Functional Genomics



Functional genomics play important role in plant improvement In tea, functional genomics work was initiated with the isolation of chalcone synthase gene from a Japanese green tea cultivar ‘Yabukita’ during 1994. Since then a significant amount of genomic work has been done in tea and its wild relatives targeting isolation and characterization of trait-specific genes and their expression under varying conditions. It has been evident that two types of efforts have been made: (i) cloning of individual gene associated with particular trait and (ii) differential gene expression which leads to identification of genes that are associated to a trait. Details accounts of functional genomics work along with application of modern tools such as metabolomics, proteomics, system biology as well as bioinformatics are described in this chapter.


Suppression Subtractive Hybridization Floral Aroma Suppression Subtractive Hybridization Library Catechin Content Purine Alkaloid 


  1. Ashihara H, Kubota H (1987) Biosynthesis of purine alkaloids in Camellia plants. Plant Cell Physiol 28:535–539Google Scholar
  2. Ashihara H, Gillies FM, Crozier A (1997) Metabolism of caffeine and related purine alkaloids in leaves of tea (Camellia sinensis L.). Plant Cell Physiol 38:413–419CrossRefGoogle Scholar
  3. Ashihara H, Monterio AM, Gillies FM, Crozier A (1996) Biosynthesis of caffeine in leaves of coffee. Plant Physiol 111:747–753PubMedCentralPubMedGoogle Scholar
  4. Ashihara H, Deng WW, Mullen W, Crozier A (2010) Distribution and biosynthesis of flavan-3-ols in Camellia sinensis seedlings and expression of genes encoding biosynthetic enzymes. Phytochem 71:559–566.CrossRefGoogle Scholar
  5. Anon (2005) L-Theanine. Alternat Med Rev 10:136–138.Google Scholar
  6. Borchetia S, Bora C, Gohain B, Bhagawati P, Agarwala N, Bhattacharya N, Bharalee R, Bhorali P, Bandyopadhyay T, Gupta S, Das SK, Singh HR, Ahmed P, Gogoi M, Das S (2011) Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica). Afr J Biotech 10:5934–5939Google Scholar
  7. Borthakur D, Du YY, Chen H, Lu JL, Lin C, Dong JJ, Ye JH, Zheng XQ, Liang YR (2008) Cloning and characterization of a cDNA encoding phytoene synthase (PSY) in tea. Afr J Biotech 7:3577–3581Google Scholar
  8. Chen L, Zhao LP, Gao QK (2005) Generation and analysis of expressed sequence tags from the tender shoots cDNA library of tea plant (Camellia sinensis). Plant Sci 168:359–363CrossRefGoogle Scholar
  9. Chen Q, Yang L, Ahmad P, Wan X, Hu X (2011) Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation. Planta 233:583–592PubMedCrossRefGoogle Scholar
  10. Chen X, Fang WP, Zou Z, Wang YH, Cheng H, Li XH (2009) Cloning and expression analysis of CBF gene in cold induced tea plant [Camellia sinensis (L.) O. Kuntze]. J Tea Sci 29:53–59Google Scholar
  11. Choi JY, Mizutani M, Shimizu BI, Kinoshita T, Ogura M, Tokoro K, Lin ML, Sakata K (2007) Chemical profiling and gene expression profiling during manufacturing process of Taiwan oolong tea, “Oriental Beauty”. Biosci Biotech Biochem 71:1476–1486CrossRefGoogle Scholar
  12. Das A, Mondal TK (2010) In silico analysis of miRNA and their targets in tea. Amer J of. Plant Sci 1:77–86Google Scholar
  13. Das A, Das S, Mondal TK (2012) Identification of differentially expressed gene profiles in young roots of tea [Camellia sinensis (L.) O. Kuntze] subjected to drought stress using suppression subtractive hybridization. Plant Mol Biol Repo 30:1088–1101CrossRefGoogle Scholar
  14. Das A, Saha D, Mondal TK (2013) An optimized method for extraction of RNA from tea roots for functional genomics analysis. Indian J Biotech 12:129–132Google Scholar
  15. Deng WW, Ogita S, Ashihara H (2008) Biosynthesis of theanine (γ-ethylamine –L-Glutamine acid) in seedling of Camellia sinensis. Phytochem Lett 1:115–119CrossRefGoogle Scholar
  16. Deng W, Wang S, Chen Q, Zhang Z, Hu X (2012) Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings. Plant Physiol Biotech 56:35–40CrossRefGoogle Scholar
  17. Deng W-W, Fei Y, Wang S, Wan X-C, Zhang Z-Z, Hu X-Y (2013a) Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings. Plant Growth Regul. doi:10.1007/s10725–013-9828–1Google Scholar
  18. Deng WW, Zhang M, Wu JQ, Jiang ZZ, Tang L, Li YY, Wei CL, Jiang CJ, Wan XC (2013b) Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis. J Plant Physiol 170:272–282CrossRefGoogle Scholar
  19. Edwards JW, Walker EL, Coruzzi GM (1990) Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthase. Proc Nat Acad Sci 87:3459–3463PubMedCrossRefGoogle Scholar
  20. Eungwanichayapant PD, Popluechai S (2009) Accumulation of catechins in tea in relation to accumulation of mRNA from genes involved in catechin biosynthesis. Plant Physiol Biotech 47:94–97CrossRefGoogle Scholar
  21. Fang WP, Jiang CJ, Yu M, Ye AH, Wan ZX (2006) Differentially expression of Tua 1, a tubulin encoding gene, during flowering of tea plant Camellia sinensis (L.) O. Kuntze using cDNA amplified fragment length polymorphism technique. Acta Biochim Biophys Sin 38:653–662PubMedCrossRefGoogle Scholar
  22. Fang W, Zhang Y, Zhou L, Wang W, Li X (2013) Isolation and characterization of Histone1 gene and its promoter from tea plant (Camellia sinensis). Mole Biol Repo 40:3641–3648CrossRefGoogle Scholar
  23. Fang W, Zou Z, Hou X, Zhang D, Duan Y, Yang Y, Li X (2009) Cloning and sequence analysis of cold-induced H1-histone gene from Camellia sinensis. Acta Bot Boreali-Occidentalia Sin 8:1514–1519Google Scholar
  24. Feng YF, Liang YR (2001) Cloning and sequencing of S-adenosylmethionine synthetase gene in tea plant. J Tea Sci 21:21–25Google Scholar
  25. Fu J (2012) Molecular cloning and expression analysis of a putative sesquiterpene synthase gene from tea plant (Camellia sinensis). Acta Physiol Plant. doi:10.1007/s11738Google Scholar
  26. Gohain B, Borchetia S, Bhorali P, Agarwal N, Bhuyan LP, Rahman A, Sakata K, Mizutani M, Shimizu B, Gurusubramaniam G, Ravindranath R, Kalita MC, Hazarika M, Das S (2012) Understanding Darjeeling tea flavour on a molecular basis. Plant Mol Biol 78:577–597PubMedCrossRefGoogle Scholar
  27. Gupta S, Bharalee R, Bhorali P, Bandyopadhyay T, Gohain B, Agarwal N, Ahmed P, Saikia H, Borchetia S, Kalita MC, Handique K, Das S (2012) Identification of drought tolerant progenies in tea by gene expression analysis. Funct Integ Genom 12:543–563CrossRefGoogle Scholar
  28. Gupta S, Bharalee R, Bhorali P, Das SK, Bhagawati P, Bandyopadhyay T, Gohain B, Agarwal N, Ahmed P, Borchetia S, Kalita MC, Handique AK, Das S (2013) Molecular analysis of drought tolerance in tea by cDNA-AFLP based transcript profiling. Mol Biotech 53:237–248CrossRefGoogle Scholar
  29. Hucka M, Finney A, Sauro HM, Bolouri H, Doyle JC, Kitano H, Arkin AP, Bornstein BJ, Bray D, Cornish-Bowden A (2003) The Systems Biology Markup Language (SBML): A medium for the representation and exchange of biochemical network models. Bioinformat 19:524–531CrossRefGoogle Scholar
  30. Ishida M, Kitao N, Mizuno K, Tanikawa N, Kato M (2009) Occurrence of theobromine synthase genes in purine alkaloid-free species of Camellia plants. Planta 229:559–568PubMedCrossRefGoogle Scholar
  31. Jiang C, Li Y, Fang W (2005) cDNA cloning and prokaryotic expression of β-glucosidase in tea plant [Camellia sinensis (L.) O. Kutze]. Chinese J Agril Biotech 2:107–111Google Scholar
  32. Jiang C, Wen Q, Chen Y, Xu LA, Huang MR (2011) Efficient extraction of RNA from various Camellia species rich in secondary metabolites for deep transcriptome sequencing and gene expression analysis. Afr J Biotech 10:16769–16773CrossRefGoogle Scholar
  33. Kato M, Kanehara T, Shimizu H, Suzuki Gillies FM, Crozier A, Ashihara H (1996) Caffeine biosynthesis in young leaves of Camellia sinensis: in vitro studies on N-methyltransferase activity involved in the conversion of xanthosine to caffeine. Physiol Plant 98:629–636CrossRefGoogle Scholar
  34. Kato M, Mizuno K, Crozier A, Fujimura T, Ashihara A (2000) Caffeine synthase gene from tea leaves. Nat 406:956–957CrossRefGoogle Scholar
  35. Kato M, Mizuno K, Fujimura T, Iwama M, Irie M, Crozier A, Ashihara A (1999) Purification and characterization of caffeine synthase from tea leaves. Plant Physiol 120:579–586PubMedCentralPubMedCrossRefGoogle Scholar
  36. Kirita M, Honma D, Tanaka Y, Usui S, Shoji T, Sami M, Yokota T, Tagashira M, Muranaka A, Uchiyama M, Kanda T, Maeda-Yamamoto M (2010) Cloning of a novel O-methyltransferase from Camellia sinensis and synthesis of o-methylated EGCG and evaluation of their bioactivity. J Agric Food Chem 58:7196–7201PubMedCrossRefGoogle Scholar
  37. Krishnaraj T, Gajjeraman P, Palanisamy S, Chandrabose SRS, Mandal AKA (2011) Identification of differentially expressed genes in dormant (banjhi) bud of tea (Camellia sinensis (L.) O. Kuntze) using subtractive hybridization approach. Plant Physiol Biochem 49:565–571PubMedCrossRefGoogle Scholar
  38. Ku KM, Choi JN, Kim J, Kim JK, Yoo LG, Lee SJ, Hong YS, Lee CH (2010) Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.). J Agric Food Chem 58:418–426PubMedCrossRefGoogle Scholar
  39. Lee JE, Lee BJ, Chung JO, Hwang JA, Lee SJ, Lee CH, Hong YS (2010) Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: A 1H NMR-based metabolomics study. J Agri Food Chem 58:10582–10589CrossRefGoogle Scholar
  40. Lee JE, Lee BJ, Hwang JA, Ko KS, Chung JO, Kim EH, Lee SJ, Hong YS (2011) Metabolic dependence of green tea on plucking positions revisited: A metabolomic study. J Agri Food Chem 59:10579–10585CrossRefGoogle Scholar
  41. Li YH, Jiang CJ, Wan XC (2004a) Study on the expression of caffeine synthase gene mRNA in tea plant. J Tea Sci 24:23–28Google Scholar
  42. Li YH, Jiang CJ, Yang SL, Yu YB (2004b) β-glucosidase cDNA cloning in the tea (Camellia sinensis) and its prokaryotic expression. J Agri Biotech 12:625–629Google Scholar
  43. Li Y, Gu W, Ye S (2007) Expression and location of caffeine synthase in tea plants. Russ J Plant Physi 54:698–701CrossRefGoogle Scholar
  44. Li J, Chen J, Zhang Z, Pan Y (2008) Proteome analysis of tea pollen (Camellia sinensis) under different storage conditions. J Agric Food Chem 56:7535–7544PubMedCrossRefGoogle Scholar
  45. Li Q, Huang J, Liu S, Li J, Yang X, Liu Y, Liu Z (2011) Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar. Proteome Sci 9:44–56PubMedCentralPubMedCrossRefGoogle Scholar
  46. Liu Q, Tan X-F, Hu X-Y, Tian X-M (2010) Cloning and sequence analysis of a 14–3-3 protein gene from Camellia oleifera Acta Agri Univer 5:13–17Google Scholar
  47. Li XW, Liu HJ, Xie SX, Yuan HY (2013) Isolation and characterization of two genes of the early light-induced proteins of Camellia sinensis. Photosynthe 51:305–311CrossRefGoogle Scholar
  48. Li XW, Feng ZG, Yang HM, Zhu XP, Liu J, Yuan HY (2010) A novel cold-regulated gene from Camellia sinensis, CsCOR1, enhances salt- and dehydration-tolerance in tobacco. Biochem Biophys Res Commun 394:354–359PubMedCrossRefGoogle Scholar
  49. Lin GZ, Lian YJ, Ryu JH, Sung MK, Park JS, Park HJ, Park BK, Shin JS, Lee MS, Cheon CL (2007) Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli. Protein Exp Puri 55:287–292CrossRefGoogle Scholar
  50. Liu S, Han B (2010) Differential expression pattern of an acidic 9/13-lipoxygenase in flower opening and senescence and in leaf response to phloem feeders in the tea plant. BMC Plant Biol 10:228–238PubMedCentralPubMedCrossRefGoogle Scholar
  51. Marshall A, Gollapudi S, de Silva JD, Hodgman C (2007) A systems biology approach to modelling tea (Camellia sinensis). BMC Sys Biol 1(Suppl. 1):13CrossRefGoogle Scholar
  52. Ma C, Qiao X, Chen L (2010) Cloning and expression analysis of leucoanthocyantin reducase gene of tea plant (Camellia sinensis). J Tea Sci. doi:CNKI:SUN:CYKK.0.2010–01-006Google Scholar
  53. Ma C-L, Chen L, Wang X-C, Jin J-Q, Ma J-Q, Yao M-Z, Wang Z-L (2012) Differential expression analysis of different albescent stages of ‘Anji Baicha’ (Camellia sinensis (L.) O. Kuntze) using cDNA microarray. Scientia Hort 148:246–254CrossRefGoogle Scholar
  54. Matsumoto S, Takeuchi A, Hayatsu M (1994a) Chalcone synthase from Camellia sinensis: isolation of the cDNAs and the organ-specific and sugar-responsive expression of the genes. Plant Cell Physiol 35:1011–1018Google Scholar
  55. Matsumoto S, Takeuchi A, Hayatsu M (1994b) Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theor Appl Genet 89:671–675CrossRefGoogle Scholar
  56. Mizutani M, Nakanishi H, Ema J, Ma SJ, Noguchi E, Inohara-Ochiai M, Fukuchi-Mizutani M, Nakao M, Sakata K (2002) Cloning of β-primeverosidase from tea leaves, a key enzyme in tea aroma formation. Plant Physiol 130:2164–2176PubMedCentralPubMedCrossRefGoogle Scholar
  57. Mohanpuria P, Kumar V, Joshi R, Gulati A, Ahuja PS, Yadav SK (2009) Caffeine biosynthesis and degradation in tea (Camellia sinensis (L.) O. Kuntze) is under developmental and seasonal regulation. Mol Biotech 43(2):104–111CrossRefGoogle Scholar
  58. Mondal TK (2013) Application of highthroughput DNA sequencing for Plant Science research. In: Pullaiah T (ed) Abiotic Stress and Biotechnology. Abiotic stress and Biotechnology Regency Publications, New Delhi, pp 57–74Google Scholar
  59. Mondal TK, Rana MK (2013) High throughput DNA sequencing and its implication in plant science research. Everyman Sci 1:15–25Google Scholar
  60. Mondal TK, Sutoh K (2013) Application of Next-Generation Sequencing for Abiotic Stress Tolerance. In: Barh D, Zambare V, Azevedo V (eds) The world of omics and applications in plant and agricultural sciences. CRC Press, USA, pp. 383–402Google Scholar
  61. Mukhopadhaya M M, Sarkar B B, Mondal TK (2013) Omics advances of tea (Calliame sinensis). In: Omics Applications in crop science (Ed Bhar D) CRC Pres New York pp 439–466Google Scholar
  62. Murayama Y, Uematsu K, Koyama H, Morita A (2007) Detection of aluminium responsive genes in tea plant by hybridization with Arabidopsis thaliana cDNA. The 3rd Int Conf on O-Cha tea culture and science (ICOS) Shizuoka, Japan, 29 Feb 2008Google Scholar
  63. Muoki RC, Paul A, Kumar S (2012) A shared response of thaumatin like protein, chitinase, and late embryogenesis abundant protein3 to environmental stresses in tea [Camellia sinensis (L.) O. Kuntze]. Funct Integr Genom 12:565–571CrossRefGoogle Scholar
  64. Mueller LA, Zhang P, Rhee SY (2003) AraCyc: a biochemical pathway database for Arabidopsis. Plant Physiol 132:453–460PubMedCentralPubMedCrossRefGoogle Scholar
  65. Pan L-L, Wang Y, Hu J-H, Ding Z-T, Li C (2013) Analysis of codon use features of stearoyl-acyl carrier protein desaturase gene in Camellia sinensis. J Theor Biol 334:80–86PubMedCrossRefGoogle Scholar
  66. Pang Y, Abeysinghe ISB, He J, He X, Huhman D, Mewan KM, Sumner LW, Yun J, Dixon RA (2013) Functional characterization of proanthocyanidin pathway enzymes from tea (Camellia sinensis) and their application for metabolic engineering. Plant Physiol 161:1103–1116PubMedCentralPubMedCrossRefGoogle Scholar
  67. Park JS, Kim JB, Hahn BS, Kim KH, Ha SH, Kim JB, Kim YH (2004) EST analysis of genes involved in secondary metabolism in Camellia sinensis (tea), using suppression subtractive hybridization. Plant Sci 166:953–961CrossRefGoogle Scholar
  68. Paul A, Kumar S (2011) Responses to winter dormancy, temperature, and plant hormones share gene networks. Funct Integr Genom 11:659–664CrossRefGoogle Scholar
  69. Paul A, Kumar S (2013) Dehydrin2 is a stress-inducible, whereas Dehydrin1 is constitutively expressed but up-regulated gene under varied cues in tea [Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep 40:3859–3863PubMedCrossRefGoogle Scholar
  70. Paul A, Lal L, Ahuja PS, Kumar S (2012a) Alpha-tubulin (CsTUA) up-regulated during winter dormancyis a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep 39:3485–3490CrossRefGoogle Scholar
  71. Paul A, Muoki RC, Singh K, Kumar S (2012b) CsNAM-like protein encodes a nuclear localized protein and responds to varied cues in tea [Camellia sinensis (L.) O. Kuntze]. Gene 502:69–74CrossRefGoogle Scholar
  72. Phukon M, Namdev R, Deka D, Modi SK, Sen P (2012) Construction of cDNA library and preliminary analysis of expressed sequence tags from tea plant [Camellia sinensis (L) O. Kuntze]. Gene 506:202–206PubMedCrossRefGoogle Scholar
  73. Prabu GR, Mandal AKA (2010) Computational identification of miRNAs and their target genes from expressed sequence tags of tea (Camellia sinensis). Genom Proteom Bioinform 8:113–121CrossRefGoogle Scholar
  74. Prabu GR, Thirugnanasambantham K, Mandal AKA, Saravanan A (2012) Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea. Biol Planta 56(1):140–144CrossRefGoogle Scholar
  75. Punyasiri PA, Abeysinghe IS, Kumar V, Treutter D, Duy D, Gosch C, Martens S, Forkmann G, Fischer TC (2004) Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways. Arch Biochem Biophys 431:22–30PubMedCrossRefGoogle Scholar
  76. Qiao J, Zhang YT, Zhu XP (2011) Identification of genes up-regulated by ectropic obliqua feeding in tea seedlings. Acta Hort Sinica 38:783–789Google Scholar
  77. Rana NK, Mohanpuria P, Yadav SK (2008a) Cloning and characterization of a cytosolic glutamine synthetase from Camellia sinensis (L.) O. Kuntze that is upregulated by ABA, SA, and H2O2. Mol Biotech 39:49–56CrossRefGoogle Scholar
  78. Rana NK, Mohanpuria P, Yadav SK (2008b) Expression of tea cytosolic glutamine synthetase is tissue specific and induced by cadmium and salt stress. Biol Planta 52:361–364CrossRefGoogle Scholar
  79. Rana NK, Mohanpuria P, Kumar V, Yadav SK (2010) A CsGS is regulated at transcriptional level during developmental stages and nitrogen utilization in Camellia sinensis (L.) O. Kuntze. Mol Biol Rep 37:703–710PubMedCrossRefGoogle Scholar
  80. Rani A, Singh K, Ahuja PS, Kumar S (2012) Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze]. Gene 495:205–210PubMedCrossRefGoogle Scholar
  81. Rani A, Singh K, Sood P, Kumar S, Ahuja PS (2009) p-Coumarate: CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]. Funct Integr Genom 9:271–275CrossRefGoogle Scholar
  82. Roy SC, Chakraborty BN (2009) Cloning and sequencing of chitinase gene specific PCR amplified DNA fragment from tea plant (Camellia sinensis) and analyzed the nucleotide sequence using bioinformatics algorithms. Can J Pure Appl Sci 3:795–800Google Scholar
  83. Sahu J, Sarmah R, Dehury B, Sarma K, Sahoo S, Sahu M, Barooah M, Modi MK, Sen P (2012) Mining for SSRs and FDMs from expressed sequence tags of Camellia sinensis. Bioinforma 8:260–266CrossRefGoogle Scholar
  84. Senthilkumar P, Thirugnanasambantham K, Mandal AKA (2012) Suppressive subtractive hybridization approach revealed differential expression of hypersensitive response and reactive oxygen species production genes in tea (Camellia sinensis (L.) O. Kuntze) leaves during Pestalotiopsis thea Infection. Appl Biochem Biotech 168:1917–1927CrossRefGoogle Scholar
  85. Sharma P, Kumar S (2005) Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze]. J Biosci 30:231–235PubMedCrossRefGoogle Scholar
  86. Shi Hï¼WangYï¼YangL-Cï¼DZ-T (2012) Analysis of codon bias of the cold regulated transcription factor ICE1 in tea plant. Acta Hort Sin 39:1341–1352Google Scholar
  87. Shi CY, Yang H, Wei CL, Yu O, Zhang ZZ, Jiang CJ, Sun J, Li YY, Chen Q, Xia T, Wan XC (2011) Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compounds. BMC Genom 12:131–150CrossRefGoogle Scholar
  88. Singh K, Kumar S, Ahuja PS (2009d) Differential expression of histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue. Mol Biol Rep 36:537–542CrossRefGoogle Scholar
  89. Singh K, Kumar S, Rani A, Gulati A, Ahuja PS (2009c) Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Funct Integr Genom 9:125–134CrossRefGoogle Scholar
  90. Singh K, Kumar S, Yadav SK, Ahuja PS (2009a) Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze]. Plant Biotech Rep 3:95–101CrossRefGoogle Scholar
  91. Singh K, Paul A, Kumar S, Ahuja PS (2009e) Cloning and differential expression of QM like protein homologue from tea [Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep 36:921–927CrossRefGoogle Scholar
  92. Singh K, Rani A, Kumar S, Sood P, Mahajan M, Yadav SK, Singh B, Ahuja PS (2008) An early gene of the flavonoid pathway, flavanone 3-hydroxylase, exhibits a positive relationship with the concentration of catechins in tea (Camellia sinensis). Tree Physiol 28:1349–1356PubMedCrossRefGoogle Scholar
  93. Singh K, Rani A, Paul A, Dutt S, Joshi R, Gulati A, Ahuja PS, Kumar S (2009b) Differential display mediated cloning of anthocyanidin reductase gene from tea (Camellia sinensis) and its relationship with the concentration of epicatechins. Tree Physiol 29:837–846CrossRefGoogle Scholar
  94. Singh K, Raizada J, Bhardwaj P, Ghawana S, Rani A, Singh H, Kaul K, Kumar S (2004) 26S rRNA-based internal control gene primer pair for reverse transcription-polymerase chain reaction-based quantitative expression studies in diverse plant species. Anal Biochem 335:330–333PubMedCrossRefGoogle Scholar
  95. Sugiyama T, Sadzuka Y (2003) Theanine and glutamate transporter inhibitors enhance the antitumor efficacy of chemotherapeutic agents. Biochem Biophys Acta 1653:47–59PubMedGoogle Scholar
  96. Sugiyama T, Sadzuka Y (2004) Theanine, a specific glutamate derivative in green tea, reduces the adverse reactions of doxorubicin by changing the glutathione level. Canc Lett 212:177–184CrossRefGoogle Scholar
  97. Suzuki T (1972) The participation of S-adenosylmethionine in the biosynthesis of caffeine in the tea plants. FEBS Lett 24:18–20CrossRefGoogle Scholar
  98. SuXia X, Yuan Z, KaiLin G, Lei W, HongYu Y (2009) Cloning and expression analysis of a flavonol synthase gene from Camellia sinensis. Plant Physiol Commun 45:1093–1097Google Scholar
  99. Takeo T (1981) Production of linalool and geraniol by hydrolytic breakdown of bound forms in disrupted tea shoots. Phytochem 120:2145–2147CrossRefGoogle Scholar
  100. Takechi A, Matsumoto S (2003) Effect of light on gene expression of catechin biosynthetic expression in tea (Camellia sinensis) leaves. Tea Res J 96:27–32CrossRefGoogle Scholar
  101. Takeuchi A, Matsumoto S, Hayatsu M (1994a) Chalcone synthase from Camellia sinensis isolation of the cDNAs and the organ-specific and sugar-responsive expression of the genes. Plant Cell Physiol 35:1011–1018Google Scholar
  102. Takeuchi A, Matsumoto S, Hayatsu M (1994b) Amplification of β-tubulin cDNA from Camellia sinensis by PCR. Bull Natl Res Inst Veg Ornam Plants Tea 7:13–20Google Scholar
  103. Taniguchi F, Tanaka J (2004) Characterization of genes for ammonium assimilation in Camellia sinensis. Proc of 2004 Int Con on O-Cha culture and Sci. Shizuaka, Japan, 217–218Google Scholar
  104. Taniguchi F, Fukuoka H, Tanaka J (2012) Expressed sequence tags from organ-specific cDNA libraries of tea (Camellia sinensis) and polymorphisms and transferability of EST-SSRs across Camellia species. Breed Sci 62:186–195PubMedCentralPubMedCrossRefGoogle Scholar
  105. Thirugnanasambantham K, Prabu G, Palanisamy S, Subhas Chandrabose SR, Mandal AKA (2013) Analysis of dormant bud (Banjhi) specific transcriptome of tea (Camellia sinensis (L.) O. Kuntze) from cDNA library revealed dormancy-related genes. Appl Biochem Biotechnol 169:1405–1417PubMedCrossRefGoogle Scholar
  106. Tomimoto Y, Ikehashi I, Kakeda K, Kowyama Y (1999) A pistal specific PR-1 like protein in Camellia, its expression, sequence and genealogical position. Breed Sci 49:97–104CrossRefGoogle Scholar
  107. Venkatesh P, Jaiprakash M, Prasad P, Pillai B, Sadhale PP, Sinkar VP (2006) Flavonoid biosynthesis in tea (Camellia sinensis).–6-2.pdf
  108. Waheed A, Hamid FS, Shah AH, Ahmad H, Khalid A, Abbasi FM, Ahmad N, Aslam S, Sarwar S (2012) Response of different tea (Camellia sinensis L.) clones against drought stress. J Master Environ Sci 3:395–410Google Scholar
  109. Wang CX, Li YY, Jiang CJ, Yu YB (2005) Molecular cloning and sequence analysis on cDNA of cystatin gene from tea leaves. J Tea Sci 25:177–182Google Scholar
  110. Wang L, Li X, Zhao Q, Jing S, Chen S, Yuan H (2009) Identification of genes induced in response to low-temperature treatment in tea leaves. Plant Mol Biol Rep 27:257–265CrossRefGoogle Scholar
  111. Wang X-C,Yang YJ,Chen L,Ma CL,Yao MZ (2010) Construction and preliminary analysis of the suppression subtractive hybridization cDNA libraries between dormant and sprouting buds of tea plant (Camellia sinensis). J Tea Sci 30:129–135Google Scholar
  112. Wang P, Su R, Zheng J, Cheng S, Zhu G (2011a) Molecular cloning and phylogenetic analysis of a novel BURP domain-containing gene from Camellia sinensis. Afr J Biotech 10:15470–15476Google Scholar
  113. Wang X, Ma C, Yang C, Yao M, Jin J (2011b) Cloning and expression analysis of auxin-repressed protein gene CsARP1 in tea plant (Camellia sinensis). J Nuclear Agric Sci. doi:CNKI:SUN:HNXB.0.2011–05-014Google Scholar
  114. Wang X, Ma C, Yang Y, Jin J, Ma J, Cao H (2012a) cDNA cloning and expression analysis of cyclindependent kinase (CsCDK) gene in tea plant. Acta Hort Sinica 39:333–342Google Scholar
  115. Wang X, Yang Y, Ma C, Jin J, Ma J, Cao H (2011c) Cloning and expression analysis of cyclin gene (CsCYC1) of tea plant. Acta Botanica Boreali-Occidentalia Sinica 31:2365–2372Google Scholar
  116. Wang Y, Jiang C, Li Y, Wei C, Deng W (2012b) CsICE1 and CsCBF1:two transcription factors involved in cold responses in Camellia sinensis. Plant Cell Rep 31:27–34CrossRefGoogle Scholar
  117. Wang YS, Gao LP, Shan Y, Liu YJ, Tian YW, Xia T (2012) Influence of shade on flavonoid biosynthesis in tea (Camellia sinensis (L.) O. Kuntze). Scientia Hort 141:7–16CrossRefGoogle Scholar
  118. Wang YS, Gao LP, Wang ZR, Liu YJ, Sun M, Zeng W, Yuan H (2012c) Light induced expression of genes involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis (L.) O.Kuntze). Sci Hort 133:72–83CrossRefGoogle Scholar
  119. Wang P, Song P, Li X, Su R, Wang H, Zhu G (2012d) Study on soluble expression of glutamate dehydrogenase from tea plant in Escherichia coli using fusion tags. Afr J Biotech 11:6241–6250CrossRefGoogle Scholar
  120. Wang X-C, Zhao Q-Y, Ma C-L, Zhang Z-H, Cao H-L, Kong Y-M, Yue C, Hao X-Y, Chen L, Ma J-Q, Jin J-Q, Li X, Yang Y-J (2013) Global transcriptome profiles of Camellia sinensis during cold acclimation. BMC Genomics 2013 14:415PubMedCentralPubMedCrossRefGoogle Scholar
  121. Wei CL, Jiang CJ, Tao HZ, Wan XC (2003) Cloning and bioinformatics analysis of sequence signature of violaxanthin de-epoxidase cDNA in tea plant (Camellia sinensis (L.) O. Kuntze). J Nanj Agri Univ 26:14–19Google Scholar
  122. Wei CL, Jiang CJ, Tao HZ, Wan XC (2004) Site-directed mutation of violaxanthin de-epoxidase from tea plant (Camellia sinensis) in vitro and expression of bioactivity assay of the mutants. Chinese J Biochem Mol Biol 20:73–78Google Scholar
  123. Wei K, Wang L, Cheng H, Zhang C, Ma C, Zhang L, Gong W, Wu L (2013) Identification of genes involved in indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.) by suppression subtractive hybridization. Gene 514:91–98PubMedCrossRefGoogle Scholar
  124. Wu YL, Pan LP, Yu SL, Li HH (2010) Cloning, microbial expression and structure-activity relationship of polyphenol oxidases from Camellia sinensis. J Biotech 145:66–72CrossRefGoogle Scholar
  125. Wu H, Chen D, Li J, Yu B, Qiao X, Huang H, He Y (2013) De novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea (Camellia sinensis). Plant Mol Biol Rep 31:524–538CrossRefGoogle Scholar
  126. Yadav SK (2009) Computational structural analysis and kinetic studies of a cytosolic glutamine synthetase from Camellia sinensis (L.) O. Kuntze. Protein J 28:428–434PubMedCrossRefGoogle Scholar
  127. Yang H, Xie S, Wang L, Jing S, Zhu X, Li X, Zeng W, Yuan H (2011) Identification of up-regulated genes in tea leaves under mild infestation of green leafhopper. Sci Hort 130:476–448CrossRefGoogle Scholar
  128. Yang D, Liu Y, Suna M, Zhao L, Wang Y, Chen X, Wei C, Gao L, Xia T (2012a) Differential gene expression in tea (Camellia sinensis L. ) calli with different morphologies and catechin contents. J Plant Physiol 169:163–175CrossRefGoogle Scholar
  129. Yang YJ, Wang XC, Ma CL (2012b) Cloning and bioinformatics analysis of full-length cDNA of actin gene (CsActin1) from tea plant (Camellia sinensis (L.) O. Kuntze). Bull Bot Res 32:69–76Google Scholar
  130. Yoshida K, Homma T (2005) Isolation of wound/pathogen inducible cDNA from tea by mRNA differential display. Inter Tea Symp-2005 62–65 (Hanzou, China)Google Scholar
  131. Ye Y, Jiang CJ, Zhu L, Yu M, Wang ZX, Deng WW, Wei CL (2009) Cloning and sequencing of a full length cDNA encoding the RuBPCase small subunit (RbcS) in tea (Camellia sinensis). Agric Sci China 8:161–166CrossRefGoogle Scholar
  132. Zhao D, Liu ZS, Xi B (2001) Cloning and alignment of polyphenols oxidase cDNA of tea plant. J Tea Sci 21:94–98Google Scholar
  133. Zhao LP, Chen L, Wang XC, Yao MZ (2006a) Quantitative detection of β-glucosidase and β-primeveroside gene expressions in different leaves of tea plant (Camellia sinensis) by real-time PCR analysis. J Tea Sci 26:11–16Google Scholar
  134. Zhao LP, Gao QK, Chen L, Wang XC, Yao MZ (2006b) Development and preliminary application of cDNA microarray of tea plant (Camellia sinensis). J Tea Sci 5:3–7Google Scholar
  135. Zhao L, Gao L, Wang H, Chen X, Wang Y, Yang H, Wei C, Wan X, Xia T (2012) The R2R3-MYB, bHLH, WD40, and related transcription factors in flavonoid biosynthesis. Funct Integr Genom. 13:75–98Google Scholar
  136. Zhang YL, Zhao LP, MA CL, Chen L (2007) Molecular identification, bioinformatic analysis and prokaryotic expression of the cyclophilin gene full length cDNA from tea plant (Camellia sinensis). J Tea Sci 27:120–126Google Scholar
  137. Zhang YL, Qiao XY, Chen L (2008a) Full-length cDNA cloning and bioinformatic analysis of ACC synthase gene from the tea plant (Camellia sinensis). J Tea Sci 28:235–241Google Scholar
  138. Zhang YL, Qiao XY, Chen L (2008b) Molecular cloning and expression analysis of ACC oxidase gene full-length cDNA from the tea plant (Camellia sinensis). J Tea Sci 28:459–466Google Scholar
  139. Zhang X, Liu Y, Gao K, Zhao L, Liu L, Wang Y, Sun M, Gao L, Xia T (2012) Characterization of anthocyanidin reductase from Shuchazao green tea. J Sci Food Agric 92:1533–1539PubMedCrossRefGoogle Scholar
  140. Zhu L, Deng WW, Ye AH, Yu M, Wang ZX, Jiang CJ (2008a) Cloning of two cDNAs encoding a family of ATP sulfurylase from Camellia sinensis related to selenium or sulfur metabolism and functional expression in Escherichia coli. Plant Physiol Biochem 46:731–738CrossRefGoogle Scholar
  141. Zhu L, Jiang CJ, Deng WW, Gao X, Wang RJ, Wan XC (2008b) Cloning and expression of selenocysteine methyltransferase cDNA from Camellia sinensis. Acta Physiol Plnt 30:167–174CrossRefGoogle Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Division of Genomic ResourcesNational Bureau of Plant Genetic ResourcesDelhiIndia

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