Abstract
A total of 140 Fluorescent pseudomonads spp. were isolated from rhizosphere soil of groundnut. All isolates were evaluated in vitro for selection of potential antagonistic fluorescent pseudomonads (FPs) against fungal pathogens. Among 140 FPs, 82 FPs isolates showed antagonistic activity against Macrophomina phaseolina for primary screening with zone of inhibition range 0.9–2.9 cm. For secondary screening of other fungal pathogens such as Rhizoctonia solani, Fusarium oxysporum, Fusarium udum, and Alternaria alternata the zone of inhibition range was 0.7–1.9 cm, 0.7–2.9 cm, 0.9–2.9 cm, and 0.2–3.0 cm. All antagonistic FPs were screened for the production of indole acetic acid (59/82), hydrogen cyanide (61/82), siderophore (40/82), phosphate solubilization (66/82), hydrolytic enzymes such as protease (51/82), amylase (4/82), gelatinase (51/82), cellulase (48/82), and pectinase (3/82). Among 82 antagonistic bacteria, the strain designates as VSMKU-2013 were selected for further studies based on the antagonistic potential. The strain VSMKU-2013 was identified as Pseudomonas sp. based on the biochemical and morphological characters. The germination, growth promotion of groundnut seeds, and identification of IAA genes are in progress.
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The authors greatly acknowledged the Head, Department of Microbial Technology, the Co-ordinator, NRCBS, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamilnadu, India.
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Charulatha, R., Harikrishnan, H., Manoharan, P.T., Shanmugaiah, V. (2013). Characterization of Groundnut Rhizosphere Pseudomonas sp. VSMKU 2013 for Control of Phytopathogens. In: Velu, R. (eds) Microbiological Research In Agroecosystem Management. Springer, India. https://doi.org/10.1007/978-81-322-1087-0_8
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