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Androgenesis

  • Sant Saran Bhojwani
  • Prem Kumar Dantu
Chapter

Abstract

Haploid plants, characterized genetically by the presence of gametic number of chromosomes in their cells (i.e., half the number of chromosomes as in somatic cells), are of considerable importance in genetics and plant breeding programmes. However, there has been no reliable and reproducible method to produce haploids under field conditions. Therefore, the full potential of haploids could not be exploited in agriculture. Since the first report of androgenic haploid production in anther cultures of Datura innoxia by Guha and Maheshwari (1964, 1966), considerable success has been achieved in developing protocols for androgenic haploid production in anther/pollen culture of over 200 species, including several major crop plants. The technique is being routinely used in crop improvement programmes and has aided development of several improved varieties.

Keywords

Anther Culture Pollen Development Donor Plant Haploid Plant Pollen Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Suggested Further Reading

  1. Babbar SB, Narayan JP, Bhojwani SS (2000) Occurrence of albino plants in anther and pollen cultures: a problem limiting the application of in vitro androgenesis in crop improvement. Plant Tissue Cult 10:59–87Google Scholar
  2. Bhojwani SS, Dantu PK (2010) Haploid plants. In: Davey MR, Anthony P (eds) Plant cell culture: essential methods. Wiley-Blackwell, UKGoogle Scholar
  3. Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice, a revised edition. Elsevier, AmsterdamGoogle Scholar
  4. Binarova P, Hause G, Cenklova V, Cordewener JHG, Lookeren CMM (1997) A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. Sex Plant Reprod 10:200–2008CrossRefGoogle Scholar
  5. Blakeslee AF, Belling J, Farnham ME, Bergner AD (1922) A haploid mutant in the Jimson weed Datura stramonium. Science 55:646–647PubMedCrossRefGoogle Scholar
  6. Custers JBM, Cordewener JHG, Fiers MA, Maassen BTH, van Lookeren CMM, Liu CM (2001) Androgenesis in Brassica. In: Bhojwani SS, Soh WY (eds) Current trends in the embryology of angiosperms. Kluwer Academic Publishers, DordrechtGoogle Scholar
  7. Datta SK (2001) Androgenesis in cereals. In: Bhojwani SS, Soh WY (eds) Current trends in the embryology of angiosperms. Kluwer Academic Publishers, DordrechtGoogle Scholar
  8. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497CrossRefGoogle Scholar
  9. Hu H, Guo X (1999) In vitro induced haploids in plant genetics and breeding. In: Bhojwani SS, Soh WY (eds) Current trends in the embryology of angiosperms. Kluwer Academic Publishers, DordrechtGoogle Scholar
  10. Liu S, Wang H, Zhang J, Fitt BDL, Xu Z, Evans N, Liu Y, Yang W, Guo X (2005) In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotorum. Plant Cell Rep 24:133–144PubMedCrossRefGoogle Scholar
  11. Malik MR, Wang F, Dirpaul JM, Zhou N, Hammerlindl J, Keller W, Abrams SR, Ferrie AMR, Krochko JE (2008) Isolation of an embryogenic line from non-embryogenic Brassica napus cv Westar through microspore embryogenesis. J Exp Bot 59:2857–2873PubMedCrossRefGoogle Scholar
  12. Maluszynski M, Kasha KJ, Forster BP, Szarejko L (eds) (2003) Doubled haploid production in crop plants. Kluwer Academic Publishers, DordrechtGoogle Scholar
  13. Maraschin SF, de Priester W, Spaink HP, Wang M (2005a) Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot 5:1711–1726CrossRefGoogle Scholar
  14. Maraschin SF, Vennik M, Lamers GEM, Spaink HP, Wang M (2005b) Time-lapse tracking of barley androgenesis reveals position-determined cell deaths within proembryos. Planta 220:531–540CrossRefGoogle Scholar
  15. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87PubMedCrossRefGoogle Scholar
  16. Obert B, Barnabás B (2004) Colchicine-induced embryogenesis in maize. Plant Cell Tissue Organ Cult 77:283–285CrossRefGoogle Scholar
  17. Palmer C, Keller WA, Kasha KJ (2005) Haploids in crop improvement II. Springer, BerlinCrossRefGoogle Scholar
  18. Shim YS, Kasha KJ, Simion E, Letarte J (2006) The relationship between induction of embryogenesis and chromosome doubling in microspore culture. Protoplasma 228:79–86PubMedCrossRefGoogle Scholar
  19. Soriano M, Cistué L, Castillo AM (2008) Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture. Plant Cell Rep 27:805–811PubMedCrossRefGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational Institute (Deemed University)AgraIndia

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