Abstract
The fruit-rotting fungi Penicillium expansum and Penicillium digitatum are the primary cause of postharvest losses as they cause blue mold of apples and green mold of oranges, respectively. In addition to rotting, they also contaminate food with their highly toxic chemical known as patulin, which is well known for its carcinogenic effect. In the present study, the essential oil of Ocimum gratissimum obtained by hydro-distillation was tested for its antifungal assay against P. expansum and P. digitatum in in vitro culture conditions. 500 ppm MIC of the essential oil completely inhibited the mycelial growth of both the test fungi in vitro. The inhibitory effect of the essential oil was also observed directly on the fruits, apples and oranges inoculated with P. expansum and P. digitatum spore suspensions in vivo. They were fumigated with the essential oil of O. gratissimum for 10 days and the results obtained showed potent biochemical activity up to 93 % against blue mold rot of apples and up to 75 % against green mold rot of oranges. The present study suggests use of Ocimum oil as a safe, effective and alternative means to control fruit rotting fungi.
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References
Beye F (1978) Insecticides from the vegetable kingdom. Plant Res Dev 7:13–31
Dixit SN, Tripathi NN, Tripathi SC (1978) Fungitoxicity of some seed extract. Nat Acad Sci Lett 1:287–288
Dubey NK, Tiwari TN, Mandin D, Andriamboavonjy H, Chaumout JP (2000a) Antifungal properties of O. gratissimum essential oil. Fitoterapi 71:567–569
Dubey NK, Tripathi P, Singh HB, (2000b) Prospects of some essential oils as antifungal agents. J Med Aromat Plant Sci 22:350–354
Fawcett CH, Spencer DM (1970) Plant chemotherapy with natural products. Annual Rev Phytopath 8:403–418
Garcha HC, Singh V (1980) Post-harvest diseases of fruits in Punjab. Ind Phytopath 33:42–47
Hayes AW (1980) Mycotoxin: a review of biological effects and their role in human disease. Clin Toxicol 17:45
Jacobson M, Crosby DG (1971) Naturally occurring insecticides. Marcel Dekke, New York
Lancaster MC, Jenkins FP, Philip MLJ (1961) Toxicity Associated with certain samples of groundnuts. Nature 192:1095
Mishra AK, Dubey NK (1994). Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Appl Environ Microbial 60(4):1101–1105
Natrajan KR (1989) Mycotoxin and human health. Biol Edu 6:23–27
Newberne PM, rogers AE, Wagan GN (1968) Hepatorenal lesions in rats fed low lipotrope diet and exposed to aflatoxin. J Nutri XCIV:331–342
Pandey AK, Chaudhary AR, (2001) Composition of the essential oil of Ocimum gratissimum grown in Madhya Pradesh. JMAP Sci 22 & 23:26–28
Prusky D, Keen NT (1993) Involvement of performed antifungal compound in the resistance of subtropical fruits to fungal decay. Pt Dis 77:114–119
Rashmi, Yadav BP (1999) A comparative efficacy of fungicides and plant extracts on radial growth and biomass production of Alternaria alternata. J App Biol 9:73–76
Reuveni R, Fleisher A, Putievsky E (1984) Fungistatic activity of essential oils from Ocimum basilicum chemotypes. Phytopathology 110:20–22
Richard JL, Cole RJ, Archibald SO (1989) Mycotoxin, economic and health risks. Council of Agricultural Science and Technology Report, p 116
Yang RZ, Tong CS (1988) Plants used for pest control in China. Econ Bot 42:376–406
Acknowledgments
The authors are grateful to the Central Laboratory Facility, Central Institute of Medicinal and Aromatic Plants, Lucknow, for GC and GC-MS spectra.
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Rizvi, A., Khan, M., Verma, P., Saxena, G. (2014). Biochemical Activity of Ocimum gratissimum Essential Oil Against Fruit-Rotting Fungi Penicillium expansum and Penicillium digitatum . In: Kharwar, R., Upadhyay, R., Dubey, N., Raghuwanshi, R. (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1801-2_30
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DOI: https://doi.org/10.1007/978-81-322-1801-2_30
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