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Production of Virus-Free Plants

  • Sant Saran Bhojwani
  • Prem Kumar Dantu
Chapter

Abstract

Field grown plants, especially the perennial crops, harbor a variety of pathogens, such as fungi, bacteria, phytoplasma, viruses and viroids, which cause considerable economic losses and restrict international movement of germplasm. Whereas fungal and bacterial diseases can be controlled by the application of fungicides and bactericides, viruses and viroids generally do not respond to chemical control. Eradication of viruses is highly desirable to optimize the yields. Traditionally, thermotherapy of whole plant has been used to obtain virus free plants from infected stock plants, which is a cumbersome process. Moreover, not all viruses are eliminated by this method. Since 1952 shoot meristem-tip culture has become the most popular method for virus eradication. Where viruses invade the meristematic dome shoot-tip culture is combined with thermotherapy and /or chemotherapy. Cryotherapy of shoot-tip is emerging as a powerful technique for virus elimination. Recently electrotherapy of shoot tip before culture has been found helpful in elimination of some viruses. Shoot meristem-tip culture alone or in combination with thermotherapy, chemotherapy, electrotherapy and/or cryotherapy is being widely used to raise virus free plants from infected stock plants.

Keywords

Potato Virus Tomato Yellow Leaf Curl Virus Virus Elimination Meristem Culture Apple Stem Groove Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Suggested Further Reading

  1. Anonymous (1999) Virus eradication: tissue culture of meristems, thermotherapy, and chemotherapy: In: Techniques in plant virology. CIP training manual, Section 4.2. International Potato Centre, LimaGoogle Scholar
  2. Borroto-Fernandez EG, Sommerbauer T, Popowich E, Schartl A, Laimer M (2009) Somatic embryogenesis from anthers of the autochthonous Vitis vinifera cv. Domina leads to Arabis mosaic virus-free plants. Eur J Plant Pathol 124:171–174CrossRefGoogle Scholar
  3. Cieślińska M (2007) Application of thermo- and chemotherapy in vitro for eliminating some viruses infecting Prunus sp. fruit trees. J Fruit Ornam Plant Res 15:117–124Google Scholar
  4. El Far MMM, Ashoub A (2009) Utility of thermotherapy and meristem-tip culture for freeing sweet potato from viral infection. Aust J Basic Appl Sci 3:153–159Google Scholar
  5. Falah M, Mozafari J, Sokhandan BN, Hashemi M (2009) Elimination of a DNA virus associated with yellow leaf curl disease in tomato using an electrotherapy technique. Acta Hortic 808:157–162Google Scholar
  6. Gambino G, Bondaz J, Gribaudo I (2006) Detection and elimination of viruses in callus, somatic embryos and regenerated plantlets of grapevine. Euro J Plant Pathol 114:397–404CrossRefGoogle Scholar
  7. Komar V, Vigne E, Demangeat E, Fuchs M (2007) Beneficial effect of selected virus elimination on the performance of Vitis vinifera cv Chardonnay. Am J Enol Vitic 58:202–210Google Scholar
  8. Krizan B, Ondrusikova E, Holleinova V, Moravcova K, Blahova L (2009) Elimination of grapevine fanleaf virus in grapevine by in vitro and in vivo thermotherapy. Hortic Sci (Prague) 36:105–108Google Scholar
  9. Lim ST, Wong SM, Goh CJ (2008) Elimination of cymbidium mosaic virus and Odontoglossum ringspot virus from orchids by meristem culture and thin section culture with chemotherapy. Ann Appl Biol 122:289–297CrossRefGoogle Scholar
  10. Lopez-Delgado H, Dat JF, Foyer H, Scott IM (1998) Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2. J Exp Bot 49:713–720Google Scholar
  11. Lopez-Delgado H, Mora-Herrera ME, Zavaleta-Mancera HA, Cadena-Hinojosa M, Lozoya-Saldafia H, Abello EJ, de la Garcia GR (1996) Electrotherapy and shoot tip culture eliminate Potato Virus X in potatoes. Am Potato J 73:149–154CrossRefGoogle Scholar
  12. Meybodi DE, Mozafari J, Babaeiyan N, Rahimian H (2011) Application of electrotherapy for the elimination of potato potyvirus. J Agric Sci Tech 13:921–927Google Scholar
  13. Rowhani A, Uyemoto JK, Golino A, Martelli GP (2005) Pathogen testing and certification of Vitis and Prunus species. Ann Rev Phytopathol 43:261–268Google Scholar
  14. Wang QC, Valkonen JPT (2009) Cryotherapy of shoot tips: novel pathogen eradication method. Trends Plant Sci 14:119–122PubMedCrossRefGoogle Scholar
  15. Wang QC, Cuellar WJ, Rajamäki M-L, Hirata Y, Valkonen JPT (2008) Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol Plant Pathol 9:237–250PubMedCrossRefGoogle Scholar
  16. Wang Q, Liu Y, Xie Y, You M (2006) Cryotherapy of potato shoot tips for efficient elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY). Potato Res 49:119–129CrossRefGoogle Scholar
  17. Wang QC, Panis B, Engelmann F, Lambardi M, Valkonen JPT (2009) Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Ann Appl Biol 154:351–363CrossRefGoogle Scholar
  18. Waterworth HE, Hadidi A (1998) Economic losses due to plant viruses. In: Hadidi A et al. (eds) Plant virus disease control. APS Press, MinnesotaGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational Institute (Deemed University)AgraIndia

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