Biochemical Estimation Techniques

  • Shabnum Nabi


Tissues were minced with sharp scissors and then thawed and homogenized with the aid of York’s homogenizer fitted with Teflon plunger in a proportion of 1:10(w/v) ice-cold phosphate buffer (50 mM; PH 7.4). Aliquots of homogenates were used for the determination of malondialdehyde (MDA), hydroperoxides, reduced glutathione (GSH), and total sulfhydryl (TSH) levels. The homogenates were centrifuged for 60 min at 10,000× g at 4 °C, and determination of superoxide dismutase (SOD) and catalase (CAT) activities were carried out with the resultant supernatant. Protein concentration was determined according to the method of Lowry et al. (1951) using purified bovine serum albumin as standard.


Xanthine Oxidase Activity Phosphomolybdic Acid Ferrous Ammonium Sulfate Potassium Thiocyanate Sodium Potassium Tartrate 
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Copyright information

© Springer India 2014

Authors and Affiliations

  • Shabnum Nabi
    • 1
  1. 1.Interdisciplinary Brain Research Centre (IBRC) Jawaharlal Nehru Medical CollegeAligarh Muslim UniversityAligarhIndia

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