Application of Plant Cell and Tissue Culture for the Production of Phytochemicals in Medicinal Plants

  • Bijaya PantEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 808)


Approximately 80 % of the world inhabitants depend on the medicinal plants in the form of traditional formulations for their primary health care system well as in the treatment of a number of diseases since the ancient time. Many commercially used drugs have come from the information of indigenous knowledge of plants and their folk uses. Linking of the indigenous knowledge of medicinal plants to modern research activities provides a new reliable approach, for the discovery of novel drugs much more effectively than with random collection. Increase in population and increasing demand of plant products along with illegal trade are causing depletion of medicinal plants and many are threatened in natural habitat. Plant tissue culture technique has proved potential alternative for the production of desirable bioactive components from plants, to produce the enough amounts of plant material that is needed and for the conservation of threatened species. Different plant tissue culture systems have been extensively studied to improve and enhance the production of plant chemicals in various medicinal plants.


Medicinal plants Propagation Conservation Tissue culture 



All the tissue culture photographs are used from our research work. The author gratefully acknowledges all the team members involved in tissue culture research project.


  1. 1.
    Schippmann U, Danna L, Cunningham AB (2006) A comparison of cultivation and wild collection of medicinal and aromatic plants under sustainability aspects. In: Bogers RJ, Craker LE, Lange D (eds) Medicinal and aromatic plants. Springer, New YorkGoogle Scholar
  2. 2.
    Bannerman RH (1982) Traditional medicine in modern health care. World Health Forum 3(1):8–13Google Scholar
  3. 3.
    Sarker KP, Islam A, Islam R, Hoque A, Joarder I (1996) In-vitro propagation of Rauvolfia serpentina L. Benth. ex Kurz. through tissue culture. Planta Med 62:358–359CrossRefGoogle Scholar
  4. 4.
    Vieira RF, Skorupa LA (1993) Brazilian medicinal plants gene bank. Acta Hortic 30:51–58Google Scholar
  5. 5.
    Farnsworth NR (1966) Biological and phytochemical screening of plants. J Pharm Sci 55(3):225–276CrossRefGoogle Scholar
  6. 6.
    Balandrin MJ, Klocke JA (1988) Medicinal, aromatic and industrial materials from plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Springer, Heidelberg, pp 1–36Google Scholar
  7. 7.
    Kumar S, Hassan SA, Dwived S, Kukreja AK, Sharma A, Singh AK, Sharma S, Tewari R (2000) Proceedings of the national seminar on the frontiers of research and development in medicinal plants. J Med Aromatic Plants Sci 22(4A):461–463Google Scholar
  8. 8.
    Senghi P (2001) Ethnobotanical approaches of traditional medicine studies: some experiences from Asia. Pharm Biol 39:74–79Google Scholar
  9. 9.
    Pant B, Swar S, Gurung R (2007) Current Status and ex situ conservation of threatened orchids of Nepal. In: Proceedings of 9th Asia Pacific Orchid Conference (APOC 9), Seol, KoreaGoogle Scholar
  10. 10.
    Erdei I, Kiss Z, Maliga P (1981) Rapid clonal multiplication of Digitalis lanata in tissue culture. Plant Cell Rep 1:34–35CrossRefGoogle Scholar
  11. 11.
    Nishioka I (1988) Clonal multiplication of medicinal plants by tissue culture. Shoyakugaku Zasshi 42:1–11Google Scholar
  12. 12.
    Tsay HS, Huang HL (1998) Somatic embryo formation and germination from immature embryo—derived suspension—cultured cells of Angelica sinensis (Oliv.) Diels. Plant Cell Rep 17:670–674CrossRefGoogle Scholar
  13. 13.
    Pant B, Kohda H, Namera A (1996) Clonal propagation of Cnidium officinale by shoot tip culture. Planta Med 62:281–283CrossRefGoogle Scholar
  14. 14.
    Nongdam P, Chongtham N (2011) In vitro rapid propagation of Cymbidium aloifolium (L.) Sw.: a medicinally important orchid via seed culture. J Biol Sci 11(3):254–260CrossRefGoogle Scholar
  15. 15.
    Pant B, Thapa D (2012) In vitro mass propagation of an epiphytic orchid Dendrobium primulinum Lindl. through shoot tip culture. Afr J Biotechnol 11(42):9970–9974Google Scholar
  16. 16.
    Pradhan S, Paudel YP, Pant B (2013) Efficient regeneration of plants from shoot tip explants of Dendrobium densiflorum Lindl., a medicinal orchid. Afr J Biotechnol 12(12):1378–1383Google Scholar
  17. 17.
    Hiraoka N, Kodama T, Oyanagi M, Nakano S, Tomita Y, Yamada N, Iida O, Satake M (1986) Characteristics of Bupleurum falcatum plants propagated through somatic embryogenesis of callus cultures. Plant Cell Rep 5:319–321CrossRefGoogle Scholar
  18. 18.
    Kitamura Y, Miura H, Sugii M (1989) Plant regeneration from callus cultures of Swertia pseudochinensis. Shoyakugaku Zasshi 43:256–258Google Scholar
  19. 19.
    Sagare AP, Lee YL, Lin TC, Chen CC, Tsay HS (2000) Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae) a medicinal plant. Plant Sci 160:139–147CrossRefGoogle Scholar
  20. 20.
    Yamada Y, Shoyama Y, Nishioka I, Kohda H, Namera A, Okamoto T (1991) Clonal micropropagation of Gentiana scabra Bunge var. buergeri Maxim and examination of the homogeneity concerning the gentiopicroside content. Chem Pharm Bull 39:204–220CrossRefGoogle Scholar
  21. 21.
    Ramachandra RS, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153CrossRefGoogle Scholar
  22. 22.
    Dörnenburg H (2010) Cyclotide synthesis and supply: From plant to bioprocess. Biopolymers Pept Sci 94(5):602–610CrossRefGoogle Scholar
  23. 23.
    Dörnenburg H, Knorr D (1996) Generation of colors and flavors in plant cell and tissue cultures. Crit Rev Plant Sci 15:141–168CrossRefGoogle Scholar
  24. 24.
    Ushiyama K (1996) Commercial production of ginseng from plant cell cultures. Institute of Food Technologists Annual Meeting, New OrleansGoogle Scholar
  25. 25.
    Abdin MZ, Kamaluddin A (2006) Traditional systems of medicine: improving quality of medicinal herbs through physico-chemical and molecular approaches. India Publishing House Pvt. Ltd, New DelhiGoogle Scholar
  26. 26.
    Dörnenburg H, Knorr D (1997) Challenges and opportunities for metabolite production from plant cell and tissue cultures. Food Technol 51:47–54Google Scholar
  27. 27.
    Endo T, Yamada Y (1985) Alkaloid production in cultured roots of three species of Duboisia. Phytochemistry 24:1233–1236CrossRefGoogle Scholar
  28. 28.
    Miura Y, Hirata K, Kurano N, Miyamoto K, Uchida K (1988) Formation of vinblastine in multiple shoot culture of Cathranthus rosus. Planta Med 54:18–20CrossRefGoogle Scholar
  29. 29.
    Davioud E, Kan C, Hamon J, Tempe J, Husson HP (1989) Production of indole alkaloids by in vitro root cultures from Catharanthus trichophyllus. Phytochemistry 28:2675–2680CrossRefGoogle Scholar
  30. 30.
    Li W, Li M, Yang DL, Xu R, Zhang Y (2009) Production of podophyllotaxin by root culture of Podophyllum hexandrum Royle. Electron J Biol 5:34–39Google Scholar
  31. 31.
    Sugimoto Y, Sugimura Y, Yamada Y (1988) Effects of culture conditions on roots of Stephania cepharantha. Agric Biol Chem 52:1495–1498CrossRefGoogle Scholar
  32. 32.
    Shimomura K, Kitazawa T, Okamura N, Yagi A (1991) Tanshinone production in adventitious roots and regenerates of Salvia miltiorrhiza. J Nat Prod 54:1583–1586CrossRefGoogle Scholar
  33. 33.
    Lee YL, Sagare AP, Lee CY, Feng HT, Ko YC, Shaw JF, Tsay HS (2001) Formation of protoberberine-type alkaloids by the tubers of somatic embryo-derived plants of Corydalis yanhusuo. Planta Med 67:839–842CrossRefGoogle Scholar
  34. 34.
    Chueh FS, Chen CC, Sagare AP, Tsay HS (2000) Quantitative determination of secoiridoid glucoside in in vitro propagated plants of Gentiana davidii var. formosana by high performance liquid chromatography. Planta Med 67:70–73CrossRefGoogle Scholar
  35. 35.
    Gao SL, Zhu DN, Cai ZH, Jiang Y, Xu DR (2004) Organ culture of a precious Chinese medicinal plant—Fritillaria unibracteata. Plant Cell Tiss Org Cult 59:197–201CrossRefGoogle Scholar
  36. 36.
    King PJ (1984) Induction and maintenance of cell suspension cultures. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants. Academic Press, New YorkGoogle Scholar
  37. 37.
    Wickremesinhe ERM, Arteca RN (1993) Taxus callus cultures: initiation, growth optimization, characterization and taxol production. Plant Cell Tiss Org Cult 35:181–193CrossRefGoogle Scholar
  38. 38.
    Baumert A, Groger D, Kuzovkina IN, Reisch J (1992) Secondary metabolites produced by callus cultures of various Ruta species. Plant Cell Tiss Org Cult 28:159–162CrossRefGoogle Scholar
  39. 39.
    Thengane SR, Kulkarni DK, Shrikhande VA, Joshi SP, Sonawane KB, Krishnamurthy KV (2003) Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida. Plant Cell Tiss Org Cult 72:247–251CrossRefGoogle Scholar
  40. 40.
    Shrestha SK, Pant B (2012) Production of Bergenin, an active chemical constituent in the callus of Bergenia ciliata (Haw.) Sternb. Bot orientalis 8:40–44Google Scholar
  41. 41.
    Paudel S, Adhikari SR, Pant B (2013) Effect of colchicine on production of secondary metabolites from callus of Withania somnifera (L.) Dunal. J Nepal Biotechnol Assoc 3(1):15–18Google Scholar
  42. 42.
    Yoshikawa T, Furuya T (1985) Morphinan alkaloid production by tissues differentiated from cultured cells of Papaver somniferum. Planta Med 2:110–113CrossRefGoogle Scholar
  43. 43.
    Furuya T, Ikuta A, Syono K (1972) Alkaloids from callus cultures of Papaver somniferum. Phytochemistry 11:3041–3044CrossRefGoogle Scholar
  44. 44.
    Wickremesinhe ERM, Arteca RN (1994) Taxus cell suspension cultures: optimizing growth and production of taxol. J Plant Physiol 144:183–188CrossRefGoogle Scholar
  45. 45.
    Cragg GM, Schepartz SA, Suffuess M, Grever MR (1993) The taxol supply crisis: new NCI policies for handling the large-scale production of novel natural product anticancer and anti-HIV agents. J Nat Prod 56:1657–1668CrossRefGoogle Scholar
  46. 46.
    Srinivasan V, Pestchanker L, Moser S, Hirasuma T, Taticek RA, Shuler ML (1995) Taxol production in bioreactors; kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspensions of Taxus baccata. Biotechnol Bioeng 47:666–676CrossRefGoogle Scholar
  47. 47.
    Kim JH, Yun JH, Hwang YS, Byun SY, Kim DI (1995) Production of taxol and related taxanes in Taxus brevifolia cell cultures. Biotechnol Lett 17(1):101–106CrossRefGoogle Scholar
  48. 48.
    Lee CY, Lin FL, Yang CT, Wang LH, Wei HL, Tsay HS (1995) Taxol production by cell cultures of Taxus mairei. In: Proceedings of symposium on development and utilization of resources of medicinal plants in Taiwan, vol 48. Taiwan Agricultural Research Institute, Taiwan, 21 April, pp 137–148 (TARI Special Publication)Google Scholar
  49. 49.
    Tal B, Rokem JS, Goldberg I (1983) Factors affecting growth and product formation in plant cells grown in continuous culture. Plant Cell Rep 2:219–222CrossRefGoogle Scholar
  50. 50.
    Van Hengal AJ, Harkes MP, Witchers HJ, Hesselinic PGM, Buitglaar RM (1992) Characterization of callus formation and camptothecin production by cell lines of Camptotheca acuminata. Plant Cell Tiss Org Cult 28:11–18CrossRefGoogle Scholar
  51. 51.
    Furuya T, Yoshikawa T, Orihara Y, Oda H (1984) Studies of the culture conditions for Panax ginseng cells in jar fermentors. J Nat Prod 47:70–75CrossRefGoogle Scholar
  52. 52.
    Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226–231CrossRefGoogle Scholar
  53. 53.
    Valluri JV (2009) Bioreactor production of secondary metabolites from cell cultures of periwinkle and sandalwood. Methods Mol Biol 547:325–335CrossRefGoogle Scholar
  54. 54.
    Cheetham PSJ (1995) Biotransformations: new routes to food ingredients. Chem Ind 7:265–268Google Scholar
  55. 55.
    Scragg AH (1997) The production of aromas by plant cell cultures. In: Schepier T (ed) Advance biochemical and engineering biotechnology. Springer, BerlinGoogle Scholar
  56. 56.
    Krings U, Berger RG (1998) Biotechnological production of flavours and fragrances. Appl Microb Biotechnol 49:1–8CrossRefGoogle Scholar
  57. 57.
    Verma P, Mathur AK, Srivastava A, Mathur A (2012) Emerging trends in research on spatial and temporal organization of terpenoid indole alkaloid pathway in Catharanthus roseus: literature update. Protoplasma 249(2):255–268CrossRefGoogle Scholar
  58. 58.
    Lindsey K (1995) Manipulation by nutrient limitation of the biosynthetic activity of immobilized cells of Capsicum frutescens Mill. ev. annum. Planta 165:126–133CrossRefGoogle Scholar
  59. 59.
    Vanisree M, Chen YL, Shu-Fung L, Satish MN, Chien YL, Hsin-Sheng T (2004) Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bott Bull Acad Sin 45:1–22Google Scholar
  60. 60.
    Wink M, Alfermann AW, Franke R, Wetterauer B, Distl M, Windhovel J (2008) Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents. Plant Genetic Resour 12:113–123Google Scholar
  61. 61.
    Jin JH, Shin JH, Kim JH, Chung IS, Lee HJ (1999) Effect of chitosan elicitation and media components on the production of anthraquinone colorants in madder (Rubia akane Nakai) cell culture. Biotechnol Bioprocess Eng 4:300–304CrossRefGoogle Scholar
  62. 62.
    Zhao J, Hu Q, Guo Q, Zhu WH (2001) Effects of stress factors, bioregulators, and synthetic precursor on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus. Appl Microbial Biotechnol 55:693–698CrossRefGoogle Scholar
  63. 63.
    Staniszewska I, Krolicka A, Mali E, Ojkowska E, Szafranek J (2003) Elicitation of secondary metabolites in in vitro cultures of Ammi majus L. Enzyme Microbiol Technol 33:565–568CrossRefGoogle Scholar
  64. 64.
    Jeong GA, Park DH (2006) Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors. Appl Biochem Biotechnol 129:436–446CrossRefGoogle Scholar
  65. 65.
    Minami E, Kuchitsu K, He DY, Kouchi H, Midoh N, Ohtsuki Y, Shibuya N (1996) Two novel genes rapidly and transiently activated in suspension-cultured rice cells by treatment with N-acetylchitoheptaose, a biotic elicitor for phytoalexin production. Plant Cell Physiol 37(4):563–567CrossRefGoogle Scholar
  66. 66.
    Jeandet P, Clément C, Courot E, Cordelier S (2013) Modulation of phytoalexin biosynthesis in engineered plants for disease resistance. Int J Mol Sci 14(7):14136–14170CrossRefGoogle Scholar
  67. 67.
    Karuppusamy S (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plants Res 3:1222–1239Google Scholar
  68. 68.
    Li M, Peebles CA, Shanks JV, San KY (2011) Effect of sodium nitroprusside on growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root cultures. Biotechnol Prog 27(3):625–630CrossRefGoogle Scholar
  69. 69.
    Shanks JV, Morgan J (1999) Plant hairy root culture. Curr Opin Biotechnol 10:151–155CrossRefGoogle Scholar
  70. 70.
    Xu H, Kim YK, Suh SY, Udin MR, Lee SY, Park SU (2008) Deoursin production from hairy root culture of Angelica gigas. J Korea Soc Appl Biol Chem 51:349–351CrossRefGoogle Scholar
  71. 71.
    Asano T, Kobayashi K, Kashihara E, Sudo H, Sasaki R, Iijima Y, Aoki K, Shibata D, Saito K, Yamazaki M (2013) Suppression of camptothecin biosynthetic genes results in metabolic modification of secondary products in hairy roots of Ophiorrhiza pumila. Phytochemistry 91:128–139CrossRefGoogle Scholar
  72. 72.
    Flores HE, Hoy MW, Pickard JJ (1987) Secondary metabolites from root culture. Trends Biotechnol 5:64–69CrossRefGoogle Scholar
  73. 73.
    Spencer A, Hamill JD, Rhodes MJC (1990) Production of terpenes by differentiated shoot of Mentha citrate transformed with Agrobacterium tummifacience T-37. Plant Cell Rep 8:601–604CrossRefGoogle Scholar
  74. 74.
    Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1–22CrossRefGoogle Scholar
  75. 75.
    Van Pee KH (2011) Transformation with tryptophan halogenase genes leads to the productionof new chlorinated alkaloid metabolites by a medicinal plant. Chem Biochem 12(5):681–683Google Scholar
  76. 76.
    Kinney AJ (1998) Manipulating flux through plant metabolic pathways. Curr Opin Plant Biol 1:173–178CrossRefGoogle Scholar
  77. 77.
    Sato F, Hashimoto T, Hachiya A, Tamura K, Choi KB, Morishige T (2001) Metabolic engineering of plant alkaloid biosynthesis. Proc Natl Acad Sci 2:367–372CrossRefGoogle Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Central Department of BotanyTribhuvan UniversityKirtipurNepal

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