Tissue and Cell Culture

  • Sant Saran Bhojwani
  • Prem Kumar Dantu


The pioneering attempts of G. Haberlandt (1902) to culture plant cells did not succeed but with the refinement of the in vitro techniques and tissue culture media and the discovery of plant growth regulators it has been possible to establish various types of tissue cultures, starting from embryonic, meristematic and mature tissues. Broadly, there are two types of tissue cultures: (i) unorganized (callus and suspension cultures), and (ii) organized (root cultures, shoot tip cultures, embryo cultures etc.). Organized tissue cultures are dealt with in some of the following chapters. This chapter describes unorganized tissue cultures.


Suspension Culture Hairy Root Cell Suspension Culture Callus Culture Pack Cell Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Suggested Further Reading

  1. Dong J, Bowra S, Vincze E (2010) The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application. BMC Plant Biol 10:239–250PubMedCrossRefGoogle Scholar
  2. Eibl R, Eibl D (2008) Design of bioreactors suitable for plant cell and tissue cultures. Phytochem Rev 7:593–598CrossRefGoogle Scholar
  3. Evans PK, Cocking EC (1975) The techniques of plant cell culture and somatic cell hybridization. In: Pain RH, Smith BJ (eds) New techniques in biophysics and cell biology, vol 2. John Wiley, LondonGoogle Scholar
  4. Kohlenbach HW (1966) The potentialities for development of explanted and isolated permanent cells. I. The extension and division growth of isolated mesophyll cells of Macleaya cordata. Z Pflanzenphysiol 55:142–157Google Scholar
  5. Matsubayashi Y, Sakagami Y (1996) Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc Natl Acad Sci USA 93:7623–7627PubMedCrossRefGoogle Scholar
  6. Reynolds JF, Murashige T (1979) Plant cell lines. In: Jakoby WB, Pastan IH (eds) Methods in enzymology, vol 58. Academic Press, New YorkGoogle Scholar
  7. Rossini L (1972) Division of free leaf cells of Calystegia sepium in vitro. Phytomorphology 22:21–29Google Scholar
  8. Vasil V, Hildebrandt AC (1965) Growth and tissue formation from single, isolated tobacco cells in microculture. Science 147:1454–1455PubMedCrossRefGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational InstituteAgraIndia

Personalised recommendations