Whole Genome Sequencing Strategies and Development of Orbivirus Sequence Database: Implications for Novel dsRNA Virus Detection

  • Sushila Maan
  • Manjunatha N. Belaganahalli
  • Narender S. Maan
  • Peter P. C. Mertens


The genus Orbivirus is the largest of the genera within the family Reoviridae, containing 22 recognised virus species as well as 15 unclassified ‘orbiviruses’, which could potentially represent further new species. The orbiviruses are transmitted by both ticks and/or haematophagous insect vectors. They have a wide host range that includes domestic and wild ruminants, equines, marsupials, sloths, bats, birds and humans. Low-level serological cross-reactions between different species of orbiviruses and lack of reference strains/antisera for existing Orbivirus species make serological identification of new virus isolates difficult. Recently, whole genome sequence data (WGS) has become an important tool for the detection, classification and epidemiological investigations of different pathogens. This study presents full genome sequence database of all known 22 Orbivirus species (including 5 unclassified viruses). Development of novel sequencing strategies and phylogenetic analysis of the orbiviruses using this database has identified five novel Orbivirus species and has facilitated development of a pan-orbivirus RT-PCR assay that can be used to identify the RNA of any Orbivirus species. These techniques will support Orbivirus discovery with greater accuracy than before and can be used for definitive diagnosis of suspected Orbivirus infection.


Genome Segment Wide Host Range Virus Species dsRNA Virus Wild Ruminant 



This paper is based on a presentation made at the International conference on Biotechnology: Emerging Trends (ICB-2012) organized by Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, Haryana, India from 18-20th September 2012. I would like to thank the organisers for inviting me to the meeting. We are extremely grateful to the many colleagues at IAH Pirbright, who had their direct or indirect contributions to these studies.


  1. Akita GY, Chinsangaram J, Osburn BI, Ianconescu M, Kaufman R (1992) Detection of bluetongue virus serogroup by polymerase chain reaction. J Vet Diagn Invest 4:400–405PubMedGoogle Scholar
  2. Allison AB, Goekjian VH, Potgieter AC, Wilson WC, Johnson DJ, Mertens PP, Stallknecht DE (2010) Detection of a novel reassortant epizootic hemorrhagic disease virus (EHDV) in the USA containing RNA segments derived from both exotic (EHDV-6) and endemic (EHDV-2) serotypes. J Gen Virol 91:430–439PubMedGoogle Scholar
  3. Allison AB, Holmes EC, Potgieter AC, Wright IM, Sailleau C, Breard E, Ruder MG, Stallknecht DE (2012) Segmental configuration and putative origin of the reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6, strain Indiana. Virology 424:67–75PubMedGoogle Scholar
  4. Anthony SJ (2007) Genetic studies of epizootic haemorrhagic disease virus (EHDV). Linacre College, University of Oxford, OxfordGoogle Scholar
  5. Anthony S, Jones H, Darpel KE, Elliott H, Maan S, Samuel A, Mellor PS, Mertens PP (2007) A duplex RT-PCR assay for detection of genome segment 7 (VP7 gene) from 24 BTV serotypes. J Virol Methods 141:188–197PubMedGoogle Scholar
  6. Anthony SJ, Maan N, Maan S, Sutton G, Attoui H, Mertens PP (2009) Genetic and phylogenetic analysis of the core proteins VP1, VP3, VP4, VP6 and VP7 of epizootic haemorrhagic disease virus (EHDV). Virus Res 145:187–199PubMedGoogle Scholar
  7. Anthony SJ, Darpel KE, Maan S, Sutton G, Attoui H, Mertens PP (2010) The evolution of two homologues of the core protein VP6 of epizootic haemorrhagic disease virus (EHDV), which correspond to the geographical origin of the virus. Virus Genes 40:67–75PubMedGoogle Scholar
  8. Aradaib IE (2009) PCR detection of African horse sickness virus serogroup based on genome segment three sequence analysis. J Virol Methods 159:1–5PubMedGoogle Scholar
  9. Aradaib IE, Smith WL, Osburn BI, Cullor JS (2003) A multiplex PCR for simultaneous detection and differentiation of North American serotypes of bluetongue and epizootic hemorrhagic disease viruses. Comp Immunol Microbiol Infect Dis 26:77–87PubMedGoogle Scholar
  10. Aradaib IE, Mohamed ME, Abdalla TM, Sarr J, Abdalla MA, Yousof MA, Hassan YA, Karrar AR (2005) Sero-grouping of United States and some African serotypes of bluetongue virus using RT-PCR. Vet Microbiol 111:145–150PubMedGoogle Scholar
  11. Aradaib IE, Mohamed ME, Abdalla MA (2009) A single-tube RT-PCR for rapid detection and differentiation of some African isolates of palyam serogroup orbiviruses. J Virol Methods 161:70–74PubMedGoogle Scholar
  12. Arankalle VA, Shrivastava S, Cherian S, Gunjikar RS, Walimbe AM, Jadhav SM, Sudeep AB, Mishra AC (2007) Genetic divergence of Chikungunya viruses in India (1963–2006) with special reference to the 2005–2006 explosive epidemic. J Gen Virol 88:1967–1976PubMedGoogle Scholar
  13. Attoui H, Billoir F, Biagini P, de Micco P, de Lamballerie X (2000a) Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81:1507–1515PubMedGoogle Scholar
  14. Attoui H, Billoir F, Cantaloube JF, Biagini P, de Micco P, de Lamballerie X (2000b) Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89:147–158PubMedGoogle Scholar
  15. Attoui H, Stirling JM, Munderloh UG, Billoir F, Brookes SM, Burroughs JN, de Micco P, Mertens PP, de Lamballerie X (2001) Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis. J Gen Virol 82:795–804PubMedGoogle Scholar
  16. Attoui H, Mohd Jaafar F, Belhouchet M, Biagini P, Cantaloube JF, de Micco P, de Lamballerie X (2005a) Expansion of family Reoviridae to include nine-segmented dsRNA viruses: isolation and characterization of a new virus designated Aedes pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus). Virology 343:212–223PubMedGoogle Scholar
  17. Attoui H, Mohd Jaafar F, Belhouchet M, Aldrovandi N, Tao S, Chen B, Liang G, Tesh RB, de Micco P, de Lamballerie X (2005b) Yunnan orbivirus, a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China. J Gen Virol 86:3409–3417PubMedGoogle Scholar
  18. Attoui H, Jaafar FM, Belhouchet M, de Micco P, de Lamballerie X, Brussaard CP (2006) Micromonas pusilla reovirus: a new member of the family Reoviridae assigned to a novel proposed genus (Mimoreovirus). J Gen Virol 87:1375–1383PubMedGoogle Scholar
  19. Attoui H, Maan S, Anthony SJ, Mertens PPC (2009a) Bluetongue virus, other orbiviruses and other reoviruses: their relationships and taxonomy. In: Mellor PS, Baylis M, Mertens PPC (eds) Bluetongue monograph. Elsevier/Academic Press, London, pp 23–552Google Scholar
  20. Attoui H et al (2009b) Peruvian horse sickness virus and Yunnan orbivirus, isolated from vertebrates and mosquitoes in Peru and Australia. Virology 394:298–310PubMedGoogle Scholar
  21. Attoui H et al (eds) (2011) Reoviridae. Elsevier/Academic Press, LondonGoogle Scholar
  22. Batten CA, Maan S, Shaw AE, Maan NS, Mertens PP (2008) A European field strain of bluetongue virus derived from two parental vaccine strains by genome segment reassortment. Virus Res 137:56–63PubMedGoogle Scholar
  23. Batten CA, van Rijn PA, Oura CA (2010) Detection of the European ‘field’ strain of bluetongue virus serotype 6 by real-time RT-PCR. Vet Microbiol 141:186–188PubMedGoogle Scholar
  24. Baylis M (2002) The re-emergence of bluetongue. Vet J 164:5–6PubMedGoogle Scholar
  25. Belaganahalli MN (2012) Full genome sequencing and phylogenetic analyses of different Orbivirus species. University of London, LondonGoogle Scholar
  26. Belaganahalli MN, Maan S, Maan NS, Tesh R, Attoui H, Mertens PP (2011) Umatilla virus genome sequencing and phylogenetic analysis: identification of stretch lagoon orbivirus as a new member of the Umatilla virus species. PLoS One 6:e23605PubMedCentralPubMedGoogle Scholar
  27. Belaganahalli MN, Maan S, Maan NS, Nomikou K, Pritchard I, Lunt R, Kirkland PD, Attoui H, Brownlie J, Mertens PP (2012) Full genome sequencing and genetic characterization of Eubenangee viruses identify Pata virus as a distinct species within the genus Orbivirus. PLoS One 7:e31911PubMedCentralPubMedGoogle Scholar
  28. Belhouchet M, Mohd Jaafar F, Tesh R, Grimes J, Maan S, Mertens PP, Attoui H (2010) Complete sequence of Great Island virus and comparison with the T2 and outer-capsid proteins of Kemerovo, Lipovnik and Tribec viruses (genus Orbivirus, family Reoviridae). J Gen Virol 91:2985–2993PubMedGoogle Scholar
  29. Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PPC, Attoui H (2011a) Detection of a fourth orbivirus non-structural protein. PLoS One 6(10):e25697. doi: 10.1371/journal.pone.0025697 PubMedCentralPubMedGoogle Scholar
  30. Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PP, Attoui H (2011b) Detection of a fourth orbivirus non-structural protein. PLoS One 6:e25697PubMedCentralPubMedGoogle Scholar
  31. Billinis C, Koumbati M, Spyrou V, Nomikou K, Mangana O, Panagiotidis CA, Papadopoulos O (2001) Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-PCR: a comparison with conventional methods. J Virol Methods 98:77–89PubMedGoogle Scholar
  32. Borden EC, Shope RE, Murphy FA (1971) Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus – a new taxonomic group. Physicochemical and serological studies. J Gen Virol 13:261–271PubMedGoogle Scholar
  33. Brown SE, Gonzalez HA, Bodkin DK, Tesh RB, Knudson DL (1988a) Intra- and inter-serogroup genetic relatedness of orbiviruses. II. Blot hybridization and reassortment in vitro of epizootic haemorrhagic disease serogroup, bluetongue type 10 and Pata viruses. J Gen Virol 69:135–147PubMedGoogle Scholar
  34. Brown SE, Morrison HG, Buckley SM, Shope RE, Knudson DL (1988b) Genetic relatedness of the Kemerovo serogroup viruses: I. RNA-RNA blot hybridization and gene reassortment in vitro of the Kemerovo serocomplex. Acta Virol 32:369–378PubMedGoogle Scholar
  35. Brown SE, Morrison HG, Karabatsos N, Knudson DL (1991) Genetic relatedness of two new orbivirus serogroups: orungo and lebombo. J Gen Virol 72:1065–1072PubMedGoogle Scholar
  36. Brown SE, Gorman BM, Tesh RB, Knudson DL (1992) Isolation of bluetongue and epizootic hemorrhagic disease viruses from mosquitoes collected in Indonesia. Vet Microbiol 32:241–252PubMedGoogle Scholar
  37. Calisher CH, Childs JE, Field HE, Holmes KV, Schountz T (2006) Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev 19:531–545PubMedCentralPubMedGoogle Scholar
  38. Carpi G, Holmes EC, Kitchen A (2010) The evolutionary dynamics of bluetongue virus. J Mol Evol 70:583–592PubMedGoogle Scholar
  39. Clavijo A, Sun F, Lester T, Jasperson DC, Wilson WC (2010) An improved real-time polymerase chain reaction for the simultaneous detection of all serotypes of Epizootic hemorrhagic disease virus. J Vet Diagn Invest 22:588–593PubMedGoogle Scholar
  40. Cowled C, Melville L, Weir R, Walsh S, Hyatt A, Van Driel R, Davis S, Gubala A, Boyle D (2007) Genetic and epidemiological characterization of Middle Point orbivirus, a novel virus isolated from sentinel cattle in northern Australia. J Gen Virol 88:3413–3422PubMedGoogle Scholar
  41. Cowled C, Palacios G, Melville L, Weir R, Walsh S, Davis S, Gubala A, Lipkin WI, Briese T, Boyle D (2009) Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia. J Gen Virol 90:1433–1439PubMedGoogle Scholar
  42. Darpel KE (2007) The bluetongue virus ‘ruminant host – insect vector’ transmission cycle; the role of Culicoides saliva proteins in infection. University of London, LondonGoogle Scholar
  43. de Mattos CC, de Mattos CA, Osburn BI, Dangler CA, Chuang RY, Doi RH (1989) Recombinant DNA probe for serotype-specific identification of bluetongue virus 17. Am J Vet Res 50:536–541PubMedGoogle Scholar
  44. Della-Porta AJ, Parsonson IM, McPhee DA (1985) Problems in the interpretation of diagnostic tests due to cross-reactions between orbiviruses and broad serological responses in animals. Prog Clin Biol Res 178:445–453PubMedGoogle Scholar
  45. Dilcher M et al (2012) Genetic characterization of Tribec virus and Kemerovo virus, two tick-transmitted human-pathogenic Orbiviruses. Virology 423:68–76PubMedGoogle Scholar
  46. Firth AE (2008) Bioinformatic analysis suggests that the Orbivirus VP6 cistron encodes an overlapping gene. Virol J 5:48PubMedCentralPubMedGoogle Scholar
  47. French TJ, Inumaru S, Roy P (1989) Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells. J Virol 63:3270–3278PubMedCentralPubMedGoogle Scholar
  48. Fu H, Leake CJ, Mertens PP, Mellor PS (1999) The barriers to bluetongue virus infection, dissemination and transmission in the vector, Culicoides variipennis (Diptera: Ceratopogonidae). Arch Virol 144:747–761PubMedGoogle Scholar
  49. Gambles RM (1949) Bluetongue of sheep on Cyprus. J Comp Pathol 59:176–190Google Scholar
  50. Ghiasi H, Purdy MA, Roy P (1985) The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Res 3:181–190PubMedGoogle Scholar
  51. Gibbens N (2012) Schmallenberg virus: a novel viral disease in northern Europe. Vet Rec 170:58PubMedGoogle Scholar
  52. Gonzalez HA, Knudson DL (1987) Genetic relatedness of corriparta serogroup viruses. J Gen Virol 68:661–672PubMedGoogle Scholar
  53. Gonzalez HA, Knudson DL (1988) Intra- and inter-serogroup genetic relatedness of orbiviruses. I. Blot hybridization of viruses of Australian serogroups. J Gen Virol 69:125–134PubMedGoogle Scholar
  54. Gorman BM, Taylor J, Walker PJ (1983) Orbiviruses. In: Joklik WK (ed) The reoviridae. Plenum Press, New York/London, pp 287–357Google Scholar
  55. Gould AR (1987) The complete nucleotide sequence of bluetongue virus serotype 1 RNA3 and a comparison with other geographic serotypes from Australia, South Africa and the United States of America, and with other orbivirus isolates. Virus Res 7:169–183PubMedGoogle Scholar
  56. Gould AR (1988) The use of recombinant DNA probes to group and type orbiviruses. A comparison of Australian and South African isolates. Arch Virol 99:205–220PubMedGoogle Scholar
  57. Gould AR, Eaton BT (1990) The amino acid sequence of the outer coat protein VP2 of neutralizing monoclonal antibody-resistant, virulent and attenuated bluetongue viruses. Virus Res 17:161–172PubMedGoogle Scholar
  58. Gould EA, Higgs S (2009) Impact of climate change and other factors on emerging arbovirus diseases. Trans R Soc Trop Med Hyg 103:109–121PubMedCentralPubMedGoogle Scholar
  59. Gutsche T (1979) There was a man. Timmins, Cape Town, p 4Google Scholar
  60. Hemati B, Contreras V, Urien C, Bonneau M, Takamatsu HH, Mertens PP, Breard E, Sailleau C, Zientara S, Schwartz-Cornil I (2009) Bluetongue virus targets conventional dendritic cells in skin lymph. J Virol 83:8789–8799PubMedCentralPubMedGoogle Scholar
  61. Henning MM (ed) (1956) African horse sickness, perdesiekte, Pestis equorum. In: Animal diseases in South Africa, 3rd edn. Central News Agency, PretoriaGoogle Scholar
  62. Hoffmann B, Eschbaumer M, Beer M (2009) Real-time quantitative reverse transcription-PCR assays specifically detecting bluetongue virus serotypes 1, 6, and 8. J Clin Microbiol 47:2992–2994PubMedCentralPubMedGoogle Scholar
  63. Hofmann MA, Renzullo S, Mader M, Chaignat V, Worwa G, Thuer B (2008) Genetic characterization of toggenburg orbivirus, a new bluetongue virus, from goats, Switzerland. Emerg Infect Dis 14:1855–1861PubMedGoogle Scholar
  64. Hooper PT et al (1999) Epidemic of blindness in kangaroos–evidence of a viral aetiology. Aust Vet J 77:529–536PubMedGoogle Scholar
  65. Huismans H, Cloete M (1987) A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA. Virology 158:373–380PubMedGoogle Scholar
  66. Huismans H, Erasmus BJ (1981) Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort J Vet Res 48:51–58PubMedGoogle Scholar
  67. Huismans H, Bremer CW, Barber TL (1979) The nucleic acid and proteins of epizootic haemorrhagic disease virus. Onderstepoort J Vet Res 46:95–104PubMedGoogle Scholar
  68. Hutcheon D (1902) Malarial catarrhal fever of sheep. Vet Rec 14:629–633Google Scholar
  69. Jansen CC et al (2009) Arboviruses isolated from mosquitoes collected from urban and peri-urban areas of eastern Australia. J Am Mosq Control Assoc 25:272–278PubMedGoogle Scholar
  70. Jimenez-Clavero MA et al (2006) High throughput detection of bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction: application on clinical samples from current Mediterranean outbreaks. J Vet Diagn Invest 18:7–17PubMedGoogle Scholar
  71. Karabatsos N (1985) International catalogue of arboviruses including certain other viruses of vertebrates. American Society of Tropical Medicine and Hygiene, San AntonioGoogle Scholar
  72. Katz JB, Alstad AD, Gustafson GA, Moser KM (1993) Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid. J Clin Microbiol 31:3028–3030PubMedCentralPubMedGoogle Scholar
  73. King A, Lefkowitz E, Adams MJ, Carstens EB (2011) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, London.Google Scholar
  74. Kirkland P (2005) Epidemic viral diseases of wildlife – sudden death in tammar wallabies, blind kangaroos, herpesviruses in pilchards-what next? Microbiol Aust 26(2):82–84Google Scholar
  75. Koekemoer JJ, Dijk AA (2004) African horsesickness virus serotyping and identification of multiple co-infecting serotypes with a single genome segment 2 RT-PCR amplification and reverse line blot hybridization. J Virol Methods 122:49–56PubMedGoogle Scholar
  76. Kowalik TF, Li JK (1989) Sequence analyses and structural predictions of double-stranded RNA segment S1 and VP7 from United States prototype bluetongue virus serotypes 13 and 10. Virology 172:189–195PubMedGoogle Scholar
  77. Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PP (2007a) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. J Gen Virol 88:621–630PubMedGoogle Scholar
  78. Maan S, Rao S, Maan NS, Anthony SJ, Attoui H, Samuel AR, Mertens PP (2007b) Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses. J Virol Methods 143:132–139PubMedGoogle Scholar
  79. Maan S et al (2008) Sequence analysis of bluetongue virus serotype 8 from the Netherlands 2006 and comparison to other European strains. Virology 377:308–318PubMedGoogle Scholar
  80. Maan S, Maan NS, Nomikou K, Anthony SJ, Ross-smith N, Singh KP, Samuel AR, Shaw AE, Mertens PPC (2009) Molecular epidemiology studies of bluetongue virus. In: Mellor PS, Baylis M, Mertens PPC (eds) Bluetongue. Elsevier/Academic Press, London, pp 135–166Google Scholar
  81. Maan NS et al (2010a) RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America. PLoS One 5:e12782PubMedCentralPubMedGoogle Scholar
  82. Maan S et al (2010b) Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains. PLoS One 5:e10323PubMedCentralPubMedGoogle Scholar
  83. Maan NS, Maan S, Nomikou K, Belaganahalli MN, Bachanek-Bankowska K, Mertens PP (2011a) Serotype specific primers and gel-based rt-PCR assays for ‘typing’ African horse sickness virus: identification of strains from Africa. PLoS One 6:e25686PubMedCentralPubMedGoogle Scholar
  84. Maan S, Maan NS, Nomikou K, Veronesi E, Bachanek-Bankowska K, Belaganahalli MN, Attoui H, Mertens PP (2011b) Complete genome characterisation of a novel 26th bluetongue virus serotype from Kuwait. PLoS One 6:e26147PubMedCentralPubMedGoogle Scholar
  85. Maan S et al (2011c) Novel bluetongue virus serotype from Kuwait. Emerg Infect Dis 17:886–889PubMedGoogle Scholar
  86. Maan NS, Maan S, Belaganahalli MN, Ostlund EN, Johnson DJ, Nomikou K, Mertens PPC (2012) Identification and differentiation of the twenty six bluetongue virus serotypes by RT–PCR amplification of the serotype-specific genome segment 2. PLoS One 7:e32601PubMedCentralPubMedGoogle Scholar
  87. Maclachlan NJ (2010) Global implications of the recent emergence of bluetongue virus in Europe. Vet Clin North Am Food Anim Pract 26:163–171PubMedGoogle Scholar
  88. Maclachlan NJ, Guthrie AJ (2010) Re-emergence of bluetongue, African horse sickness, and other orbivirus diseases. Vet Res 41:35PubMedGoogle Scholar
  89. MacLachlan NJ, Nunamaker RA, Katz JB, Sawyer MM, Akita GY, Osburn BI, Tabachnick WJ (1994) Detection of bluetongue virus in the blood of inoculated calves: comparison of virus isolation, PCR assay, and in vitro feeding of Culicoides variipennis. Arch Virol 136:1–8PubMedGoogle Scholar
  90. Mardis ER (2008) Next-generation DNA sequencing methods. Annu Rev Genomics Hum Genet 9:387–402PubMedGoogle Scholar
  91. Marr JS, Malloy CD (1996) An epidemiologic analysis of the ten plagues of Egypt. Caduceus 12:7–24PubMedGoogle Scholar
  92. Martins LC, Diniz JA, Silva EV, Barros VL, Monteiro HA, Azevedo RS, Quaresma JA, Vasconcelos PF (2007) Characterization of Minacu virus (Reoviridae: Orbivirus) and pathological changes in experimentally infected newborn mice. Int J Exp Pathol 88:63–73PubMedCentralPubMedGoogle Scholar
  93. Mellor PS, Hamblin C (2004) African horse sickness. Vet Res 35:445–466PubMedGoogle Scholar
  94. Mertens P (2004) The dsRNA viruses. Virus Res 101:3–13PubMedGoogle Scholar
  95. Mertens P, Attoui H (2009) Orbiviruses and bluetongue virus. In: Encyclopedia of life sciences (ELS). Wiley, Chichester: John Wiley & Sons, LtdGoogle Scholar
  96. Mertens PP, Diprose J (2004) The bluetongue virus core: a nano-scale transcription machine. Virus Res 101:29–43PubMedGoogle Scholar
  97. Mertens PP, Sangar DV (1985) Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140:55–67PubMedGoogle Scholar
  98. Mertens P, Attoui H (2009) Orbiviruses and bluetongue virus. Encyclopedia of life sciences (ELS). Wiley, ChichesterGoogle Scholar
  99. Mertens PP, Brown F, Sangar DV (1984) Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217PubMedGoogle Scholar
  100. Mertens PP, Burroughs JN, Anderson J (1987) Purification and properties of virus particles, infectious subviral particles, and cores of bluetongue virus serotypes 1 and 4. Virology 157:375–386PubMedGoogle Scholar
  101. Mertens PP, Pedley S, Cowley J, Burroughs JN, Corteyn AH, Jeggo MH, Jennings DM, Gorman BM (1989) Analysis of the roles of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype. Virology 170:561–565PubMedGoogle Scholar
  102. Mertens PP, Burroughs JN, Walton A, Wellby MP, Fu H, O’Hara RS, Brookes SM, Mellor PS (1996) Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species. Virology 217:582–593PubMedGoogle Scholar
  103. Mertens PPC, Maan S, Samuel A, Attoui H (2005a) Orbiviruses, reoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (ed) Virus taxonomy. Eighth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, pp 466–483Google Scholar
  104. Mertens PPC, Attoui H, Duncan R, Dermody TS (2005b) Reoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (ed) Virus taxonomy. Eighth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, LondonGoogle Scholar
  105. Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S (2007) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol 88:2811–2823PubMedGoogle Scholar
  106. Mohammed ME, Aradaib IE, Mukhtar MM, Ghalib HW, Riemann HP, Oyejide A, Osburn BI (1996) Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan. Vet Microbiol 52:201–208PubMedGoogle Scholar
  107. Mohd Jaafar F, Attoui H, Bahar MW, Siebold C, Sutton G, Mertens PP, De Micco P, Stuart DI, Grimes JM, De Lamballerie X (2005) The structure and function of the outer coat protein VP9 of Banna virus. Structure 13:17–28PubMedGoogle Scholar
  108. Monaco F, Polci A, Lelli R, Pinoni C, Di Mattia T, Mbulu RS, Scacchia M, Savini G (2011) A new duplex real-time RT-PCR assay for sensitive and specific detection of African horse sickness virus. Mol Cell Probes 25:87–93PubMedGoogle Scholar
  109. Moore DL (1974) Bluetongue and related viruses in Ibadan, Nigeria: serologic comparison of bluetongue, epizootic hemorrhagic disease of deer, and Abadina (Palyam) viral isolates. Am J Vet Res 35:1109–1113PubMedGoogle Scholar
  110. Moore DL, Lee VH (1972) Antigenic relationship between the virus of epizootic haemorrhagic disease of deer and bluetongue virus. Brief report. Arch Gesamte Virusforsch 37:282–284PubMedGoogle Scholar
  111. Moule L (1896) Histoire de la Médecine Vétérinaire. Presses universitaires de France, Maulde, Paris, p 38Google Scholar
  112. Murphy FA, Borden EC, Shope RE, Harrison A (1971) Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus – a new taxonomic group. Electron microscopic studies. J Gen Virol 13:273–288PubMedGoogle Scholar
  113. Niedringhaus TP, Milanova D, Kerby MB, Snyder MP, Barron AE (2011) Landscape of next-generation sequencing technologies. Anal Chem 83:4327–4341PubMedCentralPubMedGoogle Scholar
  114. Nomikou K, Dovas CI, Maan S, Anthony SJ, Samuel AR, Papanastassopoulou M, Maan NS, Mangana O, Mertens PP (2009) Evolution and phylogenetic analysis of full-length VP3 genes of Eastern Mediterranean bluetongue virus isolates. PLoS One 4:e6437PubMedCentralPubMedGoogle Scholar
  115. OIE (2010) Chapter 1.2. Criteria for listing diseases. Terrestrial Animal Health Code. http://www.oie.int/index.php?id=169&L=0&htmfile=chapitre_1.1.2.htm. Accessed 15 Nov 2013
  116. Orru G, Ferrando ML, Meloni M, Liciardi M, Savini G, De Santis P (2006) Rapid detection and quantitation of Bluetongue virus (BTV) using a Molecular Beacon fluorescent probe assay. J Virol Methods 137:34–42PubMedGoogle Scholar
  117. Oura C (2011) Bluetongue: new insights and lessons learnt. Vet Rec 168:375–376PubMedGoogle Scholar
  118. Ozkul A, Erturk A, Caliskan E, Sarac F, Ceylan C, Mertens P, Kabakli O, Dincer E, Cizmeci SG (2009) Segment 10 based molecular epidemiology of bluetongue virus (BTV) isolates from Turkey: 1999–2001. Virus Res 142:134–139PubMedGoogle Scholar
  119. Palacios G et al (2011) Rapid molecular strategy for orbivirus detection and characterization. J Clin Microbiol 49:2314–2317PubMedCentralPubMedGoogle Scholar
  120. Parkes H, Gould AR (1996) Characterisation of Wongorr virus, an Australian orbivirus. Virus Res 44:111–122PubMedGoogle Scholar
  121. Polydorou K (ed) (1985) Bluetongue in Cyprus. Alan R Liss Inc., New YorkGoogle Scholar
  122. Potgieter AC, Steele AD, van Dijk AA (2002) Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes. J Gen Virol 83:2215–2223PubMedGoogle Scholar
  123. Purdy MA, Ghiasi H, Rao CD, Roy P (1985) Complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies. J Virol 55:826–830PubMedCentralPubMedGoogle Scholar
  124. Quan M, Lourens CW, MacLachlan NJ, Gardner IA, Guthrie AJ (2010) Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus. J Virol Methods 167:45–52PubMedGoogle Scholar
  125. Rafyi A (1961) Horse sickness. Bull Off Int Epizoot 56:216–250Google Scholar
  126. Rao CD, Kiuchi A, Roy P (1983) Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus. J Virol 46:378–383PubMedCentralPubMedGoogle Scholar
  127. Ratinier M et al (2011) Identification and characterization of a novel non-structural protein of bluetongue virus. PLoS Pathog 7:e1002477PubMedCentralPubMedGoogle Scholar
  128. Rodriguez-Sanchez B, Fernandez-Pinero J, Sailleau C, Zientara S, Belak S, Arias M, Sanchez-Vizcaino JM (2008) Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus. J Virol Methods 151:87–94PubMedGoogle Scholar
  129. Rose KA, Kirkland PD, Davis RJ, Cooper DW, Blumstein DT (2000) An epizootic of sudden death in tammar wallabies (Macropus eugenii). American Association of Zoo Veterinarians and International Association for Aquatic Animal Medicine Annual Conference Proceeding, pp 166–170Google Scholar
  130. Roy P (1989) Bluetongue virus genetics and genome structure. Virus Res 13:179–206PubMedGoogle Scholar
  131. Roy P, Purdy MA, Petre J, Rao CD (1985a) Cloning and nucleotide sequencing of bluetongue virus genomes. Prog Clin Biol Res 178:363–370PubMedGoogle Scholar
  132. Roy P, Ritter GD Jr, Akashi H, Collisson E, Inaba Y (1985b) A genetic probe for identifying bluetongue virus infections in vivo and in vitro. J Gen Virol 66:1613–1619PubMedGoogle Scholar
  133. Shapiro A, Green T, Rao S, White S, Carner G, Mertens PP, Becnel JJ (2005) Morphological and molecular characterization of a Cypovirus (Reoviridae) from the mosquito Uranotaenia sapphirina (Diptera: Culicidae). J Virol 79:9430–9438PubMedCentralPubMedGoogle Scholar
  134. Shaw AE et al (2007) Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods 145:115–126PubMedGoogle Scholar
  135. Shope RE (1955) An infectious fibroma of deer. Proc Soc Exp Biol Med 88:533–535PubMedGoogle Scholar
  136. Shope RE, MacNamara LG, Mangold R (1955) Epizootic hemorrhagic disease of deer. New Jersey Outdoors (November). Report. pp 16–21Google Scholar
  137. Spreull J (1902) Report from veterinary surgeon Spreull on the result of his experiments with the malarial catarrhal fever of sheep. Agric J Cape Good Hope 20:469–477Google Scholar
  138. Spreull J (1905) Malarial catarrhal fever (bluetongue) of sheep in South Africa. J Comp Pathol 18:321–337Google Scholar
  139. Squire KR, Chuang RY, Chuang LC, Doi RH, Osburn BI (1985a) Molecular cloning and hybridization studies on bluetongue virus serotype 17. Prog Clin Biol Res 178:355–361PubMedGoogle Scholar
  140. Squire KR, Chuang RY, Chuang LF, Doi RH, Osburn BI (1985b) Detecting bluetongue virus RNA in cell culture by dot hybridization with a cloned genetic probe. J Virol Methods 10:59–68PubMedGoogle Scholar
  141. Stone-Marschat M, Carville A, Skowronek A, Laegreid WW (1994) Detection of African horse sickness virus by reverse transcription-PCR. J Clin Microbiol 32:697–700PubMedCentralPubMedGoogle Scholar
  142. Tabachnick WJ, MacLachlan NJ, Thompson LH, Hunt GJ, Patton JF (1996) Susceptibility of Culicoides variipennis sonorensis to infection by polymerase chain reaction-detectable bluetongue virus in cattle blood. Am J Trop Med Hyg 54:481–485PubMedGoogle Scholar
  143. Takamatsu H, Mellor PS, Mertens PP, Kirkham PA, Burroughs JN, Parkhouse RM (2003) A possible overwintering mechanism for bluetongue virus in the absence of the insect vector. J Gen Virol 84:227–235PubMedGoogle Scholar
  144. Theal GM (1899) Records of South-Eastern Africa collected in various libraries and archive departments in Europe. Government of Cape Colony, Cape Town, South Africa Report no. 3, p 224Google Scholar
  145. Tomori O, Fabiyi A (1976) Neutralizing antibodies to Orungo virus in man and animals in Nigeria. Trop Geogr Med 28:233–238PubMedGoogle Scholar
  146. Toussaint JF, Sailleau C, Breard E, Zientara S, De Clercq K (2007) Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J Virol Methods 140:115–123PubMedGoogle Scholar
  147. van Regenmortel MH, Mahy BW (2004) Emerging issues in virus taxonomy. Emerg Infect Dis 10:8–13PubMedGoogle Scholar
  148. Venter EH, Viljoen GJ, Nel LH, Huismans H, van Dijk AA (1991) A comparison of different genomic probes in the detection of virus-specified RNA in Orbivirus-infected cells. J Virol Methods 32:171–180PubMedGoogle Scholar
  149. Verwoerd DW, Louw H, Oellermann RA (1970) Characterization of bluetongue virus ribonucleic acid. J Virol 5:1–7PubMedCentralPubMedGoogle Scholar
  150. Victoria JG, Kapoor A, Dupuis K, Schnurr DP, Delwart EL (2008) Rapid identification of known and new RNA viruses from animal tissues. PLoS Pathog 4:e1000163PubMedCentralPubMedGoogle Scholar
  151. Vieira Cde M, Nunes MR, da Silva EV, Carvalho VL, Nunes Neto JP, Cruz AC, Casseb SM, Vasconcelos HB, Quaresma JA, Vasconcelos PF (2009) Full-length sequencing and genetic characterization of Breu Branco virus (Reoviridae, Orbivirus) and two related strains isolated from Anopheles mosquitoes. J Gen Virol 90:2183–2190PubMedGoogle Scholar
  152. Wade-Evans AM (1990) The complete nucleotide sequence of genome segment 7 of bluetongue virus, serotype 1 from South Africa. Nucleic Acids Res 18:4919PubMedCentralPubMedGoogle Scholar
  153. Wade-Evans AM, Mertens PP, Bostock CJ (1990) Development of the polymerase chain reaction for the detection of bluetongue virus in tissue samples. J Virol Methods 30:15–24PubMedGoogle Scholar
  154. Wade-Evans AM, Mertens PP, Belsham GJ (1992) Sequence of genome segment 9 of bluetongue virus (serotype 1, South Africa) and expression analysis demonstrating that different forms of VP6 are derived from initiation of protein synthesis at two distinct sites. J Gen Virol 73:3023–3026PubMedGoogle Scholar
  155. Weaver SC, Reisen WK (2010) Present and future arboviral threats. Antiviral Res 85:328–345PubMedCentralPubMedGoogle Scholar
  156. Wilson A, Carpenter S, Gloster J, Mellor P (2007) Re-emergence of bluetongue in northern Europe in 2007. Vet Rec 161:487–489PubMedGoogle Scholar
  157. Wilson WC, O’Hearn ES, Tellgren-Roth C, Stallknecht DE, Mead DG, Mecham JO (2009a) Detection of all eight serotypes of Epizootic hemorrhagic disease virus by real-time reverse transcription polymerase chain reaction. J Vet Diagn Invest 21:220–225PubMedGoogle Scholar
  158. Wilson WC, Hindson BJ, O’Hearn ES, Hall S, Tellgren-Roth C, Torres C, Naraghi-Arani P, Mecham JO, Lenhoff RJ (2009b) A multiplex real-time reverse transcription polymerase chain reaction assay for detection and differentiation of Bluetongue virus and Epizootic hemorrhagic disease virus serogroups. J Vet Diagn Invest 21:760–770PubMedGoogle Scholar
  159. Wu X, Chen SY, Iwata H, Compans RW, Roy P (1992) Multiple glycoproteins synthesized by the smallest RNA segment (S10) of bluetongue virus. J Virol 66:7104–7112PubMedCentralPubMedGoogle Scholar
  160. Zientara S, Sailleau C, Moulay S, Plateau E, Cruciere C (1993) Diagnosis and molecular epidemiology of the African horse sickness virus by the polymerase chain reaction and restriction patterns. Vet Res 24:385–395PubMedGoogle Scholar
  161. Zientara S, Sailleau C, Moulay S, Cruciere C, El-Harrak M, Laegreid WW, Hamblin C (1998) Use of reverse transcriptase-polymerase chain reaction (RT-PCR) and dot-blot hybridisation for the detection and identification of African horse sickness virus nucleic acids. Arch Virol Suppl 14:317–327PubMedGoogle Scholar
  162. Zientara S et al (2002) Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome. Vet Rec 150:598–601PubMedGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  • Sushila Maan
    • 1
  • Manjunatha N. Belaganahalli
    • 2
  • Narender S. Maan
    • 1
  • Peter P. C. Mertens
    • 3
  1. 1.LLR University of Veterinary and Animal SciencesHisarIndia
  2. 2.Arbovirus Molecular Research GroupInstitute for Animal Health, Pirbright LaboratoryWoking, SurreyUK
  3. 3.Vector-borne Viral Diseases (VVD) ProgrammeInstitute for Animal Health, Pirbright LaboratoryWoking, SurreyUK

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