. Ph.D. thesis, University of Maryland, College Park, MDGoogle Scholar
  28. WHO, Food and Agriculture Organization, International Atomic Energy Agency Expert Group (1996) Trace elements in human nutrition and health. WHO, GenevaGoogle Scholar
  29. Yoneda S, Suzuki KT (1997) Equimolar Hg-Se complex binds to selenoprotein P. Biochem Biophys Res Commun 231:7–11PubMedCrossRefGoogle Scholar

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© Springer India 2014

Authors and Affiliations

  • Shabnum Nabi
    • 1
  1. 1.Interdisciplinary Brain Research Centre (IBRC) Jawaharlal Nehru Medical CollegeAligarh Muslim UniversityAligarhIndia

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