Tissue and Cell Culture
The pioneering attempts of G. Haberlandt (1902) to culture plant cells did not succeed but with the refinement of the in vitro techniques and tissue culture media and the discovery of plant growth regulators it has been possible to establish various types of tissue cultures, starting from embryonic, meristematic and mature tissues. Broadly, there are two types of tissue cultures: (i) unorganized (callus and suspension cultures), and (ii) organized (root cultures, shoot tip cultures, embryo cultures etc.). Organized tissue cultures are dealt with in some of the following chapters. This chapter describes unorganized tissue cultures.
KeywordsSuspension Culture Hairy Root Cell Suspension Culture Callus Culture Pack Cell Volume
Suggested Further Reading
- Evans PK, Cocking EC (1975) The techniques of plant cell culture and somatic cell hybridization. In: Pain RH, Smith BJ (eds) New techniques in biophysics and cell biology, vol 2. John Wiley, LondonGoogle Scholar
- Kohlenbach HW (1966) The potentialities for development of explanted and isolated permanent cells. I. The extension and division growth of isolated mesophyll cells of Macleaya cordata. Z Pflanzenphysiol 55:142–157Google Scholar
- Reynolds JF, Murashige T (1979) Plant cell lines. In: Jakoby WB, Pastan IH (eds) Methods in enzymology, vol 58. Academic Press, New YorkGoogle Scholar
- Rossini L (1972) Division of free leaf cells of Calystegia sepium in vitro. Phytomorphology 22:21–29Google Scholar