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Evaluation of Antifungal Activity of Metarhizium anisopliae Against Plant Phytopathogenic Fungi

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Microbial Diversity and Biotechnology in Food Security

Abstract

An indigenous insect pathogenic fungi Metarhizium anisopliae (TK09) was isolated and evaluated for antifungal activity against phytopathogenic fungi Fusarium oxysporum, Cladosporium herbarum and Curvularia clavata. The dichloromethane (DCM) solvent was used for the extraction of fungal secondary metabolites and was assessed against plant pathogenic fungi at various concentrations ranging 500–1,200 µg/ml by agar disk diffusion method. As a result, the fungal crude metabolite showed the highest inhibitory activity against C. herbarum and lowest to F. oxysporum. Thus, the overall results suggest that isolate TK09 may be used as a control for plant pathogens and insects pest as well.

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References

  • Adrio JL, Demain AL (2003) Fungal biotechnology. Internat Microbiol 6(3):191–199

    Article  CAS  Google Scholar 

  • Ali-Shtayeh MS, Abdel-Basit M, Jamous R (2002) Distribution, occurrence and characterization of entomopathogenic fungi in agricultural soil in the Palestinian area. Mycopathologia 156:235–244

    Article  PubMed  Google Scholar 

  • Amiri B, Ibrahim L, Butt TM (1999) Antifeedant properties of destruxins and their use with the entomogenous fungus Metarhizium anisopliae for improved control of crucifer pests. Biocontrol Sci Technol 9:487–498

    Article  Google Scholar 

  • Amiri-Besheli B, Khambay B, Cameron S, Deadman M, Butt TM (2000) Inter- and intra-specific variation in destruxin production by the insect pathogenic fungus, Metarhizium, and its significance to pathogenesis. Mycoll Res 104:447–452

    Article  CAS  Google Scholar 

  • Anonymous (1999) Organic agriculture. Canadian General Standards Board, Ottawa, Ontario, Canada

    Google Scholar 

  • Ansari MA, Tirry L, Vestergaard S, Moens M (2009) Selection of a highly virulent fungal isolate Metarhizium anisopliae CLO 53 for controlling Hopliaphilanthus. J Invertebr Pathol 85(2):89–96

    Article  Google Scholar 

  • Antonio BF, Almeida JEM, Clovis L (2001) Effect of Thiamethoxam on entomopathogenic microorganisms. Neotropic Entomol 30:437–447

    Article  Google Scholar 

  • Bandani AR, Khambay BPS, Faull J, Newton R, Deadman M, Butt TM (2000) Production of efrapeptins by Tolypocladium species (Deuteromycotina: hyphomycetes) and evaluation of their insecticidal and antimicrobial properties. Mycol Res 104:537–544

    Article  CAS  Google Scholar 

  • Brimner TA, Boland GJ (2003) A review of the non-target effects of fungi used to biologically control plant diseases. Agric Ecosyst Environ 100:3–16

    Article  Google Scholar 

  • Cook RJ (1988) Biological control and holistic plant-health care in agriculture. Am J Altern Agric 3:51–62

    Article  Google Scholar 

  • Demain AL (1999) Pharmaceutically Active Secondary Metabolites of Microorganisms. Appl Microbiol Biotechnol 52(4):455–463

    Article  CAS  PubMed  Google Scholar 

  • Domsch KH, Gams W, Anderson TH (1993) Compendium of soil fungi. IHW Verlag, Eching, pp 458–459

    Google Scholar 

  • Espada A, Dreyfuss MM (1997) Effect of the cyclopeptolide 90–215 on the production of destruxins and helvolic acid by Metarhizium anisopliae. J Ind Microbiol Biotechnol 19:7–11

    Article  CAS  Google Scholar 

  • Georgianna, DR, Fedorova, ND, Burroughs JL, Dolezal AL, Bok JW (2010) Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters. Mol Plant Pathol 11:213–226

    Article  CAS  PubMed  Google Scholar 

  • Goettel MS, Inglis GD, Wraight SP (2000) Fungi. In: Lacey LA, Kaya HK (eds) Field manual of techniques in invertebrate pathology, application and evaluation of pathogens for control of insects and other invertebrate pests. Kluwer Academic Publishers, Dordrecht, pp 255–279

    Chapter  Google Scholar 

  • Gupta P, Paul MS, Sharma SN (1999) Studies on compatibility of white muscardine fungus Beauveria bassinia with neem products. Indian Phytopathol 52(3):278–280

    CAS  Google Scholar 

  • Hall RA (1981) Laboratory studies on the effects of fungicides, acaricides and insecticides on the entomopathogenic fungus, Verticillium lecanii. Entomol Exp Appl 29(1):39–48

    Article  CAS  Google Scholar 

  • Harman GE (2000) Myths and dogmas of biocontrol: changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Dis 84:377–393

    Article  Google Scholar 

  • Hassan AEM, Charnely AK (1989) Ultrastructural study of the penetration by Metarhizium anisopliae through dimilin affected cuticle of Mandusasexta. J Invertebr Pathol 54(1):117–124

    Article  Google Scholar 

  • Hatta R, Ito K, Hosaki Y, Tanaka T, Tanaka A, Yamamoto M, Akimitsu K, Tsuge T (2002) A conditionally dispensable chromosome controls host-specific pathogenicity in the fungal plant pathogen Alternaria alternate. Genetics 161:59–70

    CAS  PubMed Central  PubMed  Google Scholar 

  • Hirose E, Neves PMOJ, Zequi JAC, Martins LH, Peralta CH, Moino A Jr (2001) Effect of biofertilizers and Neem oil on the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch) Sorok. Braz Arch Biol Technol 44(4):419–423

    Article  Google Scholar 

  • Hernandez-Crespo P, Santiago-Alvarez C (1997) Entomopathogenic fungi associated with natural populations of the Moroccan locust Dociostaurus maroccanus (Thunberg) (Orthoptera: Gomphocerinae) and other acridoidea in Spain. Biocontrol Sci Technol 7:357–363

    Article  Google Scholar 

  • Johnson L (2008) Iron and siderophores in fungal–host interactions. Mycol Res 112:170–183

    Article  CAS  PubMed  Google Scholar 

  • Keller C, Maillard M, Keller J, Hostettmann K (2002) Screening of European fungi for antibacterial, antifungal, larvicidal, molluscicidal, antioxidant and free-radical scavenging activities and subsequent isolation of bioactive compounds. Pharm Boil 40:518–525

    Article  CAS  Google Scholar 

  • Kershaw MJ, Moorhouse ER, Bateman R, Reynolds SE, Charnley AK (1999) The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insects. J Invertebr Pathol 74:213–223

    Article  CAS  PubMed  Google Scholar 

  • Kodaira Y (1961) Biochemical studies on the muscardine fungi in the silkworm, Bombyxmori. J Fac Text Sci Technol Shinshu Univ Ser E 5:1–68

    Google Scholar 

  • Li DP, Holdom DG (1994) Effects of pesticides on growth and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycets). J Invertebr Pathol 63:209–211

    Article  Google Scholar 

  • Liu BL, Chen JW, Tzeng YM (2000) Production of cyclodepsipeptides destruxin A and B from Metarhizium anisopliae. Biotechnol Prog 16:993–999

    Article  CAS  PubMed  Google Scholar 

  • Miyazawa M, Honjo Y, Kameoka H (1998) Biotransformation of the sesquiterpenoid ( + )- l- gurjunene using a plant pathogenic fungus Glomerella cingulata as a biocatalyst. Phytochemistry 38(4):172–174

    Google Scholar 

  • Meitkiewski R, Gorski R (1995) Growth of selected entomopathogenic fungi species and isolates on media containing insecticides. Acta Mycol 30(1):27–33

    Google Scholar 

  • Molnar I, Gibson DM, Krasnoff SB (2010) Secondary metabolites from entomopathogenic Hypocrealean fungi. Nat Prod Rep 27(9):1241–1275

    Article  CAS  PubMed  Google Scholar 

  • Neves PMOJ, Hirose E, Techujo PT, Moino A Jr (2001) Compatibility of entomopathogenic fungi with Neonicotinoid insecticides. Neotropical Entomol 3:263–268

    Article  Google Scholar 

  • Oliveria GN, Neves PMOJ, Kawazoe LS (2003) Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations. Sci Agric 60(4):663–667

    Google Scholar 

  • Ownley BH, Windham MT (2007) Biological control of plant pathogens. In: Trigiano RN, Windham MT, Windham AS (eds) Plant pathology concepts and laboratory exercises, 2nd edn. CRC Press, New York, pp 423–436

    Google Scholar 

  • Ownley BH, Gwinn KD, Vega FE (2009) Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl 55:113–128

    Article  Google Scholar 

  • Pedras MSC, Irina ZL, Ward DE (2002) The destruxins: synthesis, biosynthesis, biotransformation, and biological activity. Phytochemistry 59:579–596

    Article  CAS  PubMed  Google Scholar 

  • Roh KB, Kim CH, Lee H, Kwon HM, Park JW (2009) Proteolyticcascade for the activation of the insect Toll pathway induced by the fungal cell wall component. J Biol Chem 284:19474–19481

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shah PA, Pell JK (2003) Entomopathogenic fungi as biological control agents. Appl Microbiol Biotechnol 61:413–423

    Article  CAS  PubMed  Google Scholar 

  • St Leger RJ, Wang C (2010) Genetic engineering of fungal biocontrol agents toachieve greater efficacy against insect pests. Appl Microbiol Biotechnol 85:901–907

    Google Scholar 

  • Sumarah MW, Adams GW, Berghout J, Slack GJ, Wilson AM, Miller JD (2008) Spread and persistence of a rugulosin-producing endophyte in Picea glaucaseedlings. Mycol Res 112:731–736

    Article  PubMed  Google Scholar 

  • Tsror L, Barak R, Sneh B (2001) Biological control of black scurf on potato under organic management. Crop Prot 20(2):145–150

    Article  Google Scholar 

  • Vanninen I, Hokkanen H (1988) Effects of pesticides on four species of entomopathogenic fungi. Ann Agri Fenn 27:345–353

    CAS  Google Scholar 

  • Vega FE, Simpkins A, Aime MC, Posada F, Peterson SW, Rehner SA, Infante F, Castillo A, Arnold AE (2010) Fungal endophyte diversity in coffee plants from Colombia, Hawai’i, Mexico, and Puerto Rico. Fungal Ecol 3:122–138

    Article  Google Scholar 

  • Vey A, Hoagland RE, Butt TM (2001) Toxic metabolites of fungal biocontrol agents. In: Butt TM, Jackson C, Magan N (eds) Fungi as biocontrol agents: Progress, problems and potential. CAB International, Bristol, pp 311–346

    Chapter  Google Scholar 

  • Wang C, Skrobek A, Butt TM (2004) Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 85:168–174

    Article  CAS  PubMed  Google Scholar 

  • Woodrin JL, Kaya HK (1988) Steinernematid and heterorhabditid nematodes: a handbook of biology and techniques. Arkansas Agricultural Experiment Station, Fayetteville

    Google Scholar 

  • Yang L, Xie JT, Jiang DH, Fu YP, Li GQ (2008) Antifungal substances produced by Penicillium oxalicum strain PY-1—potential antibiotics against plant pathogenic fungi. World J Microbiol Biotechnol 24:909–915

    Article  CAS  Google Scholar 

  • Zimmermann G (1986) The “Galleria bait method” for detection of entomopathogenic fungi in soil. Z Angew Entomol 102:213–215

    Google Scholar 

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Correspondence to K. Ravindran .

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Ravindran, K., Chitra, S., Wilson, A., Sivaramakrishnan, S. (2014). Evaluation of Antifungal Activity of Metarhizium anisopliae Against Plant Phytopathogenic Fungi. In: Kharwar, R., Upadhyay, R., Dubey, N., Raghuwanshi, R. (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1801-2_22

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