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Bacteriophages as Potential Treatment Option for Antibiotic Resistant Bacteria

  • Robert BraggEmail author
  • Wouter van der Westhuizen
  • Ji-Yun Lee
  • Elke Coetsee
  • Charlotte Boucher
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 807)

Abstract

The world is facing an ever-increasing problem with antibiotic resistant bacteria and we are rapidly heading for a post-antibiotic era. There is an urgent need to investigate alterative treatment options while there are still a few antibiotics left. Bacteriophages are viruses that specifically target bacteria. Before the development of antibiotics, some efforts were made to use bacteriophages as a treatment option, but most of this research stopped soon after the discovery of antibiotics. There are two different replication options which bacteriophages employ. These are the lytic and lysogenic life cycles. Both these life cycles have potential as treatment options. There are various advantages and disadvantages to the use of bacteriophages as treatment options. The main advantage is the specificity of bacteriophages and treatments can be designed to specifically target pathogenic bacteria while not negatively affecting the normal microbiota. There are various advantages to this. However, the high level of specificity also creates potential problems, the main being the requirement of highly specific diagnostic procedures. Another potential problem with phage therapy includes the development of immunity and limitations with the registration of phage therapy options. The latter is driving research toward the expression of phage genes which break the bacterial cell wall, which could then be used as a treatment option. Various aspects of phage therapy have been investigated in studies undertaken by our research group. We have investigated specificity of phages to various avian pathogenic E. coli isolates. Furthermore, the exciting NanoSAM technology has been employed to investigate bacteriophage replication and aspects of this will be discussed.

Keywords

Bacteriophage Therapy Antibiotic resistance Escherichia coli NanoSAM 

References

  1. 1.
    Bragg RR, Kock L (2013) Nanomedicine and infectious diseases. Ex Rev Anti-infect Ther 11:359–361CrossRefGoogle Scholar
  2. 2.
    Bragg RR, Jansen A, Coetzee M, van der Westhuizen W, Boucher CE (2013) Bacterial resistance to quaternary ammonium compounds (QAC) disinfectants. Advs. Exp Medicine, Biology: Adhikari and Thapa (Eds) Infectious Diseases and Nanomedicine I Chapter 7 Google Scholar
  3. 3.
    Alisky J, Iczkowski K, Rapoport A, Troitsky N (1998) Bacteriophages show promise as antimicrobial agents. J Infect 36:5–15CrossRefGoogle Scholar
  4. 4.
    Tenover FC, Hughes JM (1996) The challenges of emerging infectious diseases: development and spread of multiply-resistant bacterial pathogens. J Am Med Assoc 275:300–304CrossRefGoogle Scholar
  5. 5.
    Brüssow H, Hendrix RW (2002) Phage genomics: small is beautiful (Minireview). Cell 108:13–16CrossRefGoogle Scholar
  6. 6.
    Ackermann HW (2003) Bacteriophage observations and evolution. Res Microbiol 154:245–251CrossRefGoogle Scholar
  7. 7.
    Ackermann HW (2007) 5500 phages examined in the electron microscope. Arch Virol 152:227–243CrossRefGoogle Scholar
  8. 8.
    Sulakvelidze A, Alavidze Z, Morris JG Jr (2001) Bacteriophage therapy (Minireview). Antimicrob Agents Chemother 45:649–659CrossRefGoogle Scholar
  9. 9.
    Ptashne M (1992) A genetic switch. 2nd edn. Cell Press, Cambridge, pp 13–30Google Scholar
  10. 10.
    Dodd IB, Shearwin KE, Barry EJ (2005) Revisited gene regulation in bacteriophage lambda. Curr Opin Genet Dev 15:145–152CrossRefGoogle Scholar
  11. 11.
    Arkin A, Ross J, McAdams HH (1998) Stochastic kinetic analysis of developmental pathway bifurcation in phage λ-infected Escherichia coli cells. Genetics 149:1633–1648Google Scholar
  12. 12.
    Witkin E (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907Google Scholar
  13. 13.
    Schubert RA, Dodd IB, Egan JB, Shearwin KE (2007) Cro’s role in the CI-Cro bistable switch is critical for l’s transition from lysogeny to lytic development. Genes Dev 21:2461–2472CrossRefGoogle Scholar
  14. 14.
    Stern A, Sorek R (2011) The phage-host arms-race: shaping the evolution of microbes. BioEssays 33:43–51CrossRefGoogle Scholar
  15. 15.
    O’Flynn G, Ross RP, Fitzgerald GF, Coffey A (2004) Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol 70:3417–3424CrossRefGoogle Scholar
  16. 16.
    Hendrix RW, Smith MC, Burns RN, Ford ME, Hatfull GF (1999) Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc Nat Acad Sci 96:2192–2197CrossRefGoogle Scholar
  17. 17.
    Dabrowska K, Switala-Jelen K, Opolski A, Weber-Dabrowska B, Gorski A (2005) Bacteriophage penetration in vertebrates. J Appl Microbiol 98:7–13CrossRefGoogle Scholar
  18. 18.
    Carlton RM (1999) Phage therapy: past history and future prospects. Archi Immunol Ther Exp 47:267–274Google Scholar
  19. 19.
    Madigan MT, Martinko JM (2006) Essentials of virology. In: Carlson J (ed) Brock biology of microorganisms, Pearson Prentice Hall, Upper Saddle River, pp 244–245Google Scholar
  20. 20.
    Van der Westhuizen WA, Bragg RR (2012) Multiplex polymerase chain reaction for screening avian pathogenic Escherichia coli for virulence genes. Avian Pathol 41:33–40CrossRefGoogle Scholar
  21. 21.
    Swart CW, Pohl CH, Kock JLF (2013) Auger-Architectomics: introducing a new nanotechnology to infectious disease. Advs. Exp Medicine Biology: Infectious Diseases and Nanomedicine. Chapter 1 Google Scholar
  22. 22.
    van der Westhuizen WA, Kock JLF, Coetsee E, van Wyk PWJ, Swart HC, Bragg RR (2013) Investigation of the final stages of a P4-like coliphage infection in Escherichia coli through scanning, transmission and nano-scanning-auger electron microscopy (NanoSAM). Sci Res Essays 8:382–387Google Scholar
  23. 23.
    Canny GO, McCormick BA (2008) Bacteria in the intestine, helpful residents or enemies from within? Infect Immun 76:3360–3373CrossRefGoogle Scholar
  24. 24.
    Bragg RR, Coetzee L, Verschoor JA (1996) Changes in the incidence of the different serovars of Haemophilus paragallinarum in South Africa: a possible explanation for vaccination failures. Onderstepoort J Vet Res 63:217–226Google Scholar
  25. 25.
    Highlander SK, Weissenberger S, Alvarez LE, Weinstock GM, Berget PB (2006) Complete nucleotide sequence of a P2 family lysogenic bactriophage, ΦMhaA1-PHL101, from Mannheim haemolytica serotype A1. Virology 350:79–89CrossRefGoogle Scholar
  26. 26.
    Gioia J, Qin X, Jiang H, Clinkenbeard K Lo, Reggie Liu, Y Fox GE, Yerrapragada S, McLeod MP, McNeil TZ, Hemphill L, Sodergren E, Wang Q, Muzny DM, Homsi FJ, Weinstock GM, Highlander SK (2006) The genome sequence of Mannheima haemolytica A1: insights into virulence, natural competence and Pasteurellaceae phylogeny. J Bacteriol 188:7257–7266Google Scholar
  27. 27.
    Froshauer A, Silvia AM, Chidambaram M, Sharma B, Weinstock GM (1996) Sensitization of bacteria to danofloxacin by temperate prophages. Antimicrob Agents Chemother 40:1561–1563Google Scholar
  28. 28.
    Williams BJ, Golomb M, Phillips T, Brownlee J, Olson MV, Smih AL (2002) Bacteriophage HP of Haemophilus influenza. J Bacteriol 184:6893–6905CrossRefGoogle Scholar
  29. 29.
    Resch G, Kulik EM, Dietrich FS, Meyer J (2004) Complete genomic nucleotide sequence of the temperate bacteriophage AaΦ23 of Actinobacillus actinomycetemcomitans. J Bacteriol 186:5523–5528CrossRefGoogle Scholar
  30. 30.
    Morgan GJ, Hatfull GF, Casjens S, Hendrix RW (2002) Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neiserria and Deinococcus. J Mol Biol 317:337–359CrossRefGoogle Scholar
  31. 31.
    Pontarollo RA, Rioux CR, Potter AA (1997) Cloning and characterization of bacteriophage like DNA from Haemophilus sommnus homologous to phages P2 and HP1. J Bacteriol 179:1872–1879Google Scholar
  32. 32.
    Roodt Y, Bragg RR, Albertyn J (2012) Identification of prophages and prophage remnants within the genome of Avibacterium paragallinarum bacterium. Sequencing 2012:1–5. Article ID 953609, doi: 10.1155/2012/
  33. 33.
    Tinsley CR, Bille E, Nassif X (2006) Bacteriophages and pathogenicity: more than providing a toxin. Microbes Infect 8:1365–1371CrossRefGoogle Scholar
  34. 34.
    Wagner PL, Waldor MK (2002) Bacteriophage control of bacterial virulence. Infect Immun 70:3985–3993CrossRefGoogle Scholar
  35. 35.
    Prescott LM, Harley JP, Klein DA (2002) Microbiology 5th edn. McGraw Hill, Boston, pp 294–820Google Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  • Robert Bragg
    • 1
    Email author
  • Wouter van der Westhuizen
    • 1
  • Ji-Yun Lee
    • 1
  • Elke Coetsee
    • 1
  • Charlotte Boucher
    • 1
  1. 1.Department of Microbial, Biochemical and Food BiotechnologyUniversity of the Free StateBloemfonteinSouth Africa

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