Materials and Methods

  • Ankita Varshney
  • Mohammad Anis


Plant tissue culture is the science of growing plant cells, tissues or organs excised from the mother/donor plant, on artificial media under an aseptic environment. It includes techniques and methods used to do research in many plant science disciplines and has several practical objectives. Before starting to culture plants via in vitro methods, it is necessary to have a comprehensible understanding of the ways in which plant material can be grown and manipulated in an in vitro environment. This chapter describes the techniques that have been developed for the micropropagation (clonal propagation) of the tree species Balanites aegyptiaca and shows these techniques can be applied for propagation of other tree species also. Both direct axillary shoot regeneration and adventitious regeneration are possible for clonal propagation in vitro.


Double Distil Water Plant Growth Regulator Alginate Bead Nodal Segment ISSR Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Department of Botany Plant Biotechnology LaboratoryAligarh Muslim UniversityAligarhIndia

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