Advertisement

Materials and Methods

  • Ankita Varshney
  • Mohammad Anis
Chapter

Abstract

Plant tissue culture is the science of growing plant cells, tissues or organs excised from the mother/donor plant, on artificial media under an aseptic environment. It includes techniques and methods used to do research in many plant science disciplines and has several practical objectives. Before starting to culture plants via in vitro methods, it is necessary to have a comprehensible understanding of the ways in which plant material can be grown and manipulated in an in vitro environment. This chapter describes the techniques that have been developed for the micropropagation (clonal propagation) of the tree species Balanites aegyptiaca and shows these techniques can be applied for propagation of other tree species also. Both direct axillary shoot regeneration and adventitious regeneration are possible for clonal propagation in vitro.

Keywords

Double Distil Water Plant Growth Regulator Alginate Bead Nodal Segment ISSR Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Aebi H (1984) Catalse in vitro methods. Methods in Enzymology. Academic Press Inc 105:121–126Google Scholar
  2. Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Ann Biochem 72:248–254CrossRefGoogle Scholar
  3. Dhindsa PS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101CrossRefGoogle Scholar
  4. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  5. Foyer CH, Halliwell B (1976) The presence of glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta 133:21–25CrossRefGoogle Scholar
  6. Gamborg OL, Miller RA, Ojima K (1968) Nutrients requirement of suspension culture of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  7. Jaccard P (1908) Nouvelles rescherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270Google Scholar
  8. Johansen DA (1940) Plant microtechnique. Mc Graw-Hill Book Co. Inc., New YorkGoogle Scholar
  9. Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int Plant Prop Soc Proc 30:421–427Google Scholar
  10. Mackinney G (1941) Absorption of light by chlorophyll solution. J Biol Chem 140:315–322Google Scholar
  11. Maclachan S, Zalick S (1963) Plastid structure, chlorophyll concentration and free amino acid composition of chlorophyll mutant barley. Can J Bot 41(7):1053–1062CrossRefGoogle Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  13. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  14. Perrier X, Jacquemoud-Collet JP (2006) DARwin software, Version 5.0.158, http://darwin.cirad.fr/darwinGoogle Scholar
  15. Phillips GC, Collins GB (1979) In vitro tissue culture of selected legumes and plant regeneration from callus of red clover. Crop Sci 19:59–64CrossRefGoogle Scholar
  16. Rao MV (1992) Cellular detoxifying mechanism determines age-dependent injury in tropical plants exposed to SO2. J Plant Physiol 140:733–740CrossRefGoogle Scholar
  17. Sambrook J, Fritsch EJ, Maniatis T (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Department of Botany Plant Biotechnology LaboratoryAligarh Muslim UniversityAligarhIndia

Personalised recommendations