Abstract
A total of 299 fungal isolates were obtained from 33 soil samples collected from different regions of Haryana. All the isolates were screened for extracellular phytase production on phytase-screening medium. Among all the isolates, 97 isolates were found positive for phytase production. After repeated screening, ten potent phytase-producing fungi were selected. Selected fungi were screened for phytase production using quantitative screening. Phytase-producing fungal isolates belonged to the genera of Aspergillus, Penicillium and Trichoderma. Aspergillus sp. 50 was found to be the best phytase producer in liquid medium.
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References
Bae HD, Yanke LJ, Cheng KJ, Selinger LB (1999) A novel staining method for detecting phytase activity. J Microbiol Methods 39(1):17–22
Boling SDD, Johnson MW, Wang ML, Parsons X, Koelkebeck CM, Zimmerman KW (2000) The effects of dietary available phosphorus levels and phytase performance of young and older laying hens. Poult Sci 79:224–230
Fiske CH, Subbarow YP (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Gargova S, Roshkova Z, Vancheva G (1997) Screening of fungi for phytase production. Biotechnol Tech 11(4):221–224
Greiner R, Konietzny U (2006) Phytase for food application. Food Technol Biotechnol 44:125–140
Howson SJ, Davis RJ (1983) Production of phytate-hydrolysing enzyme by fungi. J Enzyme Microbiol Technol 5:377–382
Kumar J, Kumar A, Ali D, Singh S, Sharma PK, Sharma DC (2011) Isolation and screening of phytase producing fungi from soil and production of phytase in submerged and solid state fermentation. Adv Plant Sci 24(1):313–316
Lee DH, Choi SU, Hwang Y (2005) Culture conditions and characterizations of a new phytase-producing fungal isolate, Aspergillus sp. L117. Mycobiology 33(4):223–229
Maga JA (1982) Phytate: its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. Crit Rev Food Sci Nutr 16:1–48
Maenz DD, Classen HL (1998) Phytase activity in small intestine brush-border membrane of the chicken. Poult Sci 77:557–564
Mitchell DB, Vogel K, Weimann BJ, Pasamontes L, van Loon APGM (1997) The phytase subfamily of histidine acid phosphatase: isolation of genes for two novel phytases from fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology 143:245–252
Mullaney EJ, Daly CB, Ullah AHJ (2000) Advances in phytase research. Adv Appl Microbiol 47:157–199
Nampoothiri KM, Tomes GJ, Roopesh K, Szakacs G, Nagy V, Soccol CR, Pandey A (2004) Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. Appl Biochem Biotechnol 118:205–214
Rao DE, Rao KV, Reddy TP, Reddy VD (2009) Molecular characterization, physicochemical properties, known and potential applications of phytases: an overview. Crit Rev Biotechnol 29(2):182–198
Shah P, Bhavsar K, Soni SK, Khire JM (2009) Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions. J Indust Microbiol Biotechnol 36:373–380
Shah KB, Trivedi R (2012) Purification and purification and characterization of an extracellular phytase from Aspergillus tamari. Int J Pharma Biosci 3(2):775–783
Shieh TR, Ware JH (1968) Survey of microorganism for the production of extracellular phytase. Appl Microbiol 16:1348–1351
Singh B, Satyanarayana T (2009) Characterization of a HAP-phytase from a thermophilic mould Sporotrichum thermophile. Bioresour Technol 100:2046–2051
Singh B, Satyanarayana T (2011) Phytases from themophilic molds: their production, characteristics and multifarious applications. Process Biochem 46(7):1391–1398
Singh B, Kaur P, Satyanarayana T (2006) Fungal phytases for improving the nutritional status of foods and combating environmental phosphorus pollution. In: Chauhan, AK, Verma A (eds) Microbes: health and environment. IK International Publishers, New Delhi pp. 289–326
Singh B, Kunze G, Satyanarayana T (2011) Developments in biochemical aspects and biotechnological applications of microbial phytases. Biotechnol Mol Biol Rev 6(3):69–87
Singh B, Sapna, Jain J, Satyanarayana T (2012) Fungal phytases for combating environmental phosphorus pollution and ameliorating the nutritional status of non-ruminants. In: Devi R, Kidwai MK, Rose, PK, Saran AK (eds) Energy-water-waste nexus for environment management. Narosa Publishing House, New Delhi, pp. 292–301
Tahir A, Mateen B, Saeed, Uslu H (2010) Studies on the production of commercially important phytase from Aspergillus niger st-6 isolated from decaying organic soil. Micol Aplic Int 22(2):51–57
Vats P, Banerjee UC (2004) Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): an overview. Enzyme Microb Technol 35:3–14
Vats P, Banerjee UC (2005) Biochemical characterization of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem. J Indust Microbiol Biotechnol 32:141–147
Vohra A, Satyanarayana T (2003) Phytases: microbial sources, production, purification, and potential biotechnological applications. Crit Rev Biotechnol 23:29–60
Wodzinski RJ, Ullah AHJ (1996) Phytase. Adv Appl Microbiol 42:263–301
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The authors wish to thank the University Grants Commission (UGC), New Delhi for providing financial assistance during the course of this investigation.
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Sapna, Jain, J., Singh, B. (2014). Production of Extracellular Phytate Hydrolyzing Enzymes by Soil Fungi. In: Kharwar, R., Upadhyay, R., Dubey, N., Raghuwanshi, R. (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1801-2_38
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DOI: https://doi.org/10.1007/978-81-322-1801-2_38
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