DNA Sequencing: Methods and Applications

  • Satpal Singh Bisht
  • Amrita Kumari Panda


Determination of the precise order of nucleotides within a DNA molecule is popularly known as DNA sequencing. About three decades ago in the year 1977, Sanger and Maxam–Gilbert made a breakthrough that revolutionized the world of biological sciences by sequencing the 5,386-base bacteriophage φX174. From the year 1977 to till date DNA sequencing came across much advancement in terms of sequencing tools and techniques. The modern era DNA sequencing are dealing with Next generation sequencing and many other advancement are available to the researchers, practitioners, and academicians at a very reasonable cost with highest accuracy. The biological databases are being flooded with a huge flow of sequences coming out from various organisms across the world. Today the researchers and scientists across the various fields are utilizing these data for a variety of applications including food security by developing better crops and crop yields, livestock, improved diagnostics, prognostics, and therapies for many complex diseases.


Massively Parallel Signature Sequencing Genome Shotgun Dimethyl Sulfate Illumina Genome Analyzer Ribose Sugar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Dale JW, Schantz MV (2007) DNA sequencing. In: from genes to genomes concepts and applications of DNA technology, 2nd edn. Wiley Ltd, West Sussex, EnglandGoogle Scholar
  2. Drmanac R et al (2010) Human genome sequencing using unchained base reads in self-assembling DNA nanoarrays. Science 327(5961):78–81. doi: 10.1126/science.1181498 PubMedCrossRefGoogle Scholar
  3. Edwards JR, Ruparel H, Ju J (2005) Mass-spectrometry DNA sequencing. Mutat Res 573(1–2):3–12. doi: org/10.1016/j.mrfmmm.2004.07.021 PubMedCrossRefGoogle Scholar
  4. Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer races using Phred. I. Accuracy assessment. Genome Res 8:175–185. doi: 10.1101/gr.8.3.175 PubMedCrossRefGoogle Scholar
  5. Haas BJ, Chin M, Nusbaum C, Birren BW, Livny J (2012) How deep is deep enough for RNA-Seq profiling of bacterial transcriptomes? BMC Genomics 13: 734Google Scholar
  6. Liu L, Li Y, Li S, Hu N, He Y, Pong R, Lin D, Lu L, Law M (2012) Comparison of Next-Generation Sequencing Systems. J Biomed Biotechnol 2012:1–11. doi: 10.1155/2012/251364
  7. Loman NJ, Misra RV, Dallman TJ, Constantinidou C, Gharbia SE, Wain J, Pallen MJ (2012) Performance comparison of benchtop high-throughput sequencing platforms. Nat Biotechnol 30: 434–439. doi: 10.1038/nbt.2198 Google Scholar
  8. Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Nat Acad Sci 74(2):560–564. PMCID: PMC392330. doi: 10.1073/pnas.74.2.560
  9. Nakamura K, Oshima T, Morimoto T, Ikeda S, Yoshikawa H, Shiwa Y, Ishikawa S, Linak MC, Hirai A, Takahashi H, Altaf-Ul-Amin M, Ogasawara N, Kanaya S (2011) Sequence-specific error profile of Illumina sequencers. Nucleic Acids Res 39(13). doi: 10.1093/nar/gkr34410.1093/nar/gkr344
  10. Pettersson E, Lundeberg J, Ahmadian A (2009) Generations of sequencing technologies. Genomics 93(2):105–11. 10.1016/j.ygeno.2008.10.003 Google Scholar
  11. Porreca JG (2010) Genome sequencing on nanoballs. Nat Biotechnol 28(1):43–44. doi: 10.1038/nbt0110-43 PubMedCrossRefGoogle Scholar
  12. Quail MA, Smith M, Coupland P, Otto TD, Harris SR, Connor TR, Bertoni A, Swerdlow HP, Gu Y (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 13:341Google Scholar
  13. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Nat Acad Sci 74(12):54635467. doi: 10.1073/pnas.74.12.5463 CrossRefGoogle Scholar
  14. Schuster SC (2008) Next-generation sequencing transforms today’s biology. Nat Methods 5(1):16–18. doi: 10.1038/nmeth1156 PubMedCrossRefGoogle Scholar
  15. Xiaoguang Z, Lufeng R, Yuntao L, Meng Z, Yude Y, Jun Y (2010) The next-generation sequencing technology: a technology review and future perspective. Sci China Life Sci 53(1):44–57. doi: 10.1007/s11427-010-0023-6 CrossRefGoogle Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Biotechnology School of Life SciencesMizoram UniversityAizawlIndia
  2. 2.BiotechnologyRoland Institute of Pharmaceutical SciencesBerhampurIndia

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