General Requirements and Techniques

  • Sant Saran Bhojwani
  • Prem Kumar Dantu


A plant tissue culture laboratory, whether for research or for commercial purpose, should provide certain basic facilities, such as (i) washing and storage of glassware, plasticware and other labwares, (ii) preparation, sterilization and storage of nutrient media, (iii) aseptic manipulation of plant material, (iv) maintenance of cultures under controlled conditions of temperature, light and humidity, (v) observation of cultures and (vi) hardening of in vitro developed plants. The extent of sophistication in terms of equipment and facilities depends on the need and the funds available. Therefore, establishment of a new tissue culture facility requires careful planning. This chapter deals with the requirements to establish a tissue culture facility and the technique to initiate aseptic cultures.


Growth Room Clean Area Fire Extinguisher Cotton Plug Culture Vial 
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Suggested Further Reading

  1. Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice, a revised edition. Elsevier, AmsterdamGoogle Scholar
  2. Biondi S, Thorpe TA (1981) Requirements for a tissue culture facility. In: Thorpe TA (ed) Plant tissue culture methods and application in agriculture. Academic Press, New YorkGoogle Scholar
  3. Cassells AC (ed) (1997) Pathogen and contamination management in micropropagation. Kluwer, DordrechtGoogle Scholar
  4. Cassells AC (2001) Contamination and its impact on tissue culture. Acta Hortic 560:353–359Google Scholar
  5. Mageau OC (1991) Laboratory design. In: Debergh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer, DordrechtGoogle Scholar
  6. Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff, DordrechtCrossRefGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational InstituteAgraIndia

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