• Mayank
  • Rachana Arya


A biosensor is an analytical tool used for qualitative and quantitative detection of analyte. It incorporates a biological recognition element such as enzyme, microbial cell, receptor, DNA, antibody, antigen, etc., which interacts with the analyte to produce primary response. This initial response is identified and converted into a suitable electronic form with the help of a suitable transducer device. Selection of transducer is based on the type of signal obtained from the interaction of biological recognition element and analyte. The commonly used devices are electronic, optical, mechanical, and thermal transducer. To insure accurate response, suitable immobilization techniques are required for biological recognition elements, which provide proper interaction of transducer with the signal obtained from primary sensing. Adsorption, cross-linking, entrapment, and covalent binding are some commonly applied techniques for immobilization. The electronic signal obtained from transducer may easily be modified, amplified, displayed, and recorded by suitable electronic devices. The use of biological component for sensing makes the biosensor very specific, fast, and reliable, thus biosensors are nowadays utilized in various industries for monitoring such as food quality control, medical research, clinical diagnosis, environmental monitoring, agriculture, bioprocess monitoring and control, and pharmaceutical industry, etc.


Biological Oxygen Demand Biological Component Glucose Biosensor Immobilization Technique Membrane Transport Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Abel AP, Weller MG, Duveneck GL, Ehrat M, Widmer HM (1996) Fiber-optic evanescent wave biosensor for the detection of oligonucleotides. Anal Chem 68:2905–2912PubMedCrossRefGoogle Scholar
  2. Brooks SL, Higgins IJ, Newman JD, Turnerp APF (1991) Biosensors for process control. Enzyme Microbial Technol 13:946–955CrossRefGoogle Scholar
  3. Burlage R, Kuo CT (1994) Living biosensors for the management and manipulation of microbial consortia. Annu Rev Microbiol 48:291–309PubMedCrossRefGoogle Scholar
  4. Chee GI, Nomura Y, Karube I (1999) Biosensor for the estimation of low biochemical oxygen demand. Anal Chim Acta 379:85–191CrossRefGoogle Scholar
  5. Clark LC Jr (1956) Monitor and control of blood and tissue oxygen tensions. Trans Am Soc Artif Intern Organs 2:41–48Google Scholar
  6. Clark LC Jr, Lyons C (1962) Electrode systems for continuous monitoring cardiovascular surgery. Ann N Y Acad Sci 102:29–45PubMedCrossRefGoogle Scholar
  7. Deshpande A, D’Souza SF, Nadkarni GB (1986) Immobilization of microbial cells in gelatine using gamma-irradiation. Indian J Biochem Biophys 23:353–354PubMedGoogle Scholar
  8. Di Paolantonio CL, Rechnitz GA (1982) Induced bacterial electrode for the potentiometric measurement of tyrosine. Anal Chim Acta 148:1–12Google Scholar
  9. Di Paolantonio CL, Rechnitz GA (1983) Stabilized bacteria-based potentiometric electrode for pyruvate. Anal Chim Acta 148:1–12CrossRefGoogle Scholar
  10. Endo H, Kamata A, Hoshi M, Hayashi T, Watanabe E (1995) Microbial biosensor system for rapid determination of vitamin B-6. J Food Sci 60:554–557Google Scholar
  11. Fleschin S, Bala C, Bunaciu AA, Panait A, Aboul-Enein HY (1998) Enalapril microbial biosensor. Prep Biochem Biotechnol 28:261–269PubMedCrossRefGoogle Scholar
  12. Ikebukuro K, Honda M, Nakanishi K, Nomura Y, Masuda Y, Yokoyama K, Yamauchi Y, Karube I (1996) Flow-type cyanide sensor using an immobilized microorganism. Electroanalysis 8:876–879CrossRefGoogle Scholar
  13. Karube I, Wang Y, Tamiya E, Kawarai M (1987) Microbial electrode sensor for vitamin-B12. Anal Chim Acta 199:93–97CrossRefGoogle Scholar
  14. Katrlik J, Svorc J, Rosenberg M, Miertus S (1996) Whole cell amperometric biosensor based on Aspergillus niger for determination of glucose with enhanced upper linearity limit. Anal Chim Acta 331:225–232CrossRefGoogle Scholar
  15. Kiefer H, Klee B, John E, Stierhof YD, Jähnig F (1991) Biosensors based on membrane transport proteins. Biosens Bioelectron 6(3):233–237PubMedCrossRefGoogle Scholar
  16. Klibanov AM (1983) Stabilization of enzymes against thermal inactivation. Adv Appl Microbiol 29:1–28PubMedCrossRefGoogle Scholar
  17. Liu B, Cui Y, Deng J (1996) Studies on microbial biosensor for DL-phenylalanine and its dynamic response process. Anal Lett 29:1497–1515CrossRefGoogle Scholar
  18. Liu J, Bjornsson L, Mattiasson B (2000) Immobilized activated sludge based biosensor for biochemical oxygen demand measurement. Biosens Bioelectron 14:883–893PubMedCrossRefGoogle Scholar
  19. Mascini M, Memoli A, Olana F (1989) Microbial sensor for alcohol. Enzyme Microb Technol 11:297–301CrossRefGoogle Scholar
  20. Matsunaga T, Suzuki S, Tomoda R (1984) Photomicrobial sensor for selective determination of phosphate. Enzyme Microb Technol 6:355–357CrossRefGoogle Scholar
  21. Mehrvar M, Bis C, Scharer JM, Young MM, Luong JH (2000) Fiber-optic biosensors–trends and advances. Anal Sci 16:677–692CrossRefGoogle Scholar
  22. Mulchandani A, Mulchandani P, Kaneva I, Chen W (1998) Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophophorous hydrolase. 1. Potentiometric microbial electrode. Anal Chem 70:4140–4145PubMedCrossRefGoogle Scholar
  23. Nandakumar R, Mattiasson B (1999) A microbial biosensor using Psuedomonas putida cells immobilized in an expanded bed reactors for the on-line monitoring of phenolic compounds. Anal Lett 32:2379–2393CrossRefGoogle Scholar
  24. Nomura Y, Ikebukuno K, Yokoyama K, Takeuchi T, Arikawa Y, Ohno S, Karube I (1994) A novel microbial sensor for anionic surfactant determination. Anal Lett 27:3095–3108CrossRefGoogle Scholar
  25. Renneberg R, Riedel K, Scheller F (1985) Microbial sensor for aspartame. Appl Microbiol Biotechnol 21:180–181CrossRefGoogle Scholar
  26. Reshetilov AN, Iliasov PV, Knackmuss HJ, Boronin AM (2000) The nitrite oxidising activity of Nitrobacter strains as a base of microbial biosensor for nitrite detection. Anal Lett 33:29–41CrossRefGoogle Scholar
  27. Riedel K (1998) Microbial biosensors based on oxygen electrodes. In: Mulchandani A, Rogers KR (eds) Enzyme and Microbial Biosensors: techniques and protocols. Humanae Press, Totowa, pp 199–223CrossRefGoogle Scholar
  28. Riedel K, Hensel J, Rothe S, Neumann B, Scheller F (1993) Microbial sensor for determination of aromatics and their chloroderivatives. Part-II: determination of chlorinated phenols using a Rhodococcus containing biosensor. Appl Microbiol Biotechnol 38:556–559CrossRefGoogle Scholar
  29. Riedel K, Beyersdorf R, Neumann B, Scheller F (1995) Microbial sensor for determination of aromatics and their chloroderivatives. Part-III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelli (cutaneum). Appl Microbiol Biotechnol 43:7–9CrossRefGoogle Scholar
  30. Scouten WH (1987) A survey of enzyme coupling techniques. Methods Enzymol 135:30–65PubMedCrossRefGoogle Scholar
  31. Sochaczewski EP, Luong JHT, Guilbault GG (1990) Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium. Enzyme Microb Technol 12:173–183CrossRefGoogle Scholar
  32. Suzuki S, Karube I (1987) An amperometric sensor for carbon dioxide based on immobilized bacteria utilizing carbondioxide. Anal Chim Acta 199:85–91CrossRefGoogle Scholar
  33. Svitel J, Curilla O, Tkac J (1998) Microbial cell-based biosensorfor sensing glucose, sucrose or lactose. Biotechnol Appl Biochem 27:153–158PubMedGoogle Scholar
  34. Tag K, Lehmann M, Chan C, Renneberg R, Riedel K, Kunze G (2000) Measurement of biodegradable substances with a mycelia-sensor based on the salt tolerant yeast Arxula adeninivorans LS3. Sens Actuators B 67:142–148CrossRefGoogle Scholar
  35. Ukeda H, Wagner G, Bilitewski U, Schmid RD (1992a) Flow injection analysis of short-chain fatty acids in milk based on a microbial electrode. J Agric Food Chem 40:2324–2327CrossRefGoogle Scholar
  36. Ukeda H, Wagner G, Weis G, Miller M, Klostermeyer H, Schmid RD (1992b) Application of a microbial sensor for determination of short-chain fatty acids in raw milk samples. Z. Lebensm Unters Forsch 195:1–2PubMedCrossRefGoogle Scholar
  37. Updike SJ, Hicks GP (1967) The enzyme electrode. Nature 214:986–988PubMedCrossRefGoogle Scholar
  38. Yang Z, Suzuki H, Sasaki S, Karube I (1996) Disposable sensor for biochemical oxygen demand. Appl Microbiol Biotechnol 46:10–14PubMedCrossRefGoogle Scholar
  39. Zhang X, Teng Y, Fu Y, Xu L, Zhang S, He B, Wang C, Zhang W (2010) Lectin-based biosensor strategy for electrochemical assay of glycan expression on living cancer cells. Anal Chem 82(22):9455–9460PubMedCrossRefGoogle Scholar

Copyright information

© Springer India 2014

Authors and Affiliations

  1. 1.Biochemical Engineering DepartmentBT Kumaon Institute of TechnologyDwarahatIndia
  2. 2.Electronics and Communication Engineering DepartmentBT Kumaon Institute of TechnologyDwarahatIndia

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