Molecular Markers

  • Tapan Kumar Mondal


Among the several factors described in chapter 2, progress of tea breeding had also been slowed down due to the lack of reliable selection criteria. Thus various morpho-biochemical, digital markers such as artificial neural network, metallic markers, isozymes, are described. However, it had been seen that they had marginally improved the efficacy of selection for desired agronomic traits in tea. This was mainly due to the fact that most of these markers defined so far were influenced greatly by the environmental factors and hence showed a continuous variation with a high degree of plasticity. Recently, development of the molecular biology had resulted in alternative DNA-based markers for crop improvement of tea. These markers can assist the process of traditional breeding with several efficacies. The greatest advantages of molecular markers are: (1) free from the environmental influences and (2) detection of polymorphisms at an early stage of growth. The different DNA besed markers, which have been employed for varietal improvement of tea and its wild relatives, are reviewed in this chapter.


Amplify Fragment Length Polymorphism Amplify Fragment Length Polymorphism Marker Cleave Amplify Polymorphic Sequence Cleave Amplify Polymorphic Sequence Marker Learn Vector Quantization Neural Network 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Ackerman WL (1971) Genetic and cytological studies with Camellia and related genera. Technical Bull No 1427. USDA/U.S. Govt. Print Office, Washington DC, p 115Google Scholar
  2. Afridi SG, Ahmad H, Alam M, Khan IA, Hassan M (2011) DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers. Afr J Biotech 10:15477–15482Google Scholar
  3. Alcázar A, Ballesteros O, Jurado JM, Pablos F, Martín MJ, Vilches JL, Navalón A (2007) Differentiation of green, white, black, Oolong, and Pu-erh teas according to their free amino acids content. J Agric Food Chem 55:5960–5965PubMedGoogle Scholar
  4. Anderson S (1994) Isozyme analysis to differentiate between tea clones. Inligtingsbulletin Institut vir Tropiese en Subtropiese Gewasse 266:15Google Scholar
  5. Ariyaratne PNK, Mewan KM, Goonetilleke WASNST, Attanayake DPSTG (2009) Study of genetic relationships of tea (Camellia sinensis (L.) O. Kuntze) using SSR markers and pedigree analysis. Proc 9th Agr Res Symp 1:293–298Google Scholar
  6. Balasaravanan T, Pius PK, Kumar RR (2002) Assessment of genetic fidelity among the in vitro propagated culture lines of Camellia sinensis (L.) O kuntze using RAPD markers. In: Proceedings of the 15th Plantation Crops Symposium PLACROSYM XV Mysore, 10–13 Dec, pp 181–184Google Scholar
  7. Balasaravanan T, Pius PK, Kumar RR, Muraleedharan N, Shasany AK (2003) Genetic diversity among south Indian tea germplasm (Camellia sinensis, C. assamica and C. assamica spp. Lasiocalyx) using AFLP markers. Plant Sci 165:365–372Google Scholar
  8. Bali S, Raina SN, Bhat V, Aggarwal RK, Goel S (2013) Development of a set of genomic microsatellite markers in tea (Camellia L.) (Camelliaceae). Mol Breeding 32:735–741. doi: 10.1007/s11032-013-9902-4Google Scholar
  9. Banerjee B (1987) Can leaf aspect affect herbivory? A case study with tea. Ecol 68:839–834Google Scholar
  10. Banerjee B (1992) Botanical classification of tea. In: Wilson KC, Clifford MN (eds) Tea cultivation to consumption. Chapman and Hall, London, pp 25–51Google Scholar
  11. Barua DN (1958) Leaf sclereids in the taxonomy of the Thea camellias. I. Wilson’s and related Camellias. Phytomorphology 8:257–264Google Scholar
  12. Barua DN, Dutta AC (1959) Leaf sclereids in taxonomy of Thea camellias II. Camellia sinensis L. Phytomorphology 9:372–382Google Scholar
  13. Barua DN, Dutta KN (1971) Distribution of shoots on the plucking surface of a tea bush and its relation to spacing. Two Bud 18:8–11Google Scholar
  14. Barua PK (1963) Classification of tea plant. Two Bud 10:3–11Google Scholar
  15. Bedrook JR, Kolodner R (1979) The structure of choloroplast DNA. Annu Rev Plant Physiol 30:593–620Google Scholar
  16. Bera B, Saikia H (2002) Randomly amplified polymorphic DNA (RAPD) marker analysis in tea (Camellia sinensis L) generative clones. In Proceedings of the 15th plantation crops symposium PLACROSYM XV Mysore 10–13 Dec, pp 235–238Google Scholar
  17. Bezbaruah HP (1971) Cytological investigation in the family theaceae—I. Chromosome numbers in some Camellia species and allied genera. Caryologia 24:421–426Google Scholar
  18. Borchetia S, Das SC, Handique PJ, Das S (2009) High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea (Camellia spp.). Sci Hort 120:544–550Google Scholar
  19. Borse BB, Rao LJM, Nagalakshmi S, Krishnamurthy N (2002) Fingerprint of black teas from India: identification of the regio-specific characteristics. Food Chem 79:419–424Google Scholar
  20. Borthakur S, Mondal TK, Borthakur A, Deka PC (1995) Variation in peroxidase and esterase isoenzymes in tea leaves. Two Bud 42:20–23Google Scholar
  21. Borthakur S, Mondal TK, Parveen SS, Guha A, Sen P, Borthakur A, Deka PC (1998) Isolation of chloroplast DNA from tea, Camellia sp. Ind J Exp Biol 36:1165–1167Google Scholar
  22. Caser M, Torello Marinoni D, Scariot V (2010) Microsatellite-based genetic relationships in the genus Camellia: potential for improving cultivars. Genome 53:384–99PubMedGoogle Scholar
  23. Chang HT (1981) A taxonomy of the genus Camellia. Acta Scientiarum Naturalium Universitatis Sunyatseni, Monograph series 1:1–180Google Scholar
  24. Chen C (1996) Analysis on the isozymes of tea plants F1 hybrids. J Tea Sci 16:31–33Google Scholar
  25. Chen J, Wang PS, Xia YM, Xu M, Pei SJ (2005a) Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Genetic Res Crop Evol 52:41–52Google Scholar
  26. Chen L, Chen D, Gao Q, Yang Y, Yu F (1997a) Isolation and appraisal of genomic DNA from tea plant (Camellia sinensis (L.) O. Kuntze. J Tea Sci 17:177–181Google Scholar
  27. Chen L, Gao Q, Yang Y, Yu F, Chen D (1998a) Optimum amplification procedure and reaction system for RAPD analysis of tea plant (Cameliia sinensis (L.) O. Kuntze. J Tea Sci 18:16–20Google Scholar
  28. Chen L, Gao QK, Chen DM, Xu CJ (2005b) The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Biodiver Conser 14:1433–1444Google Scholar
  29. Chen L, Qiqing T, Qikang G, Jilin S, Fulian Y (1997b) Observation on pollen morphology of 8 species and 1 variety in genus Camellia. J Tea Sci 17:183–188Google Scholar
  30. Chen L, Tong Q, Zhuang W (1992) Studies on pollen morphology and fuzzy clustering analysis of tea. Acta Agri Uni Zhejiang (Chinese) 18:29–36Google Scholar
  31. Chen L, Wang PS, Yamaguchi S (2002a) Discrimination of wild tea germplasm resources (Camellia sp.) using RAPD markers. Agri Sci China 1:1105–1110Google Scholar
  32. Chen L, Wang PS, Yamaguchi S (2002b) Discrimination of wild tea germplasm resources (Camellia sp.) using RAPD markers. Agri Sci China 1:1105–1110Google Scholar
  33. Chen L, Zhou Z (2005) Variations of main quality components of tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China National Germplasm Tea Repository. Plant Food Hum Nut 60:31–35Google Scholar
  34. Chen L, Yamaguchi L (2002) Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis. J Hort Sci Bio 77:729–732Google Scholar
  35. Chen L, Yamaguchi S (2005) RAPD markers for discriminating tea germplasms at the inter-specific level in China. Plant Breed 124:404–409Google Scholar
  36. Chen L, Yamaguchi S, Wang PS, Xu M, Song WX, Tong QQ (2002b) Genetic polymorphism and molecular phylogeny analysis of section Thea based on RAPD markers. J Tea Sci 22:19–24Google Scholar
  37. Chen L, Yang Y, Yu F, Gao Q, Chen D (1998b) Genetic diversity of 15 tea (Camellia sisnensis (L.) O.Kuntze) cultivars using RAPD markers. J Tea Sci 18:21–27Google Scholar
  38. Chen L, Yu F, Yang Y, Gao Q, Chen D, Xu C (1999) A study on genetic stability of excellent germplasm (Camellia sisnensis (L.) O. Kuntze) using RAPD markers. J Tea Sci 19:13–16Google Scholar
  39. Chen Q, Zhao J, Liu M, Cai J (2008) Nondestructive identification of tea (Camellia sinensis L.) varieties using FT-NIR spectroscopy and pattern recognition. Czech J Food Sci 26:360–367Google Scholar
  40. Chen Y, Yu M, Xu J, Chen X, Shi J (2009) Differentiation of eight tea (Camellia sinensis) cultivars in China by elemental fingerprint of their leaves. J Sci Food Agric 89:2350–2355Google Scholar
  41. Chengyin L, Weihua L, Mingjun (1992) Relationship between the evolutionary relatives and the variation of esterase isozymes in tea plant. J Tea Sci 12:15–20Google Scholar
  42. Chung MY, Epperson BK, Chung MG (2003) Genetic structure of age classes in Camellia japonica (THEACEAE). Evol 57:62–73Google Scholar
  43. Clark JY, Warwick K (1998) Artificial keys for botanical identification using a multilayer perception neural network (MLP). Artif Intell Rev 12:95–115Google Scholar
  44. Cummings MP, Otto SP, Wakeley J (1995) Sampling properties of DNA sequence data in phylogenetic analysis. Mol Biol Evol 12:814–822PubMedGoogle Scholar
  45. Damasco OP, Godwin ID, Smith MK, Adkins SW (1996) Gibberellic acid detection of dwarf off-types in micropropagated Cavendish bananas. Aus J Expt Agric 36:237–241Google Scholar
  46. Das A, Mondal TK (2010) In silico analysis of miRNA and their targets in tea. Amer J Plant Sci 1:77–86Google Scholar
  47. Deng WW, Ogita S, Ashihara H (2010) Distribution and biosynthesis of theanine in Theaceae plants. Plant Physiol Biochem 48:70–72PubMedGoogle Scholar
  48. Dhiman B, Singh M (2003) Molecular detection of cashew husk (Anacardium occidentale) adulteration in market samples of dry tea (Camellia sinensis). Planta Med 69:882–884PubMedGoogle Scholar
  49. Eden T (1976) Tea. Longman, London, p 236Google Scholar
  50. Evans DA, Sharp WR (1986) Somaclonal and gametoclonal variation. In: Evans DA, Sharp WR, Ammirato PV (eds) Technique and Applications. Handbook of Plant Cell Culture, vol 42. Macmillan Publishing Company, New York, pp 97–132Google Scholar
  51. Fan K, Hong Y-C, Ding Z-T, Wang Y (2010) Analysis of genetic diversity among natural hybrid progenies of Camellia sinensis ‘Huangshanzhong’. Acta Hort Sinica 37:1357–1362Google Scholar
  52. Fang SW, Hua PR, Sheng WP, Mei X, Xing DH, Ping ZY, Hua LJ, Shao WF, Pang RH, Wang PS, Xu M, Duan HX, Zhang YP, Li JH (2003) RAPD analysis of tea trees in Yunnan. Sci Agrire Sinica 36:1582–1587Google Scholar
  53. Fang W, Cheng H, Duan Y, Jiang X, Li X (2012) Genetic diversity and relationship of clonal tea (Camellia sinensis) cultivars in China as revealed by SSR markers. Plant Sys Evol 298:469–483Google Scholar
  54. Ferguson JM, Grabe DF (1986) Identification of cultivars of perennial rye grass by SDS-PAGE of seed proteins. Crop Sci 26:170–176Google Scholar
  55. Fernández-Cáceres PL, Martín MJ, Pablos F, González AG (2001) Differentiation of tea (Camellia sinensis) varieties and their geographical origin according to their metal content. J Agric Food Chem 49:4775–4779PubMedGoogle Scholar
  56. Freeman SJ, West CJ, Lea V, Mayes S (2004) Isolation and characterization of highly polymorphic microsatellites in tea (Camellia sinensis). Mol Ecol Notes 4:324–326Google Scholar
  57. Fukushima E, Iwasa S, Endo N, Yoshinari T (1966) Cytogenetics studies in Camellia. I. Chromosome survey in some Camellia species. Jap J Hort 35:413–421Google Scholar
  58. George O, Adam M (2006). Investigation into the evolutionary origins of Theaceae and genus Camellia. Int Camellia J 38:78–89Google Scholar
  59. Ghosh-Hazra N (2001) Advances in selection and breeding of tea—a review. J Plant Crop 29:1–17Google Scholar
  60. Goonetilleke WASNST, Priyantha PGC, Mewan KM, Gunasekare MTK (2009) Assessment of genetic diversity of tea (Camellia sinensis L.O. Kuntze) as revealed by RAPD-PCR markers. J Nat Sci Found Sri Lanka 37:147–150Google Scholar
  61. Graybeal A (1998) Is it better to add taxa or characters to a difficult phylogenetic problem? Syst Biol 47:9–17PubMedGoogle Scholar
  62. Gu Z, Xiao H (2003) Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae). Plant Sci 164:279–285Google Scholar
  63. Gul S, Ahmad H, Khan IA, Alam M (2007) Assessment of diversity of tea genotypes through RAPD markers. Pak J Biol Sci 10:2609–2611PubMedGoogle Scholar
  64. Gulati A, Rajkumar S, Karthigeyan S, Sud RK, Vijayan D, Thomas J, Rajkumar R, Das SC, Tamuly P, Hazarika M, Ahuja PS (2009) Catechin and catechin fractions as biochemical markers to study the diversity of Indian tea (Camellia sinensis (L.) O. Kuntze) germplasm. Chem Biodivers 6:1042–1052PubMedGoogle Scholar
  65. Gunasekara MTK, Arachchige JDK, Mudalige AK, Peiris TUS (2001) Morphological diversity of tea (Camellia sinensis L.) genotypes in Sri Lanka. In: Proceedings of the 57th annual session of Sri Lanka Association for the Advancement of Science (SLAAS) Part I. Colombo, p 83Google Scholar
  66. Hackett CA, Wachira FN, Paul S, Powell W, Waugh R (2000) Construction of a genetic linkage map for Camellia sinensis (tea). Heredity (Edinb.) 85:346–355Google Scholar
  67. Hairong X, Qiqing T, Wanfanz Z (1987) Studies on the genetic tendency of tea plant hybrid generation using isozyme technique. In: Proceedings of the international symposium on tea quality and human health. China, 4–9 November 1987, p 21Google Scholar
  68. Hammerschlag FA (1992) Somaclonal variation. In: Hammerschlag FA, Lit RE (eds) Biotechnology of perennial fruit crops. CAB International, Wallingford, pp 35–55Google Scholar
  69. Hazarika M, Mahanta PK (1984) Composition changes in chlorophylls and carotenoids during the four flushes of tea in North-East India. J Sci Food Agri 35:298–303Google Scholar
  70. Hirai M, Kozaki I (1986) Isozymes of citrus leaves. In: Kitaura K, Akihama T, Kukimura H, Nakajima H, Horie M, Kozaki I (eds) Development of new technology for identification and classification of tree crops and ornamentals. Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries, Japan, pp 73–76Google Scholar
  71. Hou YJ, He Q, Li ZL, Li PW, Liang GL, Xu J (2007) ISSR applied to the germplasms identification of Camellia sinensis. Southwest China J Agri Sci 26:1272–1276Google Scholar
  72. Hu CY, Lee TC, Tsai HT, Tsai YZ, Lin SF (2013) Construction of an integrated genetic map based on maternal and paternal lineages of tea (Camellia sinensis). Euphytica 191(1):141–152. doi: 10.1007/s10681-013-0908-0Google Scholar
  73. Huang FP, Liang YR, Lu JL, Chen RB (2006a) Genetic mapping of first generation of backcross in tea by RAPD and ISSR markers. J Tea Sci 26:171–176Google Scholar
  74. Huang JA, Huang YH, Luo JW, Li JX, Gong ZH, Liu ZH (2007) Identification of single nucleotide polymorphism in polyphenol oxidase gene in tea plant (Camellia sinensis). J Hunan Agri Uni 33:454–458Google Scholar
  75. Huang JA, Li JX, Huang YH, Luo JW, Gong ZH, Liu ZH (2005). Construction of AFLP molecular markers linkage map in tea plant. J Tea Sci 25:7–15Google Scholar
  76. Huang JN, Li J, Huang Y, Luo J, Zong Z, Liu Z (2006b) Genetic diversity of tea [Camellia sinensis (L.) O. Kuntze] cultivars revealed by AFLP Analysis. Acta Hort Sinica 33:317–322Google Scholar
  77. Huelsenbeck JP (1995) Performance of phylogenetic methods in simulation. Syst Biol 44:17–48Google Scholar
  78. Hui LX, Lin LC, Peng SZ, Wu LJ, Wen SC, Hua GZ, Xuan C, Li XH, Liu CL, Shi ZP, Luo JW, Shen CW, Gong ZH, Chen X (2004) Analysis of genetic relationships among "Rucheng Baimao Cha" plants with RAPD method. J Tea Sci 24:33–36Google Scholar
  79. Hung CY, Wang KH, Huang CC, Gong X, Ge XJ, Chiang TY (2008) Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet 9:779–781Google Scholar
  80. Ikeda N, Kawada M, Takeda Y (1991) Isozymic analysis of Camellia sinensis and its interspecific hybrids. In: Proceedings of the international symposium of tea science, Shizouka, Japan, 26–28 Aug 1991, p 98Google Scholar
  81. IPGRI (1997) Descriptors for tea (Camellia sinensis). International Plant Genetic Resources Institute, RomeGoogle Scholar
  82. Isabel NL, Tremblay MM, Tremblay FM, Bousquet J (1993) RAPD as an aid to evaluate the genetic integrity of somatic embryogenesis derived population of Picea mariana (Mill) B.S.P. Theor Appl Genet 86:81–87PubMedGoogle Scholar
  83. Jeyaramraja PR, Jayakumar D, Kumar RR, Pius PK (2002) Peroxidase isozyme—a versatile tool for screening of drought tolerant clones. Newsl UPASI Res Found 12:1–7Google Scholar
  84. Jha TB, Sen SK (1992) Micropropagation of an elite Darjeeling tea clone. Plant Cell Rep 11:101–104Google Scholar
  85. Ji PZ, Li H, Gao LZ, Zhang J, Chen GZQ, Huang XQ (2011) ISSR diversity and genetic differentiation of ancient tea (Camellia sinensis var. assamica) plantations from China: implications for precious tea germplasm conservation. Pak J Bot 43:281–291Google Scholar
  86. Ji PZ, Zhang J, Wang PS, Huang XQ, Xu M, Tang YC, Liang MZ (2007) Genetic diversity of ancient tea plant in Yunnan province of Chian revealed by inter-simple sequence repeat (ISSR) polymerase chain reaction. J Tea Sci 27:271–279Google Scholar
  87. Jin JQ, Cui HR, Chen WY, Lu MZ, Yao YL, Xin Y, Gong XC (2006) Data mining for SSR in ESTs and development of EST-SSR marker in tea plant (Camellia sinensis). J Tea Sci 26:17–23Google Scholar
  88. Jin JQ, Cui HR, Gong XC, Chen WY, Xin Y (2007) Studies on tea plants (Camellia sinensis) germplasms using EST-SSR marker. Yi Chuan 29:103–108PubMedGoogle Scholar
  89. Jorge S, Pedroso MC, Neale DB, Brown G (2003) Genetic differentiation of Portuguese tea plant using RAPD markers. Hort Sci 38:1191–1197Google Scholar
  90. Jorgensen RA, Cluster PD (1989) Modes and tempos in the evolution of nuclear ribosomal DNA: new character for evolutionary studies and new markers for genetic and population studies. Ann Mo Bot Gard 75:1238–1247Google Scholar
  91. Kabir SE, Ghosh-Hajra N, Chaudhuri TC (1991) Performance of certain clones under the agroclimatic conditions of Darjeeling. Tea Board India Tech Bull 5:1–8Google Scholar
  92. Kafkas S, Ercisxli S, Doğan Y, Ertürk Y, Haznedar A, Sekban R (2009) Polymorphism and genetic relationships among tea genotypes from Turkey revealed by amplified fragment length polymorphism markers. J Amer Soc Hort Sci 134:428–434Google Scholar
  93. Kamunya SM, Wachira FN, Pathak RS, Korir R, Sharma V, Kumar R, Bhardwaj P, Chalo R, Ahuja PS, Sharma RK (2010) Genome mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze). Tree Genet Genom 6:915–929Google Scholar
  94. Karthigeyan S, Rajkumar S, Sharma RK, Gulati A, Sud RK, Ahuja PS (2008) High level of genetic diversity among the selected accessions of tea (Camellia sinensis) from abandoned tea gardens in Western Himalaya. Biochem Genet 46:810–819PubMedGoogle Scholar
  95. Kato F, Taniguchi F, Monobe M, Ema K, Hirono H, Maeda-Yamamoto M (2008) Identification of Japanese tea (Camellia sinensis) cultivars using SSR marker. J Jap Soc Food Sci Tech 55:49–55Google Scholar
  96. Kato M (2001) Analysis of differentiation of tea using DNA markers in evergreens forest. Int Conf on O-Cha (tea) Cult Science, Shizuoka, Japan, p 18Google Scholar
  97. Katoh Y, Katoh M, Takeda Y, Omori M (2003) Genetic diversity within cultivated teas based on nucleotide sequence comparison of ribosomal RNA maturase in chloroplast DNA. Euphytica 134:287–295Google Scholar
  98. Kaundun SS, Matsumoto S (2002) Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis. Genome 45:1041–1048PubMedGoogle Scholar
  99. Kaundun SS, Matsumoto S (2003a) Development of CAPS markers based on three key genes of the phenylpropanoid pathway in tea, Camellia sinensis (L.) O. Kuntze, and differentiation between assamica and sinensis varieties. Theor Appl Genet 106:375–383Google Scholar
  100. Kaundun SS, Matsumoto S (2003b) Identification of processed Japanese green tea based on polymorphism generated by STS-RFLP analysis. J Food Chem 51:1765–1770Google Scholar
  101. Kaundun SS, Matsumoto S (2011) Molecular evidence for maternal inheritance of the chloroplast genome in tea, Camellia sinensis (L.) O. Kuntze. J Sci Food Agric 91:2660–2663PubMedGoogle Scholar
  102. Kaundun SS, Park YG (2002) Genetic structure of six Korean tea populations revealed by RAPD-PCR markers. Crop Sci 42:594–601Google Scholar
  103. Kaundun SS, Zhyvoloup A, Park YG (2000) Evaluation of genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers. Euphytica 115:7–16Google Scholar
  104. Kerio LC, Wachira FN, Wanyoko JK, Rotich MK (2012). Characterization of anthocyanins in Kenyan teas: Extraction and identification. Food Chem 131:31–38Google Scholar
  105. Kondo K (1975) Cytological studies in cultivated species of Camellia. PhD thesis, University of North Carolina Chapel Hill, p 260Google Scholar
  106. Kondo K (1977) Chromosome number in the genus Camellia. Biotrapica 9:86–94Google Scholar
  107. Kondo K (1978a) Cytological studies in cultivated species of Camellia. In: Encyclopedia of Camellia Japan. Camellia Soc, vol 2. Kodansha Publ Co., Tokyo, p 456Google Scholar
  108. Kondo K (1978b) Cytological studies in cultivated species of Camellia. Shi-Kaki 99:41–53Google Scholar
  109. Kondo K, Parks CR (1979) Giemsa C-banding and karyotype of Camellia (-banded karyotypes can tell more detail on inter and intra-specific relationships in Camellia). Am Camellia Yearb 34:42–47Google Scholar
  110. Kondo K, Parks CR (1980) Giemsa C-banding and karyotype of Camellia. Proc Inter Camellia Cong Kyoto, pp 55–57Google Scholar
  111. Kondo K, Parks CR (1981) Cytological studies in cultivated species of Camellia. VI. Giemsa C-banded karyotypes of seven accessions of Camellia japonica L. sensu lato. Jap J Breed 31:25–34Google Scholar
  112. Kulasegaram S (1980) Technical developments in tea production. Tea Q 49:157–183Google Scholar
  113. Lai JA, Yang WC, Hsiao JY (2001) An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Bot Bull Acad Sin 42:93–100Google Scholar
  114. Latip, SNH, Muhamad R, Manjeri G, Tan SG (2010) Genetic variation of selected Camellia sinensis (cultivated tea) varieties in Malaysia based on random amplifed microsatellite (RAMs) markers. Pertanika J Trop Agric Sci 33:259–267Google Scholar
  115. Lee S, Kim J, Sano J, Ozaki Y, Okubo H (2003) Phylogenic relationships among tea cultivars based on AFLP analysis. J Food Agri 47:289–299Google Scholar
  116. Li B, Yin Y, Zhou Y, Deng PQ, Yang HW (2003) Genetic diversity of two sexual tea cultivars detected by RAPD markers. J Tea Sci 23:46–150Google Scholar
  117. Li J, Jiang CJ, Wang ZX (2005) RAPD analysis on genetic diversity of the preconcentrated core germplasms of Camellia sinensis in China. Yi Chuan 27:765–771PubMedGoogle Scholar
  118. Li JB, Hashimoto F, Shimizu K, Sakata Y (2013) Chemical taxonomy of red-flowered wild Camellia species based on floral anthocyanins. Phytochemistry 85:99–106PubMedGoogle Scholar
  119. Li JH, Nesumi A, Shimizu K, Sakata Y, Liang MZ, He QY, Zhou HJ, Hashimoto F (2010) Chemosystematics of tea trees based on tea leaf polyphenols as phenetic markers. Phytochemistry 71:1342–1349PubMedGoogle Scholar
  120. Li L, Zhong YH, Ji YZ, Zhi YZ, Sui N (2012) Analysis on genetic diversity of ten insular populations of Camellia japonica. Acta Hort Sinica 39:1531–1539Google Scholar
  121. Li XH, Zhang CZ, Liu CL, Shi ZP, Luo JW, Chen X (2007) RAPD analysis of the genetic diversity in Chinese tea germplasm. Acta Hort Sinica 34:507–508Google Scholar
  122. Liang YR, Tanaka JC, Takeda YY (2000) Study on diversity of tea germplasm by RAPD method. J Zhejiang For Coll 17:215–218Google Scholar
  123. Lin J, Kudrna D, Wing RA. (2011) Construction, characterization, and preliminary BAC-end sequence analysis of a bacterial artificial chromosome library of the tea plant (Camellia sinensis). J Biomed Biotech 2011:476723–476731Google Scholar
  124. Lin XY, Peng QF, Tang X, Hu ZH (2008) Leaf anatomy of Camellia sect. Oleifera and sect. Paracamellia (Theaceae) with reference to their taxonomic significance. J Syst Evol 46:183–190Google Scholar
  125. Lin YP, Hu CY, Tsai YZ, Lin SF (2010) Studies on the fast molecular identification technologies of made tea (Camellia sinensis) varieties and their applications. Crop Envir Bioinform 7:37–51Google Scholar
  126. Liu B, Sun X, Wang Y, Li Y, Cheng H, Xiong C, Wang P (2012b) Genetic diversity and molecular discrimination of wild tea plants from Yunnan Province based on inter-simple sequence repeats (ISSR) markers. Afr J Biotech 11:11566–11574Google Scholar
  127. Liu BY, Li YY, Tang YC, Wang LY, Cheng H, Wang PS (2010) Assessment of genetic diversity and relationship of tea germplasm in Yunnan as revealed by ISSR markers. Acta Agro Sinica 36:391–400Google Scholar
  128. Liu BY, Wang PS, Ji PZ, Xu M, Cheng H (2008) Study on genetic diversity of peculiar sect. Thea (L.) Dye in Yunnan by ISSR markers. J Yunn Agri Uni 23:302–308Google Scholar
  129. Liu Y, Yang SX, Ji PZ, Gao LZ (2012a) Phylogeography of Camellia taliensis (Theaceae) inferred from chloroplast and nuclear DNA: insights into evolutionary history and conservation. BMC Evol Biol 12:92–117Google Scholar
  130. Lou ZC, Shin YL, Ying LC (2004) Genetic relationship of Taiwan tea varieties. In: Proceedings of the 2004 international conference on O-CHA culture and science, 4–6 November, Shizuoka, Japan, pp. 226–228Google Scholar
  131. Lu H, Jiang W, Ghiassi M, Lee S, Nitin M (2012) Classification of Camellia (Theaceae) species using leaf architecture variations and pattern recognition techniques. PLoS One 7:29704–29722Google Scholar
  132. Luo JW, Shi ZP, Li JX, Shen CW, Huang YH, Gong ZH (2002a) Study on the application of RAPD techniques to parentage identification of tea plant. J Hunan Agric Univ 3:3–6Google Scholar
  133. Luo JW, Shi ZP, Shen CW, Liu CL, Gong ZH, Huang YH (2002b) Studies on genetic relationships of tea cultivars (Camellia sinensis (L.) O. Kubtze) by RAPD analysis. J Tea Sci 22:140–146Google Scholar
  134. Luo JW, Shi ZP, Shen CW, Liu CL, Gong ZH, Huang YH, Luo JW, Shi ZP, Shen CW, Liu CL, Gong ZH, Huang YH (2004) The genetic diversity of tea germplasms [Camellia sinensis (L.) O. Kuntze] by RAPD analysis. Acta Agro Sin 30:266–269Google Scholar
  135. Luo XY, Zhuang XY, Yang YS (2007) Genetic diversity of Camellia Changii Ye (Theaceae) using ISSR marker. J Tea Sci 15(2):1005–1012Google Scholar
  136. Ma JQ, Zhou YH, Ma CL, Yao MZ, Jin JQ, Wang XC, Chen L (2010) Identification and characterization of 74 novel polymorphic EST-SSR markers in the tea plant, Camellia sinensis (Theaceae). Ame J Bot 97:153–156Google Scholar
  137. Ma, JQ, Ma CL, Yao MZ, Jin JQ, Wang ZL, Wang XC, Chen L (2012) Microsatellite markers from tea plant expressed sequence tags (ESTs) and their applicability for cross-species/genera amplification and genetic mapping. Sci Hort 134:167–175Google Scholar
  138. Magambo MJS, Cannell MGR (1981) Dry matter production and partition in relation to yield of tea. Exper Agri 17: 33–38Google Scholar
  139. Magoma GN, Wachira FN, Imbuga MO, Agong SG (2003) Biochemical differentiation in Camellia sinensis and its wild relatives as revealed by isozyme and catechin patterns. Biochem System Ecol 31:995–1010Google Scholar
  140. Magoma GN, Wachira FN, Obanda M, Imbuga M, Agong SG (2000) The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genetic Resour Crop Evol 47:107–114Google Scholar
  141. Mariya JKM, Sasikumar R, Balasubramanian M, Saravanan M, Rajkumar R (2003) Influence of light on catechin biosynthesis in tea. Tea 24:80–86Google Scholar
  142. Matsumoto S, Kiriwa Y, Yamaguchi S (2004) The Korean tea plant (Camellia sinensis): RFLP analysis of genetic diversity and relationship to Japanese tea. Breed Sci 54:231–237Google Scholar
  143. Matsumoto S, Kiriwa Y, Takeda Y (2000) Analysis of genetic diversity in Chinese tea (Camellia sinensis) using RFLP and detection of difference on tea from Japanese. Breed Sci 2:209Google Scholar
  144. Matsumoto S, Kiriwa Y, Takeda Y (2002) Differentiation of Japanese green tea as revealed by RFLP analysis of phenyl-alanine ammonia-lyase DNA. Theo Appl Genet 104:998–1002Google Scholar
  145. Matsumoto S, Takeuchi A, Hayastsu M, Kondo S (1994) Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probes. Theor Appl Genet 89:671–675PubMedGoogle Scholar
  146. Matteo C, Marinoni T, Daniela VS (2010) Microsatellite-based genetic relationships in the genus Camellia: potential for improving cultivars. Genome 53:384–399Google Scholar
  147. McKenzie JS, Jurado JM, Pablos FD (2010) Characterization of tea leaves according to their total mineral content by means of probabilistic neural networks. Food Chem 123(3):859–864Google Scholar
  148. Meksen K, Leister D, Peleman J, Zabeau M, Salamini F, Gebhardt C (1995) A high resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers. Mol Gen Genet 249:74–81Google Scholar
  149. Mewan KM, Liyanage AC, Everard JMDT, Gunasekara MTK, Karunanayake EH (2005) Studying genetic relationships among the tea (Camellia sinensis) cultivars in Sri Lanka using RAPD Markers. Sri Lanka J Tea Sci 70(1):42–53 Google Scholar
  150. Mewan KM, Saha MC, Konstatin C, Pang Y, Abeysinghe ISB, Dixon RA (2007) Construction of genomic and EST-SSR based genetic linkage map of tea (Camellia sinensis). In: Proceedings of the 4th international conference on o-cha (tea) culture and science, 2–4 November 2007, Shizuoka, Japan, p 52Google Scholar
  151. Ming TL (2000) Monograph of the genus Camellia. Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Science and Technology Press, KunmingGoogle Scholar
  152. Mishra RK, Chaudhary S, Ahmad A, Pradhan M, Siddiqi TO (2009) Molecular analysis of tea clones (Camellia sinensis) using AFLP markers. Int J Integr Boil 5:130–135Google Scholar
  153. Mishra RK, Sen-Mandi S (2001) DNA fingerprinting and genetic relationship study of tea plants using amplified fragment length polymorphism (AFLP) technique. Ind J Plant Genet Resour 14:148–149Google Scholar
  154. Mishra RK, Sen-Mandi S (2004) Molecular profiling and development of DNA marker associated with drought tolerance in tea clones growing in Darjeeling. Curr Sci 87:60–66Google Scholar
  155. Mohanpuria P, Yadav SK (2012) Characterization of novel small RNAs from tea (Camellia sinensis L.). Mol Biol Rep 39:3977–986PubMedGoogle Scholar
  156. Mondal TK (2000) Studies on RAPD marker for detection of genetic diversity, in vitro regeneration and Agrobacterium-mediated genetic transformation of tea (Camellia sinensis). PhD thesis, Utkal University, IndiaGoogle Scholar
  157. Mondal TK (2002) Detection of genetic diversity among the Indian tea (Camellia sinensis) germplasm by inter-simple sequence repeats (ISSR). Euphytica 128:307–315Google Scholar
  158. Mondal TK, Bhattacharya A, Laxmikumaran M, Ahuja PS (2004) Recent advance in tea Biotechnology. Plant Cell Tiss Org 75:795–856Google Scholar
  159. Mondal TK, Chand PK (2002) Detection of genetic instability among the micropropagated tea (Camellia sinensis) plants. In Vitro Cell Dev-Pl 37:1–5Google Scholar
  160. Mondal TK, Singh HP, Ahuja PS (2000) Isolation of genomic DNA from tea and other phenolic rich plants. J Plantation Crops 28:30–34Google Scholar
  161. Mphangwe NIK, Vorster J, Steyn JM, Nyirenda HE, Taylor NJ, Apostolides Z (2013) Screening of tea (Camellia sinensis) for trait-associated molecular markers. Appl Biochem Biotechnol 171(2):437–449. doi: 10.1007/s12010-013-0370-4PubMedGoogle Scholar
  162. Mugnai S, Pandolfi C, Azzarello E, Masi E, Mancuso S (2008) Camellia japonica L. genotypes identified by an artificial neural network based on phyllometric and fractal parameters. Plant Syst Evol 270:95–108Google Scholar
  163. Mukhopadhyay M., Sarkar B, Mondal T.K (2014) Omics advances in tea (Camellia sinensis). In: Omics applications in Crop Sciences (Ed. Barh D) CRC press. New York. pp 467-490Google Scholar
  164. Muoki RC, Wachira FN, Pathak RS, Kamunya SM (2007) Assessment of the mating system of Camellia sinensis in biclonal seed orchards based on PCR markers. J Hort Sci Biotech 82:733–738Google Scholar
  165. Nagarajah S, Ratnasurya R (1981) Clonal variability in root growth and drought resistance in tea (Camellia sinensis). Plant Soil 60:153–155Google Scholar
  166. Nelson N, Melson H, Schatz G (1980) Biosynthesis and assembly of the protein-translocating adenosine triphosphete complex from chloroplasts. Proc Natl Acad Sci 77:1361–1364PubMedGoogle Scholar
  167. Neog B, Yadav RNS, Singh ID (2004) Peroxidase, polyphenol oxidase and acid phosphatase activities in the stigma-style tissue of Camellia sinensis (L) O. Kuntze following compatible and incompatible pollination. J Indian Inst Sci 84:47–52Google Scholar
  168. Ng Etich WK, Wachira FN (2003) Variations in leaf anatomy and gas exchange in tea clones with different ploidy. J Hort Sci Biotech 78:173–176Google Scholar
  169. Ohsako T, Ohgushi T, Motosugi H, Oka K (2008) Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O. Kuntze, in Kyoto, Japan. Genet Resour Crop Evol 55:1047–1053Google Scholar
  170. Orel G, Marchant AD, Wei CF, Curry AS (2007) Molecular Investigation and Assessment of C. azalea (syn. C. changii Ye 1985) as potential breeding material. Int Camellia J 39:64–75Google Scholar
  171. Otaghvari AM, Garehyazie B, Hassanpur M, Sehgal D (2010) Isozyme banding patterns in Iranian tea clones. Bioinfolet 7:7–12Google Scholar
  172. Owuor PO (1989) Differentiation of teas by the variations of linalools and geranol contents. Bull Chem Soc Ethip 3:31–35Google Scholar
  173. Owuor PO, Obanda M (1998) The use of chemical parameters as criteria for selecting for quality in clonasl black tea in Kenya: achievements, problems and prospects. A Rev Tea 19:49–58Google Scholar
  174. Owuor PO, Reeves SG, Wanyoko JK (1986) Co-relation of flavins content and valuation of Kenyan black teas. J Sci Food Agri 37: 507–513Google Scholar
  175. Pandolfi C, Mugnai S, Azzarello E, Bergamasco S, Masi E, Mancuso S (2009) Artificial neural networks as a tool for plant identification: a case study on Vietnamese tea accessions, Euphytica 166:411–421Google Scholar
  176. Park YG, Kaundun SS, Zhyvoloup A (2002) Use of the bulked genomic DNA-based RAPD methodology to assess the genetic diversity among abandoned Korean tea plantations. Genet Res Crop Evol 49:159–165Google Scholar
  177. Parks CR, Case KF (1968) Chromatographic evidence for the genetic contamination of Camellia saluenesis in cultivation. Am Camellia Yearb 23:124–134Google Scholar
  178. Paul S, Wachira FN, Powell W, Waugh R (1997) Diversity and genetic differentiation among population of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor Appl Genet 94:255–263Google Scholar
  179. Pedro L, Caceres F, Martin MJ, Pablos F, Gonzalez G (2001) Differentiation of tea (Camellia sinensis) varieties and their geographical origin according to their metal content. J Agri Food Chem 49:4775–4779Google Scholar
  180. Pi E, Peng Q, Lu H, Shen J, Du Y, Huang F, Hu H (2009) Leaf morphology and anatomy of Camellia section Camellia (Theaceae). Bot J Linn Soc 159:456–476Google Scholar
  181. Piyasundara JHN, Gunasekare MTK, Wikremasinghe IP (2008) Identification of discriminating morphological descriptors for characterization of tea (Camellia sinensis L.) germplasm in Sri Lanka. Trop Agric Res 20:193–199Google Scholar
  182. Piyasundara, JHN, Gunasekare MTK, Peiris TUS, Wickramasinghe IP (2006) Phenotypic diversity of Sri Lankan tea (Camellia sinensis L) germplasm based on morphological descriptors. Trop Agri Res 18:237–243Google Scholar
  183. Prabu GR, Mandal AK (2010) Computational identification of miRNAs and their target genes from expressed sequence tags of tea (Camellia sinensis). Genom Proteom Bioinform 8:113–121Google Scholar
  184. Prince LM, Parks CR (1997) Evolutionary relationships in the tea subfamily Theoideae based on DNA sequence data. Int Camellia J 29:135–144Google Scholar
  185. Prince LM, Parks CR (2000) Estimation on Relationships of Theoideae (Theaceae) infreed from DNA data. Int Camellia J 32:79–84Google Scholar
  186. Prince ML, Parks CR (2001) Phylogenic relationships of Theaceae inferred from chloroplast DNA sequence data. Amer J Bot 88:2309–2320Google Scholar
  187. Raina SN, Ahuja PS, Sharma RK, Das SC, Bhardwaj P, Negi R, Sharma V, Singh SS, Sud RK, Kalia RK, Pandey V, Banik J, Razdan V, Sehgal D, Dar TH, Kumar A, Bali S, Bhat V, Sharma S, Prasanna BM, Goel S, Negi MS, Vijayan P, Tripathi SB, Bera B, Hazarika M, Mandal AKA, Kumar RR, Vijayan D, Ramkumar S, Chowdhury BR, Mandi SS (2012) Genetic structure and diversity of India hybrid tea. Genet Resour Crop Evol 59:1527–1541Google Scholar
  188. Rajanna L, Ramakrishnan M (2010) Isozyme studies on some selected Camellia clones. Int J Eng Sci Tech 2:6918–6921Google Scholar
  189. Rajanna L, Ramakrishnan M, Simon L (2011) Evaluation of morphological diversity in south Indian tea clones using statistical methods. Maejo Int J Sci Technol 5(1):1–12Google Scholar
  190. Rajasekaran P (1997) Development of molecular markers using AFLP in tea. In: Varghese JP (ed) Molecular approaches to crop improvement. Proceedings of the national seminar on molecular approaches to crop improvement 29–31 Dec, Kottayam, Kerala, India, pp 54–58Google Scholar
  191. Rani V, Ajay P, Raina SN (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Rep 14:459–462PubMedGoogle Scholar
  192. Roberts EAH, Wight W, Wood DJ (1958) Paper chromatography as an aid to the identification of Thea camellias. New Phytol 57:211–225Google Scholar
  193. Rokas A, King N, Finnerty J, Carroll SB (2003a) Conflicting phylogenetic signals at the base of the metazoan tree. Evol Dev 5:346–359Google Scholar
  194. Rokas A, Williams BL, King N, Carroll SB (2003b) Genome scale approaches to resolving incongruence in molecular phylogenies. Nature 425:798–804Google Scholar
  195. Roy SC, Chakraborty BN (2007) Evaluation of genetic diversity in tea of the Darjeeling foot hills, India using RAPD and ISSR markers. J Hill Res 20:13–19Google Scholar
  196. Roy SC, Chakraborty BN (2009) Genetic diversity and relationship among tea (Camellia sinensis) cultivars as revealed by RAPD and ISSR based fingerprinting. Indian J Biotech 8:370–376Google Scholar
  197. Sabhapondit S, Karak T, Bhuyan LP, Goswami BC, Hazarika M (2012) Diversity of catechin in Northeast Indian tea cultivars. Sci World J. doi:10.1100/2012/485193Google Scholar
  198. Sanderson GW (1964) The chemical composition of fresh tea flush as affected by clone and climate. Tea Quart 35:101–109Google Scholar
  199. Saravanan M, Maria John KM, Raj Kumar R, Pius PK, Sasikumar R (2005) Genetic diversity of UPASI tea clones (Camellia sinensis (L.) O. Kuntze) on the basis of total catechins and their fractions. Phytochem 66:561–565Google Scholar
  200. Satyanarayan N, Sharma VS (1982) Biometric basis for yield prediction in tea clonal selection. Proceedings of the 14th Plantation Crops Symposium PLACROSYM IV, 3–5 Dec 1981, Mysore, India, pp 237–243Google Scholar
  201. Satyanarayan N, Sharma VS (1986) Tea (Camellia L. spp) germplasm in South India. In: Srivastava HC, Vatsya B, Menon KKG (eds) Plantation crops: opportunity and constraints. Oxford IBH Publishing Co, New Delhi, pp 173–179Google Scholar
  202. Scowcroft WR (1984) Genetic variability in tissue culture: impact on germplasm conservation and utilization. Report on the IBPGR, Rome, p 152Google Scholar
  203. Sealy JR (1958) A revision of the genus Camellia. Royal Horticultural Society, LondonGoogle Scholar
  204. Sen P, Bora U, Roy BK, Deka PC (2000) Isozyme characterization in Camellia spp. Crop Res 19:519–524Google Scholar
  205. Seurei P (1996) Tea improvement in Kenya: a review. Tea 17:76–81Google Scholar
  206. Shao WF, Pang RH, Duan HX, Wang PS, Xu M, Zhang YP, Li JH (2003a) RAPD analysis of tea in Yunnan. Sci Agric Sinica 36:1582–1587Google Scholar
  207. Shao WF, Pang RH, Duan HX, Wang PS, Xu M, Zhang YP, Li JH (2003b) Use of RAPD analysis to classify tea trees in Yunnan. Agric Sci China 2:1290–1296Google Scholar
  208. Sharma P, Deka PC (2002) Identification of tea hybrids by leaf and pollen isozyme analysis. Res Crop 3:411–415Google Scholar
  209. Sharma RK, Bhardwaj P, Negi R, Mohapatra T, Ahuja PS (2009) Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis L.). BMC Plant Biol 9:53–77PubMedCentralPubMedGoogle Scholar
  210. Sharma RK, Negi MS, Sharma S, Bhardwaj P, Kumar R, Bhattachrya E, Tripathi SB, Vijayan D, Baruah AR, Das SC, Bera B, Rajkumar R, Thomas J, Sud RK, Muraleedharan N, Hazarika M, Lakshmikumaran M, Raina SN, Ahuja PS (2010) AFLP-based genetic diversity assessment of commercially important tea germplasm in India. Biochem Genet 48:549–564PubMedGoogle Scholar
  211. Sharma RKK, Negi MS, Sharma S, Bhardwaj P, Kumar R, Bhattachrya E, Tripathi SB, Vijayan D, Baruah AR, Das SC, Bera B, Rajkumar R, Thomas J, Sud RK, Muraleedharan N, Hazarika M, Sharma H, Kumar R, Sharma V, Kumar V, Bhardwaj P, Ahuja PS, Sharma RK (2011) Identification and cross-species transferability of 112 novel unigene-derived microsatellite markers in tea (Camellia sinensis). Amer J Bot 98:133–138Google Scholar
  212. Shen CW, Huang YH, Huang JA, Luo JW, Liu CL, Liu DH (2007) RAPD analysis for genetic diversity of typical tea populations in Hunan Province. J Agri Biotech 15:855–860Google Scholar
  213. Shen CW, Luo JW, Shi ZP, Gong ZH, Tang HP, Liu FZ, Huang YH (2002) Study on genetic polymorphism of tea plants in Anhua Yuntaihan population by RAPD. J Hunan Agric Univ 28:320–325Google Scholar
  214. Shen CW, Ning ZX, Huang JA, Chen D, Li JX. (2009) Genetic diversity of Camellia sinensis germplasm in Guangdong Province based on morphological parameters and SRAP markers. Ying Yong Sheng Tai Xue Bao 20:1551–1558PubMedGoogle Scholar
  215. Shi J, Dai X, Chen Y, Chen J, Shi J, Yin T (2013) Discovery and experimental analysis of microsatellites in an oil woody plant Camellia chekiangoleosa. Plant Syst Evol 299:1387–1393Google Scholar
  216. Shi SH, Tang SQ, Chen YQ, Qu LH, Chang HT (1998) Phylogenic relationship among yellow flowered Camellia species based on random polymorphic DNA. Acta Phytotaxo Sinica 36:317–322Google Scholar
  217. Shu J, Chen L (1996) Study on the evolution route of tea pollen morphology. J Tea Sci 16:115–118Google Scholar
  218. Singh D, Ahuja PS (2006) 5S rDNA gene diversity in tea (Camellia sinensis (L.) O. Kuntze) and its use for variety identification. Genome 49:91–96PubMedGoogle Scholar
  219. Singh HP, Ravindranath SD (1994) Occurrence and distribution of PPO activity in floral organs of some standard and local cultivars of tea. J Sci Food Agric 64:117–120Google Scholar
  220. Singh ID (1980) Nonconventional approaches to the breeding of tea in north-east India. Two Bud 27:3–6Google Scholar
  221. Singh ID (1999) Plant improvement. In: Jain NK (ed) Global advances in tea. Aravali Book International (P) Ltd., Delhi, pp 427–448Google Scholar
  222. Singh M, Bandana, Ahuja PS (1999) Isolation and PCR amplification of genomic DNA from market samples of dry tea. Plant Mol Biol Rep 17:171–178Google Scholar
  223. Singh M, Dhiman B, Sharma C (2011) Characterization of a highly repetitive DNA sequence in Camellia sinensis (L.) O. Kuntze genome. J Biotech Res 3:78–83Google Scholar
  224. Singh M, Saroop J, Dhiman B (2004) Detection of intra-clonal genetic variability in vegetatively propagated tea using RAPD markers. Biologia Plant 48:113–115Google Scholar
  225. Singh S, Sud RK, Gulati A, Joshi R, Yadav AK, Sharma RK (2013) Germplasm appraisal of western Himalayan tea: a breeding strategy for yield and quality improvement. Genet Resour Crop Evol 60:1501–1513Google Scholar
  226. Smith MK (1998) A review of factors influencing the genetic stability of micropropagated banana fruits. Aust J Expt Agric 43:219–223Google Scholar
  227. Staub JE, Kuhns LJ, May B, Grun P (1982) Stability of potato tuber isozymes under different storage regimes. J Am Soc Hort Sci 107:405–408Google Scholar
  228. Su MH, Hsieh CF, Tsou CH (2009) The confirmation of Camellia formosensis (Theaceae) as an independent species based on DNA sequence analyses. Bot Stud 50:477–485Google Scholar
  229. Su MH, Tsou CH, Hsieh CF (2007) Morphological comparisons of Taiwan native wild tea plant (Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura) and two closely related taxa using numerical methods. Taiwania 52:70–83Google Scholar
  230. Taberlet P, Gielly L, Pauton G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of choloroplast DNA. Plant Mol Biol 17:1105–1109PubMedGoogle Scholar
  231. Takeo T (1981) Variations in amounts of linalool and geraniol produced in tea shoots by mechanical injury. Phytochemistry 30:2149–2151Google Scholar
  232. Takeo T (1983) Variation in the aroma compound content of semi-fermented tea and black tea. Nippon Nogei Kagaku Kaishi 57:457–459Google Scholar
  233. Tanaka J (1996) RAPD linkage map of tea plant and the possibility of application in tea genetics and breeding. Tea Res J 84:44–45Google Scholar
  234. Tanaka J (2006) Study on the utilization of DNA markers in tea breeding. Bull Nat Inst Veg Tea Sci 5:113–155Google Scholar
  235. Tanaka J, Taniguchi F (2002) Emphasized-RAPD (e-RAPD): a simple and efficient technique to make RAPD Bands clearer. Breed Sci 52:225–229Google Scholar
  236. Tanaka J, Yamaguchi N, Nakamura Y (2001) Pollen parent of tea cultivar Sayamakaori with insect and cold resistance may not exist. Breed Res 3:43–48Google Scholar
  237. Tanaka JI, Sawai Y, Yamaguchi S (1995) Genetic analysis of RAPD markers in tea. J Jap Breed 45:198–199Google Scholar
  238. Tanaka JI, Yamaguchi S (1996) Use of RAPD markers for the identification of parentage of tea cultivars. Bull Nat Res Inst Veg Orna Plant Tea 9:31–36Google Scholar
  239. Tang S, Bin X, Wang L, Zhong Y (2006) Genetic diversity and population structure of yellow Camellia (Camellia nitidissima) in China as revealed by RAPD and AFLP markers. Biochem Genet 44:449–461PubMedGoogle Scholar
  240. Taniguchi F, Furukawa K, Ota-Metoku S, Yamaguchi N, Ujihara T, Kono I, Fukuoka H, Tanaka J (2012) Construction of a high-density reference linkage map of tea (Camellia sinensis). Breed Sci 62: 263–273PubMedCentralPubMedGoogle Scholar
  241. Taniguchi F, Tanaka J, Kono I (2007) Construction of genetic linkage map of tea using SSR markers. In: Proceedings of the 3rd international conference on o-cha (tea) culture and science (ICOS), Shizuoka, JapanGoogle Scholar
  242. Tanikawa N, Onozaki T, Nakayama M, Shibata M (2008) PCR-RFLP analysis of chloroplast DNA variations in the atpI-atpH spacer region of the Genus Camellia. J Jap Soc Hort Sci 77:408–417Google Scholar
  243. Tanksley SD, Yong ND, Paterson AH, Bonierbals MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotech 7: 257–264Google Scholar
  244. Tateishi N, Ozaki Y, Okubo H (2007) Occurrence of ploidy variation in Camellia vernalis. J Fac Agric Kyushu Univ 52:11–15Google Scholar
  245. Thakor BH (1997) A re-examination of the phylogenetic relationships within the genus Camellia. Int Camellia J 29:130–134Google Scholar
  246. Thomas J, Vijayan D, Joshi SD, Joseph Lopez S, Raj Kumar R (2006) Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats. J Biotech 123:149–154Google Scholar
  247. Tian M, Ll JY, NI S Li XL (2008) Phylogenetic study on section Camellia based on ITS sequences data. J Acta Hort Sinica 35:1685–1688Google Scholar
  248. Tiao JX, Parks CR (1997) Identification of closely related Camellia hybrid and mutant using molecular markers. Int Camellia J 29:111–116Google Scholar
  249. Tiao JX, Parks CR (2003) Research for a new classification system for the genus Camellia. Int Camellia J 33:109–112Google Scholar
  250. Toyao T, Takeda Y (1999) Studies on geographical diversity of floral morphology of tea plant (Camellia sinensis (L.) O. Kuntze) using the method of numerical taxonomy. Tea Res J 87:39–57Google Scholar
  251. Tsumura Y, Ohba K, Strauss SH (1996) Diversity and inheritance of Inter-simple sequence repeat polymorphism in Douglas fir (Pseudotsuga menziessi) and sugi (Cryptomeria japonica). Theor Appl Genet 92:40–45PubMedGoogle Scholar
  252. Ueno S, Tomaru N, Yoshimaru H, Manabe T, Yamamoto S (2000) Genetic structure of Camellia japonica L. in an old-growth evergreen forest, Tsushima, Japan. Mol Eco 9:647–656Google Scholar
  253. Ueno S, Tsumura Y (2009) Development of microsatellite and amplicon length polymorphism markers for Camellia japonica L. from tea plant (Camellia sinensis) expressed sequence tags. Mol Ecol Resour 9:814–816PubMedGoogle Scholar
  254. Ueno S, Yoshimaru H, Tomaru N, Yamamoto S (1999) Development and characterization of microsatellite markers in Camellia japonica L. Mole Eco 8:335–336Google Scholar
  255. Ujihara T, Taniguchi F, Tanaka J, Hayashi N (2011). Development of expressed sequence tag (EST)-based Cleaved Amplified Polymorphic Sequence (CAPS) markers of tea plant and their application to cultivar identification. J Agric Food Chem 59:1557–1564PubMedGoogle Scholar
  256. Vijayan K, Tsou CH (2008) Technical report on the molecular phylogeny of Camellia with nrITS: the need for high quality DNA and PCR amplification with Pfu-DNA polymerase. Bot Stud 49:177–188Google Scholar
  257. Vijayan K, Zhang WJ, Tsou CH (2009) Molecular taxonomy of Camellia (Theaceae) inferred from NRITS sequences. Amer J Bot 96:1348–1360Google Scholar
  258. Vo TD (2006) Assessing genetic diversity in Vietnam tea [Camellia sinensis (L.) O. Kuntze] using morphology, inter-simple sequence repeat (ISSR) and microsatellite (SSR) markers. PhD Thesis, Georg-August Gottingen University, GermanyGoogle Scholar
  259. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Freijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new techniques for DNA fingerprinting. Nucleic Acid Res 23:4407–4414PubMedGoogle Scholar
  260. Vuylsteke D, Swennen R, Wilson GF, Langhe ED (1988) Phenotypic variation among in vitro propagated plantain (Muse sp. Cultivar ‘AAB’). Sci Hort 36:79–80Google Scholar
  261. Wachira F, Tanaka J, Takeda Y (2001) Genetic variation and differentiation in tea (Camellia sisnensis) germplasm revealed by RAPD and AFLP variation. J Hort Sci Biotech 76:557–563Google Scholar
  262. Wachira FN (1990) Desirable tea plants: an overview of a search for markers. Tea 11:42–48Google Scholar
  263. Wachira FN, Powell W, Waugh R (1997) An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle specific STS. Heredity 78:603–611Google Scholar
  264. Wachira FN, Waugh R, Hackett CA, Powell W (1995) Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38:201–210PubMedGoogle Scholar
  265. Wang BY, Ruan ZY (2012) Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy. Genet Mol Res 11:503–511PubMedGoogle Scholar
  266. Wang XF, Zheng HY, Zheng WH, Ao CQ, Jin HY, Zhao LH, Li N, Jia LR (2011) RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China. Genet Mol Res 10:849–859PubMedGoogle Scholar
  267. Wang XF, Zheng WH, Zheng HX, Xie QQ, Zheng HY, Tang H, Tao YL (2010) Optimization of RAPD-PCR reaction system for genetic relationships analysis of 15 Camellia cultivars. Afr J Biotech 9:798–804Google Scholar
  268. Wang XP, Ma Bt, Qi GN, Tian H, Fang CU, Zhang ZC, Yin XM (2007) RAPD analysis on the genetic relationsh ips of tea cultivars grown in Sichuan. J Acta Hort Sinica 34:242–244Google Scholar
  269. Waugh R, Vande Ven WTG, Phillips MS, Powell W (1990) Chloroplasts DNA diversity in the genus Rubus (Rosaceae) revealed by southern hybridization. Plant Syst Evol 172:65–75Google Scholar
  270. Wei JQ, Chen ZY, Wang ZF, Tang H, Jiang YS, Wei X, Li XY, Qi XX (2010) Isolation and characterization of polymorphic microsatellite loci in Camellia nitidissima chi (Theaceae). Amer J Bot 97:89–90Google Scholar
  271. Wei K, Wang L, Zhou J, He W, Zeng J, Jiang Y, Cheng H (2011) Catechin contents in tea (Camellia sinensis) as affected by cultivar and environment and their relation to chlorophyll contents. Food Chem 125:44–48Google Scholar
  272. Wei X, Cao H-L, Jlang YS, Ye WH, Ge XJ, Ll F (2008) Population genetic structure of Camellia nitidissima (Theaceae) and conservation implications. Bot Stud 49:147–153Google Scholar
  273. Wei X, Wei JQ, Cao HL, Li F, Ye WH (2005) Genetic diversity and differentiation of Camellia euphlebia (Theaceae) in Guangxi, China. Ann Bot Fennici 42:365–370Google Scholar
  274. Wen Q, Xu L, Gu Y, Huang M, Xu L (2012) Development of polymorphic microsatellite markers in Camellia chekiangoleosa (Theaceae) using 454-ESTs. Am J Bot 99:203–205Google Scholar
  275. Wen SC, Wu LJ, Peng SJ, Hua GZ, Ping TH, Zhi LF, Huan HY, Shen CW, Luo JW, Shi ZP, Gong ZH, Tang HP, Liu FZ, Huang YH (2002) Study on genetic polymorphism of tea plants in Anhua Yuntaishan population by RAPD. J Hunan Agri Univ 28:320–325Google Scholar
  276. Wendel JF, Parks CR (1982) Genetic control of isozyme variation in Camellia japonica L. J Hered 73:197–204Google Scholar
  277. Wendel JF, Parks CR (1983) Cultivar identification in Camellia japonica L. using allozyme polymorphisms. J Amer Soc Hort Sci 108:290–295Google Scholar
  278. Wendel JF, Parks CR (1984) Distorted segregation and linkage of alcohol dehydrogenase genes in Camellia japonica L. (Theaceae). Biochem Genet 22:739–748PubMedGoogle Scholar
  279. Wendel JF, Parks CR (1985) Genetic diversity and population structure in Camellia japonica L. (Theaceae). Amer J Bot 72:52–65Google Scholar
  280. Wickramaratna MRT (1981) Variations in some leaf characteristics in tea (Camellia sinensis L.) and their use in identification of clones. Tea Quart 50:183–198Google Scholar
  281. Wight W (1954) Morphological basis of quality in tea. Nature 173:630–631Google Scholar
  282. Wight W (1958) The agrotype concept in tea taxonomy. Nature 181:893–895Google Scholar
  283. Wight W, Barua DN (1954) Morphological basis of quality in tea. Nature 173:630–631Google Scholar
  284. Williams JGK, Kubelik AR, Livak KJ, Rafaliski JA, Tingey SV (1990) DNA polymorphism amplified by arbitory primers are useful as genetic markers. Nucl Acid Res 18:6531–6535Google Scholar
  285. Wright LP, Apostolides Z, Louw AI (1996) DNA fingerprinting of tea clones. In: Whittle AM and Khumalo FRB (eds). Proceedings of the 1st Regional Tea Research Seminar. Blantyre, Malawi 22–23 March 1995, pp. 44–50Google Scholar
  286. Wright LP, Mphangwe NIK, Nyirenda HE, Apostolides Z (2000) Analysis of caffeine and flavan-3-ol composition in the fresh leaf of Camellia sinesis for predicting the quality of the black tea produced in Central and Southern Africa. J Sci Food Agric 80:1823–1830Google Scholar
  287. Wright LP, Mphangwe NIK, Nyirenda HE, Apostolides Z (2002) Analysis of the theaflavin composition in black tea (Camellia sinensis) for predicting the quality of tea produced in Central and Southern Africa. J Sci Food Agric 82:517–525Google Scholar
  288. Wu H, Chen D, Li J, Yu B, Qiao X, Huang H, He Y (2013). De novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea (Camellia sinensis) Plant Mol Biol Rep 31:524–538Google Scholar
  289. Wu KS, Tanksley SD (1993) Abundance, polymorphism and genetic mapping of microsatellite in rice. Mol Genet 241:225–235Google Scholar
  290. Wu LJ, Peng SJ, Wen SC, Lin LC, Hua GZ, Huan HY, Luo JW Shi ZP, Shen CW, Liu CL, Gong ZH, Huang YH (2002a) Studies on genetic relationships of tea cultivars [Camellia sinensis (L.) O. Kuntze] by RAPD analysis. J Tea Sci 22:140–146Google Scholar
  291. Wu LJ, Peng SZ, Xian Li J, Wen SC, Huan HY, Hua GZ, Luo JW, Shi ZP, Li JX, Shen CW, Huang YH, Gong ZH (2002b) Studies on genetic diversity of tea cultivars [Camellia sinensis (L.) O. Kuntze] by RAPD analysis. J Tea Sci 12:121–127Google Scholar
  292. Xiao LZ, Yan CY, Li JX, Luo JW, He YM, Zhao CY (2007) AFLP analysis on genetic diversity of Fenghuang-Dancong tea plant germplasm. J Tea Sci 27:280–285Google Scholar
  293. Xiao TJ, Parks CR (2003) Molecular analysis of the genus Camellia. Int Camellia J 35:57–65Google Scholar
  294. Xu H, Ton Q, Zhuang W (1987) Studies on genetic tendency of tea plant hybrid generation using isozyme technique, Proceedings of the International Tea Quality and Health Symposium, pp 21–25Google Scholar
  295. Xu ZX, Liu ZH, Wang KB, Liu F, Shi L, Gao DZ, Xu ZX, Liu ZH, Wang KB, Liu F, Shi L, Gao DZ (2004) On relationship between green tea polyphenols and catechins and green tea raw materials. J Hunan Agri Univ 30:257–260Google Scholar
  296. Yamaguchi S (2001) Summarized remarks on the origin of Japanese tea by the genetic resource study in Eastern Asia and Japan. Proc 2001 Intl Conf O-Cha Cult Science, Shizuoka, Japan, 5–8 OctGoogle Scholar
  297. Yang JB, Li HT, Yang SX, Li DZ, Yang YY (2006) The application of four DNA sequences to studying molecular phylogeny of Camellia (Theaceae). Acta Bot Yunn 28:108–114Google Scholar
  298. Yang JB, Yang J, Li HT, Zhao Y, Yang SX (2009) Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method. Conserv Genet 10:1621–1623Google Scholar
  299. Yang Y, Sun T (1994) Study on the esterase isoenzyme in tea mutagenic breeding. China Tea 16:4–9Google Scholar
  300. Yang YJ, Yu FL, Chen L, Zeng JM, Yang SJ, Li SF, Shu AM, Zhang ZF, Wang YS, Wang HS, Wang PS, Xu M, Song WX, Guo JC, Yang RX, Zhang WJ, Chen ZH, Yang YJ, Yu FL, Chen L, Zeng JM, Yang SJ, Li SF, Shu AM, Zhang ZF, Wang YS, Wang HS, Wang PS, Xu M, Song WX, Guo JC, Yang RX, Zhang WJ, Chen ZH (2003) Elite germplasm evaluation and genetic stability of tea plants. J Tea Sci 23:1–8Google Scholar
  301. Yang Z, Goldman N, Friday A (1994) Comparison of models for nucleotide substitution used in maximum-likelihood phylogenetic estimation. Mol Biol Evol 11:316–324PubMedGoogle Scholar
  302. Yao MZ, Chen L, Liang YR (2008) Genetic diversity among tea cultivars from China, Japan and Kenya revealed by ISSR markers and its implication for parental selection in tea breeding prog. Plant Breed 127:166–172Google Scholar
  303. Yao MZ, Ma CL, Qiao TT, Jin JQ, Chen L (2012) Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers. Tree Genet Genom 8:205–220Google Scholar
  304. Yoshikawa N, Parks CR (2001) Systematic studies of Camellia japonica and closely related species. Int Camellia J 33:117–121Google Scholar
  305. Zhang GW, Zhong WB, Wu Y, Tan XF, Du TZ (2007) Identification of oil tea (Camellia oleifera) superior clones by ISSR molecular marker. Forest Res 20:278–282Google Scholar
  306. Zhang YP, Li JH (2003a) RAPD analysis of tea trees in Yunnan. Sci Agri Sin 36:1582–1587Google Scholar
  307. Zhang YP, Li JH (2003b). Use RAPD analysis to classify tea trees in Yunnan. Agri Sci China 2:1290–1296Google Scholar
  308. Zhao CY, Zhou LH, Luo JW, Huang JA, Tan HP (2006) AFLP analysis of genetic diversity of tea plant germplasm in Guangdong province. J Tea Sci 26:249–252Google Scholar
  309. Zhao LP, Liu Z, Chen L, Yao MZ, Wang XC (2008) Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties. Conserv Genet 9:1327–1331Google Scholar
  310. Zhu QW, Luo YP (2013) Identification of microRNA and their targets in tea (Camellia sinensis) J Zhejian Uni Sci-B (BioMed and Biotech) 5:1–10Google Scholar
  311. Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple-sequence repeat (SSR) anchored polymerase chain reaction amplification. Genomics 20:176–183PubMedGoogle Scholar

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© Springer India 2014

Authors and Affiliations

  1. 1.Division of Genomic ResourcesNational Bureau of Plant Genetic ResourcesDelhiIndia

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