Abstract
Proteins are macromolecules and the main components of the physiological metabolic pathways of cells. The post-genomic era focuses on the complete understanding of the structure and functions of proteins. Proteome, the protein complement of a given cell at a specific time is the main focus of proteomics. Proteomics has recently been of interest to scientists because it gives a much better understanding of an organism than genomics. A number of advanced techniques such as 2D-gel electrophoresis, HPLC, Mass spectrometry, MALDI-TOF, NMR, DNA microarray, etc., are used for the study of proteomics. The most important applications of proteomics are in the discovery of biomarkers and drug targets. It will also be useful in diseases which involve multigenes, e.g., cancer, diabetes II, asthma, hypertension, cardiovascular and neurological diseases. This chapter focuses on the basic steps involved, techniques used, and applications of proteomics.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Andersen JS, Svensson B, Roepstorff P (1996) Electrospray ionization and matrix assisted laser desorption ionization mass spectrometry: powerful analytical tools in recombinant protein chemistry. Nat Biotechnol 14:449–457
Binz PA, Gasteiger E, Sanchez J, Bairoch A, Hochstrasser DF, Appel, RD (2004) Proteome analysis. In: Lengauer Th (ed) Bioinformatics. from Genomes to Drugs. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. ISBN:3-527-29988-2, pp 69–118
Cagney G, Amiri S, Premawaradena T, Lindo M, Emili A (2003). In silico proteome analysis to facilitate proteomics experiments using mass spectrometry. Proteome Science 1:5. http://www.proteomesci.com/content/1/1/5 (Open Access article)
Garret RH, Grisham CM (1995) Proteins: their biological functions and primary structure. In: Biochemistry Gareet RH, Grisham CM (eds) Saunders College Publishing, Harcourt Brace College Publishers, Philadelphia, pp 81–131
Graves PR, Haystead AJ (2002) Molecular biologists guide to proteomics. Microbiol Mol Biol Rev 66(1):39–63
Hillenkamp F, Karas M, Beavis RC, Chait BT (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal Chem 63(24):1193A–1203A
MacBeath G, Schreiber S (2000) Printing proteins as microarrays for high-throughput function determination. Science 289(5485):1760–1764
Okerberg ES, Wu J, Zhang B, Sammi B, Blackford K, Winn DT, Shreder KR, Burbaum JJ, Palricelli MP (2005) High resolution functional proteomics by active site peptide profiling. PNAS 102(14):4996–5001
Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270(5235):467–470
Yates JR, Ruse CI, Nakorchevesky A (2009) Proteomics by mass spectrometry: approaches, advances and applications. Annu Rev Biomed Eng 11:49–79
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer India
About this chapter
Cite this chapter
Ravi, I. (2014). Proteomics. In: Ravi, I., Baunthiyal, M., Saxena, J. (eds) Advances in Biotechnology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1554-7_8
Download citation
DOI: https://doi.org/10.1007/978-81-322-1554-7_8
Published:
Publisher Name: Springer, New Delhi
Print ISBN: 978-81-322-1553-0
Online ISBN: 978-81-322-1554-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)