Clonal propagation of plants, which refers to multiplication of genetically identical individuals by asexual methods of regeneration from somatic tissues or organs, is a common practice in horticulture and forestry to preserve the desirable characters of selected genotypes or varieties in the progeny. Traditionally, it is achieved by cuttings, layering, splitting, grafting and so on. However, for many plants, especially the orchids and tree species, these methods are either very difficult or painfully slow. Since mid 1960s tissue culture has become an established industrial technology being widely used the world over to multiply orchids, other ornamentals, and fruit and forest tree species. In vitro clonal propagation of plants is popularly called micropropagation because of the miniaturization of the process. The major advantages of micropropagation over the conventional methods of clonal propagation are that it is much faster and goes on round the year, protected from pests, pathogens and vagaries of nature. This chapter describes the techniques of micropropagation, the factors that affect the various stages of micropropagation, and commercial aspects of micropropgation and discusses some of the common problems associated with it. The chapter is annexed with protocols and media composition for the micropropagation of selected crops.


Somatic Embryogenesis Clonal Propagation Shoot Multiplication Thin Cell Layer Orchid Seed 
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Suggested Further Reading

  1. Akita M, Shigeoka T, Koizumi Y, Kawamura M (1994) Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Rep 13:180–183Google Scholar
  2. Amoo SO, Finnie JF, Van Staden J (2011) The role of meta-topolins in alleviating micropropagation problems. Plant Growth Reg 63:197–206CrossRefGoogle Scholar
  3. Ascough GD, Erwin JE, van Staden J (2009) Microrpopagation of iridaceae—a review. Plant cell Tiss Organ Cult 97:1–19CrossRefGoogle Scholar
  4. Bairu MW, Stirk WA, Doležal K, Van Staden J (2008) The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tiss Organ Cult 95:373–379CrossRefGoogle Scholar
  5. Bairu MW, Nova’k O, Doležal K, Van Staden J (2011) Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis. Plant Growth Reg 63:105–114Google Scholar
  6. Bhagwat B, Lane WD (2003) Eliminating thrips from in vitro shoot cultures of apple with insecticides. HortScience 38:97–100Google Scholar
  7. Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice—a revised edition. Elsevier, AmsterdamGoogle Scholar
  8. Cha-um S, Chanseetis C, Chintakovid W, Pichakum A, Supaibulwatana K (2011) Promoting root induction and growth of in vitro macadamia (Macadamia tetraphylla L. ‘Keaau’) plantlets using CO2-enriched photoautotrophic conditions. Plant Cell Tiss Organ Cult 106:435–444CrossRefGoogle Scholar
  9. Cördük N, Aki C (2011) Inhibition of browning problem during micropropagation of Sideritis trojana, an endemic medicinal herb of Turkey. Romanian Biotechnol Lett 16:6760–6765Google Scholar
  10. Department of Biotechnology (2000) Plant tissue culture: from research to commercialization. A decade of support. Pub. DBT, Ministry of Science and Technology, Government of IndiaGoogle Scholar
  11. Donnelly DJ, Coleman WK, Coleman SE (2003) Potato microtuber production and performance: a review. Am J Potato Res 80:103–115CrossRefGoogle Scholar
  12. Etienne H, Dechamp E, Barry-Etienne D, Bertrand B (2006) Bioreactors in coffee micropropagation. Braz. J Plant Physiol 18:45–54CrossRefGoogle Scholar
  13. Iliev I, Kitin P (2011) Origin, morphology, and anatomy of fasciation in plants cultured in vivo and in vitro. Plant Growth Reg 63:115–129CrossRefGoogle Scholar
  14. Ka¨ma¨ra¨inen-Karppinen T, Virtanen E, Rokka V-M, Pirttila AM (2010) Novel bioreactor technology for mass propagation of potato microtubers. Plant Cell Tiss Organ Cult 101:245–249Google Scholar
  15. Kharrazi M, Nemati H, Tehranifar A, Bagheri A, Sharifi A (2011) In vitro culture of carnation (Dianthus caryophyllus L.) focusing on the problem of vitrification. J Biol Environ Sci 5:1–6Google Scholar
  16. Ko WH, Su CC, Chen CL, Chao CP (2009) Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant cell Tiss Organ Cult 96:137–141CrossRefGoogle Scholar
  17. Kozai TC, Kubota C (2002) Developing a photoautotrophic (sugar-free medium) micropropagation system for woody plants. J Plant Res 114:525–537CrossRefGoogle Scholar
  18. Kozai T, Xiao Y (2006) A commercialized photoautotrophic micropropagation system. In: Dutta Gupta S, Ibaraki Y (eds) Plant tissue culture engineering, Springer, DordrechtGoogle Scholar
  19. Kubota C (2002) Photoautotrophic micropropagation: importance of controlled environment in plant tissue culture. Comb Proc Int Plant Propagators’ Soc 52:609–613Google Scholar
  20. Leifert C, Cassells AC (2001) Microbial hazards in plant tissue and cell cultures. In Vitro Cell Dev Biol 37:133–138Google Scholar
  21. Mack Moyo M, Finnie JF, Van Staden J (2011) Recalcitrant effects associated with the development of basal callus-like tissue on caulogenesis and rhizogenesis in Sclerocarya birrea. Plant Growth Reg 63:187–195CrossRefGoogle Scholar
  22. Mark H, Brand MH (2011) Tissue proliferation condition in micropropagated ericaceous plants. Plant Growth Reg 63:131–136CrossRefGoogle Scholar
  23. Morel G (1965) Clonal propagation of orchids by meristem culture. Cymbidium Soc News 20:3–11Google Scholar
  24. Paek KY, Hahn EJ, Park SY (2011) Micropropagation of Phalaenopsis orchids via protocorms and protocorm-like bodies. In: Thorpe TA, Yeung WC (eds) Plant embryo culture: methods and protocols. Springer, New YorkGoogle Scholar
  25. Rani V, Raina SN (2000) Genetic fidelity of organized meristem-derived microplant: a critical reappraisal. In Vitro Cell Dev Biol Plant 36:319–330CrossRefGoogle Scholar
  26. Rout GR, Mohapatra A, Mohan Jain S (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24: 531–560Google Scholar
  27. Singh HP, Uma S, Selvarajan R, Karihaloo JL (2011) Micropropagation for production of quality banana planting material in Asia-Pacific. Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB), New DelhiGoogle Scholar
  28. Sluis CJ (2006) Integrating automation technologies with commercial micropropagation. In: Das Gupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, DordrechtGoogle Scholar
  29. Sreedhar RV, Venkatachalam L, Thimmaraju R, Bhagyalakshmi N, Narayan MS, Ravishankar GA (2008) Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biol Plant 52:355–360CrossRefGoogle Scholar
  30. Takayama S, Akita M (2006) Bioengineering aspects of bioreactor application in plant propagation. In: Das Gupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, DordrechtGoogle Scholar
  31. Teixeira da Silva JA, Tanaka M (2010) Thin cell layers: the technique. In: Davey MR, Anthony P (eds) Plant cell culture: essential Methods. John-Wiley, LondonGoogle Scholar
  32. Tomar UK, Negi U, Sinha AK, Dantu PK (2008) Economics and factors influencing cost of micropropagated plants. My Forest 44:135–147Google Scholar
  33. West TP, Preece JE (2006) Use of acephate, benomyl and alginate encapsulation for eliminating culture mites and fungal contamination from in vitro cultures of hardy hibiscus (Hibiscus moscheutos L.). In Vitro Cell Dev Biol Plant 42:301–304CrossRefGoogle Scholar
  34. Yam TW, Arditti J (2009) History of orchid propagation: a mirror of the history of biotechnology. Plant Biotechnol Rep 3:1–56CrossRefGoogle Scholar
  35. Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PG, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer Academic Publications, DordrechtGoogle Scholar
  36. Ziv M (2000) Bioreactor technology for plant micropropagation. Hortic Rev 24:1–30Google Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational Institute (Deemed University)AgraIndia

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