Abstract
Plant tissue culture is the science of growing plant cells, tissues or organs excised from the mother/donor plant, on artificial media under an aseptic environment. It includes techniques and methods used to do research in many plant science disciplines and has several practical objectives. Before starting to culture plants via in vitro methods, it is necessary to have a comprehensible understanding of the ways in which plant material can be grown and manipulated in an in vitro environment. This chapter describes the techniques that have been developed for the micropropagation (clonal propagation) of the tree species Balanites aegyptiaca and shows these techniques can be applied for propagation of other tree species also. Both direct axillary shoot regeneration and adventitious regeneration are possible for clonal propagation in vitro.
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Varshney, A., Anis, M. (2014). Materials and Methods. In: Trees: Propagation and Conservation. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1701-5_3
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DOI: https://doi.org/10.1007/978-81-322-1701-5_3
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