DNA as a master molecule in the cell has generally been regarded as static, with the only movement associated being at the time of chromatid/chromosome separation during cell division. This notion stayed for a long time, and, in fact, allowed geneticists to generate extensive linkage maps wherein a gene was assigned a fixed location on the chromosome. Such maps have served many useful purposes in genetic analyses in all organisms in the years to come, and still constitute an important exercise in genetics. However, in the 1940s Barbara McClintock described genetic elements in maize, which had the capacity to move around the genome. Calling them “controlling elements”, McClintock was able to show that their mobility greatly influences the gene expression.
Insertion Sequence Target Site Duplication Strand Transfer Transposition Activity attC Site
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Faelen M, Toussaint A (1976) Bacteriophage Mu-1: a tool to transpose and to localize bacterial genes. J Mol Biol 104(3):525–539PubMedCrossRefGoogle Scholar
Goldhaber-Gordon H, Early MH, Gray MK, Baker TA (2002) Sequence and positional requirement for DNA sites in a Mu transpososome. J Biol Chem 227:7703–7712CrossRefGoogle Scholar
Gray YHM (2000) It takes two transposons to tango. Transposable element-mediated chromosomal rearrangements. Trends Genet 16(10):461–468PubMedCrossRefGoogle Scholar
Haniford DB (2006) Transpososome dynamics and regulation in Tn10 transposition. Crit Rev Biochem Mol Biol 41:407–424PubMedCrossRefGoogle Scholar
Hayes F (2003) Transposon-based strategies for microbial functional genomics and proteomics. Annu Rev Genet 37:3–29PubMedCrossRefGoogle Scholar