Whole Genome Sequencing Strategies and Development of Orbivirus Sequence Database: Implications for Novel dsRNA Virus Detection

  • Sushila Maan
  • Manjunatha N. Belaganahalli
  • Narender S. Maan
  • Peter P. C. Mertens


The genus Orbivirus is the largest of the genera within the family Reoviridae, containing 22 recognised virus species as well as 15 unclassified ‘orbiviruses’, which could potentially represent further new species. The orbiviruses are transmitted by both ticks and/or haematophagous insect vectors. They have a wide host range that includes domestic and wild ruminants, equines, marsupials, sloths, bats, birds and humans. Low-level serological cross-reactions between different species of orbiviruses and lack of reference strains/antisera for existing Orbivirus species make serological identification of new virus isolates difficult. Recently, whole genome sequence data (WGS) has become an important tool for the detection, classification and epidemiological investigations of different pathogens. This study presents full genome sequence database of all known 22 Orbivirus species (including 5 unclassified viruses). Development of novel sequencing strategies and phylogenetic analysis of the orbiviruses using this database has identified five novel Orbivirus species and has facilitated development of a pan-orbivirus RT-PCR assay that can be used to identify the RNA of any Orbivirus species. These techniques will support Orbivirus discovery with greater accuracy than before and can be used for definitive diagnosis of suspected Orbivirus infection.


Genome Segment Wide Host Range Virus Species dsRNA Virus Wild Ruminant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This paper is based on a presentation made at the International conference on Biotechnology: Emerging Trends (ICB-2012) organized by Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, Haryana, India from 18-20th September 2012. I would like to thank the organisers for inviting me to the meeting. We are extremely grateful to the many colleagues at IAH Pirbright, who had their direct or indirect contributions to these studies.


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Copyright information

© Springer India 2013

Authors and Affiliations

  • Sushila Maan
    • 1
  • Manjunatha N. Belaganahalli
    • 2
  • Narender S. Maan
    • 1
  • Peter P. C. Mertens
    • 3
  1. 1.LLR University of Veterinary and Animal SciencesHisarIndia
  2. 2.Arbovirus Molecular Research GroupInstitute for Animal Health, Pirbright LaboratoryWoking, SurreyUK
  3. 3.Vector-borne Viral Diseases (VVD) ProgrammeInstitute for Animal Health, Pirbright LaboratoryWoking, SurreyUK

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