Skip to main content

Fungal and Mycotoxin Contamination of Herbal Raw Materials and Prospects of Higher Plant Products as Plant-Based Preservatives During Post-Harvest Processing

  • Chapter
  • First Online:
Microbial Diversity and Biotechnology in Food Security

Abstract

Herbal drugs have been used since ancient times for prevention and treatment of diseases as well as to promote health and healing. Generally, herbal drugs are considered to be free from side effects but the poor practices of their harvesting, collection, transportation and storage often lead to extensive fungal growth and accumulation of mycotoxins. Fungal and mycotoxin contaminations are the major cause of decline of market value of herbal drug raw materials. Such contamination degrades the quality of raw materials and the medicinal value of the formulated herbal drugs. Synthetic chemical preservatives have been prescribed to control different post-harvest fungal contaminations but due to their residual and mammalian toxicities, herbal pharmaceutical industries need some safer chemicals as preservatives during post-harvest processing of herbal raw materials. Currently, several plant-derived chemicals and their formulations are practically used on a large scale as antimicrobials and are recognized as safer alternatives of synthetic chemicals. Among the higher plant products, plant essential oils, being volatile in nature, may be recommended as botanical fumigants to minimize fungal growth and mycotoxin contamination of herbal drug raw materials. The present chapter deals with an account of fungal and mycotoxin contamination of herbal raw materials and the prospective of plant-derived chemicals as preservatives during post-harvest processing of herbal raw materials.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abou-Arab AAK, Kawther MS, Tantawy MEE, Badeaa RI, Khayria N (1999) Quantity estimation of some contaminants in commonly used medicinal plants in the Egyptian market. Food Chem 67:357–363

    Article  CAS  Google Scholar 

  • Amiri A, Dugas R, Pichot AL, Bompeix G (2008) In vitro and in vitro activity of eugenol oil (Eugenia caryophylata) against four important postharvest apple pathogens. Int J Food Microbiol 126:13–19

    Article  CAS  PubMed  Google Scholar 

  • Aneesh TP, Hisham M, Sekhar MS, Madhu M, Deepa TV (2009) International market scenario of traditional Indian herbal drugs—India declining. Int J Green Pharm 3:184–190

    Article  Google Scholar 

  • Anon (2003) A dream of globalizing the TCM market. Report of the Ministry of Commerce of the People’s Republic of China

    Google Scholar 

  • Aquino S, Gonçalez E, Reis TA, Araújo1 MM, Corrêa B, Villavicencio ALCH (2007) Evaluation of fungal burden of medicinal plants submitted to gamma radiation process after 30 days. International Nuclear Atlantic Conference—INAC 2007 Santos, SP, Brazil, September 30 to October 5. AssociaçãoBrasileira de Energia Nuclear-ABEN ISBN: 978-85-99141-02-1

    Google Scholar 

  • Aziz NH, Youssef YA, El-FoulyMZ, Moussa LA (1998) Contamination of some common medicinal plant samples and spices by fungi and their mycotoxins. Bot Bull Acad Sinica 39:279–285

    Google Scholar 

  • Bang U (1995) Essential oils as fungicides and sprout inhibitors in potatoes. In: Proceedings of the EAPR Pathology Section Meeting, Phytophthorainfestancs ISO, Dublin, 10–16 September

    Google Scholar 

  • Bauer K, Garbe D, Surburg H (2001) Common fragrance and flavor materials: preparation, properties and uses. Wiley-VCH, Weinheim, pp 293

    Book  Google Scholar 

  • Benner JP (1993) Pesticidal compounds from higher plants. Pest Sci 39:95–102

    Article  Google Scholar 

  • Bhandari S, Dobhal U, Sajwan M, Bisht NS (2008) Trichosanthestricuspidata: a medicinally important plant. Trees Life 3:1–5

    Google Scholar 

  • Bhatnagar D, Garcia S (2001) Aspergillus. In: Labbe RG, Garcia S (eds) Guide to foodborne pathogens. Wiley, New York, pp 35–49

    Google Scholar 

  • Bugno A, Almodovar AAB, Pereira TC, Pinto TJA, Sabino M (2006) Occurrence of toxigenic fungi in herbal drugs. Braz J Microbiol 37:47–51

    Article  Google Scholar 

  • Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253

    Article  CAS  PubMed  Google Scholar 

  • Chang WT, Choi YH, VanderHeij- denR, Lee MS, Lin MK, Kong H, Kim K, Verpoorte R, Hanke- meierT, VanderGreef J, Wang M (2011) Traditional processing strongly affects metabolite composition by hydrolysis in Rehmanniaglutinosa roots. Chem Pharm Bull 59:546–552

    Article  CAS  PubMed  Google Scholar 

  • Chauhan YS, Ghaffar MA (2002) Solar heating of seeds a low cost method to control bruchid (Callosobruchus spp.) attack during storage of pigeon pea. J Stored Prod Res 38:87–91

    Article  Google Scholar 

  • Chourasia HK (1995) Mycobiota and mycotoxins in herbal drugs of Indian pharmaceutical industries. Mycol Res 99:697–703

    Article  CAS  Google Scholar 

  • Chu CL, Liu WT, Zhou T, Tsao R (1999) Control of post harvest gray mold rot of modified atmosphere packaged sweet cherries by fumigation with thymol and acetic acid. Can J Plant Sci 79:685–689

    Article  CAS  Google Scholar 

  • Citarasu T (2010) Herbal biomedicines: a new opportunity for aquaculture industry. Aquacult Int 18:403–414

    Article  Google Scholar 

  • Combrinck S, Bosman AA, Botha DPlooyW, McCrindle RI, Retief E (2006) Effects of post-harvest drying on the essential oil and glandular trichomes of Lippiascaberrima Sond. J Essential Oil Res 18:80–84

    Article  CAS  Google Scholar 

  • Combrinck S, Regnier T, Kamatou GPP (2011) In vitro activity of eighteen essential oils and some major components against common postharvest fungal pathogens of fruit. Ind Crops Prod 33:344–349

    Article  CAS  Google Scholar 

  • Conner DE (1993) Naturally occurring compounds. In: Davidson PM, Branen AL (eds) Antimicrobials in Food. Marcel Dekker, New York, pp 441–468

    Google Scholar 

  • Corato UD, Maccioni O, Trupo M, Sanzo GD (2010) Use of essential oil of Laurusnobilis obtained by means of a supercritical carbon dioxide technique against post harvest spoilage fungi. Crop Prot 29:142–147

    Article  Google Scholar 

  • Delgado S, Nunez F, Sanchez B, Bermudez E, Rodriguez JM (2011) Toxigenic microorganisms in medicinal plants used for ritual protection of infants. Food Res Int 44:304–309

    Article  CAS  Google Scholar 

  • Dhingra OD, Jham GN, Rodrigues FA, Silva GJ, Costa MLN (2009) Fumigation with essential oil of mustard retards fungal growth and accumulation of ergosterol and free fatty acid in stored shelled groundnuts. J Stored Prod Res 45:24–31

    Article  CAS  Google Scholar 

  • Dubey NK, Bhargava KS, Dixit SN (1983) Protection of some stored food commodities from fungi by essential oils of Ocimumcanumand Citrus medica. Int J Trop Plant Dis 1:177–179

    Google Scholar 

  • Dubey NK, Kumar R, Tripathi P (2004) Global promotion of herbal medicine: India’s opportunity. Curr Sci 86:37–41

    Google Scholar 

  • Dubey NK, Kumar A, Singh P, Shukla R (2008) Microbial contamination of raw materials: a major reason for the decline of India’s share in the global herbal market. Curr Sci 95:717–718

    Google Scholar 

  • Dubey NK, Shukla R, Kumar A, Singh P, Prakash B (2010) Prospects of botanical pesticides in sustainable agriculture. Curr Sci 98(4):479–480

    Google Scholar 

  • Efuntoye MO (1996) Fungi associated with herbal drug plants during storage. Mycopathologia 136:115–118

    Article  PubMed  Google Scholar 

  • Efuntoye MO (1999) Mycotoxins of fungal strains from stored herbal plants and mycotoxin contents of Nigerian crude herbal drugs. Mycopathologia 147:43–48

    Article  CAS  PubMed  Google Scholar 

  • Feng W, Zheng X (2007) Essential oils to control Alternaria alternata in vitro and in vivo. Food Control 18:1126–1130

    Article  CAS  Google Scholar 

  • Gautam AK, Bhadauria R (2011) Diversity of fungi and mycotoxins associated with stored Triphala churn and its ingredients. J BiolSci 11:226–235

    CAS  Google Scholar 

  • Golob P (1997) Current status and future perspectives for inert dusts for control of stored product insects. J Stored Prod Res 33:69–79

    Article  Google Scholar 

  • Halt M (1998) Moulds and mycotoxins in herb tea and medicinal plants. Eur J Epidemiol 14:269–274

    Article  CAS  PubMed  Google Scholar 

  • Hatton T, Cubbedge RH (1979) Phytotoxicity of methyl bromide as a fumigant for Florida citrus fruit. Proc Fla State Hortc Soc 92:167–169

    CAS  Google Scholar 

  • Hitokoto H, Morazumi S, Wauke T, Sakai S, Kurata H (1978) Fungal contamination and mycotoxin detection of powdered herbal drugs. Appl Environ Microbiol 36(2):252–256

    CAS  PubMed Central  PubMed  Google Scholar 

  • IARC Working Group (1993) International Agency for Research on Cancer (IARC). Some naturally occurring substances: food items and constituents. IARC monographs on the evaluation of carcinogenic risk to humans. World Health Organization, Lyon

    Google Scholar 

  • IARC Working Group (2002) International Agency for Research on Cancer. Some traditional herbal medicines, some mycotoxins, Nnaphthalene and styrene IARC monographs on the evaluation of carcinogenic risks to humans World Health Organization, vol. 82, Lyon

    Google Scholar 

  • Joseph GS, Jayaprakasha GK, Selvi AT, Jena BS, Sakariah KK (2005) Antiaflatoxigenic and antioxidant activities of Garcinia extracts. Int J Food Microbiol 101:153–160

    Article  CAS  PubMed  Google Scholar 

  • Kamboj VP (2000) Herbal medicine. Curr Sci 78(1):35–39

    Google Scholar 

  • Kumar R, Mishra AK, DubeyNK TripathiYB (2007) Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Int J Food Microbiol 115:159–164

    Article  CAS  PubMed  Google Scholar 

  • Kumar A, Shukla R, Singh P, Prasad CS, Dubey NK (2008) Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against post harvest fungal infestation of food commodities. Innov Food Sci Emerging Technol 9:575–580

    Article  CAS  Google Scholar 

  • Kumar A, Shukla R, Singh P, Dubey NK (2009) Biodeterioration of some herbal raw materials by storage fungi and aflatoxin and assessment of Cymbopogonflexuosus essential oil and its components as antifungal. Int Biodeter Biodegrad 63:712–716

    Article  CAS  Google Scholar 

  • Kumar A, Shukla R, Singh P, Dubey NK (2010) Chemical composition, antifungal and antiaflatoxigenic activities of Ocimum sanctum L. essential oil and its safety assessment as plant based antimicrobial. Food Chem Toxicol 48:539–543

    Article  CAS  PubMed  Google Scholar 

  • Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY, Kim J (2007) Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. Pt Pathol J 23:97–102

    CAS  Google Scholar 

  • Mazzanti G, Battinelli L, Daniele C, Costantini S, Ciaralli L, Evandri MG (2008) Purity control of some Chinese crude herbal drugs marketed in Italy. Food Chem Toxicol 46:3043–3047

    Article  CAS  PubMed  Google Scholar 

  • Mishra AK, Dubey NK (1994) Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl Environ Microbiol 60:1101–1105

    CAS  PubMed Central  PubMed  Google Scholar 

  • Mishra PK, Shukla R, Singh P, Prakash B, Dubey NK (2012a) Antifungal and antiaflatoxigenic efficacy of Caesuliaaxillaris Roxb. essential oil against fungi deteriorating some herbal raw materials, and its antioxidant activity. Ind Crops Prod 36:74–80

    Article  CAS  Google Scholar 

  • Mishra PK, Shukla R, Singh P, Prakash B, Kedia A, Dubey NK (2012b) Antifungal, anti-aflatoxigenic, and antioxidant efficacy of Jamrosa essential oil for preservation of herbal raw materials. Int Biodeter Biodegrad 74:11–16

    Article  CAS  Google Scholar 

  • Omidbeygi M, Barzegar M, Hamidi Z, Naghdibadi H (2007) Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control 18:1518–1523

    Article  CAS  Google Scholar 

  • Pathanadech A, Petcharat V, Chuenchit S, Lim S, Chinaputi A (2001) Aflatoxin B1-producing Aspergillus in sun-dried medicinal plant materials. Songklanakarin J Sci Technol 23:499–514

    Google Scholar 

  • Plooy W, Regnier T, Combrinck S (2009) Essential oil amended coatings as alternatives to synthetic fungicides in citrus postharvest management. Postharvest BiolTechnol 53:117–122

    Article  Google Scholar 

  • Prakash B, Shukla R, Singh P, Kumar A, Mishra PK, Dubey NK (2010) Efficacy of chemically characterized Piper betle L. essential oil against fungal and aflatoxin contamination of some edible commodities and its antioxidant activity. Int J Food Microbiol 142:114–119

    Article  CAS  PubMed  Google Scholar 

  • Prakash B, Shukla R, Singh P, Mishra PK, Dubey NK, Kharwar RN (2011) Efficacy of chemically characterized Ocimumgratissimum L. essential oil as an antioxidant and a safe plant based antimicrobial against fungal and aflatoxin B1 contamination of spices. Food Res Int 44:385–390

    Article  CAS  Google Scholar 

  • Prakash B, Singh P, Kedia A, Dubey NK (2012b) Assessment of some essential oils as food preservatives based on antifungal, antiaflatoxin, antioxidant activities and in vivo efficacy in food system. Food Res Int 49:201–208

    Article  CAS  Google Scholar 

  • Prakash B, Singh P, Mishra PK, Dubey NK (2012a) Safety assessment of Zanthoxylumalatum Roxb. essential oil, its antifungal, antiaflatoxin, antioxidant activity and efficacy as antimicrobial in preservation of Piper nigrum L. Fruits. Int J Food Microbiol 153:183–191

    Article  CAS  Google Scholar 

  • Razzaghi-Abyaneh M, Shams-Ghahfarokhi M, Yoshinari T, Rezaee MB, Jaimand K, Nagasawa H, Sakuda S (2008) Inhibitory effects of Saturejahortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus. Int J Food Microbiol 123:228–233

    Article  CAS  PubMed  Google Scholar 

  • Rizzo I, Vedoya G, Maurutto S, Haidukowski M, Varsavsky E (2004) Assessment of toxigenic fungi on Argentinean medicinal herbs. Microbiol Res 159:113–120

    Article  CAS  PubMed  Google Scholar 

  • Roy AK (2003) Mycological problems of crude herbal drugs—overview and challenges. Indian Phytopathol 56:1–13

    Google Scholar 

  • Roy AK, Chourasia HK (1990) Mycoflora, mycotoxinproducibility and mycotoxins in traditional herbal drugs from India. J General Appl Microbiol 36:295–302

    Article  CAS  Google Scholar 

  • Roy AK, Sinha KK, Chourasia HK (1988) Aflatoxin contamination of some common drug plants. Appl Environmental Microbiol 54:842–843

    CAS  Google Scholar 

  • Sa’nchez E, Heredia N, Garcı´a S (2005) Inhibition of growth and mycotoxin production of Aspergillus flavus and Aspergillus parasiticus by extracts of Agave species. Int J Food Microbiol 98:271–279

    Article  Google Scholar 

  • Sahoo N, Manchikanti P, Dey S (2010) Herbal drugs: standards and regulation. Fitoterapia 81:462–471

    Article  PubMed  Google Scholar 

  • Sharma N, Tripathi A (2006) Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World J Microbiol Biotechnol 22:587–593

    Article  CAS  Google Scholar 

  • Shukla R, Kumar A, Prasad CS, Srivastava B, Dubey NK (2008) Antimycotic and antiaflatoxigenic potency of AdenocalymmaalliaceumMiers. on fungi causing biodeterioration of food commodities and raw herbal drugs. Int Biodeter Biodegrad 62:348–351

    Article  CAS  Google Scholar 

  • Shukla R, Kumar A, Singh P, Dubey NK (2009) Efficacy ofLippia alba (Mill.) N.E. Brown essential oil and its monoterpene aldehyde constituents against fungi isolated from some edible legume seeds and aflatoxin B1 production. Int J Food Microbiol 135:165–170

    Article  CAS  PubMed  Google Scholar 

  • Shukla R, Singh P, Prakash B, Dubey NK (2012) Antifungal, aflatoxin inhibition and antioxidant activity of Callistemon lanceolatus(Sm.) Sweet essential oil and its major component 1,8-cineole against fungal isolates from chickpea seeds. Food Control 25:27–33

    Article  CAS  Google Scholar 

  • Singh P, Srivastava B, Kumar A, Dubey NK (2008) Fungal contamination of raw materials of some herbal drugs and recommendation of Cinnamomum camphora oil as herbal fungitoxicant. Microb Ecol 56:555–560

    Article  CAS  PubMed  Google Scholar 

  • Singh P, Shukla R, Kumar A, Prakash B, Singh S, Dubey NK (2010a) Effect of Citrus reticulata and Cymbopogoncitratus essential oils on Aspergillus flavus growth and aflatoxin production on Asparagus racemosus. Mycopathologia 170:195–202

    Article  Google Scholar 

  • Singh P, Shukla R, Prakash B, Kumar A, Singh S, Mishra PK, Dubey NK (2010b) Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima burm. andCitrus sinensis (L.) osbeck essential oils and their cyclic monoterpene, DL-limonene. Food Chem Toxicol 48:1734–1740

    Article  CAS  Google Scholar 

  • Tatsadjieu NL, Dongmo PMJ, Ngassoum MB, Etoa FX, Mbofung CMF (2009) Investigations on the essential oil of Lippiarugosa from Cameroon for its potential use as antifungal agent against Aspergillus flavus Link ex. Fries. Food Control 20:161–166

    Article  CAS  Google Scholar 

  • Tian J, Ban X, Zeng H, He J, Huang B, Wang Y (2011) Chemical composition and antifungal activity of essential oil from Cicutavirosa L. var. latisectaCelak. Int J Food Microbiol 145:464–470

    Article  CAS  PubMed  Google Scholar 

  • Tripathi P, Dubey NK (2004) Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruit and vegetables. Postharvest BiolTechnol 32:235–245

    Article  Google Scholar 

  • Tripathi NN, Kumar N (2007) Putranjivaroxburghii oil-A potential herbal preservative for peanuts during storage. J Stored Prod Res 43:435–442

    Article  CAS  Google Scholar 

  • Tripathi P, Dubey NK, Pandey VB (2002) Kaempferol: the antifungal principle of Acacia niloticaLinn. Del J Indian Bot Soc 81:51–54

    Google Scholar 

  • Tripathi P, Dubey NK, Banerji R, Chansouria JPN (2004) Evaluation of some essential oils as botanical fungitoxicants in management of post-harvest rotting of citrus fruits. World J Microbiol Biotechnol 20:317–321

    Article  CAS  Google Scholar 

  • Tzortzakis NG (2007) Maintaining postharvest quality of fresh produce with volatile compounds. Innov Food Sci Emerging Technol 8:111–116

    Article  CAS  Google Scholar 

  • Tzortzakis NG, Economakis CD (2007) Antifungal activity of lemongrass (Cympopogoncitratus L.) essential oil against key postharvest pathogens. Innov Food Sci Emerging Technol 8:253–258

    Article  CAS  Google Scholar 

  • Varma J, Dubey NK (1999) Prospectives of botanical and microbial products as pesticides of tomorrow. CurrSci 76:172–179

    CAS  Google Scholar 

  • Varma J, Dubey NK (2001) Efficacy of essential oils of Caesuliaaxillaris and Menthaarvensisagainst some storage pests causing biodeterioration of food commodities. Int J Food Microbiol 68:207–210

    Article  CAS  PubMed  Google Scholar 

  • Webley DJ, Harris AH (1977) A comparison of fumigants for in-bag fumigation. Trop Stored Prod Inf 33:9–18

    Google Scholar 

  • WHO (1993) Regional office for the Western Pacific, research guidelines for evaluating the safety and efficacy of herbal medicines. World Health Organisation, Manila

    Google Scholar 

  • World Health Organization (2001) General guidelines for methodologies on research and evaluation of traditional medicine. WHO, Geneva

    Google Scholar 

  • WHO (2007) WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues, WHO Library Cataloguing-in-Publication Data, Spain pp 14–15

    Google Scholar 

  • Zabka M, Pavela R, Slezakova L (2009) Antifungal effect of Pimentadioica essential oil against dangerous pathogenic and toxinogenic fungi. Ind Crops Prod 30:250–253

    Article  CAS  Google Scholar 

  • Zhan JY, Zheng KY, Zhu KY, Bi CW, Zhang WL, Du CY, Fu Q, Dong TT, Choi RC, Tsim KW, Lau DT (2011) Chemical and biological assessment of Angelica sinensis Radix after processing with wine: an orthogonal array design to reveal the optimized conditions. J Agric Food Chem 59:6091–6098

    Article  CAS  PubMed  Google Scholar 

  • Zhao Z, Liang Z, Chan K, Lu G, Lee EL, Chen H, Li L (2010) A unique issue in the standardization of Chinese materia medica. Planta Med 76:1975–1986

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nawal Kishore Dubey .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer India

About this chapter

Cite this chapter

Dubey, N., Mishra, P., Kedia, A., Prakash, B. (2014). Fungal and Mycotoxin Contamination of Herbal Raw Materials and Prospects of Higher Plant Products as Plant-Based Preservatives During Post-Harvest Processing. In: Kharwar, R., Upadhyay, R., Dubey, N., Raghuwanshi, R. (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1801-2_45

Download citation

Publish with us

Policies and ethics